FastQCFastQC Report
Wed 25 May 2016
SRR1033056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916043
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56380.19334419965686378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.14992920200422286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.054.457611
GTATCAA79950.045.341541
TCAACGC95750.037.0581674
ATCAACG96250.036.9658473
CAACGCA98400.036.0123985
AACGCAG102750.034.716486
TATCAAC104950.034.120112
ACGCAGA119550.029.5233827
CGCAGAG123200.028.5342628
CTATCGG1458.661973E-725.9297149
GCAGAGT137350.025.3893179
GTACATG85800.025.2181841
TATACCG1501.1640732E-625.065395
TACATGG88300.023.585272
AGAGTAC124550.021.65927510-11
ACATGGG90750.021.6488693
GAGTACT88400.021.5579212-13
CAGAGTA132150.020.75149710-11
CGTATAC1605.4157823E-520.5628643
GTGGTAT19750.020.0493621
Produced by FastQC (version 0.11.5)