FastQCFastQC Report
Wed 25 May 2016
SRR1033055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4628925
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52550.034.7122271
TCAACGC50300.034.5806964
ATCAACG51350.033.9651453
CAACGCA53250.033.1078075
GGTATCA34650.032.9705731
AACGCAG57150.030.5937546
TATCAAC62450.028.3044932
GTACATG115600.026.8823431
TACATGG113250.026.0273112
ACGCAGA67000.025.6052387
CGCAGAG67950.024.7804818
ACATGGG116200.024.1123833
CATGGGG79450.019.8221574
GCAGAGT87700.018.3992469
GAGTACA84450.016.534091
CCTATAC18150.015.7998583
AGTACAT83400.015.2194072
AGAGTAC73050.015.10930310-11
GTATTAG22600.014.7697581
GTATATA38200.014.0302351
Produced by FastQC (version 0.11.5)