##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033058_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3415419 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.417424333588354 31.0 31.0 34.0 30.0 34.0 2 31.564863930311333 33.0 31.0 34.0 30.0 34.0 3 31.66394196436806 34.0 31.0 34.0 30.0 34.0 4 35.126843587858474 37.0 35.0 37.0 33.0 37.0 5 35.18559714049726 37.0 35.0 37.0 33.0 37.0 6 35.33580184451747 37.0 35.0 37.0 33.0 37.0 7 35.28793275437069 37.0 35.0 37.0 33.0 37.0 8 35.291363080196014 37.0 35.0 37.0 33.0 37.0 9 36.95390433794507 39.0 38.0 39.0 34.0 39.0 10-11 36.936052209114024 39.0 38.0 39.0 34.0 39.0 12-13 36.86891271612649 39.0 38.0 39.0 33.5 39.0 14-15 38.24891104136857 41.0 39.0 41.0 34.0 41.0 16-17 38.24793956466249 41.0 38.5 41.0 34.0 41.0 18-19 38.25268861595019 40.0 38.5 41.0 34.0 41.0 20-21 38.269650810047025 40.0 39.0 41.0 34.0 41.0 22-23 38.22732920909557 40.0 38.5 41.0 34.0 41.0 24-25 38.22884776362724 40.0 38.0 41.0 34.0 41.0 26-27 38.13123894901328 40.0 38.0 41.0 33.0 41.0 28-29 38.14218270144893 40.0 38.0 41.0 33.0 41.0 30-31 38.29337864548977 40.0 39.0 41.0 34.0 41.0 32-33 38.28318399587283 40.0 38.5 41.0 34.0 41.0 34-35 38.24658438686439 40.0 38.0 41.0 33.5 41.0 36-37 38.17441827781599 40.0 38.0 41.0 33.0 41.0 38-39 38.09001252847747 40.0 38.0 41.0 33.0 41.0 40-41 38.02384773288431 40.0 38.0 41.0 33.0 41.0 42-43 37.912572513065015 40.0 38.0 41.0 33.0 41.0 44-45 37.781375872184356 40.0 38.0 41.0 33.0 41.0 46-47 37.65534609370036 40.0 38.0 41.0 32.0 41.0 48-49 37.54743078374864 40.0 38.0 41.0 32.0 41.0 50-51 37.428767890557495 40.0 37.0 41.0 32.0 41.0 52-53 37.28052107223155 40.0 37.0 41.0 31.0 41.0 54-55 37.12866547266968 40.0 37.0 41.0 31.0 41.0 56-57 36.96346861102547 40.0 36.0 41.0 31.0 41.0 58-59 36.77109133608497 40.0 36.0 41.0 31.0 41.0 60-61 36.55170946815018 39.0 35.5 41.0 30.5 41.0 62-63 36.30165596080598 39.0 35.0 41.0 30.0 41.0 64-65 36.02951189883291 39.0 35.0 40.5 30.0 41.0 66-67 35.732655641957834 38.0 35.0 40.0 29.5 41.0 68-69 35.41591222043327 37.5 35.0 40.0 29.0 41.0 70-71 35.075298521206335 37.0 34.5 40.0 29.0 41.0 72-73 34.7187225052036 36.5 34.0 39.0 29.0 41.0 74-75 34.35385438799749 36.0 34.0 39.0 29.0 40.5 76-77 33.980556265570925 35.5 34.0 38.5 28.0 40.0 78-79 33.58426433184333 35.0 34.0 37.0 28.0 39.0 80-81 33.21980860913405 35.0 33.5 37.0 27.0 39.0 82-83 32.98674642847627 35.0 34.0 36.5 27.5 39.0 84-85 32.75396561885965 35.0 33.5 36.0 27.0 37.0 86-87 32.571186873411435 35.0 34.0 36.0 27.0 37.0 88-89 32.34555804134134 35.0 33.0 35.5 27.0 37.0 90-91 32.12693098562724 35.0 33.0 35.0 27.0 36.0 92-93 31.90010830296371 35.0 33.0 35.0 26.0 36.0 94-95 31.696300366075143 35.0 33.0 35.0 26.0 36.0 96-97 31.520284626864232 35.0 33.0 35.0 25.0 36.0 98-99 31.342588713127142 35.0 33.0 35.0 25.0 35.0 100 31.199650174693062 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0885013900524072 1101 2 0.1697398375593977 1101 3 0.09134554050485022 1101 4 0.028735016423063087 1101 5 0.03250158999786379 1101 6 0.1869980100361488 1101 7 0.20346118535461954 1101 8 0.27511893085143413 1101 9 0.3291832988160337 1101 10-11 0.28269873908043763 1101 12-13 0.30490555663023855 1101 14-15 0.4482092553916388 1101 16-17 0.3820054968972073 1101 18-19 0.4131297730536687 1101 20-21 0.39518777559642615 1101 22-23 0.36094884738187005 1101 24-25 0.3588671001004613 1101 26-27 0.25721431717358456 1101 28-29 0.29172395989292 1101 30-31 0.38880668158492426 1101 32-33 0.3183048243223965 1101 34-35 0.3583825008119348 1101 36-37 0.3625556331087907 1101 38-39 0.3605916307456667 1101 40-41 0.352998599686309 1101 42-43 0.3508879054598779 1101 44-45 0.346505441757067 1101 46-47 0.30956687206594324 1101 48-49 0.34951462655219956 1101 50-51 0.3039706602077743 1101 52-53 0.23948511577525977 1101 54-55 0.2191188970010387 1101 56-57 0.2376979974527984 1101 58-59 0.2850144565230224 1101 60-61 0.24594552442076179 1101 62-63 0.2477382633658607 1101 64-65 0.23247561220879476 1101 66-67 0.25428847055989934 1101 68-69 0.1825492486676339 1101 70-71 0.12992371985440343 1101 72-73 0.09351185865013178 1101 74-75 0.05058217599731307 1101 76-77 0.08969607408297975 1101 78-79 0.07474560062654234 1101 80-81 0.20655307606170226 1101 82-83 0.13209790128281185 1101 84-85 0.1298441110280777 1101 86-87 0.11041955100705536 1101 88-89 0.1883966160982382 1101 90-91 0.19974956518566955 1101 92-93 0.2880742404426684 1101 94-95 0.32712854369956545 1101 96-97 0.33580280361647397 1101 98-99 0.3158547471593849 1101 100 0.25234857917315523 1102 1 -0.0476059294051403 1102 2 0.07248827941113944 1102 3 0.09227881568521212 1102 4 -0.060876776328818494 1102 5 -0.011685812229600856 1102 6 0.13522853838367155 1102 7 0.21921408969460288 1102 8 0.23693048152777862 1102 9 0.29749312008895856 1102 10-11 0.23939967391702766 1102 12-13 0.22233943214385476 1102 14-15 0.3175520392489162 1102 16-17 0.2832108571385703 1102 18-19 0.2476751026171513 1102 20-21 0.3266082351781634 1102 22-23 0.3113126033439073 1102 24-25 0.32180595960343794 1102 26-27 0.24098556388164383 1102 28-29 0.25058794621041613 1102 30-31 0.34596718482708866 1102 32-33 0.3403131239735657 1102 34-35 0.3418527954317554 1102 36-37 0.37958144291602736 1102 38-39 0.3844532230611648 1102 40-41 0.2935339278530975 1102 42-43 0.2923675767223344 1102 44-45 0.294749608632479 1102 46-47 0.2051405199737033 1102 48-49 0.24958027714139774 1102 50-51 0.2934388604447875 1102 52-53 0.31593675260330656 1102 54-55 0.35272453437348616 1102 56-57 0.27454465841270803 1102 58-59 0.2083099475828547 1102 60-61 0.22359219143589115 1102 62-63 0.2623211581479268 1102 64-65 0.2505934325235444 1102 66-67 0.2863586715142077 1102 68-69 0.31221889734010944 1102 70-71 0.15838280517522918 1102 72-73 0.2473455988543094 1102 74-75 0.23698048403914385 1102 76-77 0.22225342516382796 1102 78-79 0.15105828156315226 1102 80-81 0.24085242656310868 1102 82-83 0.15972022840507094 1102 84-85 0.19606782782349796 1102 86-87 0.21410451938804442 1102 88-89 0.2812042991451378 1102 90-91 0.278015578459744 1102 92-93 0.3218269073342199 1102 94-95 0.35536831768369126 1102 96-97 0.2661617430121872 1102 98-99 0.3214649901175015 1102 100 0.21138477273190404 1103 1 0.009285210186771309 1103 2 0.12455161867866238 1103 3 0.07951947789557678 1103 4 0.07968396278393897 1103 5 0.06451675877427476 1103 6 0.06298706478990113 1103 7 0.16106160097504585 1103 8 0.1260577689455502 1103 9 0.27195175522884796 1103 10-11 0.2499981913727325 1103 12-13 0.28863023913760344 1103 14-15 0.3351281548826961 1103 16-17 0.39247589840590535 1103 18-19 0.3513873450995675 1103 20-21 0.3715222648947716 1103 22-23 0.36402141353182316 1103 24-25 0.32689576819519317 1103 26-27 0.24672056725651004 1103 28-29 0.23668118893758106 1103 30-31 0.21127689443099484 1103 32-33 0.1856767926452605 1103 34-35 0.23737344355175338 1103 36-37 0.31319065121108025 1103 38-39 0.322099880727599 1103 40-41 0.3356883335177443 1103 42-43 0.2972127276732408 1103 44-45 0.34087003615559297 1103 46-47 0.34736220188729305 1103 48-49 0.328873859959252 1103 50-51 0.2578527714683858 1103 52-53 0.3420418977682189 1103 54-55 0.31133247473813697 1103 56-57 0.2823450536756269 1103 58-59 0.29465573167055226 1103 60-61 0.277248566741811 1103 62-63 0.23341957062542917 1103 64-65 0.21404666190775146 1103 66-67 0.2708025231839315 1103 68-69 0.2531697448100658 1103 70-71 0.3089357793073262 1103 72-73 0.284950803710899 1103 74-75 0.2644820554904541 1103 76-77 0.26651809502650536 1103 78-79 0.2568493316037461 1103 80-81 0.3312567845250385 1103 82-83 0.2529530606504693 1103 84-85 0.24312353235512063 1103 86-87 0.20693141600712295 1103 88-89 0.19991265591561813 1103 90-91 0.27899448516040337 1103 92-93 0.2688174296580286 1103 94-95 0.18387916633550816 1103 96-97 0.2154371403484383 1103 98-99 0.20889953292297037 1103 100 0.15799855174346433 1104 1 -0.16015845410782248 1104 2 -0.12050957681487873 1104 3 -0.11621039488177942 1104 4 -0.12019650330564957 1104 5 -0.12539588527920387 1104 6 -0.007193769410299922 1104 7 0.03370053598040812 1104 8 0.04529052803221845 1104 9 0.08133435088851826 1104 10-11 0.09499470000297094 1104 12-13 0.03724190946845596 1104 14-15 0.050408926094839046 1104 16-17 0.06455760417996714 1104 18-19 0.1368720498113163 1104 20-21 0.1421034648890256 1104 22-23 0.10326935152774297 1104 24-25 0.026867506301641697 1104 26-27 -0.11975142842809561 1104 28-29 -0.02277609886932197 1104 30-31 0.041194745203341654 1104 32-33 0.024821500820799258 1104 34-35 0.05907628330945869 1104 36-37 0.11529866635910224 1104 38-39 0.11484698752380496 1104 40-41 0.155093730634853 1104 42-43 0.14755338166801124 1104 44-45 0.14802086884452592 1104 46-47 0.1146246701588467 1104 48-49 0.10835120245875629 1104 50-51 0.023331806151531964 1104 52-53 0.052993039550926824 1104 54-55 0.11751682126734408 1104 56-57 0.08391940220147376 1104 58-59 0.05195342251280266 1104 60-61 0.11625591461351092 1104 62-63 0.13719702310584125 1104 64-65 0.05952322560567325 1104 66-67 0.053322464806903724 1104 68-69 0.1298882543647224 1104 70-71 0.07368986663680488 1104 72-73 0.09542178011409419 1104 74-75 0.13351205038301117 1104 76-77 0.036284862702046894 1104 78-79 0.1144917248853119 1104 80-81 0.0803889502443127 1104 82-83 0.06909338172411594 1104 84-85 0.05759335710269653 1104 86-87 0.07045649705874268 1104 88-89 0.023225629360247524 1104 90-91 0.06174319147829266 1104 92-93 0.02240453905759665 1104 94-95 0.1296325686284483 1104 96-97 0.0908718407028708 1104 98-99 0.11797153456441123 1104 100 0.17138401109851387 1105 1 -0.04164597726956032 1105 2 -4.952240499633831E-5 1105 3 0.0012405783543592008 1105 4 0.011783096638872337 1105 5 0.0166554026541732 1105 6 0.10890719647456137 1105 7 0.07966214041495334 1105 8 0.07929451336303117 1105 9 0.1450551816975647 1105 10-11 0.17769588256803814 1105 12-13 0.17562642194320688 1105 14-15 0.1963906135936 1105 16-17 0.18939182505404517 1105 18-19 0.2197180681822033 1105 20-21 0.17223475557522505 1105 22-23 0.15297070197304663 1105 24-25 0.1364696718524101 1105 26-27 0.1647154996868423 1105 28-29 0.14262343002327782 1105 30-31 0.19490065448336225 1105 32-33 0.11899699510296813 1105 34-35 0.10978679209085129 1105 36-37 0.17510728289040145 1105 38-39 0.15238719796845857 1105 40-41 0.20343656501631102 1105 42-43 0.21162438353763946 1105 44-45 0.19806079514405894 1105 46-47 0.1554273045229948 1105 48-49 0.17909847805449886 1105 50-51 0.1201469408154594 1105 52-53 0.17762922431094808 1105 54-55 0.1415616713586303 1105 56-57 0.0920058490527822 1105 58-59 0.10520219703921185 1105 60-61 0.16623723924070788 1105 62-63 0.15828738959569932 1105 64-65 0.0959573868353445 1105 66-67 0.09534463959629846 1105 68-69 0.18925687869567298 1105 70-71 0.1136236947128424 1105 72-73 0.10369908906690739 1105 74-75 0.11439364059466328 1105 76-77 0.15232557939424396 1105 78-79 0.15051801013364496 1105 80-81 0.18179426422727119 1105 82-83 0.2104749422178287 1105 84-85 0.19796573840564236 1105 86-87 0.14565512231773425 1105 88-89 0.18093868345626873 1105 90-91 0.18942572473891772 1105 92-93 0.16028157356271322 1105 94-95 0.14731302684764458 1105 96-97 0.1925208417111932 1105 98-99 0.17099278444840493 1105 100 0.20888762156631557 1106 1 -0.04850485031207796 1106 2 -0.01377245083516243 1106 3 -0.006206412340269196 1106 4 0.08821648422717487 1106 5 0.10105546807735521 1106 6 0.11656340472379867 1106 7 0.17638048317635935 1106 8 0.24355903219366581 1106 9 0.26108690770038123 1106 10-11 0.22649423806858948 1106 12-13 0.2909484022240747 1106 14-15 0.2906483435521139 1106 16-17 0.26552540219839216 1106 18-19 0.28773654434188956 1106 20-21 0.2541004971880483 1106 22-23 0.2320704650220904 1106 24-25 0.19570185481243385 1106 26-27 0.12458960246389239 1106 28-29 0.1604550158986271 1106 30-31 0.1766353416546309 1106 32-33 0.20048352253677137 1106 34-35 0.1845045682491886 1106 36-37 0.14467083991704754 1106 38-39 0.20679548133468728 1106 40-41 0.20202343372323384 1106 42-43 0.19590913785561526 1106 44-45 0.30100005248416295 1106 46-47 0.19809447460999507 1106 48-49 0.2384755507471752 1106 50-51 0.21300129563822878 1106 52-53 0.19867054668210216 1106 54-55 0.21879738019784156 1106 56-57 0.23750819771515097 1106 58-59 0.3417241736767309 1106 60-61 0.284204198178557 1106 62-63 0.26864523288268316 1106 64-65 0.29271206882898326 1106 66-67 0.310033572863162 1106 68-69 0.35254374026510504 1106 70-71 0.30804967814331263 1106 72-73 0.30448683300912194 1106 74-75 0.3774118682373384 1106 76-77 0.30099608801079114 1106 78-79 0.2769802068496574 1106 80-81 0.40529477425640437 1106 82-83 0.3662888032164915 1106 84-85 0.3453436052534471 1106 86-87 0.28993298490188124 1106 88-89 0.2695448108569991 1106 90-91 0.21448819359086002 1106 92-93 0.2962317412312103 1106 94-95 0.36421228920362836 1106 96-97 0.2583124209814045 1106 98-99 0.2579377739333495 1106 100 0.27642040991062444 1107 1 8.942665724518406E-4 1107 2 0.04701123371790672 1107 3 0.024162745763465665 1107 4 0.13266096533291005 1107 5 0.034966161726359246 1107 6 0.07739732440862923 1107 7 0.11276663873699277 1107 8 0.13908934278536123 1107 9 0.10985647692381661 1107 10-11 0.1216568588411846 1107 12-13 0.16310164164768537 1107 14-15 0.1506405979381995 1107 16-17 0.2033915969242841 1107 18-19 0.20660103239310956 1107 20-21 0.1316208118958926 1107 22-23 0.15687511305034718 1107 24-25 0.1443087991388765 1107 26-27 0.14188463446507882 1107 28-29 0.20611289402935284 1107 30-31 0.17295906212292778 1107 32-33 0.12917520668400329 1107 34-35 0.10735590277969465 1107 36-37 0.19217665325187738 1107 38-39 0.1991441098496054 1107 40-41 0.1855695720933923 1107 42-43 0.1645206355141937 1107 44-45 0.13022143268822362 1107 46-47 0.14900901796934818 1107 48-49 0.12993508342022153 1107 50-51 0.19377584053185615 1107 52-53 0.2554282030028574 1107 54-55 0.1993339474968252 1107 56-57 0.19289049201713482 1107 58-59 0.18539702171174355 1107 60-61 0.2230693213866104 1107 62-63 0.2218659317366729 1107 64-65 0.22671927332226716 1107 66-67 0.2098540791903929 1107 68-69 0.21318290601547574 1107 70-71 0.21405492107430746 1107 72-73 0.13125997230891073 1107 74-75 0.14516429565004074 1107 76-77 0.17623375627580629 1107 78-79 0.182140361234012 1107 80-81 0.2242492978516566 1107 82-83 0.2744082110523465 1107 84-85 0.2068874570589827 1107 86-87 0.218926162327584 1107 88-89 0.19999142509986 1107 90-91 0.24088370327947928 1107 92-93 0.23364878933568178 1107 94-95 0.273838750992347 1107 96-97 0.2867437320688495 1107 98-99 0.2986777234012621 1107 100 0.3261135961625321 1108 1 -0.043349250802140915 1108 2 0.05719429909814977 1108 3 0.06197108982636834 1108 4 0.03876082740790565 1108 5 0.13627863324379774 1108 6 0.073639733837922 1108 7 0.11709694757312405 1108 8 8.091846867515073E-4 1108 9 0.0866765139424217 1108 10-11 0.07925940430717304 1108 12-13 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2215 2 -0.12596011964260256 2215 3 -0.18171484380909675 2215 4 -0.1386569986725732 2215 5 -0.15321277195083383 2215 6 -0.17268214695388906 2215 7 -0.22007113318345972 2215 8 -0.20200537795018647 2215 9 -0.2824509622104969 2215 10-11 -0.24779376877920356 2215 12-13 -0.35716993648063067 2215 14-15 -0.40756674919625624 2215 16-17 -0.3825123816738696 2215 18-19 -0.360574609098677 2215 20-21 -0.30895272636043813 2215 22-23 -0.3651052623823716 2215 24-25 -0.2913154228754493 2215 26-27 -0.2827113253033815 2215 28-29 -0.33970202820545836 2215 30-31 -0.39521673244320965 2215 32-33 -0.36736211473830593 2215 34-35 -0.2935316335211269 2215 36-37 -0.24458958730090075 2215 38-39 -0.3543608271865324 2215 40-41 -0.27758642371740194 2215 42-43 -0.31269396697219065 2215 44-45 -0.28795274730052256 2215 46-47 -0.23403253224850573 2215 48-49 -0.21519429546116697 2215 50-51 -0.2780923528212398 2215 52-53 -0.354455711987157 2215 54-55 -0.30894931582491125 2215 56-57 -0.3588962707458663 2215 58-59 -0.35874970052535815 2215 60-61 -0.4170571341782008 2215 62-63 -0.49409806256635136 2215 64-65 -0.5833607507225196 2215 66-67 -0.4699569345251291 2215 68-69 -0.5261220246244562 2215 70-71 -0.587710603785986 2215 72-73 -0.6173739710138335 2215 74-75 -0.5246352204114331 2215 76-77 -0.4609372153613478 2215 78-79 -0.5047155038891589 2215 80-81 -0.5632086959676172 2215 82-83 -0.5364401965862129 2215 84-85 -0.4887491475220074 2215 86-87 -0.3946781426325572 2215 88-89 -0.40708483294314846 2215 90-91 -0.4976274296974239 2215 92-93 -0.4876587420158387 2215 94-95 -0.5954947887170938 2215 96-97 -0.5286229151711375 2215 98-99 -0.4717885665513819 2215 100 -0.4441089702268748 2216 1 -0.20598510144610316 2216 2 -0.30823407394990454 2216 3 -0.2215579470181872 2216 4 -0.3993748236178405 2216 5 -0.27588682948363896 2216 6 -0.4563135464002741 2216 7 -0.4093330957309007 2216 8 -0.39991078540499814 2216 9 -0.46419889409559545 2216 10-11 -0.4566726284855065 2216 12-13 -0.5593284980064652 2216 14-15 -0.6176761602689211 2216 16-17 -0.6082023044088913 2216 18-19 -0.6436482378180415 2216 20-21 -0.5438906791839955 2216 22-23 -0.5554769984850907 2216 24-25 -0.598270017081397 2216 26-27 -0.6212935210927171 2216 28-29 -0.5814256155799313 2216 30-31 -0.8274851674680761 2216 32-33 -0.8022916643300491 2216 34-35 -0.8952372161012789 2216 36-37 -0.9371745059362411 2216 38-39 -0.9242906825763626 2216 40-41 -0.8411779799114001 2216 42-43 -0.8702667208107044 2216 44-45 -0.7817734879683371 2216 46-47 -0.7517258848943627 2216 48-49 -0.8131804286369757 2216 50-51 -0.7499088083380627 2216 52-53 -0.7418667259887286 2216 54-55 -0.7893320457415598 2216 56-57 -0.8501075841809467 2216 58-59 -0.8057695117659023 2216 60-61 -0.7746826444010608 2216 62-63 -0.8734680041564289 2216 64-65 -0.791211109550467 2216 66-67 -0.7848536021216219 2216 68-69 -0.7817773996348336 2216 70-71 -0.7565048046610201 2216 72-73 -0.8122438228534179 2216 74-75 -0.8672338626679519 2216 76-77 -0.7483145915740437 2216 78-79 -0.7537990046655878 2216 80-81 -0.6651815162364088 2216 82-83 -0.8492561941517636 2216 84-85 -0.6555156585051307 2216 86-87 -0.6378196883598086 2216 88-89 -0.7500585377888278 2216 90-91 -0.775532175832808 2216 92-93 -0.863318656760331 2216 94-95 -0.706723520211959 2216 96-97 -0.7345188016151312 2216 98-99 -0.6417430846807299 2216 100 -0.6045987145548146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32525.0 3 6653.0 4 3677.0 5 3057.0 6 2612.0 7 3076.0 8 3521.0 9 4182.0 10 4525.0 11 5063.0 12 5726.0 13 6583.0 14 7192.0 15 7419.0 16 7344.0 17 7402.0 18 7100.0 19 7243.0 20 7364.0 21 7631.0 22 8979.0 23 12187.0 24 18367.0 25 24256.0 26 24611.0 27 23156.0 28 26010.0 29 32469.0 30 39783.0 31 48441.0 32 63275.0 33 85388.0 34 122160.0 35 186149.0 36 332531.0 37 694819.0 38 1107745.0 39 424484.0 40 714.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5981231418624 20.074653626158828 13.482652304295703 23.84457092768307 2 15.244352340712208 20.25137484347654 40.82755691595697 23.67671589985428 3 17.9502593199326 25.654568975821025 30.17403613370737 26.221135570539005 4 11.92953340670185 16.956624673152632 39.796970794407585 31.316871125737933 5 12.77567908213397 38.1565139517594 36.127612026244 12.940194939862625 6 30.617305006438194 38.665022098518186 18.071084494816915 12.6465884002267 7 26.87109617455144 32.60384754471835 23.113635790851955 17.41142048987826 8 24.603598417793492 36.954768308037885 20.724607437871196 17.71702583629743 9 24.66995379382421 17.078202325958987 21.37089849119222 36.88094538902458 10-11 23.481021654994336 27.185187790434405 29.44267368911594 19.891116865455324 12-13 24.14644415665 24.55401834501519 29.247459860661827 22.05207763767298 14-15 21.47516365166373 26.560290948015293 26.213386205615986 25.751159194704993 16-17 21.24076381834159 29.89809543539163 26.810543597473163 22.050597148793617 18-19 21.199071234878076 28.98397814222064 28.60227296872928 21.214677654172007 20-21 22.46045401142282 28.271810187460456 28.0073985440289 21.26033725708783 22-23 21.8970252262606 28.062026904163822 28.086166445949033 21.954781423626546 24-25 21.866710008719657 28.185556700117655 28.07787512656685 21.86985816459584 26-27 21.6305249614399 28.096624852956857 28.12029548100725 22.15255470459599 28-29 21.663983246434075 28.106769997511165 28.208842996405032 22.020403759649728 30-31 21.940728475317304 28.637750599913183 27.751275314548774 21.670245610220743 32-33 21.5399770056478 28.20989202499142 28.344391991589834 21.905738977770955 34-35 21.7131124664941 28.03436146750356 28.336379479643437 21.916146586358906 36-37 22.032935066700336 28.334870310924515 27.76023208166331 21.87196254071184 38-39 21.881569457196644 28.482243593659827 27.923188234447828 21.712998714695704 40-41 21.66629989477407 28.19958173352904 28.235360307232753 21.89875806446414 42-43 21.585712498355484 28.036231813990447 28.234467485745814 22.143588201908255 44-45 21.753800368628028 28.20673935015575 28.12015673943073 21.91930354178549 46-47 21.76627070993108 28.341363865520737 28.007391205420802 21.884974219127383 48-49 21.743917991873317 28.18198666357051 28.043626518441794 22.03046882611438 50-51 21.70973377429598 28.133724486245555 28.048271492350906 22.108270247107555 52-53 21.82908475000392 28.038891909341874 28.083312346434873 22.048710994219338 54-55 21.871067139328982 28.498584225093047 27.876680431572197 21.753668204005773 56-57 21.692490519501714 28.236268403212485 27.920510603537764 22.150730473748037 58-59 21.583497464602214 27.807677461364754 28.356814664362844 22.25201040967018 60-61 21.781408793032327 28.273333786469664 27.959512464374107 21.985744956123902 62-63 21.708195375941187 28.26992530560361 27.934960045156217 22.08691927329898 64-65 21.64134763594153 28.010721389485514 28.101750949335475 22.246180025237482 66-67 21.522660258438634 28.106724889192037 27.98481268707268 22.385802165296656 68-69 21.96848928767625 28.16549349935909 27.944698773118475 21.921318439846182 70-71 21.8462446200734 27.9979568656667 28.012136346844173 22.14366216741573 72-73 21.68424410894826 28.18491198779171 28.079709534591203 22.05113436866883 74-75 21.72313628293695 28.149525098583794 27.953968839904608 22.17336977857465 76-77 21.711849029320614 28.147197467026135 27.824951069814098 22.316002433839152 78-79 21.880608006805627 28.192114823856958 27.936127530505505 21.99114963883191 80-81 21.823374875707547 27.930398276302213 28.122543535130042 22.123683312860198 82-83 21.660531690152705 27.99416807389567 28.25956055200581 22.085739683945814 84-85 21.615240027920677 28.002139452840208 28.023229349403056 22.35939116983606 86-87 21.78694277856367 28.119582551570705 27.951716250591495 22.141758419274133 88-89 21.58816899164134 27.932239075977815 28.03850912610166 22.44108280627919 90-91 21.57152290791886 27.859749820632 28.12191488137108 22.446812390078062 92-93 21.738576449912124 28.01236086701816 28.031033977738723 22.21802870533099 94-95 21.502450403548064 27.804973565694148 28.131933247794137 22.56064278296365 96-97 21.552682685350902 27.763589177282487 28.183134979334667 22.500593158031947 98-99 21.45306573424689 27.931438205953107 28.075261437535172 22.540234622264833 100 21.641271621234466 27.80102751945274 27.939705735020908 22.61799512429189 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 311.0 1 277.0 2 280.5 3 314.5 4 334.0 5 345.5 6 315.0 7 297.0 8 272.0 9 235.0 10 203.0 11 203.0 12 228.5 13 239.0 14 273.0 15 323.5 16 405.0 17 563.5 18 775.0 19 1083.5 20 1588.0 21 2428.5 22 3737.5 23 5779.0 24 8570.0 25 12245.5 26 17366.0 27 24179.5 28 32582.0 29 41787.5 30 51498.0 31 62172.0 32 73364.5 33 85685.0 34 97492.0 35 106883.5 36 114217.5 37 120595.5 38 125910.0 39 128803.0 40 130470.5 41 130866.5 42 130435.0 43 129748.5 44 127702.5 45 126182.5 46 122750.0 47 116619.0 48 113320.5 49 111445.5 50 107707.5 51 102838.0 52 95880.0 53 90997.5 54 88052.0 55 82625.0 56 74726.0 57 67539.0 58 60480.0 59 51976.0 60 44758.0 61 38294.0 62 32082.0 63 25769.0 64 20433.0 65 16411.0 66 12862.5 67 9624.0 68 7333.5 69 5975.0 70 4809.0 71 3395.5 72 2384.0 73 1740.5 74 1207.0 75 733.5 76 413.5 77 278.5 78 183.5 79 93.5 80 59.0 81 42.5 82 32.0 83 20.5 84 13.0 85 12.5 86 11.0 87 9.0 88 7.0 89 5.0 90 7.5 91 7.0 92 4.5 93 4.5 94 4.0 95 2.5 96 1.5 97 1.0 98 1.0 99 1.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06016831317036064 2 0.04081490440850742 3 0.054898095958358255 4 0.07351953010743338 5 0.08730993181217298 6 0.08704642095157285 7 0.0896229715885518 8 0.1017444711761573 9 0.09723550756144414 10-11 0.1025935617269799 12-13 0.10679509600432625 14-15 0.10761490757063776 16-17 0.0766523814501237 18-19 0.09842130643414468 20-21 0.09152610558177489 22-23 0.05658163756774791 24-25 0.021124787324776257 26-27 0.17949481454544816 28-29 0.06369643080395114 30-31 0.07710620571004612 32-33 0.07047451571827644 34-35 0.02113942681703182 36-37 0.05249721922844606 38-39 0.07637423109726801 40-41 0.0808685552197256 42-43 0.07501275831750073 44-45 0.0730071478784887 46-47 0.0794778034554472 48-49 0.07573009343802327 50-51 0.07119185083879899 52-53 0.0754080246084009 54-55 0.06647793433250797 56-57 0.06909840344625359 58-59 0.06577523870424097 60-61 0.06356467537365108 62-63 0.06964006465970939 64-65 0.06564348327394091 66-67 0.067546617267164 68-69 0.06716599046851938 70-71 0.05961201246464929 72-73 0.058572608514504375 74-75 0.06087100879862764 76-77 0.05510304884993613 78-79 0.051911639538223565 80-81 0.04671461978749899 82-83 0.05257041668972386 84-85 0.04280587535526388 86-87 0.040770985931740736 88-89 0.04318650215390849 90-91 0.03948271061325126 92-93 0.04154687902128553 94-95 0.04068314897820736 96-97 0.043596407937064235 98-99 0.04409415067375335 100 0.051560291724090074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3415419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.23736012594633 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33424021709227 46.886042382057205 2 14.466704409707326 18.007389843651413 3 4.804421733816863 8.970435769334507 4 2.0444243122396415 5.0895828868439486 5 1.0605299900730312 3.300229345827076 6 0.6320825756216791 2.3603490532981284 7 0.38034262978657457 1.6570064854893567 8 0.2715358078216152 1.3519737486786896 9 0.18458666503812438 1.0339368071782655 >10 0.7698196474448871 8.146569178779227 >50 0.03701666471227817 1.556110524383492 >100 0.013824495763907789 1.4462901668231662 >500 4.7085088165045857E-4 0.19408380765554561 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.9278984511124405E-5 0.0 9 0.0 0.0 0.0 2.9278984511124405E-5 0.0 10-11 0.0 0.0 0.0 2.9278984511124405E-5 0.0 12-13 0.0 0.0 0.0 2.9278984511124405E-5 0.0 14-15 0.0 0.0 0.0 5.855796902224881E-5 0.0 16-17 0.0 0.0 0.0 8.783695353337321E-5 0.0 18-19 0.0 0.0 0.0 8.783695353337321E-5 0.0 20-21 0.0 0.0 0.0 8.783695353337321E-5 0.0 22-23 0.0 0.0 0.0 1.317554303000598E-4 0.0 24-25 0.0 0.0 0.0 2.0495289157787083E-4 0.0 26-27 0.0 0.0 0.0 2.92789845111244E-4 0.0 28-29 0.0 0.0 0.0 7.319746127781102E-4 0.0 30-31 0.0 0.0 0.0 0.0025619111447233852 0.0 32-33 0.0 0.0 0.0 0.007656454449659031 0.0 34-35 0.0 0.0 0.0 0.014888363623906758 0.0 36-37 0.0 0.0 0.0 0.02891299720473535 0.0 38-39 0.0 0.0 0.0 0.051911639538223565 0.0 40-41 0.0 0.0 0.0 0.08059040486686991 0.0 42-43 0.0 0.0 0.0 0.11105518825069487 0.0 44-45 0.0 0.0 0.0 0.1465559569704332 0.0 46-47 0.0 0.0 0.0 0.19006452795396406 0.0 48-49 0.0 0.0 0.0 0.23992663857640892 0.0 50-51 0.0 0.0 0.0 0.29697673989633483 0.0 52-53 0.0 0.0 0.0 0.35329486660348264 0.0 54-55 0.0 0.0 0.0 0.41640571771721124 0.0 56-57 0.0 0.0 0.0 0.4926189143996681 0.0 58-59 0.0 0.0 0.0 0.5799142067195855 0.0 60-61 0.0 0.0 0.0 0.6761395893153959 0.0 62-63 0.0 0.0 0.0 0.772438169372484 0.0 64-65 0.0 0.0 0.0 0.8797602870980106 0.0 66-67 0.0 0.0 0.0 1.0003311453148207 0.0 68-69 0.0 0.0 0.0 1.1375031877494388 0.0 70-71 0.0 0.0 0.0 1.2929453165189981 0.0 72-73 0.0 0.0 0.0 1.4510664723713256 0.0 74-75 0.0 0.0 0.0 1.6168880011500786 0.0 76-77 0.0 0.0 0.0 1.7971440692928158 0.0 78-79 0.0 0.0 0.0 2.007206143667878 0.0 80-81 0.0 0.0 0.0 2.2382466104451604 0.0 82-83 0.0 0.0 0.0 2.481101147472682 0.0 84-85 0.0 0.0 0.0 2.7237214526241145 0.0 86-87 0.0 0.0 0.0 2.985519492630333 0.0 88 0.0 0.0 0.0 3.2030916265324985 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 3740 0.0 36.449364 4 GTATCAA 3960 0.0 35.853584 1 ATCAACG 3860 0.0 35.316223 3 CAACGCA 3885 0.0 35.088448 5 AACGCAG 4055 0.0 33.723946 6 TATCAAC 4640 0.0 29.78468 2 GGTATCA 2780 0.0 28.072699 1 ACGCAGA 4970 0.0 26.948675 7 GTACATG 8205 0.0 25.555073 1 CGCAGAG 5185 0.0 25.311525 8 TACATGG 8365 0.0 24.163845 2 ACATGGG 8550 0.0 22.376484 3 GAGTACA 6235 0.0 20.057003 1 GCAGAGT 6520 0.0 19.267355 9 GTCTCGC 890 0.0 18.48838 1 AGTACAT 6320 0.0 17.627665 2 CATGGGG 6530 0.0 16.772827 4 GTATAAG 1430 0.0 16.438221 1 AGAGTAC 5560 0.0 16.002024 10-11 CCTATAC 1350 0.0 15.669038 3 >>END_MODULE