FastQCFastQC Report
Wed 25 May 2016
SRR1033054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757633
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.036.487021
GTATCAA33800.032.9699821
ATCAACG33200.032.0032653
TCAACGC33150.031.7678954
CAACGCA34750.030.574675
AACGCAG38250.027.769936
GTACATG76200.027.5828781
CCGATAG1057.410617E-526.864943
TACATGG75100.026.7307952
TATCAAC42100.025.5727422
ACATGGG75600.025.4346333
ACGCAGA44300.023.4469497
CGCAGAG43900.023.044578
CATGGGG52300.020.405544
GAGTACA54350.019.0331271
GTCTAGC5650.017.4766371
GCAGAGT56450.016.757679
AGTACAT57300.016.5737462
TATACTG17950.016.5000115
GTATAGA12800.015.7959391
Produced by FastQC (version 0.11.5)