##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033059_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152413
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.80892706002769	31.0	30.0	34.0	15.0	34.0
2	28.770931613445047	33.0	30.0	34.0	12.0	34.0
3	29.009362718403285	33.0	30.0	34.0	15.0	34.0
4	31.79708423822115	37.0	33.0	37.0	12.0	37.0
5	32.467328902390214	37.0	35.0	37.0	17.0	37.0
6	32.14468582076332	37.0	35.0	37.0	13.0	37.0
7	32.408580632885645	37.0	35.0	37.0	13.0	37.0
8	31.972699179203875	37.0	35.0	37.0	10.0	37.0
9	33.04859821668755	39.0	35.0	39.0	8.0	39.0
10-11	33.42976648973513	39.0	35.0	39.0	11.0	39.0
12-13	33.42253613536903	39.0	35.0	39.0	10.0	39.0
14-15	34.624664562734154	40.0	35.0	41.0	8.0	41.0
16-17	34.820126892063016	40.0	35.0	41.0	12.0	41.0
18-19	34.856869164703795	40.0	35.0	41.0	13.0	41.0
20-21	34.89022261880548	40.0	34.5	41.0	16.5	41.0
22-23	34.9003037798613	40.0	34.0	41.0	19.0	41.0
24-25	34.89842729950857	40.0	34.0	41.0	22.5	41.0
26-27	34.56264557485254	40.0	33.5	41.0	19.5	41.0
28-29	34.34275619533767	40.0	33.5	41.0	12.5	41.0
30-31	34.21059555287279	40.0	34.0	41.0	2.0	41.0
32-33	33.95314048014277	40.0	33.0	41.0	2.0	41.0
34-35	33.65675500121381	40.0	33.0	41.0	2.0	41.0
36-37	33.35865706993498	40.0	33.0	41.0	2.0	41.0
38-39	33.09314166114439	40.0	33.0	41.0	2.0	41.0
40-41	32.9238647621922	40.0	33.0	41.0	2.0	41.0
42-43	32.67239343100654	40.0	33.0	41.0	2.0	41.0
44-45	32.50759449653245	40.0	32.0	41.0	2.0	41.0
46-47	32.30386187529935	40.0	32.0	41.0	2.0	41.0
48-49	32.095572556146784	39.5	31.0	41.0	2.0	41.0
50-51	31.92518682789526	39.0	31.0	41.0	2.0	41.0
52-53	31.760752035587515	39.0	31.0	41.0	2.0	41.0
54-55	31.592485549132945	39.0	30.0	41.0	2.0	41.0
56-57	31.39229593276164	38.5	30.0	41.0	2.0	41.0
58-59	31.168486283978403	38.0	30.0	41.0	2.0	41.0
60-61	30.90524758386752	38.0	30.0	40.5	2.0	41.0
62-63	30.670490706173357	37.0	30.0	40.0	2.0	41.0
64-65	30.384461955345017	37.0	29.5	40.0	2.0	41.0
66-67	30.050504222080793	36.0	29.0	40.0	2.0	41.0
68-69	29.69235891951474	35.5	29.0	39.0	2.0	41.0
70-71	29.307378635680685	35.0	28.0	39.0	2.0	41.0
72-73	28.98288531818152	35.0	28.0	38.5	2.0	40.5
74-75	28.660029000150907	35.0	28.0	37.0	2.0	40.0
76-77	28.343461515749972	35.0	27.0	37.0	2.0	39.0
78-79	27.975264577168616	35.0	27.0	36.0	2.0	39.0
80-81	27.623103016146914	35.0	26.0	36.0	2.0	39.0
82-83	27.40727825054293	35.0	26.0	35.5	2.0	37.0
84-85	27.193198742889386	35.0	25.5	35.0	2.0	37.0
86-87	27.057744418127065	35.0	25.0	35.0	2.0	37.0
88-89	26.820241711665016	34.5	25.0	35.0	2.0	36.0
90-91	26.560956086423076	34.0	24.5	35.0	2.0	36.0
92-93	26.284985532730147	34.0	24.0	35.0	2.0	36.0
94-95	26.029895743801383	34.0	21.5	35.0	2.0	35.5
96-97	25.788758176795945	34.0	18.5	35.0	2.0	35.0
98-99	25.574914869466514	34.0	9.5	35.0	2.0	35.0
100	25.43191853713266	34.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7146285416717184
1101	2	0.6576259618046052
1101	3	0.7026145199454419
1101	4	0.4812414149096895
1101	5	-0.26005675698228714
1101	6	0.5273208974786812
1101	7	0.9607741705342434
1101	8	0.6313048569867519
1101	9	0.881333565222512
1101	10-11	0.4911996029010055
1101	12-13	0.6698809816703104
1101	14-15	1.139151963803144
1101	16-17	0.7753784338675942
1101	18-19	1.03436738604875
1101	20-21	1.0285007128294765
1101	22-23	0.6924880635013224
1101	24-25	0.6430585585561843
1101	26-27	0.3878249048787268
1101	28-29	0.4978442217144874
1101	30-31	0.9146305181937464
1101	32-33	1.1708673975677613
1101	34-35	1.6083059394833512
1101	36-37	1.7258979343571283
1101	38-39	1.9145077910697097
1101	40-41	1.8907627161986653
1101	42-43	1.889963258855488
1101	44-45	1.9832837295867947
1101	46-47	1.82914895240323
1101	48-49	1.6469446802614556
1101	50-51	1.526874906762938
1101	52-53	1.5186446413880468
1101	54-55	1.457411848041989
1101	56-57	1.6320331957639027
1101	58-59	1.5949581929329533
1101	60-61	1.8687923290243837
1101	62-63	1.8723687498673662
1101	64-65	1.8531570777807822
1101	66-67	1.869588084380247
1101	68-69	2.01095335097029
1101	70-71	1.8444498516327101
1101	72-73	1.6165512784888776
1101	74-75	1.6037227808608243
1101	76-77	1.5533789516676784
1101	78-79	1.3748625841598106
1101	80-81	1.535863825476607
1101	82-83	1.3791011243917595
1101	84-85	1.4913504143351908
1101	86-87	1.655876366702934
1101	88-89	1.841128772583673
1101	90-91	1.7551312170448448
1101	92-93	1.2682393428389496
1101	94-95	1.6676867006835678
1101	96-97	1.9333933982642648
1101	98-99	1.8328071710357499
1101	100	1.9635772074450877
1102	1	0.6483163992174426
1102	2	0.21533014895041447
1102	3	0.5356927032774337
1102	4	-0.20613268870807033
1102	5	0.07166510265835058
1102	6	-0.10738487428292487
1102	7	0.36563007792822333
1102	8	0.12036026482388706
1102	9	0.2272363933588437
1102	10-11	0.4632539632802377
1102	12-13	-0.21115197296670374
1102	14-15	-0.4118377496231176
1102	16-17	-0.011638459748276375
1102	18-19	-0.21353098367703183
1102	20-21	-0.45948037921252904
1102	22-23	-0.19841516750343402
1102	24-25	0.3813149440206942
1102	26-27	0.5241018634907064
1102	28-29	0.7300038650416383
1102	30-31	0.4913769528081957
1102	32-33	0.8874226039706912
1102	34-35	0.6198011184912247
1102	36-37	0.7884902583707714
1102	38-39	0.7578994060349302
1102	40-41	0.779960470878656
1102	42-43	0.6211023021772704
1102	44-45	0.349195689407388
1102	46-47	0.3196681155112273
1102	48-49	0.3531595971362691
1102	50-51	0.28644303181328823
1102	52-53	0.2869818691161363
1102	54-55	0.26469302334347233
1102	56-57	0.4806755291778053
1102	58-59	0.18173605128441395
1102	60-61	0.2725067758860398
1102	62-63	0.42373447382192
1102	64-65	0.2722073826178928
1102	66-67	0.1920708281827892
1102	68-69	0.09687175668565118
1102	70-71	-0.27453062267771244
1102	72-73	-0.17951672570094956
1102	74-75	-0.05530281471299503
1102	76-77	-0.02522067769337255
1102	78-79	0.19106058056085118
1102	80-81	-0.051760056563519896
1102	82-83	0.20699573401225635
1102	84-85	0.1923817574985094
1102	86-87	0.21159306962776014
1102	88-89	0.27462862486525097
1102	90-91	0.14136117434079054
1102	92-93	0.2037132503940775
1102	94-95	0.2816716065466487
1102	96-97	0.2889919264516294
1102	98-99	0.6493946069125265
1102	100	0.5424454157549192
1103	1	0.1574309499913653
1103	2	0.07397334366922692
1103	3	0.22824852833807086
1103	4	0.16980189657361677
1103	5	0.4547763026783542
1103	6	0.5085287062726991
1103	7	0.301040545283378
1103	8	0.4581612700500628
1103	9	0.034042924828426635
1103	10-11	0.09337073955489927
1103	12-13	0.08066368555646619
1103	14-15	0.23667066477011645
1103	16-17	-0.44173523152192473
1103	18-19	-0.056583170419045814
1103	20-21	-0.09456077230097293
1103	22-23	0.06988269951664705
1103	24-25	0.16467870086598424
1103	26-27	0.5404430402554894
1103	28-29	0.21723849283289098
1103	30-31	0.31036122426927903
1103	32-33	-0.048800546238112474
1103	34-35	-0.05936419370402035
1103	36-37	-0.07359120995960211
1103	38-39	-0.07436285297335132
1103	40-41	-0.01108001323026997
1103	42-43	-0.19286834121748342
1103	44-45	-0.24997662436321377
1103	46-47	-0.2645383309392244
1103	48-49	-0.26146619366665647
1103	50-51	-0.2927747921971111
1103	52-53	-0.31505358886190393
1103	54-55	-0.45054290474034886
1103	56-57	-0.27710747199746066
1103	58-59	-0.08648679885686761
1103	60-61	-0.14324926922411052
1103	62-63	-0.07919118445119366
1103	64-65	0.03341615459158831
1103	66-67	-0.07166351204376298
1103	68-69	-0.07835516937271336
1103	70-71	-0.18519802284275144
1103	72-73	-0.20829820153301526
1103	74-75	-0.327676598814417
1103	76-77	-0.22850391642208834
1103	78-79	-0.02287505495714015
1103	80-81	0.3051577499429463
1103	82-83	0.07450342324233361
1103	84-85	0.1640378801261022
1103	86-87	0.2018533781971854
1103	88-89	0.39075475324413134
1103	90-91	0.47324834895507806
1103	92-93	0.685134070078508
1103	94-95	0.5213630371185225
1103	96-97	0.17522882975487164
1103	98-99	0.3058047992057915
1103	100	0.20404427549836157
1104	1	0.7410057592254056
1104	2	1.2170719624270738
1104	3	1.3838314511623757
1104	4	1.5080854858714048
1104	5	0.4845744288253684
1104	6	0.8486716600059196
1104	7	1.0087822626599845
1104	8	0.8532158436036212
1104	9	1.4849750218051483
1104	10-11	1.0616166586121807
1104	12-13	1.2452778070671329
1104	14-15	1.654363604014776
1104	16-17	1.2896952717138461
1104	18-19	1.4298700315513955
1104	20-21	1.541378086000968
1104	22-23	0.6657607058916071
1104	24-25	0.6571808741490841
1104	26-27	0.4250175597184409
1104	28-29	-0.08914612998174931
1104	30-31	0.1586781372413597
1104	32-33	0.05991190425933013
1104	34-35	0.48206099706194294
1104	36-37	0.18757705044801298
1104	38-39	0.4428302311568544
1104	40-41	0.6267182095071036
1104	42-43	0.6013700437916825
1104	44-45	0.7291598578158656
1104	46-47	0.8996049125650742
1104	48-49	0.9137124984998621
1104	50-51	0.8121223390691874
1104	52-53	0.7318410310550298
1104	54-55	0.6639166721938743
1104	56-57	0.9673506560813578
1104	58-59	1.1264707979749637
1104	60-61	1.2158978378945982
1104	62-63	1.2781810747973346
1104	64-65	1.3989865206690517
1104	66-67	0.9014119250276202
1104	68-69	1.3521842877894628
1104	70-71	1.4292615540914717
1104	72-73	1.24621981256918
1104	74-75	1.1828155290712168
1104	76-77	1.2636964119851406
1104	78-79	1.1808460886727268
1104	80-81	1.2764100439640025
1104	82-83	1.1924764745318157
1104	84-85	1.1843942985555422
1104	86-87	1.1492237056385086
1104	88-89	1.2158175369783173
1104	90-91	1.3089360723202859
1104	92-93	1.2908039242270597
1104	94-95	1.3397610859932492
1104	96-97	1.2512816646819473
1104	98-99	1.2549982696974347
1104	100	1.2892852684472658
1105	1	-1.3235405244043257
1105	2	-1.5432107553111827
1105	3	-1.1305096341089431
1105	4	-1.3877877471427311
1105	5	-1.4105920098431248
1105	6	-1.7813784995928046
1105	7	-1.3615095750053143
1105	8	-1.434426655256388
1105	9	-1.4688325477342943
1105	10-11	-1.6867873541557223
1105	12-13	-1.4310787820801494
1105	14-15	-1.5842022874580834
1105	16-17	-1.2299125714234052
1105	18-19	-1.1224555498439557
1105	20-21	-1.2304755391700297
1105	22-23	-1.2965853953395765
1105	24-25	-1.1974360889152038
1105	26-27	-1.2179828962779098
1105	28-29	-2.3084803753077843
1105	30-31	-2.6120582968671684
1105	32-33	-2.5202978540626013
1105	34-35	-2.5318810914013525
1105	36-37	-2.427824226341773
1105	38-39	-2.4460434578536336
1105	40-41	-2.400242574806626
1105	42-43	-2.2263773342292907
1105	44-45	-2.208459841226542
1105	46-47	-2.016525958387959
1105	48-49	-2.045943224254355
1105	50-51	-1.8170158278254753
1105	52-53	-1.774132521156556
1105	54-55	-1.9403240938799975
1105	56-57	-1.954160971825619
1105	58-59	-1.9501137893483325
1105	60-61	-1.9004952309608534
1105	62-63	-2.0403665768214516
1105	64-65	-2.06464777025662
1105	66-67	-2.0177627215752096
1105	68-69	-2.0413291641029225
1105	70-71	-2.040095491052167
1105	72-73	-1.8191814642206054
1105	74-75	-2.089608091539816
1105	76-77	-2.145218316697033
1105	78-79	-2.184691987022667
1105	80-81	-2.0105370604591606
1105	82-83	-2.0171336485871976
1105	84-85	-2.3088341556168253
1105	86-87	-2.1764602888895332
1105	88-89	-2.2476001556801393
1105	90-91	-2.0999444566354803
1105	92-93	-1.883797078964914
1105	94-95	-1.862644294763708
1105	96-97	-2.1123184265173265
1105	98-99	-2.021517918173064
1105	100	-1.8300489768787713
1106	1	-0.2933079662647913
1106	2	-0.46814266396402004
1106	3	-0.5553577380268138
1106	4	-0.6897152560469841
1106	5	-0.7186582155837478
1106	6	-0.2509573807995942
1106	7	0.40557611533618854
1106	8	-0.20746248178058835
1106	9	-0.16950298561403798
1106	10-11	-0.6727500610486601
1106	12-13	-0.6083257965364695
1106	14-15	-0.8242399995888263
1106	16-17	-0.4168495083603432
1106	18-19	-0.5791389774576103
1106	20-21	-0.3833039489751897
1106	22-23	-0.43631931530605783
1106	24-25	-0.8491121836145581
1106	26-27	-0.9275261604723397
1106	28-29	-0.947386723516459
1106	30-31	-0.799655196091976
1106	32-33	-1.1439831672827978
1106	34-35	-0.9329496017721866
1106	36-37	-0.7637345552753629
1106	38-39	-0.8124691859072684
1106	40-41	-0.9385308630949112
1106	42-43	-0.8889585200662893
1106	44-45	-1.0376657913627234
1106	46-47	-0.9752626020083213
1106	48-49	-1.1894274061106316
1106	50-51	-1.11898523909721
1106	52-53	-1.0796322068887996
1106	54-55	-1.2224108317806888
1106	56-57	-0.8963880826573813
1106	58-59	-0.7715154235406665
1106	60-61	-0.8141763539443012
1106	62-63	-0.6459710184724052
1106	64-65	-0.6222111475874392
1106	66-67	-0.7354455206533572
1106	68-69	-0.9284860884691497
1106	70-71	-0.9638227066398493
1106	72-73	-0.9258125138749129
1106	74-75	-0.9710662939282493
1106	76-77	-1.1281954799067542
1106	78-79	-0.9750230157257889
1106	80-81	-1.3003652607524785
1106	82-83	-1.034669789379155
1106	84-85	-0.7981176251328499
1106	86-87	-0.5233385625119951
1106	88-89	-0.6883292568743613
1106	90-91	-0.6748344629208383
1106	92-93	-0.2739814493818393
1106	94-95	-0.4375971546002866
1106	96-97	-0.2311640162931461
1106	98-99	-0.24791640968783213
1106	100	0.07969332356120518
1107	1	0.3941920337352087
1107	2	-0.13122985830121436
1107	3	-0.3274780726471498
1107	4	-0.025703029534756894
1107	5	0.020201179525649593
1107	6	-0.361237303579518
1107	7	-0.03839910988903483
1107	8	0.36179427497616956
1107	9	-0.13205929192034205
1107	10-11	0.34321679866819466
1107	12-13	0.012390902305234874
1107	14-15	-0.250398300747122
1107	16-17	-0.44419828570911335
1107	18-19	0.05487003155139547
1107	20-21	-0.06839082156205478
1107	22-23	-0.007164714276456152
1107	24-25	0.199854193476817
1107	26-27	0.2927521535559734
1107	28-29	0.5250432957885636
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2216	92-93	-1.4061748672895469
2216	94-95	-1.3808771693088744
2216	96-97	-1.3082921648498669
2216	98-99	-1.043421098049663
2216	100	-1.141487040476303
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6277.0
3	842.0
4	637.0
5	783.0
6	1862.0
7	3937.0
8	4121.0
9	2383.0
10	1502.0
11	1310.0
12	1282.0
13	862.0
14	667.0
15	557.0
16	519.0
17	461.0
18	436.0
19	359.0
20	337.0
21	357.0
22	474.0
23	965.0
24	1935.0
25	1567.0
26	1441.0
27	958.0
28	1153.0
29	1341.0
30	1504.0
31	1850.0
32	2470.0
33	3391.0
34	4931.0
35	8466.0
36	12872.0
37	25272.0
38	37001.0
39	15324.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38811526264993	22.47805505767728	13.291051978491659	18.84277770118112
2	15.11913357400722	28.91696750902527	34.41220872989826	21.551690187069248
3	19.1032332184474	34.39422287871328	27.118989003774825	19.3835548990645
4	11.799933022083014	24.307074049997045	34.50741681933692	29.385576108583024
5	11.159855256158508	46.2635204802028	29.47546775771825	13.101156505920445
6	24.723395034571517	42.9448497304538	16.526038622917667	15.805716612057022
7	22.097306952279013	42.929846796383	19.278176528916937	15.69466972242105
8	21.071953974675566	41.68418010823307	18.1986549676877	19.045210949403668
9	20.44856007618297	28.07408137129347	18.493416083801268	32.98394246872229
10-11	19.619490442931774	36.798242589619065	23.56414121015699	20.018125757292168
12-13	21.656519483248935	36.18472471249647	23.318161816378673	18.840593987875923
14-15	20.045585767256412	37.44338727211222	22.208939145628133	20.302087815003237
16-17	17.786831159536856	39.14836202000571	23.679882342513288	19.384924477944146
18-19	18.1462668238369	38.37033291413974	24.993022560933564	18.490377701089795
20-21	20.763247495624938	35.70445911736991	24.516444657497367	19.01584872950779
22-23	20.60065974102703	35.62240493656967	23.95614855661136	19.820786765791937
24-25	20.90662991583403	36.19727321947138	23.7538350478253	19.142261816869286
26-27	20.042470103282557	35.02041326171708	25.379831302964362	19.557285332036003
28-29	19.536641256459248	37.39732503397877	24.60555084996159	18.460482859600397
30-31	19.61350182710018	37.045317994228135	24.26612296893109	19.075057209740596
32-33	18.89505773581065	37.02118019417093	24.776329138444467	19.307432931573953
34-35	18.894266261996552	37.19924534492659	24.67000246083176	19.2364859322451
36-37	18.715517439437054	37.60629900585199	24.65677441800166	19.021409136709302
38-39	18.86867400267878	36.86904167870368	24.83323266013604	19.429051658481498
40-41	18.33119931711481	37.39715683377655	24.861288945795987	19.410354903312648
42-43	18.176088241087257	37.96697524784978	24.310288227956143	19.546648283106823
44-45	18.421752361068712	37.00862360445	24.75240374357005	19.817220290911237
46-47	18.180087131521322	37.039432430391635	25.23366940580505	19.546811032282
48-49	18.628265272958274	36.104882684902186	24.775858729444824	20.49099331269472
50-51	17.079143728477494	35.646028041218166	25.789254258542528	21.485573971761816
52-53	18.252431009238162	35.37914551912963	24.56484770490404	21.803575766728166
54-55	18.16191410930171	34.739022396706964	24.445990881129713	22.65307261286161
56-57	18.08044799831935	33.27096545476031	24.72623783826368	23.922348708656664
58-59	19.08261638804001	32.94414227520852	24.69957229467354	23.273669042077934
60-61	18.613452549761043	32.808479071477336	24.810277821542986	23.767790557218632
62-63	17.74762176747484	32.319671216328885	24.73164871092904	25.20105830526724
64-65	17.840824043801945	32.78810677380812	24.534210422657267	24.83685875973267
66-67	18.713768948470662	31.272772631482198	24.18149828323081	25.831960136816328
68-69	18.852824936647366	31.19936713015848	24.476438071979096	25.47136986121506
70-71	18.949258356008812	30.86085846235652	25.15976774185078	25.03011543978389
72-73	18.76011330180226	29.910428557830855	26.270132240139425	25.059325900227456
74-75	18.497900986979225	30.956093045850057	25.444173474646085	25.101832492524622
76-77	19.21924385993127	29.667945608328765	25.88232205042028	25.230488481319686
78-79	19.62939502643662	29.638223642969436	26.812081748394274	23.92029958219967
80-81	20.385230110829962	29.373057521964157	26.521081322345513	23.720631044860372
82-83	19.685770738015375	29.471711489356117	27.295672209057315	23.546845563571196
84-85	20.206226842963705	29.887139701949046	26.395657553172985	23.51097590191426
86-87	20.3832358124317	29.46531417263661	26.57939557825027	23.572054436681423
88-89	20.01286431105379	28.95290415103751	26.64881415336753	24.385417384541167
90-91	21.03510061563907	28.89171840747693	26.3805935863273	23.6925873905567
92-93	21.243149223852186	29.52085589576975	26.048702044566962	23.1872928358111
94-95	20.40397742189551	29.68265949067997	26.247046468889472	23.66631661853505
96-97	19.7576728189241	30.43890624589777	26.664194386830843	23.139226548347292
98-99	19.392600809296738	31.40143546793826	25.1971553186811	24.008808404083897
100	18.937404820669013	31.88310665336344	25.09124087591241	24.088247650055138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2191.0
1	1385.5
2	544.5
3	462.0
4	357.0
5	246.5
6	196.0
7	201.0
8	208.5
9	212.5
10	179.0
11	141.5
12	119.0
13	103.5
14	109.5
15	124.5
16	154.5
17	158.0
18	160.0
19	195.0
20	244.0
21	302.5
22	375.0
23	485.0
24	605.0
25	870.0
26	1265.5
27	1580.0
28	1944.5
29	2350.5
30	2655.5
31	2839.0
32	3158.5
33	3915.0
34	4452.0
35	4646.0
36	4735.5
37	4755.5
38	4885.5
39	5019.5
40	5430.0
41	5548.0
42	5404.0
43	5522.0
44	5535.0
45	5560.0
46	5645.5
47	6176.0
48	7327.5
49	6997.5
50	5715.5
51	4664.0
52	3844.5
53	3510.5
54	2904.0
55	2495.0
56	2271.0
57	1848.5
58	1454.5
59	1144.5
60	917.5
61	848.0
62	700.5
63	506.0
64	468.5
65	522.0
66	470.5
67	333.5
68	236.5
69	187.5
70	171.5
71	209.0
72	183.0
73	87.0
74	37.5
75	19.5
76	15.5
77	11.5
78	11.0
79	9.0
80	9.5
81	7.5
82	3.5
83	1.5
84	2.0
85	2.0
86	1.5
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06561120114425935
2	0.04133505672088339
3	0.05773785700694823
4	0.08135788941888158
5	0.09448012964773346
6	0.07873344137311122
7	0.08660678551042233
8	0.09776068970494642
9	0.09710457769350384
10-11	0.09415207364201217
12-13	0.10104124976215939
14-15	0.11317932197384738
16-17	0.06987592921863621
18-19	0.08955928956191402
20-21	0.08496650548181586
22-23	0.05281701692112878
24-25	0.023291976406212066
26-27	0.2007702755014336
28-29	0.07348454528157047
30-31	0.08037372140171771
32-33	0.08168594542460289
34-35	0.016730856291786136
36-37	0.04691200881814543
38-39	0.0688917612014723
40-41	0.07742121735022603
42-43	0.06757953717858713
44-45	0.06462703312709546
46-47	0.07512482531017695
48-49	0.07184426525296399
50-51	0.06659536916142324
52-53	0.07217232125868528
54-55	0.06003424904699731
56-57	0.059706193041276
58-59	0.05806591301266952
60-61	0.055769520972620445
62-63	0.061674529075603786
64-65	0.059706193041276
66-67	0.060362305052718605
68-69	0.059706193041276
70-71	0.054129240944013966
72-73	0.0501925688753584
74-75	0.05216090490968618
76-77	0.04822423284103062
78-79	0.044287560772375056
80-81	0.04100700071516209
82-83	0.04724006482386673
84-85	0.037070328646506535
86-87	0.03444588060073615
88-89	0.03510199261217875
90-91	0.03346171258357227
92-93	0.038054496663670424
94-95	0.03477393660645745
96-97	0.03739838465222783
98-99	0.038382552669391716
100	0.04527172878953895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	152413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.11457684055822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43393403422525	39.99593210552906
2	7.534365523598327	7.400943489072455
3	2.936265145544171	4.326402603452461
4	1.685881079925725	3.3120534337622116
5	1.247712304794475	3.064043093436912
6	0.8790093110864715	2.590330221175359
7	0.6425584781650346	2.209129142527212
8	0.5103063173784682	2.0050783069685654
9	0.43816877513125024	1.9368426577785358
>10	2.544852184832414	22.019775216024883
>50	0.09751927007494289	3.2149488560687085
>100	0.04408405359552213	4.258823066273874
>500	0.002671760823971038	0.850321166829601
>1k	0.002671760823971038	2.8153766411001686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3137	2.058223379895416	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1154	0.7571532612047529	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	740	0.48552288846751923	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	556	0.364798278362082	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	350	0.22963920400490773	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	330	0.21651696377605587	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	329	0.21586085176461323	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	309	0.2027386115357614	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	309	0.2027386115357614	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	305	0.200114163489991	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	280	0.18371136320392617	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	274	0.17977469113527061	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	272	0.17846246711238542	No Hit
GGTTATGGCTTTTGAGAGATAGGTTTCTTTGCTTCTTTAGTTGGGTTGGC	245	0.16074744280343542	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	212	0.1390957464258298	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	200	0.1312224022885187	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	198	0.1299101782656335	No Hit
ATATAAGGAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACA	193	0.12662961820842056	No Hit
GTATTAGACTTGCACTTTTTTAAAAAAAGGTTTCTGCATCGTGGAAGCAT	189	0.12400517016265017	No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	187	0.12269294613976497	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	184	0.12072461010543721	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	179	0.11744405004822424	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	178	0.11678793803678164	No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	154	0.10104124976215939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0013122240228851869	0.0
16-17	0.0	0.0	0.0	0.0013122240228851869	0.0
18-19	0.0	0.0	0.0	0.0026244480457703737	0.0
20-21	0.0	0.0	0.0	0.003280560057212967	0.0
22-23	0.0	0.0	0.0	0.007545288131589825	0.0
24-25	0.0	0.0	0.0	0.01738696830322873	0.0
26-27	0.0	0.0	0.0	0.030509208532080598	0.0
28-29	0.0	0.0	0.0	0.07348454528157047	0.0
30-31	0.0	0.0	0.0	0.15713882674050114	0.0
32-33	0.0	0.0	0.0	0.3070604213551338	0.0
34-35	0.0	0.0	0.0	0.5307946172570581	0.0
36-37	0.0	0.0	0.0	0.8450722707380605	0.0
38-39	0.0	0.0	0.0	1.239395589615059	0.0
40-41	0.0	0.0	0.0	1.6822711973388098	0.0
42-43	0.0	0.0	0.0	1.999829410877025	0.0
44-45	0.0	0.0	0.0	2.2803172957687337	0.0
46-47	0.0	0.0	0.0	2.6296969418619147	0.0
48-49	0.0	0.0	0.0	2.9646421237033587	0.0
50-51	0.0	0.0	0.0	3.300243417556245	0.0
52-53	0.0	0.0	0.0	3.6141930150315265	0.0
54-55	0.0	0.0	0.0	3.9327353965869056	0.0
56-57	0.0	0.0	0.0	4.314264531240774	0.0
58-59	0.0	0.0	0.0	4.678078641585692	0.0
60-61	0.0	0.0	0.0	5.026146063655988	0.0
62-63	0.0	0.0	0.0	5.378806269806381	0.0
64-65	0.0	0.0	0.0	5.754102340351545	0.0
66-67	0.0	0.0	0.0	6.165812627531772	0.0
68-69	0.0	0.0	0.0	6.6473988439306355	0.0
70-71	0.0	0.0	0.0	7.1575259328272525	0.0
72-73	0.0	0.0	0.0	7.665028573678098	0.0
74-75	0.0	0.0	0.0	8.21452238326127	0.0
76-77	0.0	0.0	0.0	8.794853457382246	0.0
78-79	0.0	0.0	0.0	9.46638410109374	0.0
80-81	0.0	0.0	0.0	10.13463418474802	0.0
82-83	0.0	0.0	0.0	10.808461220499563	0.0
84-85	0.0	0.0	0.0	11.489505488376977	0.0
86-87	0.0	0.0	0.0	12.202043132803633	0.0
88	0.0	6.561120114425934E-4	0.0	12.73775859014651	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGG	20	0.0021620318	70.47808	1
ACTAGAC	20	0.0021620318	70.47808	3
GGTTATG	40	9.714313E-8	70.47808	1
ATCGGTC	25	0.005233783	56.38246	3
TAGGATC	25	0.005233783	56.38246	4
TCGGTCA	25	0.005233783	56.38246	4
TACATGA	95	0.0	54.40413	2
TTATGGC	55	8.779025E-7	51.256783	3
TATGGCT	70	8.563802E-8	46.985386	4
ACATGAG	40	5.6698103E-4	46.985386	3
GTACAAA	175	0.0	45.64295	1
GAACAAG	75	1.4732723E-7	43.853027	1
GTTATGG	70	4.618616E-6	40.27319	2
TAACAAC	150	0.0	37.58831	1
AGGCTAT	50	0.001702735	37.575966	94
GAGAACA	205	0.0	36.67152	1
TATTAAG	70	2.180124E-4	33.56099	2
CGCAGCT	35	2.1894537E-4	33.549973	90-91
CGCAGAA	170	3.6379788E-12	33.166157	2
GCCCGAA	30	0.0041059772	31.411272	28-29
>>END_MODULE