FastQCFastQC Report
Wed 25 May 2016
SRR1033055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4628925
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49350.10661222638085517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.050.305731
GTATCAA98400.044.1265031
TCAACGC113400.037.301014
ATCAACG114600.036.992453
CAACGCA116850.036.1583025
TATCAAC123000.034.592912
AACGCAG122550.034.5532236
ACGCAGA139400.030.1405967
CGCAGAG141500.029.3611418
GTACATG111950.027.288891
TACATGG111100.026.4651872
GCAGAGT162900.025.2443439
ACATGGG115550.024.4453183
AGAGTAC152300.020.44364710-11
GAGTACT111500.020.1897412-13
CAGAGTA164550.019.66429710-11
CATGGGG79900.018.9997064
AGTACTT119650.018.51991812-13
GTACTTT124400.018.02064714-15
GTGGTAT28650.017.9364661
Produced by FastQC (version 0.11.5)