FastQCFastQC Report
Wed 25 May 2016
SRR1033059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152413
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25201.6534022688353356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23751.5582660271761595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.8778778713101901No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12560.8240766863718975No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11150.7315648927584917No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7210.4730567602501099No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.42450447140335795No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.41663112726604684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.3798888546252616No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5580.3661105023849672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.3090287573894615No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT4390.28803317302329856TruSeq Adapter, Index 1 (95% over 21bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3590.23554421210789106No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3430.22504641992480956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3160.20733139561585953No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3070.2014263875128762No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3030.1988019394671058No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3000.19683360343277803No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2880.18896025929546692No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2750.1804308031467132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2740.17977469113527061No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT2600.1705891229750743RNA PCR Primer, Index 1 (95% over 23bp)
GGTTATGGCTTTTGAGAGATAGGTTTCTTTGCTTCTTTAGTTGGGTTGGC2400.15746688274622245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2290.1502496506203539No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2260.14828131458602614No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2170.14237630648304278No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2140.140407970448715No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC2120.1390957464258298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2110.13843963441438722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.1312224022885187No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1970.12925406625419092No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.119412386082552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1780.11678793803678164No Hit
ATATAAGGAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACA1780.11678793803678164No Hit
GTATTAGACTTGCACTTTTTTAAAAAAAGGTTTCTGCATCGTGGAAGCAT1740.11416348999101127No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1730.11350737797956867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1690.1108829299337983No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.1095707059109131No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1670.1095707059109131No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1580.10366569780792977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA652.7699098E-643.3824929
GATAGCC652.7699098E-643.3824927
CTTAGGT450.001011429441.7757383
GCCTTAG500.001676016837.6971051
AGGTCCT500.001697806637.5981646
ATAGCCG757.4037252E-637.598168
CCTTAGG651.3919109E-436.2233852
GGTCCTG550.00271030334.180157
CACAGGA550.00271030334.180157
GGATAGC851.7454433E-533.1748476
CTATAAT600.004097680631.4142531
ATGGATA902.580165E-531.3318044
ATGGGAG1052.0506523E-631.33185
TAGGTCC600.00415076231.33185
TTGTCCA753.2632652E-431.33186
GTCCACG650.006139125728.9216638
GTTAGGT856.712907E-427.7002372
CTTAGAT856.712907E-427.7002372
CTGTGTG856.790742E-427.6457069
ACTGCTC350.00882812726.8558392-93
Produced by FastQC (version 0.11.5)