FastQCFastQC Report
Wed 25 May 2016
SRR1033053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135084
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24231.7936987356015517No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18211.3480501021586568No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11530.8535429806638832No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8120.6011074590625092No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5690.42121938941695536No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4760.3523733380711261No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4650.3442302567291463No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4540.3360871753871665No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4490.3323857747771757No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4360.32276213319119956No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4200.31091765123922893No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4020.29759260904326196No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3590.2657605637973409No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3010.2228243167214474No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2990.22134375647745105No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2860.21172011489147494No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2630.19469367208551716No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2580.19099227147552633No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2540.18803115098753367No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC2010.14879630452163098No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1820.13473098220366586No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1690.12510734061768974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAT156.843654E-494.155911
GTTTAAG250.005191876656.493541
GTAACAA250.005191876656.493541
ATAGAAC250.005207156356.4516333
TACATGA602.5152985E-854.8835332
TACATAA451.5976202E-552.270032
TAACAAC1250.045.194831
GGAAACA1900.044.6001661
GAGAACA2200.042.7981381
GTACATA802.405559E-741.1932071
ACGTGGG500.001689094737.6344223
GAGTGTT906.128139E-736.5618977
GTACAAA1601.8189894E-1235.3084641
GCAGAAA1800.033.975523
TTGAGTG851.728091E-533.2191665
CATGAAA851.7324883E-533.206844
AGAACAA1850.033.0572622
AGCACCA600.004122051431.3736575
ACATAAA1052.032979E-631.3620193
GACCAAT600.004137055531.350399
Produced by FastQC (version 0.11.5)