##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033055_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4628925
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84259887554886	33.0	31.0	34.0	30.0	34.0
2	31.981210540244223	34.0	31.0	34.0	30.0	34.0
3	32.04670371630562	34.0	31.0	34.0	30.0	34.0
4	35.443015602974775	37.0	35.0	37.0	35.0	37.0
5	35.5022224382551	37.0	35.0	37.0	35.0	37.0
6	35.512797031708224	37.0	36.0	37.0	35.0	37.0
7	35.4749472069649	37.0	36.0	37.0	35.0	37.0
8	35.46927072700465	37.0	36.0	37.0	35.0	37.0
9	37.145439167841346	39.0	38.0	39.0	35.0	39.0
10-11	37.1453851596213	39.0	38.0	39.0	35.0	39.0
12-13	37.09203313944383	39.0	38.0	39.0	35.0	39.0
14-15	38.51889596396572	41.0	39.0	41.0	35.0	41.0
16-17	38.51724903730348	41.0	39.0	41.0	35.0	41.0
18-19	38.523316320744016	41.0	39.0	41.0	35.0	41.0
20-21	38.546982614753965	41.0	39.0	41.0	34.5	41.0
22-23	38.50072608651037	41.0	39.0	41.0	34.0	41.0
24-25	38.501030584855016	41.0	39.0	41.0	34.0	41.0
26-27	38.40782827546353	40.0	39.0	41.0	34.0	41.0
28-29	38.4131296143273	40.0	39.0	41.0	34.0	41.0
30-31	38.55352095788979	41.0	39.0	41.0	34.0	41.0
32-33	38.53985352970722	41.0	39.0	41.0	34.0	41.0
34-35	38.50392272503875	41.0	39.0	41.0	34.0	41.0
36-37	38.437310714690774	41.0	39.0	41.0	34.0	41.0
38-39	38.358940250706155	41.0	39.0	41.0	34.0	41.0
40-41	38.297341823425526	41.0	39.0	41.0	34.0	41.0
42-43	38.183735100482295	40.5	38.0	41.0	33.0	41.0
44-45	38.055441598211246	40.0	38.0	41.0	33.0	41.0
46-47	37.94214952715804	40.0	38.0	41.0	33.0	41.0
48-49	37.838225721954885	40.0	38.0	41.0	33.0	41.0
50-51	37.726856883617685	40.0	38.0	41.0	33.0	41.0
52-53	37.58503410619096	40.0	37.5	41.0	32.5	41.0
54-55	37.43510231857289	40.0	37.0	41.0	32.0	41.0
56-57	37.27380968583418	40.0	37.0	41.0	32.0	41.0
58-59	37.08828086434755	40.0	36.5	41.0	31.0	41.0
60-61	36.872618480532736	39.5	36.0	41.0	31.0	41.0
62-63	36.628450990240715	39.0	35.5	41.0	31.0	41.0
64-65	36.3581436294604	39.0	35.0	41.0	31.0	41.0
66-67	36.064196330681526	38.5	35.0	40.5	30.0	41.0
68-69	35.75006259552704	38.0	35.0	40.0	30.0	41.0
70-71	35.40917761683328	37.0	35.0	40.0	30.0	41.0
72-73	35.05050492284926	37.0	34.5	39.0	29.5	41.0
74-75	34.681365759868655	36.0	34.0	39.0	29.0	41.0
76-77	34.30309855960077	36.0	34.0	39.0	29.0	40.0
78-79	33.90220774801925	35.0	34.0	37.5	29.0	39.0
80-81	33.53633673045037	35.0	34.0	37.0	29.0	39.0
82-83	33.2976962253655	35.0	34.0	36.5	29.0	39.0
84-85	33.059487678024595	35.0	34.0	36.0	29.0	37.0
86-87	32.871826395977465	35.0	34.0	36.0	29.0	37.0
88-89	32.64573945354483	35.0	34.0	35.5	28.5	37.0
90-91	32.43562479409366	35.0	34.0	35.0	28.0	36.0
92-93	32.21989695231615	35.0	34.0	35.0	27.0	36.0
94-95	32.024828874954764	35.0	33.0	35.0	27.0	36.0
96-97	31.85963058377485	35.0	33.0	35.0	27.0	36.0
98-99	31.693568269090555	35.0	33.0	35.0	26.5	35.0
100	31.561186020512324	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12534742415829925
1101	2	0.20795755021786988
1101	3	0.22144128221234638
1101	4	0.10738190403072423
1101	5	0.1290920756503624
1101	6	0.19282204417572757
1101	7	0.23922516560609353
1101	8	0.2823831951091904
1101	9	0.29466329473500963
1101	10-11	0.31923555153215233
1101	12-13	0.320502953399064
1101	14-15	0.4288229104826584
1101	16-17	0.4236101455404224
1101	18-19	0.39568179248397684
1101	20-21	0.3588702569896256
1101	22-23	0.365449986601071
1101	24-25	0.36787034622276593
1101	26-27	0.2796457512061963
1101	28-29	0.31248321770178933
1101	30-31	0.31487901974997357
1101	32-33	0.31046177286021504
1101	34-35	0.3299294606410115
1101	36-37	0.3577181867351058
1101	38-39	0.42269966181348906
1101	40-41	0.3921965670033032
1101	42-43	0.4072426579101176
1101	44-45	0.3557567500561092
1101	46-47	0.3357870523580644
1101	48-49	0.3816088060043086
1101	50-51	0.3696105486562331
1101	52-53	0.309447328230803
1101	54-55	0.29728014451292495
1101	56-57	0.43425099437070713
1101	58-59	0.4069346872797439
1101	60-61	0.4412677202975033
1101	62-63	0.39139093798552693
1101	64-65	0.372147246155869
1101	66-67	0.37783929130850424
1101	68-69	0.4195746063062771
1101	70-71	0.39724752662427676
1101	72-73	0.4012414103116697
1101	74-75	0.3879321022708382
1101	76-77	0.36309036918789417
1101	78-79	0.35139918656920344
1101	80-81	0.3959228974914666
1101	82-83	0.3529908844617893
1101	84-85	0.38751098977179055
1101	86-87	0.36154260573946573
1101	88-89	0.3856957398622356
1101	90-91	0.4256957159933563
1101	92-93	0.41125471958189763
1101	94-95	0.42856758277767426
1101	96-97	0.3751406962134354
1101	98-99	0.3494071149472937
1101	100	0.31996280197188653
1102	1	-0.0034043625422945922
1102	2	0.086991451717644
1102	3	0.08901878050356515
1102	4	-0.056406088361939055
1102	5	-0.053210556550261856
1102	6	0.09132964213149819
1102	7	0.11971039697278485
1102	8	0.14759323984389994
1102	9	0.17711478677839665
1102	10-11	0.13914196827041536
1102	12-13	0.14550722221094503
1102	14-15	0.20987967163879517
1102	16-17	0.21377275689976472
1102	18-19	0.1260871121686904
1102	20-21	0.2552250000082026
1102	22-23	0.21970226717626673
1102	24-25	0.19783756709428246
1102	26-27	0.06240146111100131
1102	28-29	0.12383704237327464
1102	30-31	0.17902582526919986
1102	32-33	0.14800020242394396
1102	34-35	0.16312773073394737
1102	36-37	0.23607484860812633
1102	38-39	0.2938034329709751
1102	40-41	0.17380102777473638
1102	42-43	0.2148830331335887
1102	44-45	0.199774880046256
1102	46-47	0.16337713196481474
1102	48-49	0.10243795842539072
1102	50-51	0.15569399958096852
1102	52-53	0.1785124588257503
1102	54-55	0.1716681017069135
1102	56-57	0.2117130202086699
1102	58-59	0.1793907486921924
1102	60-61	0.16662669687106302
1102	62-63	0.192576109979278
1102	64-65	0.18929588204316872
1102	66-67	0.1926944946123399
1102	68-69	0.19734536828926252
1102	70-71	0.10245607002890722
1102	72-73	0.16107849058674617
1102	74-75	0.18187644024607152
1102	76-77	0.13388726498148174
1102	78-79	0.08385712252673017
1102	80-81	0.24716575502342408
1102	82-83	0.18419128971503085
1102	84-85	0.22734384451204193
1102	86-87	0.19804069190094253
1102	88-89	0.18852382541782475
1102	90-91	0.18965596166199106
1102	92-93	0.19477356149948832
1102	94-95	0.24175916041117773
1102	96-97	0.24317282859475142
1102	98-99	0.26332849199520325
1102	100	0.1919851584497465
1103	1	0.02403902497837862
1103	2	0.10174815627579648
1103	3	0.09276717794146805
1103	4	0.04892260339929777
1103	5	0.04219982735388328
1103	6	0.10530886094182534
1103	7	0.10364527385194577
1103	8	0.0575061741770071
1103	9	0.17460360423456933
1103	10-11	0.20869589339950068
1103	12-13	0.18756521386492864
1103	14-15	0.20711504342182252
1103	16-17	0.19267429657423918
1103	18-19	0.1764542701517513
1103	20-21	0.17084963738805214
1103	22-23	0.1938806429546034
1103	24-25	0.1488916435439549
1103	26-27	0.05158551131531652
1103	28-29	0.08101445529079143
1103	30-31	0.15933831781219254
1103	32-33	0.09648876005322649
1103	34-35	0.07775408449561638
1103	36-37	0.13804319013733846
1103	38-39	0.19882309722981972
1103	40-41	0.16706107879021914
1103	42-43	0.1409251969077019
1103	44-45	0.16183838896150604
1103	46-47	0.11679567405997915
1103	48-49	0.09798696185664824
1103	50-51	0.06281676803074987
1103	52-53	0.11285456876998978
1103	54-55	0.12127371803950382
1103	56-57	0.13420260942155693
1103	58-59	0.09552497568953555
1103	60-61	0.059117776399247646
1103	62-63	0.07944262399625046
1103	64-65	-0.018371254310245888
1103	66-67	0.011542719516903333
1103	68-69	0.0037787450703063996
1103	70-71	-0.04377052301187234
1103	72-73	-0.011548716851663698
1103	74-75	-0.0103840406769109
1103	76-77	-0.015854229127761243
1103	78-79	-0.007911886838670057
1103	80-81	0.058148736758376174
1103	82-83	0.04739259693663911
1103	84-85	0.054003833106669674
1103	86-87	0.04959793582688121
1103	88-89	0.04389185950780217
1103	90-91	0.06658175043124004
1103	92-93	0.06124760314580868
1103	94-95	0.06512250099267192
1103	96-97	0.05514810915677515
1103	98-99	0.06120517762504818
1103	100	0.038403295497321466
1104	1	-0.11087750940268037
1104	2	-0.03111519426263598
1104	3	0.006422903139238656
1104	4	0.012965523039376592
1104	5	-0.04605149380599016
1104	6	0.03934194897966847
1104	7	0.05678737692215918
1104	8	0.07298988091422132
1104	9	0.1251177094815148
1104	10-11	0.08647749008211036
1104	12-13	0.09888381835126125
1104	14-15	0.11209504791948888
1104	16-17	0.11535222736161899
1104	18-19	0.15676245863305382
1104	20-21	0.13460014717710322
1104	22-23	0.12539089133927206
1104	24-25	0.04226033941833407
1104	26-27	-0.03502641653460614
1104	28-29	-0.05698602390479124
1104	30-31	1.5835044359135964E-4
1104	32-33	0.032668700690848596
1104	34-35	0.05727770670409882
1104	36-37	0.11938356197250499
1104	38-39	0.09278441138323501
1104	40-41	0.16185270841569377
1104	42-43	0.20363680381430527
1104	44-45	0.16738202457471374
1104	46-47	0.163629476329767
1104	48-49	0.11384828648172629
1104	50-51	0.10103498202419559
1104	52-53	0.048774103150805104
1104	54-55	0.07395327720900013
1104	56-57	0.04777762670472896
1104	58-59	0.05777557143460399
1104	60-61	0.041830158258775896
1104	62-63	0.06054780006875404
1104	64-65	0.051662885309291084
1104	66-67	0.011194358554611483
1104	68-69	0.03908312377509304
1104	70-71	-0.02003630194870709
1104	72-73	0.0347234039468205
1104	74-75	0.045057784268585976
1104	76-77	-0.02941394354289173
1104	78-79	0.025640350261610934
1104	80-81	-0.01396567327068965
1104	82-83	0.009653149355457913
1104	84-85	-0.022883281510587494
1104	86-87	-0.02814389547027929
1104	88-89	0.004368855299766494
1104	90-91	0.04500650900030223
1104	92-93	0.02244705861875218
1104	94-95	0.13447931703478133
1104	96-97	0.12711752205881055
1104	98-99	0.09491377864616979
1104	100	0.11911165152541159
1105	1	-0.04972278190047774
1105	2	0.01943148291469754
1105	3	0.06855987042686706
1105	4	0.0899753054880108
1105	5	-0.01592165420209568
1105	6	0.06078929056824478
1105	7	0.025495804767132313
1105	8	0.12341225098983699
1105	9	0.14917875188668006
1105	10-11	0.1524559695312604
1105	12-13	0.18554565704653925
1105	14-15	0.21459619413688102
1105	16-17	0.15926610335089464
1105	18-19	0.18710300713581773
1105	20-21	0.18453337159462535
1105	22-23	0.1542138340822916
1105	24-25	0.13013157101091366
1105	26-27	0.19479476890734304
1105	28-29	0.18107670844521095
1105	30-31	0.1702797031612775
1105	32-33	0.13909866669220605
1105	34-35	0.1541538172110677
1105	36-37	0.20040022776431954
1105	38-39	0.17982379341528087
1105	40-41	0.19808839115015076
1105	42-43	0.19003934421294844
1105	44-45	0.15261154444549874
1105	46-47	0.18260031479417194
1105	48-49	0.1999561317184586
1105	50-51	0.12476976473993062
1105	52-53	0.16433241902183227
1105	54-55	0.1466189305900727
1105	56-57	0.13425813775742057
1105	58-59	0.12228926170645593
1105	60-61	0.1457225592207152
1105	62-63	0.1334072536098958
1105	64-65	0.12980048992630344
1105	66-67	0.05994506289184898
1105	68-69	0.07020460981167531
1105	70-71	0.026731690180369583
1105	72-73	0.04333283205518512
1105	74-75	0.07337669330189556
1105	76-77	0.05533634829543388
1105	78-79	0.09766024311409183
1105	80-81	0.1442311165773802
1105	82-83	0.16542924885316523
1105	84-85	0.15042151290655426
1105	86-87	0.04055863289714523
1105	88-89	0.14009149252417075
1105	90-91	0.1381733578384967
1105	92-93	0.16574678505951823
1105	94-95	0.14227382754769735
1105	96-97	0.1636941899502773
1105	98-99	0.1032185475143983
1105	100	0.13641677018155818
1106	1	-0.012532786492858605
1106	2	0.05547952176242177
1106	3	0.03042507556344276
1106	4	0.01531830540825041
1106	5	0.010700605594607282
1106	6	0.08260650844300699
1106	7	0.0553654593650279
1106	8	0.08136540692123617
1106	9	0.08221052553831498
1106	10-11	0.11748191574181988
1106	12-13	0.10730272963807153
1106	14-15	0.12205443008782879
1106	16-17	0.148376574133259
1106	18-19	0.17202051075588543
1106	20-21	0.12794906189049726
1106	22-23	0.14804836839270052
1106	24-25	0.09829922274172986
1106	26-27	0.06751954545158156
1106	28-29	0.08702985483674297
1106	30-31	0.13328147660356393
1106	32-33	0.09692764986415625
1106	34-35	0.12919551214031344
1106	36-37	0.10921420588500297
1106	38-39	0.14845072756666866
1106	40-41	0.14457624757101684
1106	42-43	0.1079273190517398
1106	44-45	0.10690680452195522
1106	46-47	0.0927469583402214
1106	48-49	0.15626825508721964
1106	50-51	0.09384169360507855
1106	52-53	0.11651983380902209
1106	54-55	0.11567842672117479
1106	56-57	0.14615061099348026
1106	58-59	0.14010230006261537
1106	60-61	0.17761272080738877
1106	62-63	0.13657804245296035
1106	64-65	0.1413959687747166
1106	66-67	0.12982826850736018
1106	68-69	0.1639107225917229
1106	70-71	0.15043015075615074
1106	72-73	0.15581943981439395
1106	74-75	0.1467314583426571
1106	76-77	0.08449532749863664
1106	78-79	0.06299555019732139
1106	80-81	0.09138360531924405
1106	82-83	0.0710082869897306
1106	84-85	0.09147984199480419
1106	86-87	0.05791958789365026
1106	88-89	0.09515837710007702
1106	90-91	0.12641007020491912
1106	92-93	0.1986142964572224
1106	94-95	0.15342459876145398
1106	96-97	0.1352016759578163
1106	98-99	0.1287138009482831
1106	100	0.13193590683130552
1107	1	-0.13196767571085033
1107	2	-0.059892498712923015
1107	3	-0.09572121881861406
1107	4	-0.08718612843635043
1107	5	-0.09292793764520013
1107	6	-0.03532009592773733
1107	7	-0.06104537758593409
1107	8	-0.052595005225590796
1107	9	-0.11796169974092408
1107	10-11	-0.074880122225089
1107	12-13	-0.05622635003492604
1107	14-15	0.009652486468588961
1107	16-17	-0.02277562853852544
1107	18-19	-0.0017840747158999193
1107	20-21	0.0062438634783106295
1107	22-23	0.019633551385744852
1107	24-25	-0.014058287848421003
1107	26-27	-0.036086318867511125
1107	28-29	-0.019201152355357465
1107	30-31	0.034919256196403126
1107	32-33	-0.015439192923203393
1107	34-35	0.012813869833337321
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2216	38-39	-0.7898000453656309
2216	40-41	-0.6898227091877587
2216	42-43	-0.7888149308727108
2216	44-45	-0.6154545212697897
2216	46-47	-0.6221393091391363
2216	48-49	-0.6742938634707372
2216	50-51	-0.5835006201685147
2216	52-53	-0.5930808826400664
2216	54-55	-0.6131812065432456
2216	56-57	-0.6680207330769647
2216	58-59	-0.6287706181550377
2216	60-61	-0.6132789164310282
2216	62-63	-0.6310838254659288
2216	64-65	-0.6170674552784305
2216	66-67	-0.6163145836201878
2216	68-69	-0.653707835526582
2216	70-71	-0.6286903256254135
2216	72-73	-0.650619021388394
2216	74-75	-0.7234433295131453
2216	76-77	-0.5517686903581236
2216	78-79	-0.6170317251339057
2216	80-81	-0.5951518206211617
2216	82-83	-0.7455217514739729
2216	84-85	-0.6172827970025949
2216	86-87	-0.6205687130464668
2216	88-89	-0.7369020636952612
2216	90-91	-0.679545519531839
2216	92-93	-0.8788664446259631
2216	94-95	-0.7806869220064172
2216	96-97	-0.695197194181354
2216	98-99	-0.6447644583520891
2216	100	-0.6286694985599119
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39817.0
3	8112.0
4	4201.0
5	3570.0
6	3305.0
7	3745.0
8	4514.0
9	5065.0
10	5547.0
11	6239.0
12	7163.0
13	7786.0
14	8658.0
15	9122.0
16	8747.0
17	8920.0
18	8899.0
19	8662.0
20	8713.0
21	9308.0
22	10615.0
23	14388.0
24	21647.0
25	28614.0
26	29637.0
27	27746.0
28	30910.0
29	38951.0
30	47757.0
31	58883.0
32	77580.0
33	105011.0
34	151311.0
35	231417.0
36	417677.0
37	911469.0
38	1582884.0
39	671495.0
40	840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.228316625962854	20.175646797238798	13.631714129952172	23.96432244684618
2	15.16982598447677	20.629193726130598	40.72274892527579	23.478231364116844
3	17.598755811867342	26.105276127194244	30.466224701760154	25.829743359178263
4	11.958341287443863	17.26910721478001	40.16557377368154	30.606977724094588
5	12.493807919333324	38.284237422157084	36.60239155399116	12.61956310451843
6	29.96468691093499	39.23934585594456	18.316059498619865	12.479907734500577
7	26.40749542736291	32.80823595780395	23.631630648117657	17.152637966715485
8	24.54719202397099	36.60924049187588	21.208687408309572	17.63488007584355
9	24.761307937142245	16.99514733895084	21.552617995607285	36.69092672829963
10-11	23.224487386257035	27.197960227860047	29.868765518759616	19.708786867123305
12-13	24.100293358506153	24.671396302147368	29.491342517952397	21.736967821394085
14-15	21.48418289956458	26.63542923232165	26.48252201643323	25.39786585168054
16-17	21.14603837019993	29.888539216335918	27.042019907950348	21.9234025055138
18-19	21.048204859842187	29.03181581297587	28.82068709008462	21.099292237097327
20-21	22.399876576898777	28.3348346783453	28.127320399710946	21.13796834504498
22-23	21.909462862585023	28.09783776989509	28.185931708230193	21.806767659289694
24-25	21.815752882237465	28.299363752359323	28.137422575825184	21.747460789578028
26-27	21.58398602775836	28.176520747350448	28.24051690368546	21.99897632120573
28-29	21.62097893859057	28.15117780356268	28.380799102440562	21.847044155406188
30-31	21.881977750757986	28.699905046378532	27.89462612660137	21.523491076262115
32-33	21.54581652807372	28.291207764894704	28.365231618562692	21.797744088468882
34-35	21.6501847844207	28.064824667366977	28.467086095484177	21.81790445272815
36-37	21.956539289078798	28.41990240452571	27.829613297953454	21.79394500844204
38-39	21.789761416959134	28.570911247748736	28.015463129082775	21.623864206209355
40-41	21.62605735693845	28.266542160662038	28.301286514001955	21.806113968397554
42-43	21.56051892481808	28.07636255382406	28.296079958715698	22.06703856264216
44-45	21.711637837570233	28.20648076906031	28.25728506593641	21.824596327433042
46-47	21.74307236603216	28.365639536561805	28.14291877594495	21.74836932146109
48-49	21.671784557600514	28.217139535142653	28.15159983141065	21.959476075846183
50-51	21.701310615380343	28.11744464536509	28.104970726826263	22.076274012428303
52-53	21.76333381400448	28.03177920893348	28.1720025848145	22.032884392247546
54-55	21.831771757256238	28.499213771557507	27.908526417232434	21.760488053953818
56-57	21.688745104164358	28.184048290625775	28.01121117312518	22.115995432084688
58-59	21.557388088239712	27.832557520170674	28.371987801722433	22.23806658986718
60-61	21.80322946596951	28.260875204387006	28.01347073558569	21.922424594057794
62-63	21.70095752091959	28.23827932674665	28.000198886774513	22.060564265559243
64-65	21.609251209979977	27.961773085168453	28.146655906965623	22.282319797885947
66-67	21.485832886657363	28.057174091648072	28.026790397801566	22.430202623893
68-69	21.97004031367644	28.11688966534687	27.93655522083025	21.976514800146436
70-71	21.82995428432345	27.959386996204017	28.036761465071898	22.17389725440064
72-73	21.62315137700696	28.071433705178983	28.142636121392123	22.16277879642194
74-75	21.692474352723693	28.07225020851466	27.989460403938587	22.245815034823053
76-77	21.665571685527755	28.094301598549976	27.92226761497545	22.317859100946812
78-79	21.834107809001527	28.111553891640266	27.966694988577935	22.087643310780273
80-81	21.79611815206869	27.872476910152272	28.10338079072503	22.22802414705401
82-83	21.596013629551692	27.9811186032314	28.238501808415293	22.18436595880161
84-85	21.569474361134215	27.887108397840098	28.070608634874294	22.472808606151393
86-87	21.77640930415104	28.021302111732886	27.924826017769846	22.277462566346227
88-89	21.59041421645277	27.836641504809727	28.08708580137744	22.485858477360058
90-91	21.487953913708363	27.780850226035657	28.198029627766232	22.533166232489744
92-93	21.71790930925927	27.866486119841465	28.02813323249164	22.387471338407618
94-95	21.502058580364597	27.63122619119787	28.195003065097087	22.671712163340445
96-97	21.46492055032317	27.65108535766727	28.22030432980315	22.66368976220641
98-99	21.46320167151418	27.74563332049188	28.08817053594247	22.70299447205147
100	21.56329472060201	27.58792260277792	28.08173818242495	22.767044494195122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	329.5
2	310.5
3	345.5
4	374.5
5	407.0
6	406.0
7	373.5
8	349.5
9	298.5
10	263.5
11	257.0
12	252.0
13	271.5
14	306.5
15	337.5
16	404.5
17	541.0
18	739.5
19	1030.0
20	1502.5
21	2211.5
22	3331.0
23	5286.5
24	7948.5
25	11565.5
26	17189.0
27	25302.5
28	36232.0
29	49541.0
30	64227.0
31	81103.5
32	99645.5
33	118753.5
34	136296.5
35	150779.5
36	163211.0
37	174485.0
38	181936.0
39	184590.0
40	186999.5
41	188125.0
42	187484.5
43	186492.5
44	181883.5
45	176314.5
46	170777.5
47	163084.0
48	156435.0
49	151662.5
50	145793.0
51	137843.5
52	126689.5
53	117131.0
54	111582.5
55	105567.0
56	96519.5
57	86595.5
58	77352.5
59	67264.5
60	57425.0
61	47527.0
62	39305.0
63	32359.0
64	25763.5
65	20330.0
66	15827.5
67	11935.0
68	9100.5
69	7157.5
70	5424.5
71	3776.5
72	2597.0
73	1921.0
74	1357.5
75	856.0
76	541.0
77	375.0
78	253.0
79	156.0
80	109.5
81	85.5
82	56.5
83	31.0
84	22.5
85	20.5
86	17.5
87	14.5
88	11.0
89	7.5
90	5.0
91	3.0
92	2.5
93	3.5
94	2.5
95	3.5
96	3.0
97	1.5
98	1.5
99	1.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06098608208169284
2	0.04156472615132023
3	0.056492598173441995
4	0.07418569106217966
5	0.08783896909109566
6	0.08665078824997165
7	0.0892647861004445
8	0.100865751767419
9	0.09617783826698424
10-11	0.10116819779970511
12-13	0.1056400784199355
14-15	0.10540244225171069
16-17	0.0753198636832526
18-19	0.09710677965186301
20-21	0.09036655378948677
22-23	0.05646019324141134
24-25	0.02181932090064108
26-27	0.18006340565033999
28-29	0.06583602024228087
30-31	0.07580593766371242
32-33	0.07321354310126002
34-35	0.020836371295711205
36-37	0.05138342055660872
38-39	0.0749742110749256
40-41	0.0802130084198815
42-43	0.0742721042142614
44-45	0.07198215568409512
46-47	0.07834432400611373
48-49	0.07527665710721172
50-51	0.07071836333489957
52-53	0.07448813709446578
54-55	0.0657388054461889
56-57	0.06875246412503982
58-59	0.06537154954984149
60-61	0.06258472539520514
62-63	0.06864444768493765
64-65	0.06528513639775974
66-67	0.06672175505111878
68-69	0.0664409123068531
70-71	0.058804149991628724
72-73	0.05784280367471929
74-75	0.05944144698823162
76-77	0.05429986443936767
78-79	0.05085414000010802
80-81	0.045539731147080584
82-83	0.05099456137224086
84-85	0.04224522972396399
86-87	0.04044135517425752
88-89	0.04208320506381071
90-91	0.038432249388356904
92-93	0.04040895024222687
94-95	0.03950161214536853
96-97	0.04258008068828076
98-99	0.04282851850051578
100	0.049665959158983995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4628925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.719789667293384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84354349060698	41.4679421923088
2	15.29378175392258	17.655077321078014
3	5.704995739720129	9.878734624483522
4	2.670182502806042	6.164894897410073
5	1.4408709195112093	4.158338320595331
6	0.8403112387133619	2.910155277215881
7	0.5291936786814083	2.1381463478726492
8	0.3583090489616894	1.6545218353565312
9	0.25364973981985445	1.317654866840912
>10	1.0079830322023107	9.504507162267052
>50	0.04080055496442664	1.6121823181544066
>100	0.01626600112154962	1.5035125176540853
>500	1.1229896843618869E-4	0.03433231876290705
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.320657604087342E-5	0.0
5	0.0	0.0	0.0	4.320657604087342E-5	0.0
6	0.0	0.0	0.0	4.320657604087342E-5	0.0
7	0.0	0.0	0.0	4.320657604087342E-5	0.0
8	0.0	0.0	0.0	6.480986406131013E-5	0.0
9	0.0	0.0	0.0	1.5122301614305697E-4	0.0
10-11	0.0	0.0	0.0	2.3763616822480382E-4	0.0
12-13	0.0	0.0	0.0	2.700411002554589E-4	0.0
14-15	0.0	0.0	0.0	3.240493203065507E-4	0.0
16-17	0.0	0.0	0.0	3.4565260832698737E-4	0.0
18-19	0.0	0.0	0.0	3.6725589634742405E-4	0.0
20-21	0.0	0.0	0.0	4.2126411639851585E-4	0.0
22-23	0.0	0.0	0.0	4.968756244700444E-4	0.0
24-25	0.0	0.0	0.0	5.832887765517911E-4	0.0
26-27	0.0	0.0	0.0	7.345117926948481E-4	0.0
28-29	0.0	0.0	0.0	0.0012961972812262027	0.0
30-31	0.0	0.0	0.0	0.00439626911215887	0.0
32-33	0.0	0.0	0.0	0.012033031427383248	0.0
34-35	0.0	0.0	0.0	0.023893236550603	0.0
36-37	0.0	0.0	0.0	0.04458918647418137	0.0
38-39	0.0	0.0	0.0	0.07928406703500274	0.0
40-41	0.0	0.0	0.0	0.11943377782098435	0.0
42-43	0.0	0.0	0.0	0.1620030568652549	0.0
44-45	0.0	0.0	0.0	0.2137753366062315	0.0
46-47	0.0	0.0	0.0	0.27754824284256063	0.0
48-49	0.0	0.0	0.0	0.34658154971186617	0.0
50-51	0.0	0.0	0.0	0.4219986281912107	0.0
52-53	0.0	0.0	0.0	0.49889553189995517	0.0
54-55	0.0	0.0	0.0	0.5846713869850991	0.0
56-57	0.0	0.0	0.0	0.6879135004347662	0.0
58-59	0.0	0.0	0.0	0.8027241746193772	0.0
60-61	0.0	0.0	0.0	0.9237565957538738	0.0
62-63	0.0	0.0	0.0	1.0525662005757277	0.0
64-65	0.0	0.0	0.0	1.1920046231036363	0.0
66-67	0.0	0.0	0.0	1.349449386196579	0.0
68-69	0.0	0.0	0.0	1.5303445184357058	0.0
70-71	0.0	0.0	0.0	1.7235103182704408	0.0
72-73	0.0	0.0	0.0	1.9281366624000174	0.0
74-75	0.0	0.0	0.0	2.1465891108626733	0.0
76-77	0.0	0.0	0.0	2.380088249431563	0.0
78-79	0.0	0.0	0.0	2.6458735019469963	0.0
80-81	0.0	0.0	0.0	2.9377987329671575	0.0
82-83	0.0	0.0	0.0	3.240071938949108	0.0
84-85	0.0	0.0	0.0	3.5458016710143285	0.0
86-87	0.0	0.0	0.0	3.874538904821314	0.0
88	0.0	0.0	0.0	4.141350313517718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5255	0.0	34.712227	1
TCAACGC	5030	0.0	34.580696	4
ATCAACG	5135	0.0	33.965145	3
CAACGCA	5325	0.0	33.107807	5
GGTATCA	3465	0.0	32.970573	1
AACGCAG	5715	0.0	30.593754	6
TATCAAC	6245	0.0	28.304493	2
GTACATG	11560	0.0	26.882343	1
TACATGG	11325	0.0	26.027311	2
ACGCAGA	6700	0.0	25.605238	7
CGCAGAG	6795	0.0	24.780481	8
ACATGGG	11620	0.0	24.112383	3
CATGGGG	7945	0.0	19.822157	4
GCAGAGT	8770	0.0	18.399246	9
GAGTACA	8445	0.0	16.53409	1
CCTATAC	1815	0.0	15.799858	3
AGTACAT	8340	0.0	15.219407	2
AGAGTAC	7305	0.0	15.109303	10-11
GTATTAG	2260	0.0	14.769758	1
GTATATA	3820	0.0	14.030235	1
>>END_MODULE