##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033058_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3415419 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64979523742182 34.0 31.0 34.0 31.0 34.0 2 32.64630430409856 34.0 31.0 34.0 31.0 34.0 3 32.754762739212964 34.0 31.0 34.0 31.0 34.0 4 36.267874600451655 37.0 37.0 37.0 35.0 37.0 5 36.25125497047361 37.0 37.0 37.0 35.0 37.0 6 36.31529513655572 37.0 37.0 37.0 35.0 37.0 7 36.259680583846375 37.0 37.0 37.0 35.0 37.0 8 36.26390202783319 37.0 37.0 37.0 35.0 37.0 9 38.060745987534766 39.0 39.0 39.0 35.0 39.0 10-11 38.01637924951521 39.0 38.0 39.0 35.0 39.0 12-13 37.985583028026724 39.0 38.0 39.0 35.0 39.0 14-15 39.44924239163628 41.0 39.0 41.0 36.0 41.0 16-17 39.450997959547564 41.0 39.0 41.0 36.0 41.0 18-19 39.47026894796802 41.0 39.0 41.0 36.5 41.0 20-21 39.4668658515983 41.0 39.0 41.0 36.0 41.0 22-23 39.394433011001 41.0 39.0 41.0 36.0 41.0 24-25 39.34370014337919 41.0 39.0 41.0 36.0 41.0 26-27 39.23176497524901 41.0 39.0 41.0 36.0 41.0 28-29 39.1844713635428 40.5 39.0 41.0 36.0 41.0 30-31 39.19000626277479 41.0 39.0 41.0 36.0 41.0 32-33 39.16123190156171 41.0 39.0 41.0 35.0 41.0 34-35 39.08777898114404 41.0 39.0 41.0 35.0 41.0 36-37 39.0051955558015 41.0 39.0 41.0 35.0 41.0 38-39 38.916199008086565 41.0 39.0 41.0 35.0 41.0 40-41 38.82084906712764 41.0 39.0 41.0 35.0 41.0 42-43 38.70864789942317 41.0 38.5 41.0 35.0 41.0 44-45 38.553268720470314 40.5 38.0 41.0 35.0 41.0 46-47 38.37851241677815 40.0 38.0 41.0 34.5 41.0 48-49 38.19173489402033 40.0 38.0 41.0 34.0 41.0 50-51 38.06406505321895 40.0 38.0 41.0 34.0 41.0 52-53 37.93677905990451 40.0 38.0 41.0 33.5 41.0 54-55 37.796134383511955 40.0 37.5 41.0 33.0 41.0 56-57 37.65191181521213 40.0 37.0 41.0 33.0 41.0 58-59 37.48227011092929 40.0 37.0 41.0 33.0 41.0 60-61 37.29603820204783 40.0 36.5 41.0 33.0 41.0 62-63 37.06999595071645 39.0 36.0 41.0 32.5 41.0 64-65 36.810092846587786 39.0 35.5 41.0 32.0 41.0 66-67 36.53477011166126 39.0 35.0 41.0 32.0 41.0 68-69 36.22422797905615 38.0 35.0 40.5 31.0 41.0 70-71 35.89407946726302 37.0 35.0 40.0 31.0 41.0 72-73 35.54280148350759 37.0 35.0 39.5 31.0 41.0 74-75 35.18791442572639 36.0 35.0 39.0 31.0 41.0 76-77 34.82427807539865 36.0 35.0 39.0 30.5 40.5 78-79 34.45250377772098 35.5 34.0 37.5 30.0 39.5 80-81 34.11168629676183 35.0 34.0 37.0 30.0 39.0 82-83 33.802615579523334 35.0 34.0 37.0 30.0 39.0 84-85 33.570519312564585 35.0 34.0 36.0 30.0 37.5 86-87 33.35465267951019 35.0 34.0 36.0 30.0 37.0 88-89 33.138333100565404 35.0 34.0 35.5 30.0 37.0 90-91 32.94845098068495 35.0 34.0 35.0 29.0 36.0 92-93 32.76752398461214 35.0 34.0 35.0 29.0 36.0 94-95 32.60850528148963 35.0 34.0 35.0 29.0 36.0 96-97 32.45735779416815 35.0 34.0 35.0 29.0 36.0 98-99 32.32759333481485 35.0 34.0 35.0 29.0 35.5 100 32.21992821378578 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11603653250544 1101 2 0.05613058197543097 1101 3 0.09403861710431727 1101 4 0.09110600789424694 1101 5 0.08529592699401434 1101 6 0.10320590368889526 1101 7 0.17446492399076163 1101 8 0.18854692127237627 1101 9 0.22464657743397964 1101 10-11 0.23873982674473382 1101 12-13 0.2590591547780363 1101 14-15 0.3187397642476526 1101 16-17 0.2785656284296678 1101 18-19 0.308192287349371 1101 20-21 0.3058040571549441 1101 22-23 0.31074586353243916 1101 24-25 0.34192831239909083 1101 26-27 0.30180767664968755 1101 28-29 0.3142749807092855 1101 30-31 0.36026232135154146 1101 32-33 0.2910745347441974 1101 34-35 0.2748682852506761 1101 36-37 0.30122318987237406 1101 38-39 0.271324600859721 1101 40-41 0.31423472293676014 1101 42-43 0.24308763125456068 1101 44-45 0.2629291823389224 1101 46-47 0.2927388528370187 1101 48-49 0.23821204099906623 1101 50-51 0.282578898420077 1101 52-53 0.27863477026822636 1101 54-55 0.31441168391457097 1101 56-57 0.29898025618435753 1101 58-59 0.2745511113541923 1101 60-61 0.2834377902016598 1101 62-63 0.26075716181606623 1101 64-65 0.3174245256419468 1101 66-67 0.34061841226182565 1101 68-69 0.3137963647727986 1101 70-71 0.27402232022046036 1101 72-73 0.29017559472787724 1101 74-75 0.21286773322661645 1101 76-77 0.25573602355134994 1101 78-79 0.18337051820270744 1101 80-81 0.19399280239490224 1101 82-83 0.1938390856014962 1101 84-85 0.265786300035451 1101 86-87 0.27088231827765696 1101 88-89 0.28899739790026757 1101 90-91 0.2922001814123547 1101 92-93 0.3316396497786016 1101 94-95 0.36929419995461643 1101 96-97 0.47278298616463843 1101 98-99 0.4863836311991818 1101 100 0.46193458753266725 1102 1 0.10517526589607229 1102 2 0.07070228983235438 1102 3 0.13733458801183218 1102 4 0.11237782703965848 1102 5 0.09248902465167674 1102 6 0.12141945552048838 1102 7 0.14548645591781906 1102 8 0.12549803002994508 1102 9 0.1357807383712739 1102 10-11 0.168507668057579 1102 12-13 0.1856393888808654 1102 14-15 0.2826548376819602 1102 16-17 0.22835404321191533 1102 18-19 0.193214451266293 1102 20-21 0.2025369561309205 1102 22-23 0.22465885667470786 1102 24-25 0.28431762348729706 1102 26-27 0.23245907083094153 1102 28-29 0.2952130077146222 1102 30-31 0.23736376143726545 1102 32-33 0.230554626444615 1102 34-35 0.22632569405605096 1102 36-37 0.21037448022944716 1102 38-39 0.2596475931898681 1102 40-41 0.2433499142765001 1102 42-43 0.24187099364058184 1102 44-45 0.20817065949668745 1102 46-47 0.2211502913775263 1102 48-49 0.19274571140635288 1102 50-51 0.23214890634042007 1102 52-53 0.23338729977480455 1102 54-55 0.209305095367867 1102 56-57 0.21193507728732897 1102 58-59 0.23999874592885106 1102 60-61 0.2857359892590452 1102 62-63 0.2637597801277991 1102 64-65 0.23567373758256593 1102 66-67 0.23029757410503038 1102 68-69 0.2063744635534448 1102 70-71 0.17127905291665257 1102 72-73 0.1822509007283628 1102 74-75 0.19009810381469094 1102 76-77 0.11341193937730765 1102 78-79 0.1460182796323366 1102 80-81 0.19813439249777787 1102 82-83 0.17089096522865788 1102 84-85 0.17862484852286542 1102 86-87 0.14611294745031955 1102 88-89 0.1654109372977146 1102 90-91 0.11083061043902376 1102 92-93 0.22563313260923934 1102 94-95 0.20593536424028258 1102 96-97 0.20194783793120763 1102 98-99 0.30285167520007406 1102 100 0.3239651924844509 1103 1 0.1510423604650839 1103 2 0.11846764419335898 1103 3 0.13570675867462256 1103 4 0.0887664317372483 1103 5 0.1269068227678929 1103 6 0.1427127703010811 1103 7 0.15363439297434667 1103 8 0.14068756008201433 1103 9 0.19805747561770914 1103 10-11 0.22929710683706617 1103 12-13 0.1982648691181268 1103 14-15 0.26835239868091776 1103 16-17 0.26515909424162487 1103 18-19 0.282748148346073 1103 20-21 0.31383590963501007 1103 22-23 0.29579463052783694 1103 24-25 0.28986354185135355 1103 26-27 0.29898901271968725 1103 28-29 0.3266228192960199 1103 30-31 0.3208968110671435 1103 32-33 0.2837969108695262 1103 34-35 0.29051518830231515 1103 36-37 0.24656873858036477 1103 38-39 0.24398729507756656 1103 40-41 0.20130044078287312 1103 42-43 0.23285458178880702 1103 44-45 0.2539238934555783 1103 46-47 0.2820741860068523 1103 48-49 0.25722906175434446 1103 50-51 0.2482463297861912 1103 52-53 0.2314341576960004 1103 54-55 0.2650327304793123 1103 56-57 0.2693739351982245 1103 58-59 0.29007435692172834 1103 60-61 0.22729684701879194 1103 62-63 0.18238888549543475 1103 64-65 0.24737494028013174 1103 66-67 0.26664580200776555 1103 68-69 0.2932747313237769 1103 70-71 0.3097227164448313 1103 72-73 0.3234158282363353 1103 74-75 0.35536710157715845 1103 76-77 0.321135642109617 1103 78-79 0.3052080688852712 1103 80-81 0.28056947423119993 1103 82-83 0.24080987106961516 1103 84-85 0.2168837854071839 1103 86-87 0.24733270569948473 1103 88-89 0.2521906481285825 1103 90-91 0.2358715132754199 1103 92-93 0.30368253283443636 1103 94-95 0.2769589573184774 1103 96-97 0.3024018674692499 1103 98-99 0.3526438542739996 1103 100 0.35390708123222936 1104 1 0.03638189479467968 1104 2 0.039250549388050615 1104 3 0.07138833692818736 1104 4 0.0176200600591514 1104 5 0.044401508494971154 1104 6 0.06263211028327476 1104 7 0.07329467663021205 1104 8 0.09102878769836309 1104 9 0.06046366367071698 1104 10-11 0.09297113093579412 1104 12-13 0.1145734157486018 1104 14-15 0.14216725790646478 1104 16-17 0.13944297735078948 1104 18-19 0.14549910502550745 1104 20-21 0.1454427891226331 1104 22-23 0.16609305715005718 1104 24-25 0.1352620488909011 1104 26-27 0.1280811573032139 1104 28-29 0.15981154851464652 1104 30-31 0.15518786444594213 1104 32-33 0.1486140965717837 1104 34-35 0.09853794784125824 1104 36-37 0.14841835927310854 1104 38-39 0.1001241964841526 1104 40-41 0.0821815014532774 1104 42-43 0.05080869426937795 1104 44-45 0.08366707645960503 1104 46-47 0.09363149135333515 1104 48-49 -0.009020964444722779 1104 50-51 0.01825066504152062 1104 52-53 0.060490539180818814 1104 54-55 0.037864645018522936 1104 56-57 0.07471920574352708 1104 58-59 0.07973405592483829 1104 60-61 0.05695034649646402 1104 62-63 0.005846566012486676 1104 64-65 0.09102555620629715 1104 66-67 0.08260377760898763 1104 68-69 0.09694132040444003 1104 70-71 0.0671623124387466 1104 72-73 0.03619757295047066 1104 74-75 -0.017161830460821648 1104 76-77 0.020356311018559836 1104 78-79 0.021038854934353424 1104 80-81 0.02548845569990732 1104 82-83 0.024226471457254206 1104 84-85 0.02942465318630383 1104 86-87 0.00866103675856067 1104 88-89 0.01714634137783122 1104 90-91 0.06821251806184847 1104 92-93 0.01346064056718177 1104 94-95 0.024734066775401686 1104 96-97 0.039397105307095615 1104 98-99 0.033507441548863426 1104 100 0.08397912589529 1105 1 0.020532582425872192 1105 2 0.0315592571586123 1105 3 0.07549045399180443 1105 4 0.005354750893623361 1105 5 -0.015262084538314014 1105 6 0.05389670726122375 1105 7 0.0638561369588615 1105 8 0.06725073162636619 1105 9 0.07081526327739596 1105 10-11 0.08442041702770098 1105 12-13 0.09725632960726927 1105 14-15 0.17121675337942577 1105 16-17 0.13645199804593489 1105 18-19 0.15124903438025683 1105 20-21 0.10456478591726892 1105 22-23 0.18800705867453615 1105 24-25 0.11448400220351829 1105 26-27 0.1424757092246054 1105 28-29 0.18226041021365802 1105 30-31 0.19053163833108044 1105 32-33 0.17640934941923092 1105 34-35 0.13953075610221788 1105 36-37 0.15222669635159036 1105 38-39 0.1508254249295291 1105 40-41 0.13621614317496267 1105 42-43 0.07784819409054933 1105 44-45 0.034593660659894 1105 46-47 0.11686207016475691 1105 48-49 0.11745852444860105 1105 50-51 0.17871789346395417 1105 52-53 0.16859077098067843 1105 54-55 0.10309214359258334 1105 56-57 0.12302773416377732 1105 58-59 0.12249377759818003 1105 60-61 0.10861925330416966 1105 62-63 0.15527367731499453 1105 64-65 0.12525383370294918 1105 66-67 0.04942284338154934 1105 68-69 0.1246480451027594 1105 70-71 0.08524301382360022 1105 72-73 0.09307326962738216 1105 74-75 0.13378867406620998 1105 76-77 0.13503369421405864 1105 78-79 0.13890247987531268 1105 80-81 0.17643583202379176 1105 82-83 0.22052778832650688 1105 84-85 0.2266107093250369 1105 86-87 0.19966105031409853 1105 88-89 0.15044710664059124 1105 90-91 0.1576810050646955 1105 92-93 0.16936348691923087 1105 94-95 0.08703777984826644 1105 96-97 0.1988230383197518 1105 98-99 0.0943256752193875 1105 100 0.10383562478946828 1106 1 0.03216682946684557 1106 2 0.007942800009189455 1106 3 0.017308657795695126 1106 4 0.014348553829606203 1106 5 0.06060297253383595 1106 6 0.08235418144744955 1106 7 0.08767136845225565 1106 8 0.05842932018639857 1106 9 0.08875430429385744 1106 10-11 0.1166277105188982 1106 12-13 0.11795198563209652 1106 14-15 0.16891329911869235 1106 16-17 0.18436169913690748 1106 18-19 0.15550662535770243 1106 20-21 0.15821744802907034 1106 22-23 0.1619266516060307 1106 24-25 0.15680719413237654 1106 26-27 0.118131858897641 1106 28-29 0.13304043952845745 1106 30-31 0.12696652408824605 1106 32-33 0.08546855590639524 1106 34-35 0.11257812501381181 1106 36-37 0.1267362914243435 1106 38-39 0.09606368206917182 1106 40-41 0.13754798624381692 1106 42-43 0.13586613788204716 1106 44-45 0.1978215141631594 1106 46-47 0.1446273086739538 1106 48-49 0.2408136893268633 1106 50-51 0.18860205862780788 1106 52-53 0.17801932163499146 1106 54-55 0.22894084094339462 1106 56-57 0.26028471652974616 1106 58-59 0.25690529914491833 1106 60-61 0.3135826325763702 1106 62-63 0.319748728916629 1106 64-65 0.3554491288088357 1106 66-67 0.27422940253444494 1106 68-69 0.27676204538872895 1106 70-71 0.21558354807784497 1106 72-73 0.3149239683056564 1106 74-75 0.3084487416706878 1106 76-77 0.22702114477272062 1106 78-79 0.2585053520566163 1106 80-81 0.21722043672117763 1106 82-83 0.23826098816472552 1106 84-85 0.25228031739134593 1106 86-87 0.22519913674258163 1106 88-89 0.22397498494844825 1106 90-91 0.2439590424352147 1106 92-93 0.2691533703062632 1106 94-95 0.27424057529945145 1106 96-97 0.2291133744945526 1106 98-99 0.28209978496120414 1106 100 0.2977676953946826 1107 1 -8.048219161835846E-4 1107 2 -0.0015090761368767858 1107 3 0.01598885137830308 1107 4 0.025935629295311458 1107 5 0.06444195945042708 1107 6 0.05570027840867908 1107 7 0.08963375561911846 1107 8 0.021736639585327566 1107 9 0.028947688929164883 1107 10-11 0.016062173469052254 1107 12-13 0.03268506715736663 1107 14-15 0.09116989588235924 1107 16-17 0.049348183494309694 1107 18-19 0.06513679910196402 1107 20-21 0.004848930856333311 1107 22-23 0.11715984902680532 1107 24-25 0.13152217781213693 1107 26-27 0.0934991156552627 1107 28-29 0.10093965416115225 1107 30-31 0.035211107313998014 1107 32-33 0.016993080796709137 1107 34-35 -0.0024240320448001285 1107 36-37 0.029854457817293678 1107 38-39 0.013278205878897609 1107 40-41 0.04184325498526675 1107 42-43 0.048119014441375896 1107 44-45 0.09441179428382895 1107 46-47 0.0943303109860878 1107 48-49 0.07190610134733078 1107 50-51 0.125174430625151 1107 52-53 0.0802664157924653 1107 54-55 0.08767783569469145 1107 56-57 0.06408703757430345 1107 58-59 0.06660303048017369 1107 60-61 0.06731933449562177 1107 62-63 0.08464893092950376 1107 64-65 0.12649034883789056 1107 66-67 0.07752504164657381 1107 68-69 0.09349785066449101 1107 70-71 0.0842727080952912 1107 72-73 0.09231716915792276 1107 74-75 0.056138049341484475 1107 76-77 0.03677641141898391 1107 78-79 0.025614373840845417 1107 80-81 0.059452221124203675 1107 82-83 0.10856113689040825 1107 84-85 0.08272726158580213 1107 86-87 0.06108657045324861 1107 88-89 0.04302808158549709 1107 90-91 0.056271021575675206 1107 92-93 0.0831153136882179 1107 94-95 0.1061133228642035 1107 96-97 0.018097775207571942 1107 98-99 3.3719998835124443E-4 1107 100 -0.0018366101438047622 1108 1 -0.0581481206293617 1108 2 -0.027447973474359344 1108 3 -0.02058228226767511 1108 4 -0.013248671666865164 1108 5 -0.015866017209326344 1108 6 0.005510855443141338 1108 7 0.01145832759219445 1108 8 -7.414847735702779E-4 1108 9 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54-55 -0.27199446474828193 2214 56-57 -0.2840368916702829 2214 58-59 -0.29608152569883117 2214 60-61 -0.27620627568928313 2214 62-63 -0.23309046049246263 2214 64-65 -0.28642105936808804 2214 66-67 -0.24424261712673712 2214 68-69 -0.3019278684660449 2214 70-71 -0.2365207453059881 2214 72-73 -0.25767131853775993 2214 74-75 -0.2838607925536394 2214 76-77 -0.2683474384976563 2214 78-79 -0.2378003543563807 2214 80-81 -0.25824921694341896 2214 82-83 -0.214989370311244 2214 84-85 -0.3037324172277778 2214 86-87 -0.2943542986843255 2214 88-89 -0.2562140695441215 2214 90-91 -0.26874295993487607 2214 92-93 -0.2892321601099823 2214 94-95 -0.31972890827121603 2214 96-97 -0.23801406574977335 2214 98-99 -0.24560806603093255 2214 100 -0.25528431427441944 2215 1 -0.2051489045055419 2215 2 -0.19031058464896233 2215 3 -0.21258966536533563 2215 4 -0.11244380695014655 2215 5 -0.20107883335675325 2215 6 -0.1777045517238065 2215 7 -0.19213527473711167 2215 8 -0.17195144952195562 2215 9 -0.20866705862079016 2215 10-11 -0.19890846761443015 2215 12-13 -0.18039364628526044 2215 14-15 -0.34310516041826133 2215 16-17 -0.3430028026554126 2215 18-19 -0.29983952405197556 2215 20-21 -0.30827413689041094 2215 22-23 -0.32446040112711927 2215 24-25 -0.2952827877585449 2215 26-27 -0.29511462061035587 2215 28-29 -0.3353704682766008 2215 30-31 -0.31443194688270637 2215 32-33 -0.2587999173400064 2215 34-35 -0.2361536406308744 2215 36-37 -0.3110971554715505 2215 38-39 -0.30160690917681876 2215 40-41 -0.3212442144347847 2215 42-43 -0.2970878796799141 2215 44-45 -0.3222357251508754 2215 46-47 -0.3908431334384517 2215 48-49 -0.4030558862222833 2215 50-51 -0.3945470113042475 2215 52-53 -0.35372501825344926 2215 54-55 -0.36291105036178806 2215 56-57 -0.2660790900201562 2215 58-59 -0.33479966468419065 2215 60-61 -0.36490623314477233 2215 62-63 -0.2950638109116852 2215 64-65 -0.3064164152167663 2215 66-67 -0.31664773117586265 2215 68-69 -0.287918347955852 2215 70-71 -0.27157265202843206 2215 72-73 -0.19075120788440358 2215 74-75 -0.21949845898585352 2215 76-77 -0.22899572761972564 2215 78-79 -0.24445730718572634 2215 80-81 -0.22558966629193833 2215 82-83 -0.24348971795070184 2215 84-85 -0.24724312954867145 2215 86-87 -0.24381111036156966 2215 88-89 -0.23020983229356062 2215 90-91 -0.21731570312056903 2215 92-93 -0.1954348703270341 2215 94-95 -0.17250558936395777 2215 96-97 -0.19555915876582475 2215 98-99 -0.16501625118635843 2215 100 -0.1996454733664308 2216 1 -0.18577567620763347 2216 2 -0.19133403861590637 2216 3 -0.16821587768069435 2216 4 -0.17381618895808515 2216 5 -0.19084568593871154 2216 6 -0.14579056899468412 2216 7 -0.15719811748579104 2216 8 -0.20622211612696617 2216 9 -0.24007510712683455 2216 10-11 -0.24250416078409387 2216 12-13 -0.2608314198564514 2216 14-15 -0.3148340035418613 2216 16-17 -0.33930520662610775 2216 18-19 -0.2907644563413214 2216 20-21 -0.296305249731887 2216 22-23 -0.2975104908527584 2216 24-25 -0.37892206834245457 2216 26-27 -0.3129472978451986 2216 28-29 -0.3185669880458306 2216 30-31 -0.3167654348553981 2216 32-33 -0.3024405603413811 2216 34-35 -0.325722047661543 2216 36-37 -0.3080119014527938 2216 38-39 -0.37885356789771407 2216 40-41 -0.3117795898031801 2216 42-43 -0.31429983653404037 2216 44-45 -0.29707189942296 2216 46-47 -0.23207985546203247 2216 48-49 -0.26131887356621775 2216 50-51 -0.2765793105428358 2216 52-53 -0.2461774989720098 2216 54-55 -0.240669634959211 2216 56-57 -0.21993351322779375 2216 58-59 -0.23151029666192358 2216 60-61 -0.24690949399650464 2216 62-63 -0.3316188637650299 2216 64-65 -0.4267973849298343 2216 66-67 -0.3627882361951791 2216 68-69 -0.374356153349936 2216 70-71 -0.38442231224449586 2216 72-73 -0.337818728597874 2216 74-75 -0.47495896852004194 2216 76-77 -0.3932647633016444 2216 78-79 -0.4746842292509754 2216 80-81 -0.4299735676887195 2216 82-83 -0.38883199053265116 2216 84-85 -0.5148462344682443 2216 86-87 -0.49197294069485054 2216 88-89 -0.5615391688708442 2216 90-91 -0.5057260553054803 2216 92-93 -0.48956088698134437 2216 94-95 -0.4049565833568849 2216 96-97 -0.5069407395778214 2216 98-99 -0.577347919287547 2216 100 -0.51899935931419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 4.0 4 14.0 5 17.0 6 58.0 7 159.0 8 396.0 9 804.0 10 1469.0 11 2272.0 12 3625.0 13 5354.0 14 6372.0 15 8595.0 16 10230.0 17 14425.0 18 16191.0 19 8488.0 20 6244.0 21 5957.0 22 6331.0 23 6938.0 24 8052.0 25 10031.0 26 12764.0 27 16728.0 28 22132.0 29 28459.0 30 37274.0 31 47462.0 32 60586.0 33 78465.0 34 109488.0 35 163564.0 36 291316.0 37 656429.0 38 1211776.0 39 555144.0 40 1786.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41196400791687 20.133492561306138 13.731362257405083 23.723181173371906 2 15.51532205922794 20.519670106070038 40.17081675484558 23.794191079856436 3 17.96305600743939 25.793919888315397 30.488280495131953 25.754743609113255 4 12.144584437300843 17.238846109588728 39.29019512884981 31.32637432426062 5 12.41972786307249 37.93959910558401 36.24955459266566 13.39111843867784 6 30.42112585992453 38.87810445345457 17.68856268167626 13.01220700494464 7 26.55223268360339 32.81363135826088 23.08586442834686 17.548271529788877 8 24.891711263609658 36.40366621127913 20.556150571850022 18.148471953261193 9 24.891411566194368 16.346691284436844 21.529130100874884 37.232767048493905 10-11 23.643724411862618 26.820569531952472 29.348793889795076 20.186912166389835 12-13 24.630286942831905 24.126424898379963 29.439872531013034 21.8034156277751 14-15 21.6728235635477 26.025842863421122 26.880703376548237 25.42063019648294 16-17 20.90947473085962 29.497635862858086 27.429846656815243 22.16304274946705 18-19 20.745608127891316 28.145209931486797 30.024155296597765 21.085026644024126 20-21 21.98995525731327 27.439492407738612 29.58413441866641 20.98641791628171 22-23 21.387526438946235 27.29919283624601 29.69177107561714 21.621509649190614 24-25 21.39604725283911 27.458725939476864 29.682832453219216 21.462394354464806 26-27 21.177516537512112 27.354068331919645 29.757859728402124 21.71055540216612 28-29 21.18609615319019 27.402471940082236 29.814960965938397 21.596470940789178 30-31 21.467870111359723 27.930929684155792 29.352219845418624 21.248980359065857 32-33 21.132180268365317 27.52185017416604 29.83161070427962 21.514358853189023 34-35 21.273641682030814 27.375074624811774 29.813384536421445 21.537899156735968 36-37 21.63942403552829 27.702442950630658 29.179977625000035 21.478155388841017 38-39 21.54619974884487 27.8789073902792 29.203605765500512 21.371287095375415 40-41 21.351121115425048 27.657542691365077 29.419875854050552 21.57146033915932 42-43 21.38762476873262 27.526212742858196 29.273743572896908 21.812418915512268 44-45 21.54125160046249 27.704082573763277 29.08086826242988 21.67379756334435 46-47 21.62692190914204 27.90925505772498 28.806041074316212 21.657781958816766 48-49 21.662612991261103 27.850755646671754 28.623354850459048 21.863276511608092 50-51 21.6866949560215 27.884763187181427 28.46243755158591 21.96610430521116 52-53 21.747390290913064 27.802796670042536 28.493400077706426 21.95641296133798 54-55 21.82557981905002 28.180085664452882 28.257323625593227 21.737010890903868 56-57 21.564206324319212 27.95204629358799 28.412136256195797 22.071611125896997 58-59 21.44773452393396 27.5289796068945 28.833797551632756 22.189488317538785 60-61 21.663330326381626 27.958795099517804 28.44093213746249 21.93694243663808 62-63 21.57909468794312 27.923879910488285 28.4363499763865 22.060675425182094 64-65 21.524811450659495 27.650443474138896 28.590108563546668 22.23463651165494 66-67 21.492589928205003 27.797672847753084 28.44340621165368 22.266331012388232 68-69 21.861966246522012 27.852035962875334 28.43255174853041 21.853446042072243 70-71 21.779179005322334 27.718100683430258 28.412276330452084 22.090443980795325 72-73 21.71779234509972 27.890453465721656 28.403584111644676 21.98817007753395 74-75 21.72108564291181 27.894348972042625 28.278870438547127 22.10569494649843 76-77 21.743001626301435 27.8504230155104 28.152391866472588 22.254183491715583 78-79 21.873846225016607 27.95567688766737 28.22684420271715 21.943632684598875 80-81 21.84177109748467 27.699456494210523 28.353695403111594 22.105077005193213 82-83 21.596836928577776 27.82081062196111 28.4934815378924 22.088870911568716 84-85 21.585170077229176 27.787366645205168 28.242362064508043 22.385101213057606 86-87 21.69809635823143 27.868645585101138 28.19352690943172 22.239731147235712 88-89 21.473672498678756 27.679765880167306 28.3112101412888 22.535351479865138 90-91 21.442543041424678 27.60864317161656 28.422880451117145 22.52593333584162 92-93 21.606547993315566 27.788478500672326 28.27110167996551 22.333871826046604 94-95 21.341175264976282 27.592346430871935 28.322509808514372 22.743968495637407 96-97 21.35371329909443 27.556989447542655 28.37318649311225 22.716110760250665 98-99 21.252857631398342 27.642313557118666 28.294152161845 22.810676649637994 100 21.380949160264084 27.49750469854504 28.214576308206986 22.90696983298389 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 23.0 2 74.0 3 125.0 4 88.5 5 73.0 6 182.0 7 403.0 8 703.0 9 896.0 10 812.0 11 701.5 12 780.5 13 945.5 14 1127.0 15 1338.0 16 1586.0 17 1875.0 18 2306.5 19 2800.5 20 3399.5 21 4353.5 22 5637.5 23 7557.0 24 10435.0 25 14030.5 26 18810.0 27 25233.5 28 33502.0 29 42633.0 30 51881.0 31 62134.0 32 72828.5 33 84633.5 34 96032.0 35 105341.5 36 113174.5 37 119674.5 38 124801.0 39 127494.0 40 129131.0 41 129920.5 42 130089.5 43 130330.5 44 128512.0 45 125924.0 46 122273.0 47 116540.5 48 112790.5 49 110534.0 50 107197.5 51 102212.5 52 95027.5 53 90659.5 54 87421.0 55 81286.0 56 73715.5 57 67101.5 58 60343.5 59 51606.0 60 44041.5 61 37735.5 62 31672.0 63 25430.0 64 19966.5 65 15952.5 66 12371.5 67 9098.0 68 6978.5 69 5721.5 70 4345.5 71 2924.5 72 2076.5 73 1560.0 74 1123.5 75 634.5 76 298.0 77 204.5 78 125.5 79 52.0 80 29.0 81 11.0 82 4.0 83 2.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042337411603085885 2 0.23838949188957492 3 0.002547271652467823 4 0.0025179926679566988 5 1.7567390706674642E-4 6 8.783695353337321E-5 7 0.0 8 3.8062679864461725E-4 9 0.0 10-11 5.270217212002393E-4 12-13 0.0 14-15 2.3423187608899524E-4 16-17 2.3423187608899524E-4 18-19 5.563007057113637E-4 20-21 1.4639492255562203E-5 22-23 8.783695353337321E-5 24-25 0.0013907517642784092 26-27 5.855796902224881E-5 28-29 1.3175543030005983E-4 30-31 2.9278984511124405E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.0247644578893541E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3423187608899524E-4 70-71 2.9278984511124405E-5 72-73 3.5134781413349284E-4 74-75 1.46394922555622E-4 76-77 1.4639492255562203E-5 78-79 0.0 80-81 0.0 82-83 1.3175543030005983E-4 84-85 0.0 86-87 5.270217212002393E-4 88-89 0.002166644853823206 90-91 0.0022105633305898927 92-93 6.294981669891747E-4 94-95 5.563007057113637E-4 96-97 1.7567390706674642E-4 98-99 8.783695353337321E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3415419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.33534850828365 #Duplication Level Percentage of deduplicated Percentage of total 1 73.83321176197019 44.54752563144379 2 15.454901109882673 18.64953689251662 3 4.9948736285315025 9.041023233968506 4 2.171920794306966 5.241743922275962 5 1.105661358273848 3.335523169179744 6 0.6527985305539469 2.3632096108000704 7 0.40992237412352545 1.731296651286016 8 0.26212183995059446 1.2652170052041332 9 0.20041428667785613 1.0882859249472767 >10 0.8534910560603383 8.668568196775338 >50 0.04417555272145334 1.804737768006609 >100 0.015683494431074717 1.677083831887892 >500 6.302801621565001E-4 0.2528580985629958 >1k 1.939323538522348E-4 0.3333900631449563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4424 0.12953022747721435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3913 0.1145686663920298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.9278984511124405E-5 0.0 14-15 0.0 0.0 0.0 2.9278984511124405E-5 0.0 16-17 0.0 0.0 0.0 2.9278984511124405E-5 0.0 18-19 0.0 0.0 0.0 2.9278984511124405E-5 0.0 20-21 0.0 0.0 0.0 2.9278984511124405E-5 0.0 22-23 0.0 0.0 0.0 7.3197461277811E-5 0.0 24-25 0.0 0.0 0.0 1.1711593804449762E-4 0.0 26-27 0.0 0.0 0.0 1.610344148111842E-4 0.0 28-29 0.0 0.0 0.0 6.002191824780503E-4 0.0 30-31 0.0 0.0 0.0 0.0025033531757011366 0.0 32-33 0.0 0.0 0.0 0.007641814957403469 0.0 34-35 0.0 0.0 0.0 0.01490300311616232 0.0 36-37 0.0 0.0 0.0 0.029015473650524287 0.0 38-39 0.0 0.0 0.0 0.05216051090656812 0.0 40-41 0.0 0.0 0.0 0.08091247369649229 0.0 42-43 0.0 0.0 0.0 0.11150901251061729 0.0 44-45 0.0 0.0 0.0 0.14711225767614455 0.0 46-47 0.0 0.0 0.0 0.19088433952027556 0.0 48-49 0.0 0.0 0.0 0.24100996100332053 0.0 50-51 0.0 0.0 0.0 0.2981918177535465 0.0 52-53 0.0 0.0 0.0 0.35467097887550547 0.0 54-55 0.0 0.0 0.0 0.41775255100472297 0.0 56-57 0.0 0.0 0.0 0.49436101397807997 0.0 58-59 0.0 0.0 0.0 0.5821686885269421 0.0 60-61 0.0 0.0 0.0 0.6790674877665083 0.0 62-63 0.0 0.0 0.0 0.7757320551299856 0.0 64-65 0.0 0.0 0.0 0.8835519155922011 0.0 66-67 0.0 0.0 0.0 1.0045619585766783 0.0 68-69 0.0 0.0 0.0 1.142509894100841 0.0 70-71 0.0 0.0 0.0 1.2993866931114453 0.0 72-73 0.0 0.0 0.0 1.4585765318984287 0.0 74-75 0.0 0.0 0.0 1.625627778026649 0.0 76-77 0.0 0.0 0.0 1.8071428425033649 0.0 78-79 0.0 0.0 0.0 2.0188738189955613 0.0 80-81 0.0 0.0 0.0 2.251612466874489 0.0 82-83 0.0 0.0 0.0 2.4962383824649335 0.0 84-85 0.0 0.0 0.0 2.740776461101844 0.0 86-87 0.0 0.0 0.0 3.0048436224076753 0.0 88 0.0 0.0 0.0 3.2239968214734414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4550 0.0 44.23038 1 GTATCAA 7690 0.0 43.208282 1 TCAACGC 9000 0.0 36.13697 4 ATCAACG 9050 0.0 35.93679 3 CAACGCA 9135 0.0 35.654896 5 AACGCAG 9385 0.0 34.80527 6 TATCAAC 9760 0.0 33.59384 2 ACGCAGA 11000 0.0 29.438862 7 CGCAGAG 11250 0.0 28.65933 8 GTACATG 8685 0.0 25.071272 1 GCAGAGT 12800 0.0 24.674446 9 TACATGG 8580 0.0 24.213165 2 ACATGGG 8715 0.0 22.973331 3 GTGGTAT 2325 0.0 21.28485 1 AGAGTAC 11835 0.0 20.848557 10-11 GAGTACT 8875 0.0 20.599197 12-13 CAGAGTA 12855 0.0 19.413658 10-11 CATGGGG 6490 0.0 19.118216 4 GAGTACA 6290 0.0 19.107056 1 AGTACTT 9490 0.0 18.744278 12-13 >>END_MODULE