FastQCFastQC Report
Wed 25 May 2016
SRR1033057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800517
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.1617558472239233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.13115435471379033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37500.053.405461
GTATCAA70750.042.3602641
ATCAACG84100.034.8731163
TCAACGC84350.034.6013574
CAACGCA86200.033.8575445
AACGCAG89750.032.727796
TATCAAC89700.032.71662
ACGCAGA103350.028.2391917
CGCAGAG104950.027.5847728
GTGGTAT19250.024.234411
TATACCG1751.6489139E-724.169945
GTACATG73250.024.124131
GCAGAGT119550.024.0980599
TACATGG71500.023.7175052
ACATGGG73650.022.2717743
TGGTATC19000.022.0652542
GAGTACT82550.021.03644612-13
AGAGTAC107300.020.98006810-11
CCGGTCG1150.00396144720.4335259
CAGAGTA116900.020.14161910-11
Produced by FastQC (version 0.11.5)