##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033056_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2916043
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69302784629719	34.0	31.0	34.0	31.0	34.0
2	32.68506157145145	34.0	31.0	34.0	31.0	34.0
3	32.79016427398361	34.0	31.0	34.0	31.0	34.0
4	36.29275494222822	37.0	37.0	37.0	35.0	37.0
5	36.27958744092594	37.0	37.0	37.0	35.0	37.0
6	36.34712691136585	37.0	37.0	37.0	35.0	37.0
7	36.294535780165106	37.0	37.0	37.0	35.0	37.0
8	36.29855149598274	37.0	37.0	37.0	35.0	37.0
9	38.098113093668374	39.0	39.0	39.0	35.0	39.0
10-11	38.0559494493051	39.0	39.0	39.0	35.0	39.0
12-13	38.0314558118656	39.0	38.0	39.0	35.0	39.0
14-15	39.506030775266346	41.0	39.0	41.0	37.0	41.0
16-17	39.50431320800139	41.0	39.0	41.0	36.5	41.0
18-19	39.52744866930975	41.0	39.5	41.0	36.5	41.0
20-21	39.52274949306303	41.0	39.5	41.0	37.0	41.0
22-23	39.45374879588538	41.0	39.0	41.0	36.0	41.0
24-25	39.39920193220745	41.0	39.0	41.0	36.0	41.0
26-27	39.28884742097424	41.0	39.0	41.0	36.0	41.0
28-29	39.2347095018832	41.0	39.0	41.0	36.0	41.0
30-31	39.22963293065294	41.0	39.0	41.0	36.0	41.0
32-33	39.18506997324799	41.0	39.0	41.0	35.0	41.0
34-35	39.10508332696054	41.0	39.0	41.0	35.0	41.0
36-37	39.01769847015287	41.0	39.0	41.0	35.0	41.0
38-39	38.925552195217975	41.0	39.0	41.0	35.0	41.0
40-41	38.82337434667458	41.0	39.0	41.0	35.0	41.0
42-43	38.70211035982666	41.0	38.5	41.0	35.0	41.0
44-45	38.52830736720961	41.0	38.0	41.0	35.0	41.0
46-47	38.31943767633056	40.5	38.0	41.0	34.5	41.0
48-49	38.10740136548055	40.0	38.0	41.0	34.0	41.0
50-51	37.96124508452036	40.0	38.0	41.0	34.0	41.0
52-53	37.826454548166815	40.0	38.0	41.0	33.0	41.0
54-55	37.67353019142722	40.0	37.0	41.0	33.0	41.0
56-57	37.517807522042716	40.0	37.0	41.0	33.0	41.0
58-59	37.33558627221889	40.0	36.5	41.0	33.0	41.0
60-61	37.140888183061776	40.0	36.0	41.0	33.0	41.0
62-63	36.909174350309655	39.0	35.5	41.0	32.0	41.0
64-65	36.644421567171676	39.0	35.0	41.0	32.0	41.0
66-67	36.36393479108504	38.5	35.0	41.0	32.0	41.0
68-69	36.0566701176903	37.5	35.0	40.0	31.0	41.0
70-71	35.73102814327498	37.0	35.0	40.0	31.0	41.0
72-73	35.38639845160034	37.0	35.0	39.0	31.0	41.0
74-75	35.042835788086805	36.0	35.0	39.0	31.0	41.0
76-77	34.69001228719878	36.0	35.0	39.0	30.5	40.5
78-79	34.336231152969965	35.0	34.5	37.5	30.0	39.5
80-81	34.008862695097434	35.0	34.0	37.0	30.0	39.0
82-83	33.70743161194811	35.0	34.0	36.5	30.0	39.0
84-85	33.486009122636396	35.0	34.0	36.0	30.0	37.5
86-87	33.27560207445501	35.0	34.0	36.0	30.0	37.0
88-89	33.06813856997307	35.0	34.0	35.5	30.0	37.0
90-91	32.88697817556188	35.0	34.0	35.0	29.0	36.0
92-93	32.71093207473278	35.0	34.0	35.0	29.0	36.0
94-95	32.55573614655202	35.0	34.0	35.0	29.0	36.0
96-97	32.41086534046309	35.0	34.0	35.0	29.0	36.0
98-99	32.287329782174	35.0	34.0	35.0	29.0	35.5
100	32.1828868778684	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07379383406752282
1101	2	0.04220204511506864
1101	3	0.0558642801218312
1101	4	0.1132812451124181
1101	5	0.06923716843433425
1101	6	0.0859982126954435
1101	7	0.1372486727485125
1101	8	0.11712071829688853
1101	9	0.17097838249745223
1101	10-11	0.19992736593022187
1101	12-13	0.18937474859761494
1101	14-15	0.26818229936622373
1101	16-17	0.2338386309645628
1101	18-19	0.24346581552502755
1101	20-21	0.25775659240383675
1101	22-23	0.20581149261480647
1101	24-25	0.24504503293724156
1101	26-27	0.26102822415108307
1101	28-29	0.3086795855722144
1101	30-31	0.26139675459065614
1101	32-33	0.2565252568682439
1101	34-35	0.2926966885784523
1101	36-37	0.2748129034998996
1101	38-39	0.24226281630964763
1101	40-41	0.20690713854423137
1101	42-43	0.25789358642757776
1101	44-45	0.2815119772294423
1101	46-47	0.26456959769338084
1101	48-49	0.25797118528574714
1101	50-51	0.19893890092158273
1101	52-53	0.2381051411626487
1101	54-55	0.22191742271669312
1101	56-57	0.15196199067132454
1101	58-59	0.12588234438077706
1101	60-61	0.15548722004918858
1101	62-63	0.17303102971506945
1101	64-65	0.13919513507072168
1101	66-67	0.12800688471439514
1101	68-69	0.07361574103193647
1101	70-71	0.1391914813531372
1101	72-73	0.09645427316655741
1101	74-75	0.08142550887381361
1101	76-77	0.14685945666649758
1101	78-79	0.10876313235361579
1101	80-81	0.12561409879977248
1101	82-83	0.16259812222645564
1101	84-85	0.16468145527876032
1101	86-87	0.23104940782441474
1101	88-89	0.2714232785460098
1101	90-91	0.24946714322432229
1101	92-93	0.24665478132639862
1101	94-95	0.23091457710729912
1101	96-97	0.2808174527033813
1101	98-99	0.28321353289872775
1101	100	0.2880272050386026
1102	1	0.11417424725021164
1102	2	0.06191453510095357
1102	3	0.0952122261527748
1102	4	0.09661961770022742
1102	5	0.1151767070541112
1102	6	0.1233662802748583
1102	7	0.1351415054422347
1102	8	0.18073695101158904
1102	9	0.19499940556717377
1102	10-11	0.23080449821024018
1102	12-13	0.21593175731187841
1102	14-15	0.2648809880078957
1102	16-17	0.28282277978611603
1102	18-19	0.2611722378788883
1102	20-21	0.2594933656584999
1102	22-23	0.2561166909429957
1102	24-25	0.3076987837764449
1102	26-27	0.31373627978964436
1102	28-29	0.30883008488309827
1102	30-31	0.25720946443589554
1102	32-33	0.2566288928820484
1102	34-35	0.28346680148567316
1102	36-37	0.19401650939614257
1102	38-39	0.19673555083642214
1102	40-41	0.21859408983223716
1102	42-43	0.17141694192694246
1102	44-45	0.25279105798448853
1102	46-47	0.22807730456755593
1102	48-49	0.2041877806616057
1102	50-51	0.18294447827327787
1102	52-53	0.23470432271536623
1102	54-55	0.17109961836803933
1102	56-57	0.1593914895743893
1102	58-59	0.10411037617066654
1102	60-61	0.1476658566852791
1102	62-63	0.14778872965854362
1102	64-65	0.14098127846970243
1102	66-67	0.12413103346280963
1102	68-69	0.11021485534217135
1102	70-71	0.06199359272014249
1102	72-73	0.13902251515175124
1102	74-75	0.1521463825826217
1102	76-77	0.08847191743138438
1102	78-79	0.0691410552047671
1102	80-81	0.08902639801753054
1102	82-83	0.07222740022024254
1102	84-85	0.1546953850915216
1102	86-87	0.13270962491385063
1102	88-89	0.17244519334553132
1102	90-91	0.07821868729475057
1102	92-93	0.17208604457803034
1102	94-95	0.18545520295556628
1102	96-97	0.1522886523318263
1102	98-99	0.12813527384692236
1102	100	0.15239621153552463
1103	1	0.08497375208916935
1103	2	0.0810824223057196
1103	3	0.08430060114800142
1103	4	0.07107818240481834
1103	5	0.06696492094692275
1103	6	0.08376327015952967
1103	7	0.08126498744729815
1103	8	0.1015436743181155
1103	9	0.14374472683777384
1103	10-11	0.17405215995773204
1103	12-13	0.1350709428563519
1103	14-15	0.20214848662088514
1103	16-17	0.2565342115713136
1103	18-19	0.2293144569018324
1103	20-21	0.2135146261263614
1103	22-23	0.2352607442890573
1103	24-25	0.2161208267663639
1103	26-27	0.1602299271076646
1103	28-29	0.2451337817430499
1103	30-31	0.22779872961311298
1103	32-33	0.19204280957089281
1103	34-35	0.23514015133394395
1103	36-37	0.2480325174601603
1103	38-39	0.2478032956522469
1103	40-41	0.3205806178469288
1103	42-43	0.31767546524373813
1103	44-45	0.3491582314363697
1103	46-47	0.3423183508693626
1103	48-49	0.27323992842119793
1103	50-51	0.25822517333564576
1103	52-53	0.23739507466729748
1103	54-55	0.3497528159195511
1103	56-57	0.3027811958387332
1103	58-59	0.30137585418677304
1103	60-61	0.3082763869226284
1103	62-63	0.28968517464219445
1103	64-65	0.3518318837446657
1103	66-67	0.39726693955556414
1103	68-69	0.3513285773476511
1103	70-71	0.2762889999665674
1103	72-73	0.3084552182253262
1103	74-75	0.3306483223843344
1103	76-77	0.2327189135873695
1103	78-79	0.3396883298529616
1103	80-81	0.28507518395164766
1103	82-83	0.266055452591587
1103	84-85	0.25011531764835837
1103	86-87	0.2883215887991213
1103	88-89	0.2629884555016133
1103	90-91	0.32465946615448615
1103	92-93	0.3038945735060068
1103	94-95	0.25514182961786247
1103	96-97	0.36045869474425984
1103	98-99	0.30455020112121645
1103	100	0.3143417708002971
1104	1	0.06056780517854321
1104	2	0.05573701150408539
1104	3	0.08598980859618166
1104	4	0.08516300884553374
1104	5	0.04645216901030125
1104	6	0.07936922464782725
1104	7	0.07196289640047837
1104	8	0.08781299250056662
1104	9	0.12755731992192665
1104	10-11	0.15666890203892336
1104	12-13	0.14899594175591346
1104	14-15	0.21392758417614743
1104	16-17	0.21810302879178778
1104	18-19	0.20450825859087018
1104	20-21	0.19131453329989512
1104	22-23	0.15634616747126273
1104	24-25	0.1809484598533686
1104	26-27	0.17745433255311127
1104	28-29	0.1835150689105447
1104	30-31	0.17108669722080805
1104	32-33	0.16474219282657998
1104	34-35	0.17394525868333943
1104	36-37	0.20143062872597994
1104	38-39	0.1614728546485864
1104	40-41	0.14877473100047212
1104	42-43	0.1675918880041607
1104	44-45	0.2469110344776908
1104	46-47	0.2425629535905287
1104	48-49	0.20106697033853038
1104	50-51	0.23083429317259174
1104	52-53	0.23168329467824833
1104	54-55	0.1815101611052583
1104	56-57	0.20136542525936818
1104	58-59	0.20352796810589524
1104	60-61	0.26106981686010045
1104	62-63	0.25752532147068763
1104	64-65	0.19382560207933608
1104	66-67	0.1360869515126879
1104	68-69	0.16994830480783918
1104	70-71	0.14197043027401435
1104	72-73	0.16073016282754793
1104	74-75	0.17121582298469917
1104	76-77	0.16789955464708584
1104	78-79	0.11117411587183312
1104	80-81	0.16701361315643481
1104	82-83	0.13984518451481165
1104	84-85	0.0999759386718253
1104	86-87	0.10159761043336601
1104	88-89	0.10544786621521496
1104	90-91	0.1230023523662993
1104	92-93	0.11926615512725647
1104	94-95	0.1695701585132383
1104	96-97	0.156526274185822
1104	98-99	0.1970804296158164
1104	100	0.23065654942926272
1105	1	0.05555402733604353
1105	2	0.034204622447369104
1105	3	0.07902483590340381
1105	4	0.04541781142938106
1105	5	0.036500988851770444
1105	6	0.05412435844434782
1105	7	0.08738967247175822
1105	8	0.08083980970875615
1105	9	0.11090335010138119
1105	10-11	0.11516712152771191
1105	12-13	0.1188936136743095
1105	14-15	0.16766146159417872
1105	16-17	0.17522114143694267
1105	18-19	0.15618755872746704
1105	20-21	0.19509009857602422
1105	22-23	0.19641674407515808
1105	24-25	0.12209414892481618
1105	26-27	0.16118423887587596
1105	28-29	0.17254740287317816
1105	30-31	0.16458699948208277
1105	32-33	0.150116441548505
1105	34-35	0.1488694016894314
1105	36-37	0.17388293777312214
1105	38-39	0.16614780386480277
1105	40-41	0.11789946530655016
1105	42-43	0.06899427014278814
1105	44-45	0.04324094736853823
1105	46-47	0.07450541332755023
1105	48-49	0.13594581520352023
1105	50-51	0.1619080272077298
1105	52-53	0.17363595006193577
1105	54-55	0.1361466092781427
1105	56-57	0.11274637255787923
1105	58-59	0.12750747440239252
1105	60-61	0.14329681054221055
1105	62-63	0.09381551476666772
1105	64-65	0.18752661965629613
1105	66-67	0.12072769270314154
1105	68-69	0.10498335760957644
1105	70-71	0.12545522192979064
1105	72-73	0.06711855971065006
1105	74-75	0.03681979675904756
1105	76-77	0.07478952376415293
1105	78-79	0.11930205989450826
1105	80-81	0.09008887426999479
1105	82-83	0.04397450783412182
1105	84-85	0.07409462775235198
1105	86-87	0.05430646779067416
1105	88-89	0.057893136182720184
1105	90-91	0.08263561804554342
1105	92-93	0.18372353455871604
1105	94-95	0.07969663508875158
1105	96-97	0.09054424695681718
1105	98-99	0.09073825775078603
1105	100	0.10390493560466041
1106	1	-0.005859103390783105
1106	2	-0.008563876306773466
1106	3	0.04192184041546909
1106	4	0.0707708757556631
1106	5	0.056923628598738674
1106	6	0.06318144434455775
1106	7	0.11869374235041619
1106	8	0.1087917649534802
1106	9	0.108506668611831
1106	10-11	0.10896815954637162
1106	12-13	0.09066410100056999
1106	14-15	0.1256446232126649
1106	16-17	0.1491590370523781
1106	18-19	0.1391576549002167
1106	20-21	0.14570573786843255
1106	22-23	0.15290153126267825
1106	24-25	0.14046665672564274
1106	26-27	0.2080315504079735
1106	28-29	0.13410339238309632
1106	30-31	0.16003700541765653
1106	32-33	0.142248213821631
1106	34-35	0.14681545108614813
1106	36-37	0.12624301141118366
1106	38-39	0.13454149624280376
1106	40-41	0.2076033097357879
1106	42-43	0.1976221994628773
1106	44-45	0.16818192476844018
1106	46-47	0.15189844530852525
1106	48-49	0.14906213365308218
1106	50-51	0.1678713344744196
1106	52-53	0.1836079247064646
1106	54-55	0.15648075356781987
1106	56-57	0.09875348007834361
1106	58-59	0.10094891895990088
1106	60-61	0.06203522258362426
1106	62-63	0.13668104329239839
1106	64-65	0.1238757558401744
1106	66-67	0.07959265658018921
1106	68-69	0.1008930632316023
1106	70-71	0.1547801253767105
1106	72-73	0.1395558460836881
1106	74-75	0.14860098965218782
1106	76-77	0.14237665424371926
1106	78-79	0.1420000298451214
1106	80-81	0.16475561570374708
1106	82-83	0.14914819368812005
1106	84-85	0.11982509739694791
1106	86-87	0.11494874234484342
1106	88-89	0.09082323640683398
1106	90-91	0.08788278613965161
1106	92-93	0.08635551708864142
1106	94-95	0.14269784024669718
1106	96-97	0.14756389730518293
1106	98-99	0.2121027167449796
1106	100	0.1146682180218832
1107	1	-0.019548884662263788
1107	2	-0.01895371479529473
1107	3	-0.016573759578825786
1107	4	-0.012234332815971527
1107	5	-0.009061254883640402
1107	6	0.001409959294043972
1107	7	0.01776267388724051
1107	8	-0.01919430813182288
1107	9	-0.0046082855038349635
1107	10-11	0.025365665020849804
1107	12-13	-0.007194146347302421
1107	14-15	0.053557440766041964
1107	16-17	0.013833905977129746
1107	18-19	0.024312610767324827
1107	20-21	0.014555138958954217
1107	22-23	0.06030179132227431
1107	24-25	0.04642453553275061
1107	26-27	0.019242598660689225
1107	28-29	0.06796693046293001
1107	30-31	0.10075089508961099
1107	32-33	0.10072396250152593
1107	34-35	0.03858810212464192
1107	36-37	0.049489392543627275
1107	38-39	-0.011534609809956464
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2216	22-23	-0.22574494144797796
2216	24-25	-0.2431602849576251
2216	26-27	-0.2805428599394588
2216	28-29	-0.24167245240814594
2216	30-31	-0.2310689248710318
2216	32-33	-0.21746260037242848
2216	34-35	-0.2819744817857526
2216	36-37	-0.27156110913848863
2216	38-39	-0.2596897535062226
2216	40-41	-0.22925418591954383
2216	42-43	-0.23126993880104862
2216	44-45	-0.23744626731438956
2216	46-47	-0.15427662343189041
2216	48-49	-0.2613122637888168
2216	50-51	-0.2659925711664428
2216	52-53	-0.2674250621002514
2216	54-55	-0.27629611016492817
2216	56-57	-0.26202236997330175
2216	58-59	-0.22763876427992358
2216	60-61	-0.2689647753122131
2216	62-63	-0.26236115870140253
2216	64-65	-0.2916330652545014
2216	66-67	-0.31733113427501536
2216	68-69	-0.2848974633652901
2216	70-71	-0.29101215556708127
2216	72-73	-0.3110466637984288
2216	74-75	-0.38622656620153606
2216	76-77	-0.30392235765830833
2216	78-79	-0.40201958920647485
2216	80-81	-0.2890363029731162
2216	82-83	-0.23430585583724906
2216	84-85	-0.362300882595612
2216	86-87	-0.3422953864671783
2216	88-89	-0.5402474307201786
2216	90-91	-0.4405915527889448
2216	92-93	-0.3853445485086766
2216	94-95	-0.3153893789372475
2216	96-97	-0.3394349543472046
2216	98-99	-0.4245765781215489
2216	100	-0.4348907666978512
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14.0
3	8.0
4	9.0
5	27.0
6	76.0
7	188.0
8	401.0
9	709.0
10	1297.0
11	1993.0
12	3244.0
13	5036.0
14	5724.0
15	7530.0
16	9556.0
17	13831.0
18	16355.0
19	8592.0
20	5790.0
21	5488.0
22	5767.0
23	6126.0
24	7129.0
25	8706.0
26	10831.0
27	13942.0
28	18127.0
29	23578.0
30	30204.0
31	39179.0
32	49929.0
33	65268.0
34	91103.0
35	139387.0
36	253937.0
37	570185.0
38	1011869.0
39	483174.0
40	1734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.619290475597424	20.114261678594083	13.954512845690125	23.311935000118364
2	15.58605943099157	20.636903043053778	40.0008250484716	23.776212477483053
3	17.815828244086322	26.17580810658063	30.529857830307222	25.478505819025827
4	12.211366333102422	17.363699339501643	39.184802364899625	31.240131962496314
5	12.486728577111425	37.91161986804022	35.78750634422024	13.814145210628112
6	30.015054663173345	38.71737013209695	17.916352313411338	13.351222891318363
7	26.43537835347421	33.001811015818355	23.186215018091296	17.37659561261614
8	25.194561936435672	35.83931347945397	20.672947334705515	18.29317724940484
9	25.21413435947275	16.359566714208263	21.528832050830527	36.89746687548846
10-11	23.708413436642882	26.78916443131851	29.1390063051564	20.363415826882203
12-13	24.816797969028578	24.228809383126382	29.366593702493414	21.58779894535163
14-15	21.954141122545597	25.88609250992701	26.939287038246825	25.220479329280565
16-17	21.011958171348503	29.357129764531653	27.38736906684563	22.24354299727421
18-19	20.903639382372717	27.814678207240974	30.16851161193273	21.11317079845358
20-21	22.122997178539062	27.124416053606897	29.773708716522478	20.978878051331556
22-23	21.682464068975772	27.00939991018637	29.833779629452767	21.474356391385093
24-25	21.52376912273133	27.086733039563278	29.80079386159017	21.588703976115223
26-27	21.26117333498855	27.084638971673385	29.89962238152891	21.754565311809156
28-29	21.320108290936986	27.156803925606475	29.89939930388317	21.623688479573367
30-31	21.592601337327107	27.48375751297007	29.523955514335277	21.399685635367547
32-33	21.29526210690309	27.206474664468256	29.973546343452412	21.524716885176247
34-35	21.409475100332884	27.036535469470103	29.910344257612113	21.643645172584904
36-37	21.75482323134467	27.40449986505686	29.320040891029386	21.52063601256909
38-39	21.631814071328854	27.51783838578512	29.401881247978856	21.448466294907174
40-41	21.461234348002247	27.297073789930632	29.53826274420306	21.70342911786406
42-43	21.52325943067369	27.23504077271837	29.413592323569986	21.82810747303795
44-45	21.65708804705555	27.475675084352325	29.174398319915035	21.69283854867709
46-47	21.772827081082138	27.69357310574638	28.827644173971372	21.70595563920011
48-49	21.798649745562738	27.670168101087672	28.56929750350046	21.96188464984913
50-51	21.84124171008452	27.6991114328561	28.420619997716084	22.039026859343295
52-53	21.87750660741285	27.65607365872177	28.43867528702423	22.027744446841147
54-55	21.950790849106134	28.002364848529325	28.247011446676197	21.799832855688344
56-57	21.707327361084864	27.71814407400714	28.40009560901537	22.174432955892627
58-59	21.655493420364515	27.33798850016958	28.73904808673946	22.267469992726443
60-61	21.87623776466945	27.75993015192163	28.387252862869307	21.976579220539616
62-63	21.673977372761648	27.6610975901247	28.488211593587607	22.17671344352604
64-65	21.70511545954569	27.45801416508604	28.616844813330943	22.220025562037325
66-67	21.612901455842728	27.5755878771335	28.42036279986269	22.39114786716108
68-69	21.974583998762704	27.684490971822374	28.34701685883958	21.993908170575338
70-71	21.912976890075125	27.51348017509353	28.367103143079408	22.206439791751936
72-73	21.784807415687297	27.63370940913079	28.438878209431333	22.142604965750575
74-75	21.88190898681396	27.708288537979087	28.17932133280796	22.230481142398986
76-77	21.933387127693248	27.644465462271988	28.108056019750048	22.31409139028471
78-79	21.927591602730136	27.811163964317398	28.178253887202626	22.08299054574984
80-81	21.905986983045175	27.624455469278058	28.27415439347088	22.195403154205888
82-83	21.79388081306127	27.68001204372641	28.346360699318012	22.179746443894306
84-85	21.748479017627655	27.65104972731884	28.158929755151075	22.44154149990244
86-87	21.90052375950478	27.78581380937733	28.06082784385499	22.2528345872629
88-89	21.65220328636807	27.6888941472607	28.131729072680418	22.52717349369081
90-91	21.584082494265658	27.624563847990274	28.23146007806283	22.559893579681237
92-93	21.75924862201302	27.705245729723536	28.093804965588475	22.441700682674966
94-95	21.52068789439337	27.538735040015034	28.20481209206081	22.735764973530785
96-97	21.527729910333846	27.513486616605608	28.234293162745765	22.724490310314778
98-99	21.44745589095899	27.65921803898128	28.099386822645293	22.79393924741443
100	21.582877893090053	27.494039011084542	28.034668898915417	22.88841419690999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	33.5
2	136.0
3	258.5
4	180.5
5	112.0
6	301.5
7	702.5
8	1204.0
9	1461.5
10	1202.5
11	966.0
12	1081.0
13	1250.5
14	1458.0
15	1687.0
16	1932.5
17	2259.0
18	2598.0
19	3030.5
20	3649.5
21	4431.0
22	5653.0
23	7638.5
24	10261.5
25	13452.5
26	17722.5
27	23372.0
28	30454.5
29	38300.0
30	45931.5
31	53914.0
32	62187.0
33	70717.5
34	79385.5
35	86562.5
36	92458.0
37	97798.0
38	102076.5
39	103750.0
40	104917.0
41	106355.0
42	106011.5
43	105152.5
44	103436.0
45	101045.5
46	99280.0
47	96914.0
48	94745.0
49	92795.5
50	90173.0
51	87229.0
52	81875.5
53	77696.0
54	75255.5
55	71082.0
56	65224.5
57	58991.5
58	53283.0
59	48498.5
60	43483.0
61	37098.0
62	31538.0
63	26351.0
64	20994.0
65	16627.0
66	12999.0
67	9653.0
68	7152.5
69	5413.5
70	3937.5
71	2833.0
72	2099.5
73	1473.5
74	1053.5
75	719.5
76	431.0
77	272.0
78	179.0
79	96.0
80	52.0
81	21.0
82	6.5
83	4.5
84	2.5
85	1.0
86	0.5
87	0.5
88	0.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04560975266825626
2	0.24426937462856346
3	0.00312066728782806
4	0.0021604619684963493
5	1.02879141356969E-4
6	1.02879141356969E-4
7	0.0
8	1.7146523559494837E-4
9	0.0
10-11	5.829818010228245E-4
12-13	0.0
14-15	3.4293047118989675E-4
16-17	1.2002566491646385E-4
18-19	4.286630889873709E-4
20-21	1.7146523559494835E-5
22-23	1.5431871203545352E-4
24-25	0.0014917475496760509
26-27	1.5431871203545352E-4
28-29	3.257839476304019E-4
30-31	8.573261779747419E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.14395706784845E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.05758282713938E-4
70-71	5.14395706784845E-5
72-73	2.914909005114122E-4
74-75	1.3717218847595868E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	6.858609423797934E-5
84-85	0.0
86-87	3.4293047118989675E-4
88-89	0.0019889967329014013
90-91	0.002006143256460896
92-93	4.801026596658554E-4
94-95	4.801026596658554E-4
96-97	1.3717218847595868E-4
98-99	8.573261779747419E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2916043.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.63871008603111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96613055151543	51.042688193350905
2	12.893584910105485	16.668493939478683
3	3.7127533890616355	7.199627698094536
4	1.5855504212952014	4.099517360355401
5	0.7888370776801313	2.549470558463901
6	0.4672899111942736	1.8123010257488323
7	0.3181345889045627	1.4394666622378505
8	0.2145395893314618	1.1094049853418027
9	0.16380386940702638	0.952926374301379
>10	0.8351029600498612	9.548386837824111
>50	0.043047629733791365	1.8714589507484936
>100	0.010694677196863385	1.1093731878266453
>500	3.182547146424233E-4	0.12905234235182772
>1k	1.5912735727144613E-4	0.2745276580573214
>5k	5.304245242381538E-5	0.1933042258183062
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5638	0.19334419965686378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4372	0.14992920200422286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	6.858609423797934E-5	0.0
14-15	0.0	0.0	0.0	1.0287914135696902E-4	0.0
16-17	0.0	0.0	0.0	1.0287914135696902E-4	0.0
18-19	0.0	0.0	0.0	1.0287914135696902E-4	0.0
20-21	0.0	0.0	0.0	1.2002566491646385E-4	0.0
22-23	0.0	0.0	0.0	1.3717218847595868E-4	0.0
24-25	0.0	0.0	0.0	2.400513298329277E-4	0.0
26-27	0.0	0.0	0.0	3.4293047118989675E-4	0.0
28-29	0.0	0.0	0.0	6.858609423797935E-4	0.0
30-31	0.0	0.0	0.0	0.0026577111517216995	0.0
32-33	0.0	0.0	0.0	0.008041719549403078	0.0
34-35	0.0	0.0	0.0	0.015346138585747878	0.0
36-37	0.0	0.0	0.0	0.030675130647936262	0.0
38-39	0.0	0.0	0.0	0.055829080709715186	0.0
40-41	0.0	0.0	0.0	0.08610984131578306	0.0
42-43	0.0	0.0	0.0	0.11843103822543083	0.0
44-45	0.0	0.0	0.0	0.15574187349089158	0.0
46-47	0.0	0.0	0.0	0.20179743577169473	0.0
48-49	0.0	0.0	0.0	0.25241397331932347	0.0
50-51	0.0	0.0	0.0	0.3101806111912616	0.0
52-53	0.0	0.0	0.0	0.3699876853667796	0.0
54-55	0.0	0.0	0.0	0.43408139043217125	0.0
56-57	0.0	0.0	0.0	0.5121152191514322	0.0
58-59	0.0	0.0	0.0	0.6019287095560661	0.0
60-61	0.0	0.0	0.0	0.7012756670597793	0.0
62-63	0.0	1.7146523559494835E-5	0.0	0.801857174259776	0.0
64-65	0.0	3.429304711898967E-5	0.0	0.9078741294281325	0.0
66-67	0.0	3.429304711898967E-5	0.0	1.0269567355488243	0.0
68-69	0.0	3.429304711898967E-5	0.0	1.1672324447890514	0.0
70-71	0.0	3.429304711898967E-5	0.0	1.322442776049599	0.0
72-73	0.0	3.429304711898967E-5	0.0	1.4869636696029516	0.0
74-75	0.0	3.429304711898967E-5	0.0	1.6577087512084012	0.0
76-77	0.0	3.429304711898967E-5	0.0	1.8357925448973145	0.0
78-79	0.0	3.429304711898967E-5	0.0	2.043711289579749	0.0
80-81	0.0	3.429304711898967E-5	0.0	2.277949947926008	0.0
82-83	0.0	3.429304711898967E-5	0.0	2.5155664714134875	0.0
84-85	0.0	3.429304711898967E-5	0.0	2.759630087759337	0.0
86-87	0.0	3.429304711898967E-5	0.0	3.021063132470955	0.0
88	0.0	3.429304711898967E-5	0.0	3.2324626214359666	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4155	0.0	54.45761	1
GTATCAA	7995	0.0	45.34154	1
TCAACGC	9575	0.0	37.058167	4
ATCAACG	9625	0.0	36.965847	3
CAACGCA	9840	0.0	36.012398	5
AACGCAG	10275	0.0	34.71648	6
TATCAAC	10495	0.0	34.12011	2
ACGCAGA	11955	0.0	29.523382	7
CGCAGAG	12320	0.0	28.534262	8
CTATCGG	145	8.661973E-7	25.929714	9
GCAGAGT	13735	0.0	25.389317	9
GTACATG	8580	0.0	25.218184	1
TATACCG	150	1.1640732E-6	25.06539	5
TACATGG	8830	0.0	23.58527	2
AGAGTAC	12455	0.0	21.659275	10-11
ACATGGG	9075	0.0	21.648869	3
GAGTACT	8840	0.0	21.55792	12-13
CAGAGTA	13215	0.0	20.751497	10-11
CGTATAC	160	5.4157823E-5	20.562864	3
GTGGTAT	1975	0.0	20.049362	1
>>END_MODULE