##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033056_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2916043
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49268443572334	33.0	31.0	34.0	30.0	34.0
2	31.569629460196573	33.0	31.0	34.0	29.0	34.0
3	31.727776990942864	34.0	31.0	34.0	30.0	34.0
4	35.066944143141924	37.0	35.0	37.0	33.0	37.0
5	35.1591115083008	37.0	35.0	37.0	33.0	37.0
6	35.19375503036135	37.0	35.0	37.0	33.0	37.0
7	35.096848709021096	37.0	35.0	37.0	33.0	37.0
8	35.10695349828518	37.0	35.0	37.0	33.0	37.0
9	36.71084411306692	39.0	38.0	39.0	33.0	39.0
10-11	36.69991954851146	39.0	37.5	39.0	33.0	39.0
12-13	36.631216000587095	39.0	37.0	39.0	33.0	39.0
14-15	38.000029492020516	41.0	38.0	41.0	33.0	41.0
16-17	37.99717785368735	40.5	38.0	41.0	33.0	41.0
18-19	38.00496649054901	40.0	38.5	41.0	33.0	41.0
20-21	38.02727514649132	40.0	39.0	41.0	33.0	41.0
22-23	37.99035919566344	40.0	38.0	41.0	33.0	41.0
24-25	37.9949911575378	40.0	38.0	41.0	33.0	41.0
26-27	37.90070894016309	40.0	38.0	41.0	32.5	41.0
28-29	37.90669513446818	40.0	38.0	41.0	32.5	41.0
30-31	38.05417701316476	40.0	38.0	41.0	33.0	41.0
32-33	38.04054278349119	40.0	38.0	41.0	33.0	41.0
34-35	37.99305600088887	40.0	38.0	41.0	33.0	41.0
36-37	37.921185147132604	40.0	38.0	41.0	33.0	41.0
38-39	37.832300826839656	40.0	38.0	41.0	32.0	41.0
40-41	37.76007795495471	40.0	38.0	41.0	32.0	41.0
42-43	37.64335162410157	40.0	38.0	41.0	32.0	41.0
44-45	37.516998720526416	40.0	38.0	41.0	31.5	41.0
46-47	37.38882588494066	40.0	38.0	41.0	31.0	41.0
48-49	37.27104127065341	40.0	37.0	41.0	31.0	41.0
50-51	37.1476785493218	40.0	37.0	41.0	31.0	41.0
52-53	36.99157282660098	40.0	37.0	41.0	30.5	41.0
54-55	36.82991763153012	40.0	36.0	41.0	30.0	41.0
56-57	36.65279781539573	40.0	36.0	41.0	30.0	41.0
58-59	36.4517333935062	39.5	35.5	41.0	30.0	41.0
60-61	36.2181761380062	39.0	35.0	41.0	30.0	41.0
62-63	35.964331287295835	39.0	35.0	41.0	29.5	41.0
64-65	35.68679062688719	38.5	35.0	40.5	29.0	41.0
66-67	35.385612283495135	38.0	35.0	40.0	29.0	41.0
68-69	35.06761680126116	37.0	34.5	40.0	29.0	41.0
70-71	34.735012480954495	37.0	34.0	39.5	28.0	41.0
72-73	34.385375490004776	36.0	34.0	39.0	28.0	41.0
74-75	34.033337642826254	36.0	34.0	39.0	28.0	40.5
76-77	33.67304254429719	35.0	34.0	38.5	27.5	40.0
78-79	33.29014935650812	35.0	34.0	37.0	27.0	39.0
80-81	32.939598455852675	35.0	33.0	37.0	26.5	39.0
82-83	32.71840435823478	35.0	33.0	36.5	26.5	39.0
84-85	32.490909427604464	35.0	33.0	36.0	26.5	37.0
86-87	32.3100218343831	35.0	33.0	36.0	26.5	37.0
88-89	32.085174326990376	35.0	33.0	35.0	26.0	37.0
90-91	31.875154447310962	35.0	33.0	35.0	26.0	36.0
92-93	31.652172310216276	35.0	33.0	35.0	25.0	36.0
94-95	31.445395181072435	35.0	33.0	35.0	25.0	36.0
96-97	31.264750382624673	35.0	33.0	35.0	24.5	36.0
98-99	31.08484031271144	35.0	33.0	35.0	24.0	35.0
100	30.94577514803451	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.030547982480662483
1101	2	0.07106430829609778
1101	3	0.10808324741494246
1101	4	0.003096141350972914
1101	5	-0.016515271432830048
1101	6	0.07251173093220586
1101	7	0.15370528168977415
1101	8	0.16079819027291364
1101	9	0.2219970461457379
1101	10-11	0.1895649404422315
1101	12-13	0.19085171074431884
1101	14-15	0.23513847794669118
1101	16-17	0.22572571956636978
1101	18-19	0.21031490852800516
1101	20-21	0.2352007655657289
1101	22-23	0.20928176634453166
1101	24-25	0.17708519138861334
1101	26-27	0.14116854455520667
1101	28-29	0.19444725103004146
1101	30-31	0.244189940332042
1101	32-33	0.246320486103059
1101	34-35	0.22576152870048816
1101	36-37	0.2635604485208063
1101	38-39	0.2941948176968836
1101	40-41	0.3289821988207464
1101	42-43	0.3782688117539408
1101	44-45	0.33374637503037263
1101	46-47	0.27208457248814
1101	48-49	0.3630013673987236
1101	50-51	0.268724039369431
1101	52-53	0.2508608915106407
1101	54-55	0.23541059680802334
1101	56-57	0.2641813542330169
1101	58-59	0.3143417302534388
1101	60-61	0.3369117434095017
1101	62-63	0.3381824966899103
1101	64-65	0.29089398382174636
1101	66-67	0.2083422881796153
1101	68-69	0.26837580954697415
1101	70-71	0.27796207687949703
1101	72-73	0.21707750398379488
1101	74-75	0.2806957975797104
1101	76-77	0.29213767362122667
1101	78-79	0.3159191312754288
1101	80-81	0.2713478156234217
1101	82-83	0.22667938380899244
1101	84-85	0.28165411877061075
1101	86-87	0.2982245453057004
1101	88-89	0.30148107265372914
1101	90-91	0.27766297368740567
1101	92-93	0.2562435457835548
1101	94-95	0.3000509299809231
1101	96-97	0.2401915718717902
1101	98-99	0.20840749848852624
1101	100	0.1841337457528418
1102	1	0.016055338278260223
1102	2	0.13024719642712412
1102	3	0.08809919214303008
1102	4	-0.06366703991719902
1102	5	0.03711574637191717
1102	6	0.10472448192512473
1102	7	0.10882764355748265
1102	8	0.1956541609872744
1102	9	0.2208671363674668
1102	10-11	0.21366907287077197
1102	12-13	0.16771737428672395
1102	14-15	0.29004960991739637
1102	16-17	0.23563230795019763
1102	18-19	0.18671957761060298
1102	20-21	0.20613490414165625
1102	22-23	0.18822941075161737
1102	24-25	0.14342955811358138
1102	26-27	0.07245907658840167
1102	28-29	0.14233207950800875
1102	30-31	0.1925881121384947
1102	32-33	0.20781044638633972
1102	34-35	0.21096464172107687
1102	36-37	0.22316236943820655
1102	38-39	0.3007128859310271
1102	40-41	0.2276087768515893
1102	42-43	0.25463709321165595
1102	44-45	0.2092106283915811
1102	46-47	0.18221463008192984
1102	48-49	0.19038222638732805
1102	50-51	0.3099889862248517
1102	52-53	0.28941811893996316
1102	54-55	0.3278489369188833
1102	56-57	0.3169720768455946
1102	58-59	0.23836190394433032
1102	60-61	0.20928166251678704
1102	62-63	0.27944727248987533
1102	64-65	0.27177405353687334
1102	66-67	0.24192946977905194
1102	68-69	0.21106577223682876
1102	70-71	0.13500870058096126
1102	72-73	0.17258757189517127
1102	74-75	0.14860644835371062
1102	76-77	0.1612570966381881
1102	78-79	0.09802730250029157
1102	80-81	0.23164115449974787
1102	82-83	0.15780938914237908
1102	84-85	0.2246010185555889
1102	86-87	0.22413809647246552
1102	88-89	0.22091103210885876
1102	90-91	0.25763332328819644
1102	92-93	0.23370917159938642
1102	94-95	0.24038631428886248
1102	96-97	0.23838177563187202
1102	98-99	0.26801082913884144
1102	100	0.1519304912870858
1103	1	0.2002760264924106
1103	2	0.21475332265301006
1103	3	0.22861947862004328
1103	4	0.19557812363777316
1103	5	0.18076204547938346
1103	6	0.15832115873208608
1103	7	0.31054790626951956
1103	8	0.23681509722395333
1103	9	0.3707155198469039
1103	10-11	0.3259964933750652
1103	12-13	0.322953604736945
1103	14-15	0.3725401620483666
1103	16-17	0.3658524869968929
1103	18-19	0.4286043984218324
1103	20-21	0.4167482327908729
1103	22-23	0.45624491194431016
1103	24-25	0.40336493919556915
1103	26-27	0.3265294742987024
1103	28-29	0.33746338816882115
1103	30-31	0.2994836286234346
1103	32-33	0.28683779878159754
1103	34-35	0.2848586447674677
1103	36-37	0.30541883082774035
1103	38-39	0.3539516530339881
1103	40-41	0.30936846990455535
1103	42-43	0.31163583521788496
1103	44-45	0.2684625942400558
1103	46-47	0.30645590886717855
1103	48-49	0.32599340958743994
1103	50-51	0.33407762958060516
1103	52-53	0.3840323097870595
1103	54-55	0.34171209716111406
1103	56-57	0.35076765041814184
1103	58-59	0.2954630534745277
1103	60-61	0.32628954887198347
1103	62-63	0.31088709988956964
1103	64-65	0.35825278747219613
1103	66-67	0.4025464578899616
1103	68-69	0.3521438467802014
1103	70-71	0.3942528021745133
1103	72-73	0.39355866961025754
1103	74-75	0.3837795868142564
1103	76-77	0.3887165203834755
1103	78-79	0.33649998382949065
1103	80-81	0.394857077951265
1103	82-83	0.469611425754799
1103	84-85	0.3448619223880911
1103	86-87	0.24994826541330184
1103	88-89	0.19826639798471035
1103	90-91	0.25447062993587366
1103	92-93	0.22210887895725762
1103	94-95	0.23290895774621845
1103	96-97	0.21747525665247025
1103	98-99	0.19538552558291045
1103	100	0.2202118559591888
1104	1	-0.13432651080114866
1104	2	-0.031952034703767396
1104	3	-0.010007024168373846
1104	4	-0.023619624011338658
1104	5	-0.06392893324909465
1104	6	0.031034638682186255
1104	7	-0.010143923832444557
1104	8	0.053473211153104216
1104	9	0.20407626651481792
1104	10-11	0.11774636118373394
1104	12-13	0.18558786460756238
1104	14-15	0.17853415451638455
1104	16-17	0.1478628204867931
1104	18-19	0.22150793858654794
1104	20-21	0.14667551502062537
1104	22-23	0.12467090736316777
1104	24-25	0.020511589949130382
1104	26-27	-0.044351630391822994
1104	28-29	0.0033750180531484375
1104	30-31	0.034189216653388144
1104	32-33	-0.03964044578435022
1104	34-35	0.032002810491107425
1104	36-37	0.14882703822409127
1104	38-39	0.12335140687115143
1104	40-41	0.1257191867465437
1104	42-43	0.09353492740191172
1104	44-45	0.10044228274210099
1104	46-47	0.049361761335518395
1104	48-49	0.09530908472159183
1104	50-51	0.004352548552475355
1104	52-53	0.05452985066608562
1104	54-55	0.10310885670438097
1104	56-57	0.1314877742016023
1104	58-59	0.14104485414058132
1104	60-61	0.10357684897611819
1104	62-63	0.11235393616232159
1104	64-65	0.06529180188481121
1104	66-67	0.044993359876698946
1104	68-69	0.11368382985302361
1104	70-71	0.15344955804037141
1104	72-73	0.10939560930148673
1104	74-75	0.1803053088240958
1104	76-77	0.1674010090607112
1104	78-79	0.19828991856681455
1104	80-81	0.16900694419982898
1104	82-83	0.13634247475501837
1104	84-85	0.10732668761684039
1104	86-87	0.09927766950201544
1104	88-89	0.13311963913791658
1104	90-91	0.20530902108514937
1104	92-93	0.23156140903531153
1104	94-95	0.22836695266137852
1104	96-97	0.23610164243275022
1104	98-99	0.14849847130729543
1104	100	0.17318673208378854
1105	1	-0.03203126959060043
1105	2	-0.08391753241077282
1105	3	-0.013360417510327238
1105	4	-0.08321219568658478
1105	5	-0.06858503164583851
1105	6	0.006884848450219749
1105	7	0.014724790202464533
1105	8	0.04673531251498275
1105	9	0.04181186934998493
1105	10-11	0.0901651816690503
1105	12-13	0.10075766457929802
1105	14-15	0.11050673235352804
1105	16-17	0.08843758359303422
1105	18-19	0.11850927553636126
1105	20-21	0.11205341939717783
1105	22-23	0.08689840229342849
1105	24-25	0.024510124230403108
1105	26-27	0.08834371703148491
1105	28-29	0.08453811253453125
1105	30-31	0.1546084675207453
1105	32-33	0.07240774490426816
1105	34-35	0.06114552923515504
1105	36-37	0.07834771319794243
1105	38-39	0.023566403438039174
1105	40-41	0.11058602974308229
1105	42-43	0.09154748928553147
1105	44-45	0.020063144690155355
1105	46-47	0.05334560386063458
1105	48-49	-0.013415582261671943
1105	50-51	0.02579673305845631
1105	52-53	0.03337734073078025
1105	54-55	0.0263580422841585
1105	56-57	0.007817864257745555
1105	58-59	-0.04551268590633839
1105	60-61	-0.07289328808499818
1105	62-63	-0.025983153651409907
1105	64-65	0.0016391741994112863
1105	66-67	-0.04021698934202078
1105	68-69	-0.03870009122977791
1105	70-71	-0.11544290608257057
1105	72-73	-0.06391720454575989
1105	74-75	-0.03582435975149423
1105	76-77	-0.06652658780441811
1105	78-79	-0.016213880332429653
1105	80-81	-0.060046500520066104
1105	82-83	-0.06971097169331841
1105	84-85	-0.14908091657687805
1105	86-87	-0.14527952096752728
1105	88-89	-0.10839970666410181
1105	90-91	-0.08834888266915186
1105	92-93	-0.09558008726819978
1105	94-95	-0.020953823685584183
1105	96-97	-0.08083145995193775
1105	98-99	-0.04255490816391472
1105	100	0.04257345497090981
1106	1	-0.040961596576316595
1106	2	0.10531456177771403
1106	3	0.13387295769667418
1106	4	0.12312814887213364
1106	5	0.1425316072633791
1106	6	0.18949549365667906
1106	7	0.24367471176878297
1106	8	0.2354510935026468
1106	9	0.1735278621321541
1106	10-11	0.1984947954423859
1106	12-13	0.2896211173957681
1106	14-15	0.3005487150290236
1106	16-17	0.23017163119123296
1106	18-19	0.2944719461893257
1106	20-21	0.3056534422783628
1106	22-23	0.24225434317057193
1106	24-25	0.25369711008281115
1106	26-27	0.2294138052286243
1106	28-29	0.28697317537271516
1106	30-31	0.32240451698921646
1106	32-33	0.21824031365189
1106	34-35	0.17247942667042793
1106	36-37	0.18281733093652264
1106	38-39	0.22184302552670943
1106	40-41	0.25049455419551236
1106	42-43	0.30667194982972745
1106	44-45	0.2506431971733676
1106	46-47	0.18011277074917587
1106	48-49	0.2809223845807054
1106	50-51	0.30804544645291543
1106	52-53	0.2459287439565827
1106	54-55	0.2746447210242806
1106	56-57	0.3770907755342989
1106	58-59	0.31615356284375906
1106	60-61	0.2513806186052676
1106	62-63	0.30004520815595015
1106	64-65	0.3774146947720851
1106	66-67	0.3180469640311969
1106	68-69	0.3412277192352562
1106	70-71	0.26793937953083713
1106	72-73	0.25025145892834644
1106	74-75	0.33162620632214157
1106	76-77	0.32346956073386224
1106	78-79	0.2890057729273465
1106	80-81	0.34104423097881664
1106	82-83	0.2854924433035748
1106	84-85	0.31156361841729563
1106	86-87	0.2780679077383965
1106	88-89	0.3642865151493382
1106	90-91	0.3208754091570327
1106	92-93	0.27716557232819383
1106	94-95	0.20733408625110528
1106	96-97	0.2295029385731695
1106	98-99	0.16834637385924012
1106	100	0.20600352351811324
1107	1	-0.09291841467874562
1107	2	-0.009203218555072112
1107	3	-0.049349459184863775
1107	4	0.05133374256418932
1107	5	-0.02683931516102689
1107	6	0.02695101269106459
1107	7	0.056339112384542034
1107	8	0.002645111443115411
1107	9	-0.022514272836119176
1107	10-11	0.04546025021951294
1107	12-13	0.03421934597959364
1107	14-15	0.07075137554792832
1107	16-17	0.10514608523088498
1107	18-19	0.06631542730794848
1107	20-21	0.06874559321132523
1107	22-23	0.0648502555217334
1107	24-25	-0.0018776900142114528
1107	26-27	0.03933968381532793
1107	28-29	0.03633803170774286
1107	30-31	0.07502941668719387
1107	32-33	0.010581445329322037
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2216	36-37	-0.8771364002463358
2216	38-39	-0.7673659875904733
2216	40-41	-0.704381666592333
2216	42-43	-0.8107678539779997
2216	44-45	-0.603866429991534
2216	46-47	-0.585984940148137
2216	48-49	-0.6675504042802771
2216	50-51	-0.6043333646487241
2216	52-53	-0.6413398483943951
2216	54-55	-0.6946019548785003
2216	56-57	-0.7480756726207574
2216	58-59	-0.6562597705784157
2216	60-61	-0.5865147691895629
2216	62-63	-0.6763611275175094
2216	64-65	-0.6736453919159757
2216	66-67	-0.6303094163294389
2216	68-69	-0.6606035392460612
2216	70-71	-0.726072914399154
2216	72-73	-0.6878811602282866
2216	74-75	-0.7582982971433267
2216	76-77	-0.6095982913721514
2216	78-79	-0.6008189186328678
2216	80-81	-0.677344432520961
2216	82-83	-0.9128581506055333
2216	84-85	-0.7054182578904644
2216	86-87	-0.5792703517355591
2216	88-89	-0.6434127750634744
2216	90-91	-0.6138274662008669
2216	92-93	-0.817137499558644
2216	94-95	-0.8317139188521914
2216	96-97	-0.8547536698613385
2216	98-99	-0.7557245324410999
2216	100	-0.6311204469976381
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35645.0
3	7295.0
4	4056.0
5	3424.0
6	2981.0
7	3077.0
8	3381.0
9	4150.0
10	4457.0
11	4972.0
12	5512.0
13	6189.0
14	6585.0
15	7086.0
16	6810.0
17	6769.0
18	6668.0
19	6693.0
20	6644.0
21	6925.0
22	8278.0
23	11729.0
24	17642.0
25	23613.0
26	23656.0
27	21081.0
28	22783.0
29	28317.0
30	34582.0
31	41997.0
32	54420.0
33	72490.0
34	102926.0
35	158303.0
36	284121.0
37	582693.0
38	913270.0
39	374147.0
40	676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73635403752071	20.036600384945732	13.65984289904148	23.567202678492073
2	15.186886469733166	20.37295298359299	40.56047243181415	23.879688114859693
3	17.726930381538473	26.16716734405372	30.15411484755789	25.951787426849922
4	12.05760845169221	17.1414177257936	39.548361324667674	31.25261249784651
5	12.785727917139761	38.29488617563592	35.69960005986059	13.219785847363733
6	30.154494275968542	38.651130287701484	18.26009625516474	12.934279181165234
7	26.806349428634483	32.87820422391391	23.081567305391975	17.233879042059627
8	24.806813325254925	36.641636186693624	20.81541580061206	17.73613468743939
9	24.79239523991943	17.44442934092354	21.280998260349	36.482177158808035
10-11	23.47228949494027	27.454986970928918	29.164917385288046	19.907806148842763
12-13	24.186653740551726	24.835940412624108	29.03751708739477	21.939888759429394
14-15	21.597469765423593	26.756911379481462	26.03650308975683	25.609115765338114
16-17	21.396775444508886	29.936768374921495	26.602823030382066	22.06363315018755
18-19	21.370912031102264	28.969028691020977	28.347759195682727	21.31230008219403
20-21	22.642158353487293	28.23743069108803	27.75897008518552	21.36144087023916
22-23	22.25522347473337	28.04774964035936	27.759608216877247	21.937418668030027
24-25	22.05479496692644	28.095038906385085	27.776711598820814	22.073454527867657
26-27	21.837742326263417	28.047283039274497	27.870844301703883	22.244130332758203
28-29	21.89711876539499	28.07034972742852	27.93507630834653	22.097455198829962
30-31	22.096070918067053	28.453472030366	27.60330635239045	21.847150699176503
32-33	21.82410607899231	28.1032250890901	28.086649388586583	21.98601944333101
34-35	21.942189715325313	27.932365043679024	28.023758534589664	22.101686706406
36-37	22.249360481962682	28.245690211990087	27.533046411570837	21.971902894476393
38-39	22.018932836255008	28.401073296574108	27.698371883157762	21.88162198401312
40-41	21.806248100548125	28.10052753066194	27.98018100542975	22.113043363360187
42-43	21.819861883643533	28.031768992714955	27.930922946379315	22.217446177262193
44-45	21.922086452554325	28.18781696970612	27.86265383905266	22.027442738686897
46-47	21.950305003819842	28.335410850818914	27.771821312446505	21.942462832914742
48-49	21.900396180414468	28.140306346574125	27.83117648835345	22.12812098465796
50-51	21.902735042852367	28.05431650010055	27.83197286989037	22.21097558715671
52-53	21.948520024490087	28.00695098413361	27.85302996191956	22.191499029456743
54-55	22.041531290021044	28.4027065720643	27.643533560368034	21.912228577546625
56-57	21.842601275037254	28.14798736802009	27.737456600089395	22.27195475685326
58-59	21.75524391894825	27.81365585108473	28.096194606009682	22.334905623957336
60-61	22.02023955851944	28.205311657357058	27.66413286143613	22.11031592268737
62-63	21.858392867070183	28.15888558609817	27.74842366286735	22.23429788396429
64-65	21.870039250190278	27.970873599837482	27.842979335828304	22.316107814143933
66-67	21.73467155445095	27.970062464235983	27.78130568355057	22.51396029776249
68-69	22.0880673775354	28.181605422212858	27.63247974408451	22.097847456167234
70-71	22.01213739564815	27.9439583927387	27.784194415192125	22.259709796421028
72-73	21.79525649940492	27.99839208022305	27.935787521259215	22.270563899112812
74-75	21.85405728048517	28.09118636257029	27.74614324976705	22.308613107177482
76-77	21.87020863645932	28.031135488716725	27.691222638027728	22.40743323679623
78-79	21.93065268615121	28.141888965039847	27.76956510177876	22.157893247030184
80-81	21.9032294310434	27.92310641571838	27.844401310265027	22.329262842973193
82-83	21.785262171169844	27.975303995104444	27.980810981857996	22.258622851867717
84-85	21.762289703794874	27.947920731785764	27.82461843273309	22.465171131686272
86-87	21.94648553367288	28.017457573971523	27.767666676501406	22.268390215854193
88-89	21.72277075884575	27.9480357921219	27.80136926497427	22.527824184058087
90-91	21.682512378729957	27.857931667146392	27.913388514583282	22.546167439540373
92-93	21.888218025351165	27.95962834030404	27.767403579710148	22.384750054634647
94-95	21.673726796559333	27.75280311028812	27.907854369489947	22.6656157236626
96-97	21.69630409696597	27.714461029222186	27.967484053974356	22.621750819837487
98-99	21.655015589662515	27.854102167182663	27.897639318885446	22.593242924269372
100	21.744348705047745	27.66829678686873	27.802487485885273	22.78486702219825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	572.0
1	515.5
2	430.0
3	393.0
4	393.0
5	382.5
6	360.5
7	356.5
8	322.5
9	292.5
10	286.0
11	285.5
12	298.5
13	302.5
14	308.5
15	348.0
16	441.5
17	611.0
18	888.5
19	1233.5
20	1734.5
21	2505.5
22	3734.5
23	5891.0
24	8651.5
25	12069.0
26	16637.5
27	22490.0
28	30224.5
29	38712.5
30	46327.0
31	54568.0
32	63104.0
33	71533.5
34	79898.0
35	87150.0
36	93251.0
37	98752.5
38	102669.0
39	103892.5
40	105624.5
41	107407.5
42	106888.0
43	105864.5
44	103690.0
45	100760.0
46	99671.5
47	97922.5
48	95683.0
49	93167.5
50	90350.5
51	88025.0
52	82612.0
53	78092.0
54	75765.5
55	71866.0
56	65915.0
57	59633.0
58	54199.5
59	49204.5
60	43999.0
61	37580.5
62	31984.0
63	26859.0
64	21633.5
65	17279.5
66	13482.5
67	10215.5
68	7639.0
69	5725.5
70	4224.0
71	3016.0
72	2298.5
73	1706.0
74	1195.0
75	802.0
76	512.0
77	352.5
78	239.5
79	134.5
80	69.0
81	47.5
82	42.5
83	28.5
84	17.5
85	16.0
86	13.5
87	8.0
88	4.5
89	4.0
90	3.0
91	4.5
92	5.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	2.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06399082592403472
2	0.04293489499297507
3	0.05685787212328487
4	0.07424444701261264
5	0.08940197383920608
6	0.08700146054087679
7	0.08861323375546931
8	0.10041004196440176
9	0.09653492763995593
10-11	0.10089014462406762
12-13	0.10551970598513122
14-15	0.10670281611073636
16-17	0.07383293044718477
18-19	0.09651778111639643
20-21	0.09043076525277577
22-23	0.0574580004478672
24-25	0.022204748009545812
26-27	0.1852167474896632
28-29	0.06669997664643491
30-31	0.07743370039467867
32-33	0.0733528277875189
34-35	0.02151888706716602
36-37	0.052931318228160565
38-39	0.0760105389392406
40-41	0.08139454733692199
42-43	0.07529038494974183
44-45	0.0727355529393771
46-47	0.07918264579774716
48-49	0.07609627155703809
50-51	0.07150100324309347
52-53	0.07582192718008617
54-55	0.06690573492914885
56-57	0.06894617123272874
58-59	0.06616843441609056
60-61	0.0634078441230119
62-63	0.06889473166205025
64-65	0.0657397713271032
66-67	0.06774591458356409
68-69	0.06707720016474379
70-71	0.05980707417551799
72-73	0.058932601473983756
74-75	0.06068154687705222
76-77	0.05553758980920378
78-79	0.051799647673233896
80-81	0.0468271558409804
82-83	0.05361717917054035
84-85	0.043054920657891536
86-87	0.04132312177838256
88-89	0.04346643722331941
90-91	0.03991710684650398
92-93	0.04204327576788134
94-95	0.0411688030663471
96-97	0.04418659121281819
98-99	0.04427232383061567
100	0.052605534280530164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2916043.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.60281241994993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92427987325904	53.23181820198252
2	12.402528908713887	16.520866128801543
3	3.5416225067535083	7.076460584387302
4	1.4705869409025194	3.91780904688634
5	0.7225551517943818	2.406210261901483
6	0.45924398786812337	1.835216470738223
7	0.30480764139535577	1.421073231481557
8	0.19968960641199066	1.0639911518457155
9	0.14763839816061297	0.8849819284805867
>10	0.7823462292670629	9.170983770273725
>50	0.036651343567347686	1.6444871430807757
>100	0.007946461785499908	0.7715222102196193
>500	1.0295012070611349E-4	0.05457986992059814
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.858609423797934E-5	0.0
10-11	0.0	0.0	0.0	6.858609423797934E-5	0.0
12-13	0.0	0.0	0.0	1.2002566491646385E-4	0.0
14-15	0.0	0.0	1.7146523559494835E-5	2.400513298329277E-4	0.0
16-17	0.0	0.0	3.429304711898967E-5	3.2578394763040184E-4	0.0
18-19	0.0	0.0	3.429304711898967E-5	3.429304711898967E-4	0.0
20-21	0.0	0.0	3.429304711898967E-5	3.7722351830888636E-4	0.0
22-23	0.0	0.0	3.429304711898967E-5	4.286630889873709E-4	0.0
24-25	0.0	0.0	3.429304711898967E-5	5.486887539038348E-4	0.0
26-27	0.0	0.0	3.429304711898967E-5	6.858609423797935E-4	0.0
28-29	0.0	0.0	3.429304711898967E-5	0.0010459379371291848	0.0
30-31	0.0	0.0	3.429304711898967E-5	0.0029663485757926065	0.0
32-33	0.0	0.0	3.429304711898967E-5	0.008281770879236006	0.0
34-35	0.0	0.0	3.429304711898967E-5	0.015586189915580806	0.0
36-37	0.0	0.0	3.429304711898967E-5	0.030932328501328682	0.0
38-39	0.0	0.0	3.429304711898967E-5	0.056086278563107606	0.0
40-41	0.0	0.0	3.429304711898967E-5	0.08626416002781852	0.0
42-43	0.0	0.0	3.429304711898967E-5	0.11834530560763336	0.0
44-45	0.0	0.0	3.429304711898967E-5	0.15558755477885616	0.0
46-47	0.0	0.0	3.429304711898967E-5	0.20135162615914787	0.0
48-49	0.0	0.0	3.429304711898967E-5	0.2519510171832171	0.0
50-51	0.0	0.0	3.429304711898967E-5	0.30935757806040587	0.0
52-53	0.0	0.0	3.429304711898967E-5	0.36871884262337695	0.0
54-55	0.0	0.0	3.429304711898967E-5	0.43252105678825725	0.0
56-57	0.0	0.0	3.429304711898967E-5	0.5102633946070068	3.429304711898967E-5
58-59	0.0	0.0	3.429304711898967E-5	0.5993567310221419	3.429304711898967E-5
60-61	0.0	0.0	3.429304711898967E-5	0.6976234575416069	3.429304711898967E-5
62-63	0.0	0.0	3.429304711898967E-5	0.7978963273175327	3.429304711898967E-5
64-65	0.0	0.0	3.429304711898967E-5	0.903484619396902	3.429304711898967E-5
66-67	0.0	0.0	3.429304711898967E-5	1.022275734617082	3.429304711898967E-5
68-69	0.0	0.0	3.429304711898967E-5	1.1613683337317042	3.429304711898967E-5
70-71	0.0	0.0	3.429304711898967E-5	1.3150011848247778	3.429304711898967E-5
72-73	0.0	0.0	3.429304711898967E-5	1.4788876570064295	3.429304711898967E-5
74-75	0.0	0.0	3.429304711898967E-5	1.6483810423920362	3.429304711898967E-5
76-77	0.0	0.0	3.429304711898967E-5	1.8256932425207721	3.429304711898967E-5
78-79	0.0	0.0	3.429304711898967E-5	2.0318458952765788	3.429304711898967E-5
80-81	0.0	0.0	3.429304711898967E-5	2.2636497472773893	3.429304711898967E-5
82-83	0.0	0.0	3.429304711898967E-5	2.49953447188536	3.429304711898967E-5
84-85	0.0	0.0	3.429304711898967E-5	2.742346392011366	3.429304711898967E-5
86-87	0.0	0.0	3.429304711898967E-5	3.0020304913199154	3.429304711898967E-5
88	0.0	0.0	3.429304711898967E-5	3.2115438626933828	3.429304711898967E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4165	0.0	35.217518	1
TCAACGC	4075	0.0	34.839237	4
ATCAACG	4265	0.0	33.39742	3
GGTATCA	2505	0.0	33.218857	1
CAACGCA	4305	0.0	32.978474	5
AACGCAG	4600	0.0	30.752905	6
TATCAAC	5015	0.0	29.152697	2
GTACATG	8705	0.0	26.139406	1
TACATGG	8800	0.0	25.32124	2
ACGCAGA	5490	0.0	24.911829	7
CGCAGAG	5605	0.0	23.833788	8
ACATGGG	9065	0.0	23.491985	3
CATGGGG	6305	0.0	20.57848	4
TAGACCG	120	0.0050592953	19.587793	5
GCAGAGT	6715	0.0	18.776463	9
GAGTACA	6220	0.0	17.837778	1
TCTGTCG	740	0.0	17.162573	8
AGAGTAC	5625	0.0	16.815672	10-11
GTATTAG	1325	0.0	16.321539	1
TATACTG	1915	0.0	15.956636	5
>>END_MODULE