##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033054_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2757633
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664511920186623	33.0	31.0	34.0	30.0	34.0
2	31.77639083953521	34.0	31.0	34.0	30.0	34.0
3	31.880625159330485	34.0	31.0	34.0	30.0	34.0
4	35.22386952868638	37.0	35.0	37.0	33.0	37.0
5	35.25160200795392	37.0	35.0	37.0	33.0	37.0
6	35.35300527662673	37.0	36.0	37.0	33.0	37.0
7	35.26506246480224	37.0	36.0	37.0	33.0	37.0
8	35.259483767419376	37.0	36.0	37.0	33.0	37.0
9	36.89239721166667	39.0	38.0	39.0	34.0	39.0
10-11	36.88234076108024	39.0	38.0	39.0	34.0	39.0
12-13	36.8085822152549	39.0	38.0	39.0	33.0	39.0
14-15	38.217193694737475	41.0	39.0	41.0	34.0	41.0
16-17	38.22681353900247	41.0	39.0	41.0	34.0	41.0
18-19	38.24284957425444	41.0	39.0	41.0	34.0	41.0
20-21	38.2697893447025	41.0	39.0	41.0	34.0	41.0
22-23	38.24170819684853	40.5	39.0	41.0	34.0	41.0
24-25	38.259692098259634	40.5	39.0	41.0	34.0	41.0
26-27	38.17578318072057	40.0	38.0	41.0	33.0	41.0
28-29	38.18630579195999	40.0	38.5	41.0	33.0	41.0
30-31	38.33404173071616	41.0	39.0	41.0	34.0	41.0
32-33	38.32340851737704	41.0	39.0	41.0	33.5	41.0
34-35	38.28310511224663	41.0	39.0	41.0	33.0	41.0
36-37	38.21570383006005	41.0	38.0	41.0	33.0	41.0
38-39	38.13236007111897	41.0	38.0	41.0	33.0	41.0
40-41	38.06994241075589	40.5	38.0	41.0	33.0	41.0
42-43	37.96244514770457	40.0	38.0	41.0	33.0	41.0
44-45	37.84518607080783	40.0	38.0	41.0	33.0	41.0
46-47	37.7293733430083	40.0	38.0	41.0	32.0	41.0
48-49	37.62401904096738	40.0	38.0	41.0	32.0	41.0
50-51	37.515315671084586	40.0	38.0	41.0	32.0	41.0
52-53	37.37261448495866	40.0	37.0	41.0	31.5	41.0
54-55	37.222791611501606	40.0	37.0	41.0	31.0	41.0
56-57	37.065983762161245	40.0	37.0	41.0	31.0	41.0
58-59	36.8852553983797	40.0	36.0	41.0	31.0	41.0
60-61	36.675747099051975	39.5	36.0	41.0	31.0	41.0
62-63	36.43523975090231	39.0	35.0	41.0	30.0	41.0
64-65	36.169480130242135	39.0	35.0	41.0	30.0	41.0
66-67	35.881999707720354	38.5	35.0	40.5	30.0	41.0
68-69	35.57867870742771	38.0	35.0	40.0	29.5	41.0
70-71	35.25049743747627	37.0	35.0	40.0	29.0	41.0
72-73	34.9044756862135	37.0	34.5	39.5	29.0	41.0
74-75	34.553426253602275	36.0	34.0	39.0	29.0	41.0
76-77	34.18670142111006	36.0	34.0	39.0	29.0	40.0
78-79	33.796943066753265	35.0	34.0	37.5	28.0	39.5
80-81	33.43718489733769	35.0	34.0	37.0	28.0	39.0
82-83	33.194620712763445	35.0	34.0	37.0	28.0	39.0
84-85	32.95354675549647	35.0	34.0	36.0	28.0	38.0
86-87	32.75596353829534	35.0	34.0	36.0	28.0	37.0
88-89	32.52263680482501	35.0	34.0	36.0	27.5	37.0
90-91	32.297988165937966	35.0	34.0	35.0	27.0	36.5
92-93	32.073511776222574	35.0	33.0	35.0	27.0	36.0
94-95	31.871888862658665	35.0	33.0	35.0	26.0	36.0
96-97	31.694286005425667	35.0	33.0	35.0	26.0	36.0
98-99	31.52078376636775	35.0	33.0	35.0	25.5	35.5
100	31.388423332618952	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09161531769337827
1101	2	0.1646080922878319
1101	3	0.20374532549181268
1101	4	0.10653671204934767
1101	5	0.024555557683349605
1101	6	0.11127601611625693
1101	7	0.18154490027545478
1101	8	0.16603507246684757
1101	9	0.31999213023803463
1101	10-11	0.31523551903264746
1101	12-13	0.2569779431190966
1101	14-15	0.3199653299488361
1101	16-17	0.322698642324859
1101	18-19	0.3267833418967854
1101	20-21	0.3077098399927536
1101	22-23	0.33372233277933105
1101	24-25	0.31907717566596716
1101	26-27	0.23358551784169634
1101	28-29	0.23901930661282478
1101	30-31	0.31814750254169155
1101	32-33	0.29416901669547
1101	34-35	0.28884635904299216
1101	36-37	0.2854078584854136
1101	38-39	0.242640022604256
1101	40-41	0.2558159072402191
1101	42-43	0.25078201265239386
1101	44-45	0.25980171009280184
1101	46-47	0.31155805968840156
1101	48-49	0.2650797734306849
1101	50-51	0.24960900174374956
1101	52-53	0.16863448271888615
1101	54-55	0.18102948330511026
1101	56-57	0.32016037256772023
1101	58-59	0.3386324531126448
1101	60-61	0.34059087440289915
1101	62-63	0.23174113857871248
1101	64-65	0.23166805267670298
1101	66-67	0.2025108739755055
1101	68-69	0.22174656220041555
1101	70-71	0.11777578536472788
1101	72-73	0.14045185698703477
1101	74-75	0.13307696123537482
1101	76-77	0.12438873934584649
1101	78-79	0.08237451798409268
1101	80-81	0.1796715193131888
1101	82-83	0.12142666890137832
1101	84-85	0.14120766791749162
1101	86-87	0.11482724768962527
1101	88-89	0.2034625160216521
1101	90-91	0.18782541518314844
1101	92-93	0.21319392185635877
1101	94-95	0.29788030629678275
1101	96-97	0.31873774657848486
1101	98-99	0.2580642961577695
1101	100	0.22694952943754743
1102	1	0.16876705682842186
1102	2	0.3140248440968598
1102	3	0.2826227922639113
1102	4	0.16053691588366803
1102	5	0.13600369012969793
1102	6	0.23589429307158838
1102	7	0.32443955060931273
1102	8	0.375251681651541
1102	9	0.4275456990472648
1102	10-11	0.31658890582350097
1102	12-13	0.3604040907055861
1102	14-15	0.49045957297216347
1102	16-17	0.4551545430077084
1102	18-19	0.338063430402066
1102	20-21	0.42895578550273683
1102	22-23	0.41310621443842876
1102	24-25	0.3821277129992069
1102	26-27	0.2871398900506321
1102	28-29	0.2556304610059854
1102	30-31	0.31215975737160306
1102	32-33	0.3603241104421002
1102	34-35	0.2905795103678912
1102	36-37	0.32749017084405807
1102	38-39	0.3510253801969583
1102	40-41	0.24026324389708265
1102	42-43	0.27235051180355896
1102	44-45	0.20582064370503161
1102	46-47	0.18894358157216828
1102	48-49	0.20480666528105473
1102	50-51	0.25569340407569996
1102	52-53	0.23205042217502125
1102	54-55	0.2918705893764013
1102	56-57	0.2569032403435614
1102	58-59	0.25110176418397145
1102	60-61	0.21269643819953643
1102	62-63	0.18812227205572185
1102	64-65	0.19456591571405824
1102	66-67	0.1840401790392292
1102	68-69	0.2301958754234832
1102	70-71	0.14940509757609277
1102	72-73	0.2052914284813241
1102	74-75	0.1798422776463795
1102	76-77	0.16304641869952974
1102	78-79	0.11373217544722536
1102	80-81	0.17226118664393653
1102	82-83	0.07155097650259279
1102	84-85	0.1311885991120718
1102	86-87	0.09941764888363025
1102	88-89	0.15192264917650533
1102	90-91	0.17977194493362703
1102	92-93	0.21419059858094158
1102	94-95	0.27263204549150544
1102	96-97	0.2432876733786813
1102	98-99	0.27074574563767584
1102	100	0.22011697605153202
1103	1	-0.006978149809174994
1103	2	0.07002670181515214
1103	3	0.03458383417162736
1103	4	0.05775678747028934
1103	5	0.024540949800964995
1103	6	0.11415553959827918
1103	7	0.11029938057937727
1103	8	0.12306849485907634
1103	9	0.18917905993117756
1103	10-11	0.17011236794420626
1103	12-13	0.214623125919033
1103	14-15	0.24783747202361894
1103	16-17	0.332710575046562
1103	18-19	0.26232263023911884
1103	20-21	0.22067183456186967
1103	22-23	0.16637948592733665
1103	24-25	0.185603478781303
1103	26-27	0.13119293115018849
1103	28-29	0.192789490390318
1103	30-31	0.21128737537639353
1103	32-33	0.18266148028236984
1103	34-35	0.15944128939954538
1103	36-37	0.17040309911928375
1103	38-39	0.15290317401436937
1103	40-41	0.1898790985785439
1103	42-43	0.21286478238953066
1103	44-45	0.20237941554270833
1103	46-47	0.22843891381571524
1103	48-49	0.2021082173385622
1103	50-51	0.1412430453693787
1103	52-53	0.17483770521774034
1103	54-55	0.1582084297167512
1103	56-57	0.25121920942910236
1103	58-59	0.13494423578630688
1103	60-61	0.12806348646785892
1103	62-63	0.19698267843838835
1103	64-65	0.1987576372122959
1103	66-67	0.1777531324249466
1103	68-69	0.15842283072162644
1103	70-71	0.14574051042741587
1103	72-73	0.16403543465811055
1103	74-75	0.25299561303702234
1103	76-77	0.15678529672538843
1103	78-79	0.1000025431621765
1103	80-81	0.22550168453129515
1103	82-83	0.1128679253681284
1103	84-85	0.128327425845697
1103	86-87	0.026727957470399133
1103	88-89	-0.021028838251517357
1103	90-91	0.025599716907926506
1103	92-93	0.0883998524205225
1103	94-95	0.14032732284337612
1103	96-97	0.0827363769788434
1103	98-99	0.029403129235859637
1103	100	0.024971282787731752
1104	1	-0.006943550044354652
1104	2	0.08956733089791413
1104	3	0.09524909840364515
1104	4	0.07377304606453805
1104	5	0.039466903901491435
1104	6	0.144017951299368
1104	7	0.14939299580377252
1104	8	0.24213239547592025
1104	9	0.2201300598678202
1104	10-11	0.21647522899601057
1104	12-13	0.28379619085338703
1104	14-15	0.22621725290962758
1104	16-17	0.22382206022567175
1104	18-19	0.21152485508637398
1104	20-21	0.2226483461270803
1104	22-23	0.24117511562005234
1104	24-25	0.20398927206294104
1104	26-27	0.11286930073965351
1104	28-29	0.13510921102741946
1104	30-31	0.17064706828425358
1104	32-33	0.12614276445264494
1104	34-35	0.14121507380305331
1104	36-37	0.1487631430362768
1104	38-39	0.15776241578661399
1104	40-41	0.26401537620540694
1104	42-43	0.21514417919754436
1104	44-45	0.18754432014998912
1104	46-47	0.19440630367467548
1104	48-49	0.18838947923866556
1104	50-51	0.17465426707541098
1104	52-53	0.15478037944866685
1104	54-55	0.1481178613998253
1104	56-57	0.12964837049533173
1104	58-59	0.16409879396550764
1104	60-61	0.1650769624584001
1104	62-63	0.16334250789175542
1104	64-65	0.14357636705356214
1104	66-67	0.14645469318327287
1104	68-69	0.14811855393668338
1104	70-71	0.12693965726860768
1104	72-73	0.15480031806615813
1104	74-75	0.20109555390849465
1104	76-77	0.14315436239374435
1104	78-79	0.24210007015084756
1104	80-81	0.1970277600193242
1104	82-83	0.19796319794871664
1104	84-85	0.12441054711245414
1104	86-87	0.1060131125992001
1104	88-89	0.14781108037588098
1104	90-91	0.194690483830243
1104	92-93	0.1626806733109305
1104	94-95	0.22793828665758298
1104	96-97	0.20443233113360648
1104	98-99	0.16688529473903202
1104	100	0.1562969556141418
1105	1	0.11118118738417948
1105	2	0.17394886256697006
1105	3	0.1548596054372986
1105	4	0.21776378006430974
1105	5	0.1356201177762273
1105	6	0.14603378256474997
1105	7	0.19712196464485743
1105	8	0.18953310105926846
1105	9	0.31882933627957044
1105	10-11	0.27720579087937125
1105	12-13	0.3241418784187857
1105	14-15	0.35056428170963727
1105	16-17	0.27346659391179884
1105	18-19	0.2915144957450977
1105	20-21	0.28116159799476037
1105	22-23	0.27426897073019063
1105	24-25	0.279614811883782
1105	26-27	0.13861001321183863
1105	28-29	0.16010095878137776
1105	30-31	0.25097178927352815
1105	32-33	0.19880336686008349
1105	34-35	0.23259744570079732
1105	36-37	0.31804538028868024
1105	38-39	0.2590575291522512
1105	40-41	0.2541862009603193
1105	42-43	0.32339423986623217
1105	44-45	0.2622757144338266
1105	46-47	0.1967111592571058
1105	48-49	0.2201994740459412
1105	50-51	0.24497472168609846
1105	52-53	0.26556919631795495
1105	54-55	0.22734438131351453
1105	56-57	0.2764650910462336
1105	58-59	0.2074865268875925
1105	60-61	0.18069044030062997
1105	62-63	0.22187148142976554
1105	64-65	0.2045037107566685
1105	66-67	0.20875579122125032
1105	68-69	0.22351844538540178
1105	70-71	0.19169242258647046
1105	72-73	0.19367306125626982
1105	74-75	0.23266192806076447
1105	76-77	0.24035531795817633
1105	78-79	0.2880958063816692
1105	80-81	0.27377762759198276
1105	82-83	0.2614300480566172
1105	84-85	0.25687573111470385
1105	86-87	0.19376599664503402
1105	88-89	0.23096827881337134
1105	90-91	0.14252223064481484
1105	92-93	0.22160829882987088
1105	94-95	0.28297248292143706
1105	96-97	0.3325557573356974
1105	98-99	0.36469613368910814
1105	100	0.38252892135786
1106	1	-0.045996047977769905
1106	2	-0.031099910316200408
1106	3	0.03216907940282354
1106	4	0.024311991230355545
1106	5	0.0516684007034911
1106	6	0.08259550463602494
1106	7	0.07964699446287682
1106	8	0.13351394328851995
1106	9	0.09361454752974652
1106	10-11	0.11553475667649593
1106	12-13	0.11858675590506351
1106	14-15	0.1629135831513011
1106	16-17	0.12934173434108232
1106	18-19	0.1376740333059061
1106	20-21	0.10810830662936866
1106	22-23	0.10838165563905022
1106	24-25	0.06528681857489715
1106	26-27	0.11278234654022157
1106	28-29	0.17972301224100562
1106	30-31	0.154301324211346
1106	32-33	0.12920166636666153
1106	34-35	0.16670641882251402
1106	36-37	0.12440150646963843
1106	38-39	0.17775466711690058
1106	40-41	0.22817189325812137
1106	42-43	0.11570768544400778
1106	44-45	0.17726780832719413
1106	46-47	0.2107037925968669
1106	48-49	0.15675197851246025
1106	50-51	0.11180217059953179
1106	52-53	0.15520483491856396
1106	54-55	0.19967474026807253
1106	56-57	0.18221452561732576
1106	58-59	0.2758598114233024
1106	60-61	0.24121280010643176
1106	62-63	0.24622204240453271
1106	64-65	0.2437144115915757
1106	66-67	0.1619070366495663
1106	68-69	0.27063129361290095
1106	70-71	0.13377239675243402
1106	72-73	0.2065663869680705
1106	74-75	0.2372699880828364
1106	76-77	0.21947663895846858
1106	78-79	0.1763653217212493
1106	80-81	0.20128630080129284
1106	82-83	0.1649967661934042
1106	84-85	0.18626640413595652
1106	86-87	0.19251042277799257
1106	88-89	0.17515042363245925
1106	90-91	0.18308099070994643
1106	92-93	0.25327590581595416
1106	94-95	0.22045509938494234
1106	96-97	0.1646160095392588
1106	98-99	0.19327912182623663
1106	100	0.23228245869101372
1107	1	-0.0189085026193041
1107	2	-0.0044907055277718655
1107	3	0.09228558941500609
1107	4	0.03348130009217698
1107	5	0.0378144751430014
1107	6	0.056069484410791404
1107	7	0.1063434902456919
1107	8	0.08547092760054653
1107	9	0.14632411161917247
1107	10-11	0.1506035616909358
1107	12-13	0.09805993832821969
1107	14-15	0.13730452901022971
1107	16-17	0.12865860018139585
1107	18-19	0.12446904119963875
1107	20-21	0.11814348583872203
1107	22-23	0.12418842388240137
1107	24-25	0.07392428593463762
1107	26-27	0.08174611561518219
1107	28-29	0.11627911970091276
1107	30-31	0.16435562765737188
1107	32-33	0.14191769984510216
1107	34-35	0.1492858817857865
1107	36-37	0.2567601138309925
1107	38-39	0.29083217213378987
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2216	44-45	-0.6485826279565217
2216	46-47	-0.5594441559466148
2216	48-49	-0.6780923041291729
2216	50-51	-0.6505531077069477
2216	52-53	-0.6817811388891144
2216	54-55	-0.6919048016070946
2216	56-57	-0.6888086451588578
2216	58-59	-0.6922651842071588
2216	60-61	-0.5941145416437692
2216	62-63	-0.7227774763314159
2216	64-65	-0.7800034316274491
2216	66-67	-0.7540082257319227
2216	68-69	-0.7830728240384914
2216	70-71	-0.7394583154144385
2216	72-73	-0.6680320636526744
2216	74-75	-0.7075820076324604
2216	76-77	-0.6615559076803947
2216	78-79	-0.70060609890335
2216	80-81	-0.6115617866039713
2216	82-83	-0.7310867609902729
2216	84-85	-0.6164633285915002
2216	86-87	-0.5173866014531896
2216	88-89	-0.6654019019764021
2216	90-91	-0.6665055227861387
2216	92-93	-0.7957863378570877
2216	94-95	-0.7076758048151675
2216	96-97	-0.6489468409573007
2216	98-99	-0.608247923153904
2216	100	-0.541458153400523
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28186.0
3	6024.0
4	3228.0
5	2695.0
6	2203.0
7	2457.0
8	2817.0
9	3287.0
10	3645.0
11	4078.0
12	4607.0
13	5303.0
14	5690.0
15	5706.0
16	5691.0
17	5623.0
18	5515.0
19	5602.0
20	5622.0
21	5901.0
22	6887.0
23	9775.0
24	15715.0
25	21179.0
26	22075.0
27	18577.0
28	19555.0
29	24547.0
30	29818.0
31	35884.0
32	46461.0
33	62457.0
34	89053.0
35	136171.0
36	242812.0
37	520947.0
38	909329.0
39	431450.0
40	1061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.804788885199926	20.072243297719673	13.38645447238989	23.736513344690515
2	15.061735113103952	20.23754459856305	41.18540123742307	23.515319050909927
3	18.082846059065044	25.604684508959608	30.07831945164048	26.234149980334877
4	11.841421496048818	16.7631962468138	39.92496095269615	31.47042130444123
5	12.373291851299102	38.45209634410538	36.13451996534614	13.040091839249374
6	30.298606811257876	38.89267154992883	18.071784098935055	12.736937539878241
7	26.847532727349275	32.88973384030418	22.93941466063296	17.32331877171358
8	24.295450379240936	37.60933918242224	20.631178886652425	17.464031551684396
9	24.377791982321515	17.624656668071392	21.487796494139534	36.509754855467556
10-11	23.278286014225134	27.68296693603481	29.323188044904207	19.71555900483585
12-13	23.837640171364775	24.969066599968745	29.354684896786722	21.83860833187976
14-15	21.084852783799757	27.175190690610023	26.32427059843382	25.415685927156407
16-17	20.8406313944034	30.620411248652058	26.898567943958152	21.640389412986398
18-19	20.77364923910777	29.577545053796676	28.820854340168346	20.82795136692721
20-21	21.986121283964525	28.747177108793192	28.361136981816625	20.905564625425658
22-23	21.48586876837167	28.48353558990629	28.524099986520863	21.506495655201178
24-25	21.443373941902692	28.61196675749566	28.42012867188669	21.52453062871496
26-27	21.23152647619719	28.572710434649455	28.482416923521527	21.71334616563183
28-29	21.291060604136312	28.55751523585411	28.52262562889288	21.628798531116704
30-31	21.491266111685913	29.054127861381346	28.210025581276348	21.244580445656393
32-33	21.14148820121192	28.686499817464078	28.728087524341145	21.443924456982852
34-35	21.217915109841734	28.47742385468977	28.751518136666665	21.55314289880183
36-37	21.562956522338563	28.85682860819202	28.204488628767216	21.375726240702207
38-39	21.350108117592605	28.956317299050703	28.380155862800528	21.313418720556168
40-41	21.119545132620356	28.67828211133574	28.67145924580843	21.53071351023548
42-43	21.11948982333939	28.551624703852475	28.610704514034996	21.718180958773143
44-45	21.289833386042616	28.73802723573098	28.569284948206096	21.402854430020305
46-47	21.293205501445474	28.89006472160709	28.462863646228225	21.353866130719208
48-49	21.237985037239966	28.63181525304433	28.55656778649969	21.57363192321601
50-51	21.294669206599885	28.600158470640384	28.462099951899972	21.643072370859766
52-53	21.329257290752622	28.602264322942183	28.513989985534984	21.554488400770207
54-55	21.449252065419962	29.002077757328394	28.31169335407946	21.23697682317218
56-57	21.26112229062987	28.70067623236731	28.40121511722528	21.636986359777538
58-59	21.097105206484436	28.377260613423427	28.74456862218947	21.781065557902675
60-61	21.375720737482197	28.773982930352915	28.363379596359795	21.486916735805092
62-63	21.21679189286823	28.760827601522855	28.407729085385757	21.61465142022316
64-65	21.222565067221268	28.525417550918284	28.538879765909865	21.713137615950583
66-67	21.097399100665648	28.550672468698927	28.412437550257348	21.93949088037808
68-69	21.450243747821656	28.720730802579773	28.370653448094114	21.458372001504454
70-71	21.40528745291241	28.504383472772833	28.440832007152306	21.64949706716245
72-73	21.166508017454497	28.613006768125963	28.562137426682565	21.65834778773697
74-75	21.215917898165163	28.59092116377696	28.44957459963599	21.743586338421885
76-77	21.225559977367066	28.62570956952616	28.34831280689205	21.800417646214726
78-79	21.364956579804506	28.679532998205726	28.381593414417743	21.573917007572028
80-81	21.280614627106413	28.49748385849239	28.498100604302056	21.72380091009914
82-83	21.105630009884848	28.562822121525404	28.642677329991578	21.688870538598167
84-85	21.089022975631302	28.49736487203463	28.51118670999444	21.902425442339627
86-87	21.334836179163215	28.59884899519254	28.30997319836259	21.756341627281657
88-89	21.087528056422293	28.499575004380556	28.426747116541673	21.986149822655477
90-91	21.073771404670204	28.41624939281712	28.542601474361557	21.96737772815112
92-93	21.218616538380264	28.535158818477235	28.398519514135085	21.84770512900742
94-95	21.005342685527044	28.29820365689638	28.597597197244042	22.098856460332534
96-97	21.020938823301073	28.292724513941224	28.582822492070964	22.103514170686736
98-99	21.01416151880439	28.41059480120327	28.49938544899952	22.075858230992814
100	21.113667713736938	28.31577694718427	28.391096347154228	22.17945899192457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	430.0
1	399.5
2	364.5
3	353.5
4	354.0
5	344.0
6	317.5
7	302.0
8	286.5
9	261.0
10	256.5
11	259.0
12	250.0
13	276.0
14	324.5
15	394.0
16	512.5
17	744.5
18	1025.0
19	1394.0
20	2080.0
21	3136.5
22	4815.0
23	7453.0
24	10608.0
25	14517.5
26	19964.5
27	27185.0
28	35763.5
29	44444.0
30	52610.5
31	60464.5
32	68696.0
33	77390.0
34	84448.5
35	90415.0
36	95034.5
37	98466.0
38	101179.0
39	102281.5
40	103568.5
41	103941.0
42	102727.5
43	101015.0
44	98222.0
45	95828.0
46	93840.0
47	90423.5
48	87064.5
49	84242.5
50	81097.5
51	77249.5
52	71223.5
53	66533.0
54	63678.0
55	59874.5
56	54962.5
57	49380.5
58	44153.5
59	39177.5
60	34221.0
61	29098.0
62	24769.5
63	20968.5
64	17520.0
65	13993.0
66	10770.5
67	7875.0
68	5636.5
69	4364.5
70	3110.0
71	2203.0
72	1683.5
73	1194.0
74	820.0
75	539.0
76	337.0
77	236.0
78	152.0
79	91.5
80	77.0
81	42.5
82	26.0
83	26.5
84	17.5
85	8.0
86	7.0
87	8.5
88	5.5
89	5.5
90	5.5
91	4.0
92	2.5
93	3.0
94	2.0
95	0.5
96	0.5
97	2.0
98	2.5
99	1.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0604866564912735
2	0.03981675589173759
3	0.05385053050931723
4	0.07256222999942342
5	0.08645095268297123
6	0.08597953389736777
7	0.08866299467695665
8	0.10110119802018616
9	0.09595185436205615
10-11	0.10120998697071001
12-13	0.10605109526902237
14-15	0.10548901902464904
16-17	0.07432098469955936
18-19	0.09705787535904886
20-21	0.08989593611622722
22-23	0.05517412940735769
24-25	0.02250118126668777
26-27	0.17926605897158904
28-29	0.065200844347308
30-31	0.07622479133372714
32-33	0.07292485983450299
34-35	0.020035298388146643
36-37	0.05082257138640276
38-39	0.07500998138621057
40-41	0.07950659134119732
42-43	0.07392209188097183
44-45	0.07076721231577951
46-47	0.07756652172352159
48-49	0.07464735155113099
50-51	0.06953427087650896
52-53	0.07321496370256665
54-55	0.06443932169364089
56-57	0.06706838799796783
58-59	0.06423987528434712
60-61	0.061230047653186626
62-63	0.06841011838776226
64-65	0.06423987528434712
66-67	0.0658898410339592
68-69	0.06576292059168135
70-71	0.05798451062922442
72-73	0.05629828189610438
74-75	0.05950755593655863
76-77	0.05339724321546776
78-79	0.04966215591414811
80-81	0.04460346971478801
82-83	0.050568730501847056
84-85	0.040813987938206425
86-87	0.03934533710613414
88-89	0.04112222329802406
90-91	0.03807613268335562
92-93	0.04008872826804727
94-95	0.038456894010189174
96-97	0.04172056252590537
98-99	0.04166616805064343
100	0.04971655038941005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2757633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.20808882226986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2556137741339	53.26618330195506
2	12.83521237709154	17.252601869845314
3	3.8401478750388547	7.742669984287866
4	1.529656822782669	4.11221246452675
5	0.7560375775225637	2.54059203315551
6	0.4774115082040369	1.9251549028890438
7	0.28082334891373356	1.3211520404013042
8	0.19377440190425152	1.0418565771730524
9	0.1422216855335126	0.8602602906410325
>10	0.6491135586053689	7.535030146006565
>50	0.030638493741513433	1.404640654496862
>100	0.009240616964746469	0.9522247025484895
>500	1.0795956334360957E-4	0.04542103207309288
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.626298350795773E-5	0.0
10-11	0.0	0.0	0.0	3.626298350795773E-5	0.0
12-13	0.0	0.0	0.0	5.43944752619366E-5	0.0
14-15	0.0	0.0	0.0	7.252596701591546E-5	0.0
16-17	0.0	0.0	0.0	1.087889505238732E-4	0.0
18-19	0.0	1.8131491753978866E-5	0.0	1.087889505238732E-4	0.0
20-21	0.0	3.626298350795773E-5	0.0	1.8131491753978866E-4	0.0
22-23	0.0	3.626298350795773E-5	0.0	2.538408845557041E-4	0.0
24-25	0.0	3.626298350795773E-5	0.0	2.538408845557041E-4	0.0
26-27	0.0	3.626298350795773E-5	0.0	3.2636685157161956E-4	0.0
28-29	0.0	3.626298350795773E-5	0.0	8.159171289290491E-4	0.0
30-31	0.0	3.626298350795773E-5	0.0	0.003136748073438344	0.0
32-33	0.0	3.626298350795773E-5	0.0	0.008684984550155875	0.0
34-35	0.0	3.626298350795773E-5	0.0	0.01729744313329584	0.0
36-37	0.0	3.626298350795773E-5	0.0	0.033289418860305196	0.0
38-39	0.0	3.626298350795773E-5	0.0	0.060740497375829205	0.0
40-41	0.0	3.626298350795773E-5	0.0	0.09375794385982472	0.0
42-43	0.0	7.252596701591546E-5	0.0	0.12715615167065378	0.0
44-45	0.0	7.252596701591546E-5	0.0	0.16508723241997758	0.0
46-47	0.0	7.252596701591546E-5	0.0	0.21264613529066412	0.0
48-49	0.0	7.252596701591546E-5	0.0	0.2675301608299582	0.0
50-51	0.0	1.087889505238732E-4	0.0	0.3289233919089306	0.0
52-53	0.0	1.8131491753978866E-4	0.0	0.3905160693971968	0.0
54-55	0.0	1.8131491753978866E-4	0.0	0.45849103198286356	0.0
56-57	0.0	1.8131491753978866E-4	0.0	0.540554163661372	0.0
58-59	0.0	1.8131491753978866E-4	0.0	0.6322995119365049	0.0
60-61	0.0	1.8131491753978866E-4	0.0	0.7370270083074868	0.0
62-63	0.0	1.8131491753978866E-4	0.0	0.8414462693186511	0.0
64-65	0.0	1.8131491753978866E-4	0.0	0.9548224872562809	0.0
66-67	0.0	1.8131491753978866E-4	0.0	1.0837736566105787	0.0
68-69	0.0	1.8131491753978866E-4	0.0	1.230185452523958	0.0
70-71	0.0	1.8131491753978866E-4	0.0	1.3874217490144627	0.0
72-73	0.0	1.8131491753978866E-4	0.0	1.5602692599051433	0.0
74-75	0.0	1.9944640929376754E-4	0.0	1.7340414768752768	0.0
76-77	0.0	2.175779010477464E-4	0.0	1.9234067767538319	0.0
78-79	0.0	2.175779010477464E-4	0.0	2.1410934667521024	0.0
80-81	0.0	2.175779010477464E-4	0.0	2.3832758021099982	0.0
82-83	0.0	2.175779010477464E-4	0.0	2.6380051297616474	0.0
84-85	0.0	2.175779010477464E-4	0.0	2.89079438779562	0.0
86-87	0.0	2.175779010477464E-4	0.0	3.1573273165791096	0.0
88	0.0	2.175779010477464E-4	0.0	3.3812693712325026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2165	0.0	36.48702	1
GTATCAA	3380	0.0	32.969982	1
ATCAACG	3320	0.0	32.003265	3
TCAACGC	3315	0.0	31.767895	4
CAACGCA	3475	0.0	30.57467	5
AACGCAG	3825	0.0	27.76993	6
GTACATG	7620	0.0	27.582878	1
CCGATAG	105	7.410617E-5	26.86494	3
TACATGG	7510	0.0	26.730795	2
TATCAAC	4210	0.0	25.572742	2
ACATGGG	7560	0.0	25.434633	3
ACGCAGA	4430	0.0	23.446949	7
CGCAGAG	4390	0.0	23.04457	8
CATGGGG	5230	0.0	20.40554	4
GAGTACA	5435	0.0	19.033127	1
GTCTAGC	565	0.0	17.476637	1
GCAGAGT	5645	0.0	16.75767	9
AGTACAT	5730	0.0	16.573746	2
TATACTG	1795	0.0	16.500011	5
GTATAGA	1280	0.0	15.795939	1
>>END_MODULE