FastQCFastQC Report
Wed 25 May 2016
SRR1033058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415419
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC37400.036.4493644
GTATCAA39600.035.8535841
ATCAACG38600.035.3162233
CAACGCA38850.035.0884485
AACGCAG40550.033.7239466
TATCAAC46400.029.784682
GGTATCA27800.028.0726991
ACGCAGA49700.026.9486757
GTACATG82050.025.5550731
CGCAGAG51850.025.3115258
TACATGG83650.024.1638452
ACATGGG85500.022.3764843
GAGTACA62350.020.0570031
GCAGAGT65200.019.2673559
GTCTCGC8900.018.488381
AGTACAT63200.017.6276652
CATGGGG65300.016.7728274
GTATAAG14300.016.4382211
AGAGTAC55600.016.00202410-11
CCTATAC13500.015.6690383
Produced by FastQC (version 0.11.5)