##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033059_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152413
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.491362285369355	33.0	31.0	34.0	31.0	34.0
2	32.42187346223747	34.0	31.0	34.0	31.0	34.0
3	32.46106303268094	34.0	31.0	34.0	30.0	34.0
4	36.08578664549612	37.0	35.0	37.0	35.0	37.0
5	36.11855287934756	37.0	35.0	37.0	35.0	37.0
6	36.23822771023469	37.0	37.0	37.0	35.0	37.0
7	36.182832173108594	37.0	37.0	37.0	35.0	37.0
8	36.206406277679726	37.0	37.0	37.0	35.0	37.0
9	37.94705176067658	39.0	38.0	39.0	35.0	39.0
10-11	37.915056458438585	39.0	38.0	39.0	35.0	39.0
12-13	37.88453084710622	39.0	38.0	39.0	35.0	39.0
14-15	39.11382231174506	41.0	38.5	41.0	36.0	41.0
16-17	39.15831654780104	40.5	38.5	41.0	36.0	41.0
18-19	39.338042030535455	41.0	39.0	41.0	36.0	41.0
20-21	39.36137993478246	41.0	39.0	41.0	36.0	41.0
22-23	39.20956873757488	41.0	39.0	41.0	35.5	41.0
24-25	39.07380275960712	41.0	39.0	41.0	35.0	41.0
26-27	38.831412018659826	40.5	38.0	41.0	35.0	41.0
28-29	38.674732470327335	40.0	38.0	41.0	35.0	41.0
30-31	38.32384376660784	40.0	37.5	41.0	35.0	41.0
32-33	37.83061484256592	40.0	35.5	41.0	34.0	41.0
34-35	37.463070735435956	40.0	35.0	41.0	33.0	41.0
36-37	37.128709493284695	40.0	35.0	41.0	33.0	41.0
38-39	36.79251441806145	40.0	35.0	41.0	33.0	41.0
40-41	36.48654642320537	40.0	35.0	41.0	31.5	41.0
42-43	36.01956197962116	40.0	35.0	41.0	30.5	41.0
44-45	35.31640673695813	40.0	35.0	41.0	25.0	41.0
46-47	34.279270797110485	40.0	35.0	41.0	2.0	41.0
48-49	33.328456234048275	40.0	35.0	41.0	2.0	41.0
50-51	32.81270626521359	40.0	34.5	41.0	2.0	41.0
52-53	32.606024420489064	39.0	34.0	41.0	2.0	41.0
54-55	32.40669431085274	39.0	34.0	41.0	2.0	41.0
56-57	32.20327662338514	39.0	34.0	41.0	2.0	41.0
58-59	31.99499058479264	39.0	33.0	41.0	2.0	41.0
60-61	31.713285612119698	38.0	33.0	41.0	2.0	41.0
62-63	31.47398843930636	37.5	33.0	41.0	2.0	41.0
64-65	31.156308845046027	37.0	32.5	40.0	2.0	41.0
66-67	30.726899936357135	36.5	32.0	40.0	2.0	41.0
68-69	30.271709106178605	36.0	31.0	39.0	2.0	41.0
70-71	29.759036302677593	35.0	30.5	39.0	2.0	41.0
72-73	29.356495180857276	35.0	30.0	38.5	2.0	41.0
74-75	29.040462427745666	35.0	30.0	37.0	2.0	40.0
76-77	28.718885528137363	35.0	29.0	37.0	2.0	39.0
78-79	28.383274392604307	35.0	29.0	36.0	2.0	39.0
80-81	28.091229094631036	35.0	29.0	36.0	2.0	39.0
82-83	27.825831786002507	35.0	28.5	35.5	2.0	37.0
84-85	27.63516563547729	35.0	28.5	35.0	2.0	37.0
86-87	27.49220538930406	35.0	28.0	35.0	2.0	36.5
88-89	27.300197489715444	35.0	27.0	35.0	2.0	36.0
90-91	27.107628614357044	35.0	27.0	35.0	2.0	36.0
92-93	26.92851659635333	35.0	26.0	35.0	2.0	36.0
94-95	26.76797254827344	35.0	25.5	35.0	2.0	35.0
96-97	26.627275888539693	35.0	25.0	35.0	2.0	35.0
98-99	26.500761089933274	35.0	25.0	35.0	2.0	35.0
100	26.382697014034235	35.0	24.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18139999010617913
1101	2	0.11443057366271603
1101	3	0.29969847148792184
1101	4	0.34144287721642286
1101	5	0.19468332735809923
1101	6	-0.0467308542543563
1101	7	0.13287797783188182
1101	8	0.13643328153099787
1101	9	0.3064512446295069
1101	10-11	0.3113545233807429
1101	12-13	0.27870625158623596
1101	14-15	0.44815561441165386
1101	16-17	0.4731164604055209
1101	18-19	0.4786134782282261
1101	20-21	0.4007776288443665
1101	22-23	0.5504135282791651
1101	24-25	0.3564750944370587
1101	26-27	0.5378030863827448
1101	28-29	0.6385154866092293
1101	30-31	0.45051818941471566
1101	32-33	0.49685218369452855
1101	34-35	0.5054149791175035
1101	36-37	0.5975145211155635
1101	38-39	0.6156522391251329
1101	40-41	1.0890318981144418
1101	42-43	1.4017066197777126
1101	44-45	1.1699123773898847
1101	46-47	1.085801796984832
1101	48-49	1.5647353676640137
1101	50-51	1.4097343880754707
1101	52-53	1.40614779105222
1101	54-55	1.3544565512757956
1101	56-57	1.357544683643468
1101	58-59	1.2212769018670642
1101	60-61	1.834774961343438
1101	62-63	1.4916267468129547
1101	64-65	1.5233613415636462
1101	66-67	1.8205809892906757
1101	68-69	2.1354926963850147
1101	70-71	1.8215476225392067
1101	72-73	1.8922581803600096
1101	74-75	1.7626334833789237
1101	76-77	1.5032820186515572
1101	78-79	1.5877464623486794
1101	80-81	1.5726414312830599
1101	82-83	1.837661437359646
1101	84-85	1.5590727336325827
1101	86-87	1.5177138382040312
1101	88-89	1.867695185997814
1101	90-91	2.0480213132825824
1101	92-93	1.7224456611582823
1101	94-95	1.809426736293176
1101	96-97	2.072628823314137
1101	98-99	1.7939229880116763
1101	100	1.8888525442556414
1102	1	0.22388917980349277
1102	2	0.11617633682979545
1102	3	0.3381052611637472
1102	4	0.18655339745384936
1102	5	0.07940924254086212
1102	6	0.16251900474169645
1102	7	-0.032727801987100236
1102	8	-0.04061396150609653
1102	9	0.30099909196923136
1102	10-11	0.31194539706806523
1102	12-13	0.19007519848818077
1102	14-15	0.16034641289687102
1102	16-17	0.32961472807129866
1102	18-19	0.26996792186693597
1102	20-21	0.14659451171709037
1102	22-23	0.27846391368995427
1102	24-25	0.424506142163537
1102	26-27	0.028268533700980925
1102	28-29	0.38164657999413265
1102	30-31	0.47655691849913495
1102	32-33	0.18718065574888954
1102	34-35	0.19415883537404
1102	36-37	-0.032517976937242565
1102	38-39	-0.4998769956934339
1102	40-41	-0.40150069394098864
1102	42-43	-0.062464948454767466
1102	44-45	-0.0037104840502379943
1102	46-47	-0.1265635778170875
1102	48-49	-0.04836322766809076
1102	50-51	-0.04009506429205345
1102	52-53	0.0336556469864675
1102	54-55	-0.0548711956154051
1102	56-57	-0.13223588735537106
1102	58-59	0.07676799847491367
1102	60-61	0.09351049165257663
1102	62-63	-0.06292163768466708
1102	64-65	-0.1496706461891506
1102	66-67	-0.1530042710966164
1102	68-69	-0.3277522744741681
1102	70-71	-0.3336775809093453
1102	72-73	-0.5509239430251576
1102	74-75	-0.4594544493689092
1102	76-77	-0.29480032765414066
1102	78-79	-0.2635816605530472
1102	80-81	-0.34583142881416506
1102	82-83	-0.35345934259362366
1102	84-85	-0.2925911129551224
1102	86-87	-0.2756683764979826
1102	88-89	-0.16492372733175742
1102	90-91	-0.22545920131468478
1102	92-93	0.11869361324379923
1102	94-95	0.154201532844624
1102	96-97	-0.27406631272110005
1102	98-99	-0.28846360452720177
1102	100	-0.2063318351926995
1103	1	0.04912485783104614
1103	2	0.2072968389427743
1103	3	-0.1104456627941417
1103	4	0.14366874840781207
1103	5	0.06788164883227665
1103	6	0.04139175110962867
1103	7	0.1644415611196024
1103	8	0.1805858084421459
1103	9	0.23438410359684525
1103	10-11	-0.022617379556677975
1103	12-13	-0.059005844746550906
1103	14-15	0.05050919952385868
1103	16-17	-0.2496531071916266
1103	18-19	-0.009344923523272541
1103	20-21	0.0109764976639255
1103	22-23	0.20494737246818318
1103	24-25	0.05452289593313964
1103	26-27	0.288852165018028
1103	28-29	0.2269117646606773
1103	30-31	-0.2546451019554681
1103	32-33	-0.1054101771885314
1103	34-35	0.30563391726747113
1103	36-37	0.032471645727859766
1103	38-39	0.02597882186551459
1103	40-41	-0.4386090433196017
1103	42-43	-0.3847374597698163
1103	44-45	0.022220350387513577
1103	46-47	0.32982660997516433
1103	48-49	0.20394354135494552
1103	50-51	0.1427210693380161
1103	52-53	0.11961248727553198
1103	54-55	0.28184206486820784
1103	56-57	0.5405404873289328
1103	58-59	0.790516091795908
1103	60-61	0.43612507993494276
1103	62-63	0.3534220396427301
1103	64-65	0.7264264756632635
1103	66-67	0.7687656271304846
1103	68-69	0.9275926781401722
1103	70-71	0.96248905658409
1103	72-73	0.9979870619509619
1103	74-75	1.177338829118753
1103	76-77	0.8954549797900349
1103	78-79	0.7829663127409425
1103	80-81	0.6907768080391286
1103	82-83	0.7741693738675828
1103	84-85	0.9476879498340054
1103	86-87	0.6826472445167475
1103	88-89	0.6663633122521482
1103	90-91	0.46965970043821414
1103	92-93	0.5597928605744507
1103	94-95	0.44297900777648636
1103	96-97	0.1260862215991203
1103	98-99	0.001914230485677848
1103	100	-0.031417479462660935
1104	1	-0.17916645869556191
1104	2	0.10594940341683667
1104	3	-0.15159315627429493
1104	4	0.08366198708259276
1104	5	0.12566683187101546
1104	6	-0.023855037260894107
1104	7	0.12836506155425553
1104	8	0.19821354919361767
1104	9	0.21385865203691168
1104	10-11	0.26463027812708617
1104	12-13	0.16969598081126236
1104	14-15	0.24075598789437436
1104	16-17	-0.060613698324637255
1104	18-19	0.1728244959686549
1104	20-21	0.2248517029184427
1104	22-23	0.09507583764735728
1104	24-25	0.24987689960357073
1104	26-27	0.22458335404536456
1104	28-29	0.2126284651339745
1104	30-31	0.3870650303733285
1104	32-33	0.461877082542955
1104	34-35	0.6789677335526036
1104	36-37	0.3697289624476454
1104	38-39	-0.24028458004698194
1104	40-41	0.08074525012191458
1104	42-43	0.1320209677391162
1104	44-45	0.4471454928582972
1104	46-47	1.1430691483698325
1104	48-49	1.0419373595718895
1104	50-51	1.1868507901918761
1104	52-53	1.079116978727285
1104	54-55	1.1803980389819912
1104	56-57	0.7966981227969114
1104	58-59	0.7693628028941433
1104	60-61	1.2367746501078365
1104	62-63	1.1189610138531023
1104	64-65	1.3077917804058536
1104	66-67	1.567857677743831
1104	68-69	1.5393597987815326
1104	70-71	1.193979928795045
1104	72-73	0.8906630978825767
1104	74-75	1.291267159071424
1104	76-77	0.9240336526384851
1104	78-79	1.1103110437367896
1104	80-81	1.0569629376633927
1104	82-83	1.1288612506182822
1104	84-85	1.1162622761162453
1104	86-87	1.0787160168532672
1104	88-89	0.9406799354531508
1104	90-91	1.0770440334817764
1104	92-93	1.0155906969503832
1104	94-95	1.2529374739215626
1104	96-97	1.4605061964856283
1104	98-99	1.374221774192815
1104	100	1.4223881807324545
1105	1	0.23118465849550063
1105	2	0.1835827823498093
1105	3	0.16988427190628386
1105	4	0.07438246131879822
1105	5	-0.12533636010345361
1105	6	-0.04104609973239093
1105	7	0.10251006839420285
1105	8	0.24176120081240526
1105	9	0.1262617528121055
1105	10-11	0.35264897397752293
1105	12-13	0.20546889462794837
1105	14-15	0.30271449222634317
1105	16-17	0.24694444121024617
1105	18-19	0.35321665283140646
1105	20-21	0.37640209051533446
1105	22-23	0.38882868762456013
1105	24-25	0.18115824935276947
1105	26-27	0.2384798428734598
1105	28-29	0.10846749842171022
1105	30-31	0.028640497769593765
1105	32-33	-0.01601849429243174
1105	34-35	-0.11566290940224633
1105	36-37	-0.28331481320214635
1105	38-39	0.20824483171772812
1105	40-41	0.18175984200517803
1105	42-43	-0.07066941073785671
1105	44-45	-0.8279343065033089
1105	46-47	-1.1803234644892697
1105	48-49	-0.9918291702958726
1105	50-51	-1.1827228532189835
1105	52-53	-1.4810061402877608
1105	54-55	-1.5848322391757854
1105	56-57	-1.3265576911565766
1105	58-59	-1.131731588350732
1105	60-61	-1.2755486513057512
1105	62-63	-1.2510458260921737
1105	64-65	-1.000632752197884
1105	66-67	-1.0838979082114761
1105	68-69	-1.1207519203247145
1105	70-71	-1.215698593776775
1105	72-73	-1.4914504452090789
1105	74-75	-1.5138400000713048
1105	76-77	-1.548914363777385
1105	78-79	-1.5596387966644834
1105	80-81	-1.7863225160529765
1105	82-83	-1.7338040480593264
1105	84-85	-1.6398936746361485
1105	86-87	-1.7472301755771937
1105	88-89	-1.7869990431150207
1105	90-91	-1.5425410098378798
1105	92-93	-1.571402007107988
1105	94-95	-1.5906101776972221
1105	96-97	-1.5313086689954503
1105	98-99	-1.535718946281424
1105	100	-1.5201790787294698
1106	1	-0.3826640739578835
1106	2	-0.12523766600552477
1106	3	-0.30633613454668307
1106	4	-0.18780065202710716
1106	5	-0.10340047072570258
1106	6	0.001317193793695992
1106	7	-0.11408326912936673
1106	8	-0.15578751068979813
1106	9	0.07421901239727191
1106	10-11	-0.03896007693385428
1106	12-13	-0.1583379354579506
1106	14-15	-0.3103171440611021
1106	16-17	-0.35228036499130155
1106	18-19	-0.16660316698841626
1106	20-21	-0.2728960448293094
1106	22-23	-0.09172861386297626
1106	24-25	-0.041458553496589445
1106	26-27	-0.16657986800021263
1106	28-29	-0.2048993568056119
1106	30-31	0.09341108230761819
1106	32-33	-0.08826865142631135
1106	34-35	-0.2842675605650413
1106	36-37	-0.0791979055968639
1106	38-39	-0.07779120431830933
1106	40-41	-0.48582867674612373
1106	42-43	-0.9367389686678749
1106	44-45	-0.7615190540415497
1106	46-47	-0.9128397016566723
1106	48-49	-1.3475697946411493
1106	50-51	-1.4268879485468702
1106	52-53	-1.3757054908010673
1106	54-55	-1.4397662429469946
1106	56-57	-1.3361847600859704
1106	58-59	-1.63061070002054
1106	60-61	-1.535416658848181
1106	62-63	-1.4450956899094827
1106	64-65	-1.0646909964886895
1106	66-67	-0.869609200899518
1106	68-69	-0.5894822785899549
1106	70-71	-0.5205802195886342
1106	72-73	-0.5515070134051818
1106	74-75	-0.732182761437322
1106	76-77	-0.6416128762433395
1106	78-79	-0.5873572127549949
1106	80-81	-0.7040995954579614
1106	82-83	-0.5578769581787455
1106	84-85	-0.3793657048058563
1106	86-87	-0.44499887450868414
1106	88-89	-0.30322573492310667
1106	90-91	-0.6419543766931071
1106	92-93	-0.8448359061232793
1106	94-95	-0.6633317364652953
1106	96-97	-1.0125992619139481
1106	98-99	-0.9369864928978053
1106	100	-0.7974051420020452
1107	1	0.20537485783104614
1107	2	0.21991999165213372
1107	3	0.3392156672551181
1107	4	0.13889658092013235
1107	5	0.03909470301947238
1107	6	0.06586835209485287
1107	7	0.14412136407526788
1107	8	0.017716350314067597
1107	9	0.2793348425131015
1107	10-11	0.07590478793100885
1107	12-13	0.1899165690465523
1107	14-15	0.2849611699672039
1107	16-17	0.18045773024679335
1107	18-19	0.302770224259973
1107	20-21	0.1526790838708152
1107	22-23	0.3246364118770444
1107	24-25	0.05182893041589409
1107	26-27	0.1668540123086686
1107	28-29	0.19927937549811503
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2216	76-77	-0.9292576638881265
2216	78-79	-1.039048700160766
2216	80-81	-1.0630350615362865
2216	82-83	-0.9248102179691529
2216	84-85	-1.0372991484535845
2216	86-87	-0.9989150355676983
2216	88-89	-1.2556986159674146
2216	90-91	-1.2667935015322342
2216	92-93	-1.3605097432256912
2216	94-95	-1.4853578442056872
2216	96-97	-1.4124429831862386
2216	98-99	-1.1238774655030603
2216	100	-1.2917974935372563
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	0.0
4	4.0
5	21.0
6	64.0
7	140.0
8	201.0
9	221.0
10	350.0
11	625.0
12	1146.0
13	1654.0
14	1796.0
15	2371.0
16	3261.0
17	4689.0
18	5506.0
19	2215.0
20	939.0
21	670.0
22	716.0
23	743.0
24	875.0
25	1167.0
26	1429.0
27	1681.0
28	1169.0
29	1439.0
30	1688.0
31	2061.0
32	2619.0
33	3423.0
34	4586.0
35	7907.0
36	12511.0
37	25865.0
38	40101.0
39	16536.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.81800874440345	19.959823011173405	14.950172656014074	18.27199558840907
2	16.59925684785111	23.148860609647826	38.84383940021703	21.408043142284043
3	19.305566490827495	29.414350576070124	33.128625042647556	18.151457890454818
4	11.46396514618655	20.105899952758385	38.34313159414204	30.087003306913022
5	10.11731359735454	39.70225441566281	35.13174815631315	15.048683830669502
6	24.760355087820592	39.85289968703457	17.555589090169473	17.831156134975362
7	20.59273159113724	38.25001804308032	23.300505862360822	17.856744503421623
8	24.721806681888562	32.12083038080991	21.401858121407763	21.75550481589376
9	23.957930097826303	16.12264045717885	23.257858581617054	36.6615708633778
10-11	22.680703227774806	27.034377327170194	28.068091974686947	22.21682747036805
12-13	26.80578428349288	25.818991818283216	29.593932276118178	17.781291622105726
14-15	22.74018620458885	25.973506196977947	31.58063944676635	19.705668151666853
16-17	16.499576807752618	28.94963028088155	33.11331710549625	21.43747580586958
18-19	16.560758210502488	24.839004930730294	39.7499532515591	18.85028360720812
20-21	18.21268526962923	22.529902304921496	40.89382139318825	18.363591032261027
22-23	17.672377028206256	23.353650935287675	40.98731735481881	17.98665468168726
24-25	18.60534543223728	23.853015376237202	40.91122330810541	16.630415883420106
26-27	17.813827606003443	22.89215123431477	42.200606905601575	17.093414254080212
28-29	17.194071371864606	23.9625228819064	41.70149527927408	17.14191046695492
30-31	17.906995817272207	23.666365947674894	41.08685311244156	17.339785122611335
32-33	17.68615538044655	22.94259676011889	41.600125973506195	17.771121885928366
34-35	17.328902390216058	23.268028317794414	41.908170562878496	17.494898729111032
36-37	17.923339872583046	23.141398699585995	41.33964950496349	17.59561192286747
38-39	17.84362226319277	22.820559925990565	41.15200146969091	18.18381634112576
40-41	17.453292872959896	23.263511851062084	40.550479783482324	18.732715492495693
42-43	18.29141871100234	23.813257399303208	39.0091396403194	18.886184249375056
44-45	18.623411388792295	23.414669352351833	38.46784723087925	19.494072027976618
46-47	19.41665081062639	23.86148163214424	37.200566880777885	19.521300676451485
48-49	20.2994495220224	23.939230905500185	35.14463989292252	20.616679679554895
50-51	18.728061254617387	24.366687880955038	35.61605637314402	21.289194491283553
52-53	19.625950542276577	25.01984738834614	34.57251021894458	20.781691850432708
54-55	19.191932446707302	25.199622079481408	33.473850655783956	22.134594818027335
56-57	18.89504176152953	24.477242754883115	33.26094230807083	23.366773175516524
58-59	18.96426157873672	24.697040278716383	33.90491624730174	22.433781895245154
60-61	19.043651132121276	25.897397203650605	33.06804537670671	21.990906287521405
62-63	17.65827061996024	26.071594942688613	33.302605420797434	22.967529016553705
64-65	17.512285697414264	27.080039104275883	32.8830217894799	22.524653408829955
66-67	18.36916798435829	25.680552183868834	32.2961295952445	23.65415023652838
68-69	18.814729762978757	25.64881489379152	32.386123185434265	23.150332157795457
70-71	18.965901858765328	25.702531936252154	32.774107195580434	22.557459009402084
72-73	19.102435823833346	24.72533420815222	33.34601820716805	22.826211760846387
74-75	18.745509718691054	25.96506192077421	31.948495038136638	23.340933322398097
76-77	19.558371005097992	24.375873449115232	32.149488560687075	23.916266985099696
78-79	19.47963756372488	24.672764134293008	32.81577030830704	23.03182799367508
80-81	19.62102970219076	24.431642970087854	32.65797536955509	23.2893519581663
82-83	18.62872139752317	24.806364307389487	33.05896826047732	23.505946034610023
84-85	19.2119438630563	25.55097006160892	31.77189609810187	23.465189977232914
86-87	19.453520720153268	25.44812744403328	31.32397711466289	23.774374721150565
88-89	18.830438134606236	25.154930039532175	31.249446385512524	24.765185440349065
90-91	19.847711406225397	25.244409742270747	30.77692772032964	24.130951131174218
92-93	20.09822093477198	25.434760500355946	30.355977075220704	24.11104148965137
94-95	19.479437314647143	24.985237382883238	30.81679920216256	24.718526100307063
96-97	19.485998477810146	24.806117628533187	31.46701047161641	24.24087342204026
98-99	19.481733721754193	24.93963729890035	29.933666640422015	25.644962338923445
100	19.490463411913684	24.447389658362475	29.817010360008663	26.245136569715182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	123.5
2	235.0
3	413.0
4	264.0
5	131.0
6	248.5
7	502.0
8	772.0
9	870.0
10	618.5
11	303.5
12	235.0
13	228.0
14	220.0
15	236.0
16	252.5
17	267.0
18	302.5
19	335.0
20	361.5
21	380.5
22	437.0
23	533.0
24	704.0
25	1095.5
26	1530.0
27	1870.0
28	2266.0
29	2699.0
30	3109.0
31	3442.5
32	3843.0
33	4606.5
34	5069.0
35	5131.5
36	5111.5
37	4920.0
38	4748.0
39	4620.5
40	4918.5
41	5130.0
42	4984.5
43	5151.5
44	5156.0
45	5200.5
46	5389.5
47	5993.0
48	7440.0
49	7140.0
50	5905.0
51	4849.0
52	3500.0
53	2911.5
54	2475.5
55	2124.5
56	2008.0
57	1728.5
58	1317.0
59	1016.5
60	815.0
61	709.0
62	576.0
63	431.0
64	387.5
65	447.0
66	405.5
67	261.5
68	181.5
69	138.0
70	138.0
71	188.5
72	161.5
73	55.0
74	18.0
75	12.0
76	8.0
77	4.0
78	3.0
79	5.0
80	4.0
81	1.5
82	1.5
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05708174499550563
2	0.23488810009644848
3	6.561120114425934E-4
4	0.0032805600572129675
5	6.561120114425934E-4
6	0.0
7	0.0
8	6.561120114425934E-4
9	0.0
10-11	9.841680171638902E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.841680171638902E-4
20-21	0.0
22-23	0.0
24-25	0.0016402800286064838
26-27	3.280560057212967E-4
28-29	0.0
30-31	3.280560057212967E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	3.280560057212967E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.280560057212967E-4
70-71	0.0
72-73	3.280560057212967E-4
74-75	3.280560057212967E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.280560057212967E-4
84-85	0.0
86-87	6.561120114425934E-4
88-89	0.0036086160629342644
90-91	0.0032805600572129675
92-93	9.841680171638902E-4
94-95	6.561120114425934E-4
96-97	6.561120114425934E-4
98-99	6.561120114425934E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	152413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.84559716034721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98053137147814	31.85161370749214
2	8.531178719439716	6.969221785543228
3	3.4969640505027786	4.285067546731578
4	2.1492594853342757	3.5115114852407605
5	1.4360523018601214	2.9328206911483927
6	1.085874000064253	2.661190318411159
7	0.7790664053715424	2.2275002788476046
8	0.6232531242972339	2.0365716835178103
9	0.5188421627525942	1.907317617263619
>10	3.1483920711922124	23.360212055402098
>50	0.15581328107430847	4.20961466541568
>100	0.07870980177980531	6.341978702604109
>500	0.008031612426510746	2.0601917159297436
>1k	0.008031612426510746	5.645187746452074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2520	1.6534022688353356	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2375	1.5582660271761595	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1338	0.8778778713101901	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1256	0.8240766863718975	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	1115	0.7315648927584917	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	721	0.4730567602501099	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.42450447140335795	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.41663112726604684	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.3798888546252616	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	558	0.3661105023849672	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	471	0.3090287573894615	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	439	0.28803317302329856	TruSeq Adapter, Index 1 (95% over 21bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	359	0.23554421210789106	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	343	0.22504641992480956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	316	0.20733139561585953	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	307	0.2014263875128762	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	303	0.1988019394671058	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	300	0.19683360343277803	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	288	0.18896025929546692	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	275	0.1804308031467132	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	274	0.17977469113527061	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	260	0.1705891229750743	RNA PCR Primer, Index 1 (95% over 23bp)
GGTTATGGCTTTTGAGAGATAGGTTTCTTTGCTTCTTTAGTTGGGTTGGC	240	0.15746688274622245	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	229	0.1502496506203539	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	226	0.14828131458602614	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	217	0.14237630648304278	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	214	0.140407970448715	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	212	0.1390957464258298	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	211	0.13843963441438722	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	200	0.1312224022885187	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	197	0.12925406625419092	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.119412386082552	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	178	0.11678793803678164	No Hit
ATATAAGGAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACA	178	0.11678793803678164	No Hit
GTATTAGACTTGCACTTTTTTAAAAAAAGGTTTCTGCATCGTGGAAGCAT	174	0.11416348999101127	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	173	0.11350737797956867	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	169	0.1108829299337983	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.1095707059109131	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	167	0.1095707059109131	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	158	0.10366569780792977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0013122240228851869	0.0
16-17	0.0	0.0	0.0	0.0013122240228851869	0.0
18-19	0.0	0.0	0.0	0.0026244480457703737	0.0
20-21	0.0	0.0	0.0	0.003280560057212967	0.0
22-23	0.0	0.0	0.0	0.007545288131589825	0.0
24-25	0.0	0.0	0.0	0.01738696830322873	0.0
26-27	0.0	0.0	0.0	0.030509208532080598	0.0
28-29	0.0	0.0	0.0	0.07479676930445565	0.0
30-31	0.0	0.0	0.0	0.15779493875194373	0.0
32-33	0.0	0.0	0.0	0.30771653336657634	0.0
34-35	0.0	0.0	0.0	0.5307946172570581	0.0
36-37	0.0	0.0	0.0	0.8499931108238798	0.0
38-39	0.0	0.0	0.0	1.244972541712321	0.0
40-41	0.0	0.0	0.0	1.6908006534875635	0.0
42-43	0.0	0.0	0.0	2.0096710910486637	0.0
44-45	0.0	0.0	0.0	2.291799255968979	0.0
46-47	0.0	0.0	0.0	2.6444594621193733	0.0
48-49	0.0	0.0	0.0	2.989574380138177	0.0
50-51	0.0	0.0	0.0	3.3274720660311132	0.0
52-53	0.0	0.0	0.0	3.646014447586492	0.0
54-55	0.0	0.0	0.0	3.976038789342117	0.0
56-57	0.0	0.0	0.0	4.370034052213394	0.0
58-59	0.0	0.0	0.0	4.742049562701345	0.0
60-61	0.0	0.0	0.0	5.096350048880344	0.0
62-63	0.0	0.0	0.0	5.458195823190935	0.0
64-65	0.0	0.0	0.0	5.842677461896295	0.0
66-67	0.0	0.0	0.0	6.264229429248161	0.0
68-69	0.0	0.0	0.0	6.765170949984581	0.0
70-71	0.0	0.0	0.0	7.289404447127213	0.0
72-73	0.0	0.0	0.0	7.8093732161954685	0.0
74-75	0.0	0.0	0.0	8.36411592187018	0.0
76-77	0.0	0.0	0.0	8.953960620157073	0.0
78-79	0.0	0.0	0.0	9.629755991942945	0.0
80-81	0.0	0.0	0.0	10.303911083700209	0.0
82-83	0.0	0.0	0.0	10.981674791520408	0.0
84-85	0.0	0.0	0.0	11.670264347529411	0.0
86-87	0.0	0.0	0.0	12.38870700005905	0.0
88	0.0	0.0	0.0	12.928687185476305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGA	65	2.7699098E-6	43.382492	9
GATAGCC	65	2.7699098E-6	43.382492	7
CTTAGGT	45	0.0010114294	41.775738	3
GCCTTAG	50	0.0016760168	37.697105	1
AGGTCCT	50	0.0016978066	37.598164	6
ATAGCCG	75	7.4037252E-6	37.59816	8
CCTTAGG	65	1.3919109E-4	36.223385	2
GGTCCTG	55	0.002710303	34.18015	7
CACAGGA	55	0.002710303	34.18015	7
GGATAGC	85	1.7454433E-5	33.174847	6
CTATAAT	60	0.0040976806	31.414253	1
ATGGATA	90	2.580165E-5	31.331804	4
ATGGGAG	105	2.0506523E-6	31.3318	5
TAGGTCC	60	0.004150762	31.3318	5
TTGTCCA	75	3.2632652E-4	31.3318	6
GTCCACG	65	0.0061391257	28.921663	8
GTTAGGT	85	6.712907E-4	27.700237	2
CTTAGAT	85	6.712907E-4	27.700237	2
CTGTGTG	85	6.790742E-4	27.645706	9
ACTGCTC	35	0.008828127	26.85583	92-93
>>END_MODULE