FastQCFastQC Report
Wed 25 May 2016
SRR1033056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916043
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41650.035.2175181
TCAACGC40750.034.8392374
ATCAACG42650.033.397423
GGTATCA25050.033.2188571
CAACGCA43050.032.9784745
AACGCAG46000.030.7529056
TATCAAC50150.029.1526972
GTACATG87050.026.1394061
TACATGG88000.025.321242
ACGCAGA54900.024.9118297
CGCAGAG56050.023.8337888
ACATGGG90650.023.4919853
CATGGGG63050.020.578484
TAGACCG1200.005059295319.5877935
GCAGAGT67150.018.7764639
GAGTACA62200.017.8377781
TCTGTCG7400.017.1625738
AGAGTAC56250.016.81567210-11
GTATTAG13250.016.3215391
TATACTG19150.015.9566365
Produced by FastQC (version 0.11.5)