##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033055_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4628925
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70584487759037	34.0	31.0	34.0	31.0	34.0
2	32.70093725865077	34.0	31.0	34.0	31.0	34.0
3	32.81115269744055	34.0	31.0	34.0	31.0	34.0
4	36.30306734284958	37.0	37.0	37.0	35.0	37.0
5	36.28947196163256	37.0	37.0	37.0	35.0	37.0
6	36.351928363496924	37.0	37.0	37.0	35.0	37.0
7	36.299475796216186	37.0	37.0	37.0	35.0	37.0
8	36.30329374530804	37.0	37.0	37.0	35.0	37.0
9	38.110321726966845	39.0	39.0	39.0	37.0	39.0
10-11	38.072878044038305	39.0	39.0	39.0	35.0	39.0
12-13	38.049665527093225	39.0	39.0	39.0	35.0	39.0
14-15	39.53666801687217	41.0	39.0	41.0	37.0	41.0
16-17	39.53280092030007	41.0	39.0	41.0	37.0	41.0
18-19	39.55265250571137	41.0	39.5	41.0	37.0	41.0
20-21	39.54933499678651	41.0	40.0	41.0	37.0	41.0
22-23	39.48081617222141	41.0	39.0	41.0	36.5	41.0
24-25	39.434056503399816	41.0	39.0	41.0	36.0	41.0
26-27	39.3272687503038	41.0	39.0	41.0	36.0	41.0
28-29	39.27889121124235	41.0	39.0	41.0	36.0	41.0
30-31	39.285979898140496	41.0	39.0	41.0	36.0	41.0
32-33	39.26165837640489	41.0	39.0	41.0	35.5	41.0
34-35	39.191860529172544	41.0	39.0	41.0	35.0	41.0
36-37	39.11091117268048	41.0	39.0	41.0	35.0	41.0
38-39	39.025919408934044	41.0	39.0	41.0	35.0	41.0
40-41	38.93496416554599	41.0	39.0	41.0	35.0	41.0
42-43	38.832599037573516	41.0	39.0	41.0	35.0	41.0
44-45	38.69030509243507	41.0	39.0	41.0	35.0	41.0
46-47	38.529537203562384	41.0	38.5	41.0	35.0	41.0
48-49	38.36146988771691	40.5	38.0	41.0	34.5	41.0
50-51	38.243563894424724	40.0	38.0	41.0	34.0	41.0
52-53	38.12335823112278	40.0	38.0	41.0	34.0	41.0
54-55	37.986490707885736	40.0	38.0	41.0	34.0	41.0
56-57	37.84490988728484	40.0	37.5	41.0	33.5	41.0
58-59	37.67572470930075	40.0	37.0	41.0	33.0	41.0
60-61	37.49332847259353	40.0	37.0	41.0	33.0	41.0
62-63	37.26957047262593	39.5	36.0	41.0	33.0	41.0
64-65	37.00919792392402	39.0	36.0	41.0	32.5	41.0
66-67	36.732627229864384	39.0	35.0	41.0	32.0	41.0
68-69	36.426586302435226	38.5	35.0	40.5	32.0	41.0
70-71	36.09427448057595	37.5	35.0	40.0	31.5	41.0
72-73	35.7416091209082	37.0	35.0	39.5	31.0	41.0
74-75	35.38437650210362	36.5	35.0	39.0	31.0	41.0
76-77	35.01635952191924	36.0	35.0	39.0	31.0	40.5
78-79	34.63790005238798	35.5	35.0	37.5	31.0	39.5
80-81	34.29019081104144	35.0	34.0	37.0	30.5	39.0
82-83	33.97681524328003	35.0	34.0	37.0	30.0	39.0
84-85	33.742593906792614	35.0	34.0	36.0	30.0	37.5
86-87	33.5214111267735	35.0	34.0	36.0	30.0	37.0
88-89	33.304394324816236	35.0	34.0	36.0	30.0	37.0
90-91	33.11756314911129	35.0	34.0	35.0	30.0	36.0
92-93	32.9428968929071	35.0	34.0	35.0	30.0	36.0
94-95	32.78768936632155	35.0	34.0	35.0	30.0	36.0
96-97	32.64381924528914	35.0	34.0	35.0	29.0	36.0
98-99	32.51991682734112	35.0	34.0	35.0	29.0	35.5
100	32.415001323201395	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16228295901863987
1101	2	0.07213665628282229
1101	3	0.1565517805026886
1101	4	0.1336468727555129
1101	5	0.13343233755463046
1101	6	0.16586665351636753
1101	7	0.20315519832124096
1101	8	0.2140516988811214
1101	9	0.24193095493970418
1101	10-11	0.27685466675384873
1101	12-13	0.2795865047029551
1101	14-15	0.39205534128427644
1101	16-17	0.3705541963555561
1101	18-19	0.347339084737591
1101	20-21	0.3298753659189302
1101	22-23	0.36123912943644854
1101	24-25	0.3844606259540768
1101	26-27	0.4117834256348516
1101	28-29	0.43159384254211375
1101	30-31	0.3897034855950423
1101	32-33	0.3611094285765759
1101	34-35	0.35033244591073043
1101	36-37	0.3672321466072006
1101	38-39	0.3156761765068765
1101	40-41	0.34682423640178683
1101	42-43	0.38457967876531995
1101	44-45	0.3448509049689079
1101	46-47	0.35358185651188734
1101	48-49	0.3037162077071187
1101	50-51	0.3419236336512341
1101	52-53	0.36294928282885763
1101	54-55	0.3193559513436739
1101	56-57	0.33636986618270015
1101	58-59	0.3119858922364642
1101	60-61	0.37655438696727117
1101	62-63	0.39535412838412753
1101	64-65	0.34482758259807866
1101	66-67	0.38239119469512417
1101	68-69	0.33017632575913325
1101	70-71	0.3232500674041958
1101	72-73	0.32547440984160403
1101	74-75	0.3349897361143874
1101	76-77	0.3081072601248991
1101	78-79	0.3110657435220361
1101	80-81	0.29204501363805946
1101	82-83	0.3454349290521037
1101	84-85	0.37016708368092566
1101	86-87	0.36881836611291874
1101	88-89	0.39326952159433404
1101	90-91	0.38196043596737184
1101	92-93	0.4195492751174825
1101	94-95	0.40164342423176436
1101	96-97	0.4378310593499677
1101	98-99	0.46560607136220256
1101	100	0.47302444429770674
1102	1	0.12630760147801823
1102	2	0.14004172087111755
1102	3	0.12210761024920913
1102	4	0.08675624727286646
1102	5	0.08516409878289721
1102	6	0.10865229261646192
1102	7	0.1393528517094964
1102	8	0.15345068987189592
1102	9	0.19764624357001281
1102	10-11	0.19854313742549579
1102	12-13	0.19570535043739312
1102	14-15	0.3031688578527607
1102	16-17	0.32478275263318324
1102	18-19	0.27185353402062873
1102	20-21	0.25416357015742364
1102	22-23	0.22098377150514636
1102	24-25	0.26897513664553685
1102	26-27	0.28660672090987305
1102	28-29	0.29274346699916975
1102	30-31	0.2447980938745431
1102	32-33	0.2498994213751189
1102	34-35	0.2607106230720788
1102	36-37	0.2640601976999619
1102	38-39	0.22046572388259023
1102	40-41	0.27161028561486944
1102	42-43	0.2717556093735851
1102	44-45	0.28257940154537664
1102	46-47	0.2520401821482139
1102	48-49	0.2748724989485325
1102	50-51	0.22601279836511878
1102	52-53	0.21811091099169033
1102	54-55	0.20755684677907027
1102	56-57	0.20779481398486865
1102	58-59	0.2149901352673993
1102	60-61	0.2346568169065435
1102	62-63	0.21784627650981747
1102	64-65	0.20168327595172997
1102	66-67	0.2668846888828824
1102	68-69	0.2909000139766036
1102	70-71	0.2982417779287019
1102	72-73	0.22819653145606367
1102	74-75	0.2794326747583966
1102	76-77	0.2771828910309395
1102	78-79	0.2858915454253861
1102	80-81	0.24226386452604487
1102	82-83	0.20026704278855334
1102	84-85	0.20723176842953706
1102	86-87	0.2544629241500047
1102	88-89	0.25740997451798364
1102	90-91	0.1866753218166579
1102	92-93	0.2255667871588969
1102	94-95	0.30251279811173504
1102	96-97	0.3080057369352289
1102	98-99	0.3016487558282819
1102	100	0.3506623364503838
1103	1	0.11945077003462501
1103	2	0.10960777926902665
1103	3	0.1294471381141591
1103	4	0.09344893175322255
1103	5	0.14989370681565362
1103	6	0.13195985699088197
1103	7	0.12178598081225545
1103	8	0.1331761357893484
1103	9	0.1733459711520453
1103	10-11	0.22594397921960763
1103	12-13	0.22136507838669672
1103	14-15	0.27799672413483023
1103	16-17	0.25651299998997956
1103	18-19	0.29632813266173486
1103	20-21	0.3257866856611571
1103	22-23	0.3022332828431118
1103	24-25	0.27031883734213835
1103	26-27	0.2082908953497551
1103	28-29	0.25941456172922983
1103	30-31	0.22074275031159374
1103	32-33	0.18446454151634128
1103	34-35	0.26054345813744106
1103	36-37	0.21508362127720915
1103	38-39	0.24574136379550993
1103	40-41	0.20558057122930506
1103	42-43	0.19166973069026483
1103	44-45	0.2179741825364232
1103	46-47	0.2554730353111836
1103	48-49	0.2037107494570023
1103	50-51	0.25396449670176935
1103	52-53	0.22846798843813332
1103	54-55	0.20429204092378228
1103	56-57	0.17732124232369273
1103	58-59	0.18794799729949574
1103	60-61	0.21209215948686477
1103	62-63	0.15266306479009017
1103	64-65	0.1733342795589934
1103	66-67	0.14532400319053096
1103	68-69	0.176006871526738
1103	70-71	0.20026486495095952
1103	72-73	0.20555980078273706
1103	74-75	0.2175580934220278
1103	76-77	0.239392413503694
1103	78-79	0.23996356788503448
1103	80-81	0.23202395087481165
1103	82-83	0.17566760754311161
1103	84-85	0.2131554200401098
1103	86-87	0.2056248802958578
1103	88-89	0.19331031690174427
1103	90-91	0.20461998904679746
1103	92-93	0.2168129431774375
1103	94-95	0.19030177560480865
1103	96-97	0.21152759212635175
1103	98-99	0.18826940746512832
1103	100	0.1799640220719141
1104	1	0.08650597586677833
1104	2	0.06101040729136997
1104	3	0.041346197061344014
1104	4	0.03967198037391029
1104	5	0.049154509622752585
1104	6	0.05239872558902903
1104	7	0.09546867731494046
1104	8	0.1064070298275297
1104	9	0.10711160233281447
1104	10-11	0.12232352664219093
1104	12-13	0.11975069938026905
1104	14-15	0.1540014469334281
1104	16-17	0.18798723513472737
1104	18-19	0.21469542454702406
1104	20-21	0.19831391753173477
1104	22-23	0.20344271215648746
1104	24-25	0.16888946608646904
1104	26-27	0.17168999096006132
1104	28-29	0.17266957985601294
1104	30-31	0.17219458539278776
1104	32-33	0.14332134615487746
1104	34-35	0.1310074146106004
1104	36-37	0.13628234839249131
1104	38-39	0.08655824511514254
1104	40-41	0.10771230488975192
1104	42-43	0.14738363523420617
1104	44-45	0.14477586840238388
1104	46-47	0.10523823192973936
1104	48-49	0.04398093336364184
1104	50-51	0.16176569773168126
1104	52-53	0.2144440625020536
1104	54-55	0.17017135377594883
1104	56-57	0.17187859582999465
1104	58-59	0.16814027391257014
1104	60-61	0.19240825690236107
1104	62-63	0.18777613312057895
1104	64-65	0.1428936746738998
1104	66-67	0.22634805444337047
1104	68-69	0.20532026322840835
1104	70-71	0.19980251724086173
1104	72-73	0.17911975553137438
1104	74-75	0.06821186186364514
1104	76-77	0.10584280045289773
1104	78-79	0.09056544390875132
1104	80-81	0.1273830741185762
1104	82-83	0.15498442031864812
1104	84-85	0.12629131367732782
1104	86-87	0.11326416275811368
1104	88-89	0.14750862441434975
1104	90-91	0.153184756596481
1104	92-93	0.17030842233255328
1104	94-95	0.18876899963265714
1104	96-97	0.19937072644800935
1104	98-99	0.2147014702108052
1104	100	0.2522318409006701
1105	1	0.021558116564207808
1105	2	0.084595124950269
1105	3	0.11642968395916142
1105	4	0.01740795599188516
1105	5	0.04090248765811566
1105	6	0.03394227975947217
1105	7	0.06481220482159955
1105	8	0.04741550602964395
1105	9	0.09035072209360351
1105	10-11	0.06826238668660523
1105	12-13	0.10834968030358993
1105	14-15	0.14937444488533203
1105	16-17	0.1731312358073609
1105	18-19	0.1448715076934235
1105	20-21	0.1641809107909893
1105	22-23	0.14999133198068648
1105	24-25	0.1271611374920738
1105	26-27	0.10096463858241123
1105	28-29	0.12610904494526665
1105	30-31	0.14607453241826107
1105	32-33	0.17023446078637505
1105	34-35	0.1603195262380055
1105	36-37	0.21176809139917196
1105	38-39	0.22100489967007775
1105	40-41	0.17875934137778415
1105	42-43	0.1387883054836081
1105	44-45	0.13261860133768266
1105	46-47	0.1275783224100806
1105	48-49	0.1934361839683305
1105	50-51	0.1738309422371458
1105	52-53	0.24989845930834775
1105	54-55	0.22382719497874604
1105	56-57	0.2517331109974563
1105	58-59	0.19817834644641152
1105	60-61	0.20819463171057606
1105	62-63	0.20990748893665057
1105	64-65	0.15629215465261126
1105	66-67	0.1752324957558713
1105	68-69	0.17960829616142604
1105	70-71	0.22064390816115065
1105	72-73	0.19306658498025797
1105	74-75	0.16629697042100133
1105	76-77	0.1026053576302175
1105	78-79	0.14128443692831638
1105	80-81	0.1609726321362146
1105	82-83	0.17629598101846256
1105	84-85	0.17074102016671588
1105	86-87	0.14832505976670518
1105	88-89	0.1318852052536883
1105	90-91	0.15763363622994575
1105	92-93	0.19050080104370437
1105	94-95	0.1596955381304639
1105	96-97	0.14215489893944522
1105	98-99	0.10949353220827618
1105	100	0.06874977415110095
1106	1	0.03337340968391089
1106	2	0.06903009274132188
1106	3	0.06194034515006308
1106	4	0.055303547702131084
1106	5	0.050853533160506004
1106	6	0.08169453419177586
1106	7	0.08249742672182947
1106	8	0.08074786476618101
1106	9	0.08370395722349855
1106	10-11	0.09595419285680151
1106	12-13	0.08325101562780901
1106	14-15	0.1558390911804537
1106	16-17	0.15549466277589374
1106	18-19	0.1408365002942844
1106	20-21	0.13075026058152872
1106	22-23	0.14329644823403953
1106	24-25	0.137378570331677
1106	26-27	0.1516028772910829
1106	28-29	0.14707244536118935
1106	30-31	0.11799187907229935
1106	32-33	0.18003348685500953
1106	34-35	0.1684229747118522
1106	36-37	0.1298074452644684
1106	38-39	0.08528535441399754
1106	40-41	0.1345109317298494
1106	42-43	0.08985116512197067
1106	44-45	0.0743803625936792
1106	46-47	0.1253539229994587
1106	48-49	0.15304446142208405
1106	50-51	0.1163894805398229
1106	52-53	0.10927906977281765
1106	54-55	0.08269552380110667
1106	56-57	0.0024911544463961377
1106	58-59	0.0035445132097038368
1106	60-61	0.06749799580966709
1106	62-63	0.07464183784695422
1106	64-65	0.06149893950694718
1106	66-67	0.072001716210643
1106	68-69	0.06939330128601995
1106	70-71	0.0756855386080062
1106	72-73	0.09374339387240127
1106	74-75	0.10426891192646792
1106	76-77	0.062804399739953
1106	78-79	0.11287175301329455
1106	80-81	0.08117894750578358
1106	82-83	0.0995183716702499
1106	84-85	0.0929216227872871
1106	86-87	0.028937467538128203
1106	88-89	0.038146365454345244
1106	90-91	0.05034742250228419
1106	92-93	0.11076616637926406
1106	94-95	0.08413898668028708
1106	96-97	0.023401950305668606
1106	98-99	0.04465819328135012
1106	100	0.09701452398903854
1107	1	0.011822045396549186
1107	2	-0.0018011580127179627
1107	3	0.017914275531033752
1107	4	0.030920408039918357
1107	5	0.003796349071144789
1107	6	0.04329300505661848
1107	7	0.06736009839537616
1107	8	0.003896379958952423
1107	9	0.045135667609265795
1107	10-11	0.043896170130793166
1107	12-13	0.07049945090838605
1107	14-15	0.08353490863259339
1107	16-17	0.017061984279749254
1107	18-19	0.05022298819753246
1107	20-21	0.03531135095208526
1107	22-23	0.06848505740455835
1107	24-25	0.0691880161126619
1107	26-27	0.045031938509872305
1107	28-29	0.08743161304690261
1107	30-31	0.07323216003029387
1107	32-33	0.013642533369292664
1107	34-35	-0.035727856295089566
1107	36-37	0.010683314010051959
1107	38-39	-0.004872120108473155
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2216	28-29	-0.3130165562790026
2216	30-31	-0.3090330591702113
2216	32-33	-0.31046309610015754
2216	34-35	-0.3555998561151483
2216	36-37	-0.3054490947615065
2216	38-39	-0.3425892761812932
2216	40-41	-0.27682519530436167
2216	42-43	-0.3232585966774053
2216	44-45	-0.2785857681579529
2216	46-47	-0.2418143444346228
2216	48-49	-0.30542947838188184
2216	50-51	-0.32209050623100666
2216	52-53	-0.3101990700975037
2216	54-55	-0.257073766203618
2216	56-57	-0.2929918138104739
2216	58-59	-0.2920896948394329
2216	60-61	-0.31883239759753934
2216	62-63	-0.404572246762676
2216	64-65	-0.47927515427648615
2216	66-67	-0.42026254641211125
2216	68-69	-0.4083839970945604
2216	70-71	-0.4321599989568554
2216	72-73	-0.4158933320510414
2216	74-75	-0.5581912356746486
2216	76-77	-0.4487363004810945
2216	78-79	-0.48478736867051
2216	80-81	-0.43238671556562025
2216	82-83	-0.4355209139280234
2216	84-85	-0.5245815081503764
2216	86-87	-0.48270655280567354
2216	88-89	-0.6373206503593067
2216	90-91	-0.5527239805941662
2216	92-93	-0.5426836560997685
2216	94-95	-0.4335363991460639
2216	96-97	-0.5803716457431563
2216	98-99	-0.5983356426184727
2216	100	-0.6374727092988763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31.0
3	14.0
4	17.0
5	36.0
6	80.0
7	218.0
8	519.0
9	1049.0
10	1883.0
11	3008.0
12	4608.0
13	6977.0
14	8415.0
15	10812.0
16	12718.0
17	17680.0
18	19629.0
19	10466.0
20	7676.0
21	7224.0
22	7922.0
23	8841.0
24	10458.0
25	12700.0
26	16531.0
27	21327.0
28	27915.0
29	36275.0
30	46199.0
31	59965.0
32	76345.0
33	100386.0
34	139176.0
35	207841.0
36	372330.0
37	863810.0
38	1686166.0
39	819680.0
40	1998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9772834306243	20.29962979076662	13.879831236979513	23.843255541629574
2	15.327418205001381	20.976840739370832	40.201253247242605	23.494487808385184
3	17.640679313600316	26.26459614715725	30.721009162221662	25.37371537702078
4	12.008436736573854	17.591542005235045	39.86606475452406	30.533956503667035
5	12.266775950664927	38.24358089730689	36.48774940493653	13.001893747091653
6	29.794003695896105	39.32528076755734	18.094574153182233	12.786141383364319
7	26.10876175353889	33.071955151574066	23.621748030050174	17.19753506483687
8	24.652753699081796	36.185403114636486	21.154226289118476	18.00761689716325
9	24.986254907996997	16.283305519100008	21.71759101735284	37.01284855555016
10-11	23.314523574380605	26.898178537993385	29.83640899770496	19.950888889921053
12-13	24.463509346122482	24.34934677057848	29.733923103096295	21.45322078020275
14-15	21.60132877877134	26.170577448869192	27.132444946026197	25.09564882633327
16-17	20.75026180527089	29.630242923966566	27.61369153803238	22.00580373273017
18-19	20.641456517883395	28.329249932435395	30.11733017520566	20.91196337447555
20-21	21.92376436470286	27.60717959430965	29.60862723079627	20.860428810191223
22-23	21.40976805052153	27.462543888500928	29.682695792266767	21.44499226871077
24-25	21.334136984762488	27.700050066187824	29.606464641227426	21.359348307822263
26-27	21.112502365564637	27.59967992506237	29.706533883959008	21.581283825413987
28-29	21.15637894176519	27.607038508986946	29.791874171578105	21.44470837766976
30-31	21.449070663050126	28.084886350883043	29.31740967139517	21.148633314671667
32-33	21.128415344815483	27.667957463125887	29.762860707399668	21.440766484658965
34-35	21.262766193014578	27.49899814751805	29.804144590806718	21.43409106866065
36-37	21.605610373898905	27.836419903109256	29.113919538553766	21.444050184438073
38-39	21.456752917794088	28.017304233704373	29.228352155198024	21.29759069330352
40-41	21.333662711713732	27.76355140176821	29.34564779290381	21.55713809361425
42-43	21.351750136370757	27.618140280950758	29.21602747938236	21.814082103296123
44-45	21.525894241103497	27.766490059787102	29.12301452281037	21.58460117629903
46-47	21.584169110538625	27.986335920327072	28.84870677317088	21.580788195963425
48-49	21.61164849290062	27.900819304698178	28.65904070599545	21.828491496405753
50-51	21.64975669296867	27.88869986011871	28.506262253114922	21.955281193797695
52-53	21.71556030827892	27.806855803453285	28.52430099861199	21.953282889655807
54-55	21.771318394659666	28.252175181062555	28.302899701334542	21.67360672294323
56-57	21.56515821708064	27.90738670425639	28.44079348876899	22.086661589893982
58-59	21.42762088389853	27.5843527384868	28.804603660677152	22.183422716937518
60-61	21.695264019183718	27.959277802081477	28.42768029294059	21.91777788579422
62-63	21.583434598745928	27.92471254124878	28.433826428382396	22.058026431622892
64-65	21.54236674821908	27.677225273686656	28.49802059873513	22.28238737935914
66-67	21.439969478871387	27.77910938059154	28.379562034334327	22.401359106202747
68-69	21.91712549454089	27.881104738486744	28.284493049072307	21.917276717900062
70-71	21.803414789268523	27.727826164352663	28.329477865698376	22.139281180680435
72-73	21.65060924723299	27.859955769275224	28.3681748766286	22.121260106863193
74-75	21.7151671913032	27.869379438266257	28.237597158213397	22.17785621221715
76-77	21.716705282544	27.869181289392248	28.138099018670644	22.27601440939311
78-79	21.86048596596402	27.951727452918334	28.16665856543366	22.02112801568399
80-81	21.799003008257856	27.66848674368239	28.326263657328642	22.20624659073111
82-83	21.58420555812215	27.776417369107413	28.410982303753606	22.22839476901683
84-85	21.561809707437472	27.703937739323926	28.25611778112629	22.478134772112316
86-87	21.67675203285272	27.84120595350754	28.14769335662036	22.334348657019383
88-89	21.434414484655957	27.691965687550084	28.257801590925368	22.61581823686859
90-91	21.360810443692753	27.603067518791224	28.377184478341956	22.658937559174067
92-93	21.502563949124355	27.742768818775193	28.186146821881554	22.5685204102189
94-95	21.25379747553278	27.503665829714315	28.37254443417682	22.86999226057609
96-97	21.24440838127384	27.50931130829917	28.35104212877555	22.895238181651443
98-99	21.229857205337012	27.560567860382967	28.225420640821252	22.984154293458765
100	21.2941881754403	27.451665343465276	28.19099466938868	23.063151811705744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	19.5
2	72.0
3	128.0
4	93.5
5	69.5
6	175.0
7	400.5
8	684.5
9	828.5
10	726.0
11	644.5
12	703.5
13	873.5
14	1050.5
15	1229.0
16	1504.0
17	1765.5
18	2141.5
19	2625.0
20	3217.0
21	4155.5
22	5578.0
23	7716.5
24	10508.5
25	14356.5
26	20244.0
27	28469.0
28	39219.5
29	51837.5
30	65874.5
31	82171.5
32	99509.5
33	117686.0
34	135286.5
35	149107.0
36	160838.0
37	172456.5
38	180569.0
39	183629.0
40	185700.0
41	187005.5
42	186800.5
43	185962.5
44	181629.5
45	176299.0
46	170711.5
47	163025.5
48	156218.5
49	151073.0
50	144945.5
51	136995.0
52	126084.0
53	116440.5
54	110060.5
55	103757.0
56	95006.0
57	85292.0
58	76620.0
59	66557.5
60	56716.5
61	46745.5
62	38254.0
63	31258.0
64	24741.0
65	19539.0
66	15177.0
67	11274.5
68	8540.0
69	6825.0
70	5047.5
71	3442.5
72	2407.5
73	1710.5
74	1161.0
75	714.5
76	412.5
77	264.5
78	165.5
79	81.5
80	48.5
81	24.5
82	8.5
83	3.5
84	1.5
85	1.5
86	1.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04497804565854923
2	0.24111429759609412
3	0.0029164438827589557
4	0.002613997850472842
5	1.5122301614305697E-4
6	1.5122301614305697E-4
7	0.0
8	1.2961972812262026E-4
9	0.0
10-11	7.237101486846298E-4
12-13	0.0
14-15	2.8084274426567725E-4
16-17	1.6202466015327534E-4
18-19	4.7527233644960765E-4
20-21	1.0801644010218355E-5
22-23	1.6202466015327534E-4
24-25	0.0011341726210729272
26-27	1.944295921839304E-4
28-29	3.996608283780791E-4
30-31	1.4042137213283863E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	7.561150807152848E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.0801644010218355E-5
68-69	2.2683452421458548E-4
70-71	2.160328802043671E-5
72-73	3.4565260832698737E-4
74-75	1.2961972812262026E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.1881808411240191E-4
84-85	0.0
86-87	5.076772684802628E-4
88-89	0.0023655600382378197
90-91	0.002214337022094763
92-93	6.589002846233197E-4
94-95	5.940904205620095E-4
96-97	2.2683452421458548E-4
98-99	1.1881808411240191E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4628925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.959610323021934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21841531162777	39.29395158338807
2	16.183046088821595	18.111939059399216
3	6.005138575210875	10.081356438136432
4	2.870388032047189	6.42503182996906
5	1.5011756183407658	4.200260131438538
6	0.89887512237691	3.018042094636233
7	0.5515105102009089	2.1603619267925716
8	0.3907027677611803	1.749085970883343
9	0.24896051993253113	1.2538560313117242
>10	1.0673442129196462	9.865579830084108
>50	0.044886527311059916	1.7199716242467984
>100	0.019093467908514582	1.751030824182161
>500	3.088305498875519E-4	0.10686777493902021
>1k	1.5441499116359832E-4	0.26266488059274645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4935	0.10661222638085517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.160328802043671E-5	0.0
5	0.0	0.0	0.0	2.160328802043671E-5	0.0
6	0.0	0.0	0.0	2.160328802043671E-5	0.0
7	0.0	0.0	0.0	2.160328802043671E-5	0.0
8	0.0	0.0	0.0	2.160328802043671E-5	0.0
9	0.0	0.0	0.0	1.0801644010218356E-4	0.0
10-11	0.0	0.0	0.0	2.2683452421458545E-4	0.0
12-13	0.0	0.0	0.0	3.3485096431676905E-4	0.0
14-15	0.0	0.0	0.0	3.4565260832698737E-4	0.0
16-17	0.0	0.0	0.0	3.6725589634742405E-4	0.0
18-19	0.0	0.0	0.0	3.780575403576424E-4	0.0
20-21	0.0	0.0	0.0	4.104624723882975E-4	0.0
22-23	0.0	0.0	0.0	4.2126411639851585E-4	0.0
24-25	0.0	0.0	0.0	4.968756244700444E-4	0.0
26-27	0.0	0.0	0.0	6.156937085824462E-4	0.0
28-29	0.0	0.0	0.0	0.0011449742650831455	0.0
30-31	0.0	0.0	0.0	0.004223442807995377	0.0
32-33	0.0	0.0	0.0	0.01188180841124019	0.0
34-35	0.0	0.0	0.0	0.023796021754511038	0.0
36-37	0.0	0.0	0.0	0.04453517825413028	0.0
38-39	0.0	0.0	0.0	0.07918685223891075	0.0
40-41	0.0	0.0	0.0	0.11926095151682087	0.0
42-43	0.0	0.0	0.0	0.161959850289214	0.0
44-45	0.0	0.0	0.0	0.21389415469034387	0.0
46-47	0.0	0.0	0.0	0.27773187079073436	0.0
48-49	0.0	0.0	0.0	0.34680838423608074	0.0
50-51	0.0	0.0	0.0	0.4223550824435479	0.0
52-53	0.0	0.0	0.0	0.49968405191270115	0.0
54-55	0.0	0.0	0.0	0.5857407497421108	0.0
56-57	0.0	0.0	0.0	0.6896417634764012	0.0
58-59	0.0	0.0	0.0	0.8050681313695944	0.0
60-61	0.0	0.0	0.0	0.926683841280643	0.0
62-63	0.0	0.0	0.0	1.0561955529631608	0.0
64-65	0.0	0.0	0.0	1.1965089086558973	0.0
66-67	0.0	0.0	0.0	1.3550122328618417	0.0
68-69	0.0	0.0	0.0	1.5370631410100617	0.0
70-71	0.0	0.0	0.0	1.731633154566125	0.0
72-73	0.0	0.0	0.0	1.9374476795368256	1.0801644010218355E-5
74-75	0.0	0.0	0.0	2.1571531187046666	2.160328802043671E-5
76-77	0.0	0.0	0.0	2.3918080331826506	2.160328802043671E-5
78-79	0.0	0.0	0.0	2.6595915898399736	2.160328802043671E-5
80-81	0.0	0.0	0.0	2.95324508390177	2.160328802043671E-5
82-83	0.0	0.0	2.160328802043671E-5	3.2567712805889055	2.160328802043671E-5
84-85	0.0	0.0	2.160328802043671E-5	3.5641536641876894	2.160328802043671E-5
86-87	0.0	0.0	2.160328802043671E-5	3.894824392272504	2.160328802043671E-5
88	0.0	0.0	2.160328802043671E-5	4.163299254146481	2.160328802043671E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5295	0.0	50.30573	1
GTATCAA	9840	0.0	44.126503	1
TCAACGC	11340	0.0	37.30101	4
ATCAACG	11460	0.0	36.99245	3
CAACGCA	11685	0.0	36.158302	5
TATCAAC	12300	0.0	34.59291	2
AACGCAG	12255	0.0	34.553223	6
ACGCAGA	13940	0.0	30.140596	7
CGCAGAG	14150	0.0	29.361141	8
GTACATG	11195	0.0	27.28889	1
TACATGG	11110	0.0	26.465187	2
GCAGAGT	16290	0.0	25.244343	9
ACATGGG	11555	0.0	24.445318	3
AGAGTAC	15230	0.0	20.443647	10-11
GAGTACT	11150	0.0	20.18974	12-13
CAGAGTA	16455	0.0	19.664297	10-11
CATGGGG	7990	0.0	18.999706	4
AGTACTT	11965	0.0	18.519918	12-13
GTACTTT	12440	0.0	18.020647	14-15
GTGGTAT	2865	0.0	17.936466	1
>>END_MODULE