FastQCFastQC Report
Wed 25 May 2016
SRR1033059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152413
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31372.058223379895416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11540.7571532612047529No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7400.48552288846751923No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5560.364798278362082No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3500.22963920400490773No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3300.21651696377605587No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3290.21586085176461323No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3090.2027386115357614No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3090.2027386115357614No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3050.200114163489991No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2800.18371136320392617No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2740.17977469113527061No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2720.17846246711238542No Hit
GGTTATGGCTTTTGAGAGATAGGTTTCTTTGCTTCTTTAGTTGGGTTGGC2450.16074744280343542No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2120.1390957464258298No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2000.1312224022885187No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1980.1299101782656335No Hit
ATATAAGGAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACA1930.12662961820842056No Hit
GTATTAGACTTGCACTTTTTTAAAAAAAGGTTTCTGCATCGTGGAAGCAT1890.12400517016265017No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.12269294613976497No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1840.12072461010543721No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1790.11744405004822424No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1780.11678793803678164No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.10104124976215939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG200.002162031870.478081
ACTAGAC200.002162031870.478083
GGTTATG409.714313E-870.478081
ATCGGTC250.00523378356.382463
TAGGATC250.00523378356.382464
TCGGTCA250.00523378356.382464
TACATGA950.054.404132
TTATGGC558.779025E-751.2567833
TATGGCT708.563802E-846.9853864
ACATGAG405.6698103E-446.9853863
GTACAAA1750.045.642951
GAACAAG751.4732723E-743.8530271
GTTATGG704.618616E-640.273192
TAACAAC1500.037.588311
AGGCTAT500.00170273537.57596694
GAGAACA2050.036.671521
TATTAAG702.180124E-433.560992
CGCAGCT352.1894537E-433.54997390-91
CGCAGAA1703.6379788E-1233.1661572
GCCCGAA300.004105977231.41127228-29
Produced by FastQC (version 0.11.5)