FastQCFastQC Report
Wed 25 May 2016
SRR1033058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415419
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.12953022747721435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.1145686663920298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45500.044.230381
GTATCAA76900.043.2082821
TCAACGC90000.036.136974
ATCAACG90500.035.936793
CAACGCA91350.035.6548965
AACGCAG93850.034.805276
TATCAAC97600.033.593842
ACGCAGA110000.029.4388627
CGCAGAG112500.028.659338
GTACATG86850.025.0712721
GCAGAGT128000.024.6744469
TACATGG85800.024.2131652
ACATGGG87150.022.9733313
GTGGTAT23250.021.284851
AGAGTAC118350.020.84855710-11
GAGTACT88750.020.59919712-13
CAGAGTA128550.019.41365810-11
CATGGGG64900.019.1182164
GAGTACA62900.019.1070561
AGTACTT94900.018.74427812-13
Produced by FastQC (version 0.11.5)