FastQCFastQC Report
Wed 25 May 2016
SRR1033057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800517
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.035.5330851
GTATCAA34250.033.0830271
TCAACGC33200.032.288334
ATCAACG34800.031.0740153
CAACGCA34800.030.938915
AACGCAG36800.028.9951846
GTACATG74750.026.8574941
TATCAAC42000.026.0828722
TACATGG73050.026.002192
ACGCAGA43650.024.014227
ACATGGG76600.023.99923
CGCAGAG44150.023.2273628
CATGGGG53750.018.8940074
GCAGAGT55750.017.8903759
GAGTACA52850.016.7248271
ATAACCG2253.8652244E-516.7169195
AGAGTAC46850.016.27410510-11
GTGTAGG12750.016.2252451
GTATAGA14050.015.7278851
CTAGTAC6900.014.9907163
Produced by FastQC (version 0.11.5)