##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033057_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2800517
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486630861373097	33.0	31.0	34.0	30.0	34.0
2	31.502016591936417	33.0	31.0	34.0	28.0	34.0
3	31.685073506070488	34.0	31.0	34.0	30.0	34.0
4	35.09903778480902	37.0	35.0	37.0	33.0	37.0
5	35.12495192851891	37.0	35.0	37.0	33.0	37.0
6	35.19794809315566	37.0	35.0	37.0	33.0	37.0
7	35.0660474476677	37.0	35.0	37.0	32.0	37.0
8	35.04043324857518	37.0	35.0	37.0	32.0	37.0
9	36.66065730006281	39.0	37.0	39.0	33.0	39.0
10-11	36.69491918813562	39.0	37.0	39.0	33.0	39.0
12-13	36.64925440552584	39.0	37.0	39.0	33.0	39.0
14-15	38.006412387427034	40.0	38.0	41.0	33.0	41.0
16-17	37.9966509755163	40.0	38.0	41.0	33.0	41.0
18-19	37.99253852056603	40.0	38.0	41.0	33.0	41.0
20-21	38.035755897928844	40.0	38.5	41.0	33.0	41.0
22-23	37.97814135747078	40.0	38.0	41.0	33.0	41.0
24-25	37.980808722103816	40.0	38.0	41.0	33.0	41.0
26-27	37.90126198126988	40.0	38.0	41.0	33.0	41.0
28-29	37.9171726149136	40.0	38.0	41.0	32.5	41.0
30-31	38.070658739082816	40.0	38.0	41.0	33.0	41.0
32-33	38.064904801506295	40.0	38.0	41.0	33.0	41.0
34-35	38.0190398772798	40.0	38.0	41.0	33.0	41.0
36-37	37.93671222135056	40.0	38.0	41.0	33.0	41.0
38-39	37.84754564960684	40.0	38.0	41.0	32.5	41.0
40-41	37.777850304068856	40.0	38.0	41.0	32.0	41.0
42-43	37.64748901720647	40.0	38.0	41.0	32.0	41.0
44-45	37.49492254465872	40.0	38.0	41.0	31.5	41.0
46-47	37.35971411707195	40.0	38.0	41.0	31.0	41.0
48-49	37.24393995822914	40.0	37.0	41.0	31.0	41.0
50-51	37.11536887653244	40.0	37.0	41.0	31.0	41.0
52-53	36.96451405222679	40.0	37.0	41.0	30.5	41.0
54-55	36.81030520436048	40.0	36.0	41.0	30.0	41.0
56-57	36.63917930153611	40.0	36.0	41.0	30.0	41.0
58-59	36.44315603154703	39.0	35.5	41.0	30.0	41.0
60-61	36.21995188745507	39.0	35.0	41.0	30.0	41.0
62-63	35.96977165287695	39.0	35.0	41.0	29.5	41.0
64-65	35.69697345168767	38.5	35.0	40.0	29.0	41.0
66-67	35.402342138969345	38.0	35.0	40.0	29.0	41.0
68-69	35.09254094868912	37.0	34.0	40.0	28.5	41.0
70-71	34.76057849318536	37.0	34.0	39.5	28.0	41.0
72-73	34.411081418181	36.0	34.0	39.0	28.0	41.0
74-75	34.05987198078069	36.0	34.0	39.0	28.0	40.5
76-77	33.69209221011692	35.5	34.0	38.5	27.0	40.0
78-79	33.30051897560344	35.0	33.5	37.0	27.0	39.0
80-81	32.94432242332398	35.0	33.0	37.0	26.0	39.0
82-83	32.71267340994538	35.0	33.0	36.5	26.0	39.0
84-85	32.48169712949431	35.0	33.0	36.0	26.0	37.0
86-87	32.293789503866606	35.0	33.0	36.0	26.0	37.0
88-89	32.06108747063489	35.0	33.0	35.5	26.0	37.0
90-91	31.83893045462677	35.0	33.0	35.0	26.0	36.0
92-93	31.60540643031269	35.0	33.0	35.0	25.0	36.0
94-95	31.39133345735805	35.0	33.0	35.0	25.0	36.0
96-97	31.203283893652493	35.0	33.0	35.0	24.5	36.0
98-99	31.015919739105314	35.0	32.5	35.0	24.0	35.0
100	30.86232149278151	35.0	32.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22398482014708776
1101	2	0.22292775736672255
1101	3	0.2454023984545053
1101	4	0.16202193765963102
1101	5	0.19352182903678283
1101	6	0.2933587656735597
1101	7	0.3178127794987873
1101	8	0.32232763239202455
1101	9	0.4314714599000382
1101	10-11	0.44869576311847936
1101	12-13	0.4370175843344768
1101	14-15	0.5890989002045046
1101	16-17	0.5279464965233558
1101	18-19	0.5585936957405195
1101	20-21	0.45255338689440805
1101	22-23	0.5477640760019824
1101	24-25	0.5237696222424191
1101	26-27	0.4434936313761355
1101	28-29	0.4368385308556739
1101	30-31	0.44907944165937863
1101	32-33	0.47230131214824667
1101	34-35	0.45577765902036305
1101	36-37	0.4399276503644316
1101	38-39	0.45711729217022423
1101	40-41	0.4851367909670614
1101	42-43	0.5233409231113768
1101	44-45	0.5326462617961454
1101	46-47	0.4612711988328897
1101	48-49	0.47252982123013254
1101	50-51	0.4722216180922132
1101	52-53	0.4088596043648991
1101	54-55	0.396237569605141
1101	56-57	0.46262205496577025
1101	58-59	0.48041598597188795
1101	60-61	0.4721538463422803
1101	62-63	0.4788072594621795
1101	64-65	0.44606712836699813
1101	66-67	0.43877941661604325
1101	68-69	0.4223261859527625
1101	70-71	0.45848443658329785
1101	72-73	0.38418118525987666
1101	74-75	0.3066207732272943
1101	76-77	0.33649727381609296
1101	78-79	0.32591621103627944
1101	80-81	0.3540086941956986
1101	82-83	0.3351866649359394
1101	84-85	0.37495529051378895
1101	86-87	0.37272706488494123
1101	88-89	0.43013205557398493
1101	90-91	0.4186539701286556
1101	92-93	0.4540824611074541
1101	94-95	0.46085255379897205
1101	96-97	0.4641039447802022
1101	98-99	0.37804766743036566
1101	100	0.34250095130216707
1102	1	0.10512641127604994
1102	2	0.19655258614171345
1102	3	0.1568041936418645
1102	4	-0.01582156347593866
1102	5	0.08739971826691573
1102	6	0.15130655210175803
1102	7	0.3032586365193666
1102	8	0.2330523928218966
1102	9	0.2411407967592254
1102	10-11	0.2843847813842757
1102	12-13	0.22324808551957886
1102	14-15	0.2980142467810296
1102	16-17	0.32610769137207285
1102	18-19	0.318191092913402
1102	20-21	0.35472345129170435
1102	22-23	0.32981714923474925
1102	24-25	0.34097310095780387
1102	26-27	0.2733409911889524
1102	28-29	0.3017428787393541
1102	30-31	0.42629507981690296
1102	32-33	0.39253019032656766
1102	34-35	0.4159347534380444
1102	36-37	0.4082334131424332
1102	38-39	0.4841448674171218
1102	40-41	0.38174682901982493
1102	42-43	0.47026976015567357
1102	44-45	0.4296259333670207
1102	46-47	0.3629756940318245
1102	48-49	0.29193685386399437
1102	50-51	0.3751026276294809
1102	52-53	0.37893063885441336
1102	54-55	0.43425404059889416
1102	56-57	0.3834248223841499
1102	58-59	0.33533760710757576
1102	60-61	0.2866679867993014
1102	62-63	0.36305822223416584
1102	64-65	0.35280219258560663
1102	66-67	0.30626019463819887
1102	68-69	0.34147900455753444
1102	70-71	0.30244500091277615
1102	72-73	0.35811779637146657
1102	74-75	0.3267319758239964
1102	76-77	0.29367353177399025
1102	78-79	0.32882232736538697
1102	80-81	0.37595212684816204
1102	82-83	0.34463469084112575
1102	84-85	0.26771537900781084
1102	86-87	0.27945947284775485
1102	88-89	0.33879211182036784
1102	90-91	0.3761321098333532
1102	92-93	0.3448924093856718
1102	94-95	0.43542587287545587
1102	96-97	0.40971393357149566
1102	98-99	0.4327592580749915
1102	100	0.36064938784380374
1103	1	0.13064307676923548
1103	2	0.1788731408312323
1103	3	0.1987445278005815
1103	4	0.23542727099567173
1103	5	0.1741601053703974
1103	6	0.19081344064598937
1103	7	0.2674557758175311
1103	8	0.24343755512727938
1103	9	0.2829399843604392
1103	10-11	0.27524983540491377
1103	12-13	0.2936449804269188
1103	14-15	0.42781229204926774
1103	16-17	0.37829967437920686
1103	18-19	0.320916899439311
1103	20-21	0.39680318823926797
1103	22-23	0.38261152206038673
1103	24-25	0.3684667065910361
1103	26-27	0.32290547674101333
1103	28-29	0.3637679403141263
1103	30-31	0.42290114668417544
1103	32-33	0.362223918747425
1103	34-35	0.2369395655815154
1103	36-37	0.3721651047888628
1103	38-39	0.4321747049439395
1103	40-41	0.41692245942509487
1103	42-43	0.32486366933625277
1103	44-45	0.35832188394137177
1103	46-47	0.43920776529339633
1103	48-49	0.39510327453864136
1103	50-51	0.40648625212831035
1103	52-53	0.44322167844980953
1103	54-55	0.4871172758359208
1103	56-57	0.47961034489291166
1103	58-59	0.4239193112235853
1103	60-61	0.45364541716864437
1103	62-63	0.3995511218055938
1103	64-65	0.38684226767952623
1103	66-67	0.4598007359434746
1103	68-69	0.4494989585352087
1103	70-71	0.41351253608590355
1103	72-73	0.4377907838156716
1103	74-75	0.4153820488319653
1103	76-77	0.3712707764879042
1103	78-79	0.34514067898415846
1103	80-81	0.339175190393064
1103	82-83	0.2899815922300206
1103	84-85	0.29001604157409844
1103	86-87	0.2194687312723005
1103	88-89	0.2933837837128621
1103	90-91	0.32505748772117826
1103	92-93	0.30917976403597436
1103	94-95	0.28216561770616977
1103	96-97	0.3358119320540851
1103	98-99	0.33938126891004927
1103	100	0.3913946614082029
1104	1	0.11010739847712969
1104	2	0.21717008521316217
1104	3	0.19913534015510237
1104	4	0.24570710876664492
1104	5	0.1458582694855579
1104	6	0.22994895559173756
1104	7	0.3085742381143888
1104	8	0.21562728195541325
1104	9	0.3312364176898228
1104	10-11	0.28231308942564226
1104	12-13	0.4274892922375315
1104	14-15	0.4542155836085513
1104	16-17	0.4249357583073703
1104	18-19	0.4912492200844625
1104	20-21	0.48396491351456206
1104	22-23	0.4501658118703489
1104	24-25	0.3651327712102983
1104	26-27	0.200820420456985
1104	28-29	0.2586422420211463
1104	30-31	0.2834071331409618
1104	32-33	0.3120922989108408
1104	34-35	0.290577418513287
1104	36-37	0.4197471755420281
1104	38-39	0.4179141805151545
1104	40-41	0.5199844894591195
1104	42-43	0.5041163327611713
1104	44-45	0.48063910409487676
1104	46-47	0.5286809399549739
1104	48-49	0.4506597329889601
1104	50-51	0.4223659243309541
1104	52-53	0.39191035254144424
1104	54-55	0.4616183568231449
1104	56-57	0.5302790460354103
1104	58-59	0.48168069158602833
1104	60-61	0.4564119889996192
1104	62-63	0.5187275590456508
1104	64-65	0.482191879993799
1104	66-67	0.546127096636738
1104	68-69	0.49999625075028575
1104	70-71	0.46799089586752984
1104	72-73	0.4678278494091046
1104	74-75	0.48408369921425987
1104	76-77	0.5047247203375207
1104	78-79	0.4701666363268231
1104	80-81	0.4823376406646389
1104	82-83	0.4677582660778796
1104	84-85	0.5228979239996363
1104	86-87	0.4192280604412417
1104	88-89	0.4444316160089059
1104	90-91	0.44817229900789
1104	92-93	0.49252611704122984
1104	94-95	0.5387896046079845
1104	96-97	0.5770134007111736
1104	98-99	0.5339526212090391
1104	100	0.4791446049861001
1105	1	0.08622019596022312
1105	2	0.18046019968420168
1105	3	0.15365960013298618
1105	4	0.13111216511297386
1105	5	0.1222872047741177
1105	6	0.21483835807548957
1105	7	0.202176040166691
1105	8	0.24080685339159658
1105	9	0.23029789838455628
1105	10-11	0.3048146608522302
1105	12-13	0.2781690249616915
1105	14-15	0.3051819054268776
1105	16-17	0.32908268458238865
1105	18-19	0.34448839354900684
1105	20-21	0.31248192837923483
1105	22-23	0.2820291803299
1105	24-25	0.2587480873186436
1105	26-27	0.29580434298190283
1105	28-29	0.3014238866410679
1105	30-31	0.3657465456129714
1105	32-33	0.32156559146148567
1105	34-35	0.3001279993170485
1105	36-37	0.35164495777306826
1105	38-39	0.34542284029189574
1105	40-41	0.3738781683975816
1105	42-43	0.3309906457840057
1105	44-45	0.33154587296060356
1105	46-47	0.33286159974337437
1105	48-49	0.2760148231629387
1105	50-51	0.19473944628042972
1105	52-53	0.2876923514422174
1105	54-55	0.21298344351141196
1105	56-57	0.2784053145725238
1105	58-59	0.24282546028885577
1105	60-61	0.2779838723287895
1105	62-63	0.27804707985969657
1105	64-65	0.27592723736222524
1105	66-67	0.21619891063425456
1105	68-69	0.3452814759876972
1105	70-71	0.1507183955867788
1105	72-73	0.2536988461129539
1105	74-75	0.24793686696062167
1105	76-77	0.21954051099876892
1105	78-79	0.27643544553926347
1105	80-81	0.25933549038092707
1105	82-83	0.2852438329141762
1105	84-85	0.301565716250515
1105	86-87	0.20878994324746714
1105	88-89	0.24971563014393894
1105	90-91	0.3118300200983022
1105	92-93	0.3273091897172762
1105	94-95	0.32722116569282633
1105	96-97	0.41783752975759114
1105	98-99	0.39310870394032804
1105	100	0.45721472368324356
1106	1	0.15900624281824705
1106	2	0.1614248262193847
1106	3	0.15065769362242065
1106	4	0.2168303753750891
1106	5	0.14835913519503663
1106	6	0.1881052489795607
1106	7	0.1870914527064258
1106	8	0.35320276542028495
1106	9	0.30322310109800554
1106	10-11	0.3638612548808169
1106	12-13	0.2923075438646663
1106	14-15	0.3638216091251749
1106	16-17	0.34643366943898
1106	18-19	0.35445988717354027
1106	20-21	0.4275266700874809
1106	22-23	0.3479384299633921
1106	24-25	0.3064534791298925
1106	26-27	0.33601041651774466
1106	28-29	0.3473593156581032
1106	30-31	0.2901079775910631
1106	32-33	0.2611442465412992
1106	34-35	0.2675544592726524
1106	36-37	0.31414949656645064
1106	38-39	0.33433389790827306
1106	40-41	0.3883481749786455
1106	42-43	0.2774142794441872
1106	44-45	0.2967896550672293
1106	46-47	0.1942378807118601
1106	48-49	0.3074711172993503
1106	50-51	0.30973677878844796
1106	52-53	0.3117423200437628
1106	54-55	0.3542112780440334
1106	56-57	0.3233212429336305
1106	58-59	0.3023351054240422
1106	60-61	0.26227401083842494
1106	62-63	0.30714578054043074
1106	64-65	0.3572761508192812
1106	66-67	0.3791751651741322
1106	68-69	0.40246317290669964
1106	70-71	0.3503490616889309
1106	72-73	0.3840011782920669
1106	74-75	0.4635111369792
1106	76-77	0.4162409639652793
1106	78-79	0.355561052969648
1106	80-81	0.46162704131379684
1106	82-83	0.39691003157437166
1106	84-85	0.40664229688803033
1106	86-87	0.4386144741470446
1106	88-89	0.49097742774664255
1106	90-91	0.45888428885340815
1106	92-93	0.4550439485722322
1106	94-95	0.4247972978845098
1106	96-97	0.320834944565199
1106	98-99	0.3769889673106057
1106	100	0.36415069547187073
1107	1	0.022940492501952292
1107	2	0.010729339616528222
1107	3	0.06935623702645444
1107	4	0.043657050440877754
1107	5	0.02704166731620461
1107	6	0.1361624543505613
1107	7	0.11660292675983897
1107	8	0.11759097581256839
1107	9	0.1396353989045096
1107	10-11	0.13397324714706116
1107	12-13	0.18548507022216398
1107	14-15	0.2322517173859424
1107	16-17	0.14052776062105465
1107	18-19	0.21173558026153216
1107	20-21	0.21404138807603346
1107	22-23	0.22015608494821493
1107	24-25	0.16870100561886403
1107	26-27	0.05672827248277912
1107	28-29	0.11394490958746673
1107	30-31	0.13872448694677786
1107	32-33	0.051789813969804754
1107	34-35	0.062388638718182676
1107	36-37	0.101524946883778
1107	38-39	0.15163551002963516
1107	40-41	0.12372175339687175
1107	42-43	0.17889814830048323
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2216	94-95	-0.9689143104466496
2216	96-97	-0.9394777788863671
2216	98-99	-0.8561845644709258
2216	100	-0.8473318641729399
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32386.0
3	6776.0
4	3671.0
5	2895.0
6	2565.0
7	2739.0
8	3286.0
9	3789.0
10	4323.0
11	4736.0
12	5388.0
13	5938.0
14	6627.0
15	6743.0
16	6546.0
17	6596.0
18	6479.0
19	6541.0
20	6601.0
21	6773.0
22	7978.0
23	11126.0
24	16866.0
25	21732.0
26	22209.0
27	20126.0
28	22799.0
29	27801.0
30	33744.0
31	41706.0
32	54016.0
33	72486.0
34	103849.0
35	156816.0
36	280563.0
37	570276.0
38	874011.0
39	330512.0
40	503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.359269062346364	20.2534929055715	13.768286303922803	23.618951728159335
2	15.036213440500113	20.414735432016077	40.93663764233087	23.612413485152935
3	17.625220708711662	25.873992207044243	30.3402576262854	26.1605294579587
4	11.840422924062203	17.200729554012938	40.03474100761072	30.924106514314136
5	12.5239314263005	38.35882861900169	36.17223693657729	12.945003018120516
6	30.061542420311145	39.003134303287624	18.304831544600386	12.63049173180085
7	26.703604652284845	32.80223214748413	23.233404443065695	17.260758757165327
8	24.34776734300843	37.29391092247984	20.803031635294445	17.555290099217284
9	24.45326397484337	17.44079610240933	21.59941040041375	36.50652952233355
10-11	23.215419625143177	27.48415071551211	29.519568256786915	19.780861402557797
12-13	23.929586597482665	24.83199669426687	29.463329525258402	21.77508718299206
14-15	21.30705733105182	26.912658499784992	26.35828234543043	25.42200182373276
16-17	21.01550004216629	30.20221237230404	26.870128542861675	21.912159042667998
18-19	20.96537745106712	29.242921144983725	28.716647964381842	21.075053439567316
20-21	22.221898576151876	28.460700769590648	28.1741369937823	21.143263660475178
22-23	21.807416940195083	28.24976589159307	28.26352121195448	21.679295956257366
24-25	21.755485747868374	28.291542473886256	28.185628525442475	21.7673432528029
26-27	21.47150109308842	28.251153318730204	28.29517018214325	21.982175406038127
28-29	21.466809469660767	28.24547941877157	28.420326737776364	21.867384373791303
30-31	21.73604867401475	28.74567784411794	27.9943302748884	21.523943206978917
32-33	21.42221477386606	28.380762110958628	28.502111555372206	21.694911559803103
34-35	21.520785765026943	28.16438392368927	28.50585784384631	21.808972467437474
36-37	21.840349162632396	28.561190365480734	27.972774232017805	21.62568623986907
38-39	21.68134777978688	28.6585989036514	28.122663826928434	21.537389489633288
40-41	21.41858175060051	28.361378585259946	28.414822788064882	21.805216876074667
42-43	21.453012817569274	28.1883071506767	28.418547051505232	21.940132980248787
44-45	21.588098906347067	28.390148100807387	28.344462349098105	21.67729064374744
46-47	21.597903163729836	28.608739343125194	28.194203574942172	21.599153918202802
48-49	21.525681080824178	28.406851385246405	28.226032676727954	21.84143485720146
50-51	21.571967125245667	28.30514561372164	28.196033589422903	21.92685367160979
52-53	21.537140174989812	28.34053062268092	28.255821245883574	21.866507956445695
54-55	21.67109351844226	28.687857550835844	28.085314994905563	21.55573393581634
56-57	21.554524987891117	28.413556874048506	28.131251528684963	21.90066660937541
58-59	21.390978041373167	28.07078914312064	28.44845344156173	22.089779373944467
60-61	21.692319455922902	28.48602986864357	28.0738951828353	21.747755492598227
62-63	21.524914205999934	28.4631153691679	28.137305418064408	21.874665006767756
64-65	21.53342696192606	28.19182415499778	28.237292562672707	22.037456320403454
66-67	21.358174588654997	28.313324690991	28.102399607807264	22.226101112546736
68-69	21.808794781173834	28.37888586479776	28.06773440071006	21.744584953318345
70-71	21.735543523912813	28.189076588788108	28.132106222489554	21.943273664809517
72-73	21.484308287713667	28.29220834437947	28.3137527841633	21.90973058374356
74-75	21.52452836930113	28.335697441760754	28.153119193940263	21.986654994997856
76-77	21.596613736197973	28.33596824529155	28.029246629545668	22.038171388964813
78-79	21.729363157938923	28.37593136394142	28.092442677497946	21.802262800621712
80-81	21.59512049436664	28.231313173893568	28.22073879204835	21.95282753969144
82-83	21.44777453022068	28.281184854594116	28.30363908601096	21.967401529174246
84-85	21.496655358597607	28.124030459447685	28.19758381340382	22.181730368550888
86-87	21.633734783959145	28.294669248420057	28.120059748741642	21.951536218879152
88-89	21.475831571449316	28.19365107319679	28.16814491072575	22.16237244462814
90-91	21.397860908697645	28.064329914878584	28.26422896514376	22.27358021128001
92-93	21.576335481096127	28.244533457266996	28.098999516672997	22.08013154496388
94-95	21.35070699810779	28.060464297158184	28.297660438719983	22.291168266014044
96-97	21.340675431863897	28.070559307796028	28.287060596247514	22.30170466409256
98-99	21.39770774058641	28.117785560305368	28.204896919415827	22.27960977969239
100	21.467806721359093	28.004602995233736	28.10095721865335	22.426633064753826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	389.0
1	353.0
2	324.5
3	315.5
4	303.0
5	306.0
6	300.0
7	278.5
8	240.0
9	216.5
10	216.0
11	242.0
12	256.5
13	251.5
14	287.0
15	346.5
16	431.5
17	586.0
18	812.5
19	1186.5
20	1742.5
21	2509.5
22	3798.0
23	6045.5
24	8776.0
25	12208.0
26	17203.5
27	23551.0
28	31599.5
29	40670.0
30	49132.0
31	58184.5
32	67000.0
33	75369.5
34	84296.5
35	91402.0
36	96325.5
37	100787.5
38	103672.0
39	104795.0
40	105622.5
41	105463.5
42	103233.0
43	100691.0
44	98302.5
45	95681.5
46	94008.5
47	90870.5
48	87569.0
49	85856.5
50	83501.5
51	80025.5
52	74848.5
53	70203.0
54	67805.5
55	65055.5
56	60259.5
57	54716.5
58	49354.5
59	44467.0
60	38933.0
61	32413.5
62	27235.0
63	22761.5
64	18734.0
65	15088.0
66	11546.5
67	8372.5
68	5973.5
69	4499.0
70	3249.5
71	2208.5
72	1579.0
73	1189.0
74	845.5
75	575.5
76	397.0
77	291.5
78	197.5
79	109.5
80	60.0
81	43.0
82	33.5
83	23.0
84	15.5
85	16.0
86	14.5
87	9.0
88	8.0
89	5.5
90	5.0
91	4.5
92	1.5
93	2.0
94	4.5
95	3.5
96	1.0
97	1.0
98	1.5
99	2.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060738785017195034
2	0.04077818488514799
3	0.05638244652683772
4	0.07480761587949653
5	0.08634120057118025
6	0.08737672365495372
7	0.0890549852045176
8	0.10065998528128914
9	0.09591086217294878
10-11	0.10124916220826369
12-13	0.10544481608217339
14-15	0.10562335454489297
16-17	0.07402204664353046
18-19	0.09749985449115288
20-21	0.09055470829136192
22-23	0.05713230807025989
24-25	0.021513884757707238
26-27	0.17844919348820237
28-29	0.06389891580733129
30-31	0.07659300050669215
32-33	0.07096903893102595
34-35	0.021281784756171807
36-37	0.052758115733630616
38-39	0.07630733896634086
40-41	0.08111002361349708
42-43	0.07611094665734934
44-45	0.07325433125383635
46-47	0.07877116975187082
48-49	0.07620021588870912
50-51	0.07202241586107136
52-53	0.07589670050208587
54-55	0.06700548505865167
56-57	0.0696835619994451
58-59	0.06668411582575645
60-61	0.06413101580886672
62-63	0.06973712353826098
64-65	0.06679123890338819
66-67	0.06759466198562622
68-69	0.06713046198255537
70-71	0.060470977323115696
72-73	0.058953400389999425
74-75	0.061310108097897636
76-77	0.05577541575359121
78-79	0.05225820803801584
80-81	0.046384292614542244
82-83	0.053204461890429516
84-85	0.04243859258843992
86-87	0.04026042334326126
88-89	0.04261713105115948
90-91	0.03942129256847932
92-93	0.04177800027637754
94-95	0.04092101565532364
96-97	0.043795484905108595
98-99	0.043795484905108595
100	0.05249030803955127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2800517.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.44739238049559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40273496835688	52.88060029114769
2	13.348847050946494	18.00689849744819
3	4.03656691613845	8.167677379887511
4	1.6371891188022407	4.416965475877301
5	0.8200636627748148	2.7655577820079666
6	0.4778573870003711	1.9338140809759417
7	0.28668545773132964	1.353533059017088
8	0.19571432138968306	1.05603365034019
9	0.147465173160492	0.8951527256952088
>10	0.6200442735164606	7.018663631268024
>50	0.021864726888966513	0.9710411138968854
>100	0.004913975811300683	0.5122699491115762
>500	5.2967482537318645E-5	0.02179236332641963
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.570769254391243E-5	0.0
5	0.0	0.0	0.0	3.570769254391243E-5	0.0
6	0.0	0.0	0.0	3.570769254391243E-5	0.0
7	0.0	0.0	0.0	7.141538508782486E-5	0.0
8	0.0	0.0	0.0	7.141538508782486E-5	0.0
9	0.0	0.0	0.0	7.141538508782486E-5	0.0
10-11	0.0	0.0	0.0	7.141538508782486E-5	0.0
12-13	0.0	0.0	0.0	1.0712307763173729E-4	0.0
14-15	0.0	0.0	0.0	1.6068461644760593E-4	0.0
16-17	0.0	0.0	0.0	2.1424615526347457E-4	0.0
18-19	0.0	0.0	0.0	2.49953847807387E-4	0.0
20-21	0.0	0.0	0.0	2.8566154035129945E-4	0.0
22-23	0.0	0.0	0.0	2.8566154035129945E-4	0.0
24-25	0.0	0.0	0.0	3.2136923289521186E-4	0.0
26-27	0.0	0.0	0.0	4.6420000307086155E-4	0.0
28-29	0.0	0.0	0.0	8.212769285099858E-4	0.0
30-31	0.0	0.0	0.0	0.002838761557241038	0.0
32-33	0.0	0.0	0.0	0.008087792361196165	0.0
34-35	0.0	0.0	0.0	0.014729423174363876	0.0
36-37	0.0	0.0	0.0	0.025406023244993692	0.0
38-39	0.0	0.0	0.0	0.04688420031015701	0.0
40-41	0.0	0.0	0.0	0.073397162024012	0.0
42-43	0.0	0.0	0.0	0.1021418545218615	0.0
44-45	0.0	0.0	0.0	0.13245768549164316	0.0
46-47	0.0	0.0	0.0	0.17161117036604312	0.0
48-49	0.0	0.0	0.0	0.21729916297597907	0.0
50-51	0.0	0.0	0.0	0.2664686556089465	0.0
52-53	0.0	0.0	0.0	0.31813768671998777	0.0
54-55	0.0	0.0	0.0	0.37389524862730705	0.0
56-57	0.0	0.0	0.0	0.4417934259995565	0.0
58-59	0.0	0.0	0.0	0.5186185265077841	0.0
60-61	0.0	0.0	0.0	0.6025316039859783	0.0
62-63	0.0	0.0	0.0	0.6910152661097932	0.0
64-65	0.0	0.0	0.0	0.784872935961467	0.0
66-67	0.0	0.0	0.0	0.892567336673907	0.0
68-69	0.0	0.0	0.0	1.0167408374953624	0.0
70-71	0.0	0.0	0.0	1.1519837230054308	0.0
72-73	0.0	0.0	0.0	1.3005277239881066	0.0
74-75	0.0	0.0	0.0	1.4467864326479718	0.0
76-77	0.0	0.0	0.0	1.6081852029464558	0.0
78-79	0.0	0.0	0.0	1.789651696454619	0.0
80-81	0.0	0.0	0.0	1.9972740747511977	0.0
82-83	0.0	0.0	0.0	2.214251868494282	0.0
84-85	0.0	0.0	0.0	2.431925962241972	0.0
86-87	0.0	0.0	0.0	2.667043263797363	0.0
88	0.0	0.0	0.0	2.857543803519136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2170	0.0	35.533085	1
GTATCAA	3425	0.0	33.083027	1
TCAACGC	3320	0.0	32.28833	4
ATCAACG	3480	0.0	31.074015	3
CAACGCA	3480	0.0	30.93891	5
AACGCAG	3680	0.0	28.995184	6
GTACATG	7475	0.0	26.857494	1
TATCAAC	4200	0.0	26.082872	2
TACATGG	7305	0.0	26.00219	2
ACGCAGA	4365	0.0	24.01422	7
ACATGGG	7660	0.0	23.9992	3
CGCAGAG	4415	0.0	23.227362	8
CATGGGG	5375	0.0	18.894007	4
GCAGAGT	5575	0.0	17.890375	9
GAGTACA	5285	0.0	16.724827	1
ATAACCG	225	3.8652244E-5	16.716919	5
AGAGTAC	4685	0.0	16.274105	10-11
GTGTAGG	1275	0.0	16.225245	1
GTATAGA	1405	0.0	15.727885	1
CTAGTAC	690	0.0	14.990716	3
>>END_MODULE