##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033053_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135084
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.924876373219625	31.0	28.0	34.0	12.0	34.0
2	27.97905747534867	31.0	28.0	34.0	7.0	34.0
3	28.12380444760297	31.0	27.0	34.0	10.0	34.0
4	30.782957271031357	37.0	32.0	37.0	7.0	37.0
5	31.571163128127683	37.0	32.0	37.0	10.0	37.0
6	31.176238488644103	37.0	32.0	37.0	8.0	37.0
7	31.486341831749133	37.0	32.0	37.0	8.0	37.0
8	31.03708803411211	37.0	32.0	37.0	8.0	37.0
9	32.07670782624145	39.0	32.0	39.0	8.0	39.0
10-11	32.47490080246365	39.0	32.0	39.0	7.5	39.0
12-13	32.462367859998224	39.0	32.0	39.0	7.5	39.0
14-15	33.51665630274496	40.0	32.0	41.0	7.0	41.0
16-17	33.653585916910956	40.0	32.0	41.0	7.0	41.0
18-19	33.650820970655296	40.0	32.0	41.0	7.5	41.0
20-21	33.69904651920287	40.0	32.0	41.0	8.0	41.0
22-23	33.612855704598616	40.0	32.0	41.0	8.0	41.0
24-25	33.544290959699154	40.0	31.5	41.0	5.0	41.0
26-27	33.10843253086968	39.5	31.0	41.0	2.0	41.0
28-29	32.80506203547422	39.0	30.0	41.0	2.0	41.0
30-31	32.61924432205146	40.0	30.0	41.0	2.0	41.0
32-33	32.29711882976518	40.0	30.0	41.0	2.0	41.0
34-35	31.90435580823784	40.0	30.0	41.0	2.0	41.0
36-37	31.505503982707054	39.5	30.0	41.0	2.0	41.0
38-39	31.188534541470496	39.0	30.0	41.0	2.0	41.0
40-41	30.986697166207694	39.0	29.5	41.0	2.0	41.0
42-43	30.710887299754226	39.0	28.5	41.0	2.0	41.0
44-45	30.537543306387136	39.0	28.0	41.0	2.0	41.0
46-47	30.304355067957715	38.0	28.0	41.0	2.0	41.0
48-49	30.080808978117318	38.0	27.0	41.0	2.0	41.0
50-51	29.876399129430574	38.0	27.0	41.0	2.0	41.0
52-53	29.69377572473424	38.0	26.5	41.0	2.0	41.0
54-55	29.48836279648219	37.0	26.0	40.0	2.0	41.0
56-57	29.21748319564123	37.0	25.5	40.0	2.0	41.0
58-59	28.950001480560246	36.0	25.0	40.0	2.0	41.0
60-61	28.618426312516657	35.5	23.0	40.0	2.0	41.0
62-63	28.39236327026147	35.0	23.0	39.5	2.0	41.0
64-65	28.099086494329455	35.0	22.0	39.0	2.0	41.0
66-67	27.748034556276096	35.0	20.5	39.0	2.0	41.0
68-69	27.356670664179326	35.0	19.0	37.5	2.0	40.0
70-71	26.972354238843977	35.0	17.0	37.0	2.0	39.5
72-73	26.671219389416954	34.5	14.0	36.5	2.0	39.0
74-75	26.38721462201297	34.0	9.0	36.0	2.0	39.0
76-77	26.1168125018507	34.0	7.0	35.5	2.0	38.0
78-79	25.78140268277516	34.0	2.0	35.0	2.0	37.0
80-81	25.476803322377187	34.0	2.0	35.0	2.0	37.0
82-83	25.3310976873649	34.0	2.0	35.0	2.0	36.0
84-85	25.200941636315182	34.0	2.0	35.0	2.0	36.0
86-87	25.146601373959903	34.0	2.0	35.0	2.0	36.0
88-89	24.933067572769538	34.0	2.0	35.0	2.0	35.0
90-91	24.68987444849131	34.0	2.0	35.0	2.0	35.0
92-93	24.417355127180127	33.5	2.0	35.0	2.0	35.0
94-95	24.171944863936513	33.0	2.0	35.0	2.0	35.0
96-97	23.984468923040478	33.0	2.0	35.0	2.0	35.0
98-99	23.772967190784993	33.0	2.0	35.0	2.0	35.0
100	23.605712001421338	33.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5451654470946501
1101	2	0.4292648296180772
1101	3	0.7258339349295255
1101	4	0.6479833174671903
1101	5	0.457065466500719
1101	6	0.21594739875207836
1101	7	0.6590043692085565
1101	8	-0.00906220877351771
1101	9	0.6892095883599438
1101	10-11	0.31606087419176987
1101	12-13	0.5465865977897337
1101	14-15	0.5172612650768755
1101	16-17	0.604421618581199
1101	18-19	0.7752108987720874
1101	20-21	0.8214238331763042
1101	22-23	0.8526265368835055
1101	24-25	0.8424763984292767
1101	26-27	0.8361078185675339
1101	28-29	0.8631048440968527
1101	30-31	0.818134529868793
1101	32-33	0.6248230373442425
1101	34-35	0.6153061666895319
1101	36-37	0.5164270291108188
1101	38-39	0.6094141205756287
1101	40-41	0.36797043583620237
1101	42-43	0.15044933243142822
1101	44-45	0.2168851424699163
1101	46-47	0.18138851156084712
1101	48-49	0.28501272469463146
1101	50-51	0.5508440133408214
1101	52-53	0.6776392187134164
1101	54-55	0.5575393803720345
1101	56-57	0.6001175912944596
1101	58-59	0.3044219222901319
1101	60-61	0.5048800837390459
1101	62-63	0.6765918348116919
1101	64-65	0.7243014723935524
1101	66-67	0.7592611733078414
1101	68-69	0.8252839211758847
1101	70-71	0.3765806353950474
1101	72-73	0.2611514846236176
1101	74-75	0.2679563817886006
1101	76-77	0.4020079788131099
1101	78-79	0.4437074004027828
1101	80-81	0.4849067020892335
1101	82-83	0.6094848601685854
1101	84-85	0.477516372512909
1101	86-87	0.5334857720911792
1101	88-89	0.276473536483099
1101	90-91	0.5971420580858151
1101	92-93	0.4123348402175324
1101	94-95	0.2488000991329642
1101	96-97	0.17252030211864877
1101	98-99	0.3212187873888688
1101	100	0.36703048603276045
1102	1	0.8082316491852417
1102	2	0.7129233662034444
1102	3	0.7137084993894582
1102	4	0.045927568338271385
1102	5	0.6056368950721485
1102	6	0.8531250991005059
1102	7	0.4159033239123886
1102	8	-0.17439321922647721
1102	9	0.44130018069443366
1102	10-11	0.23461488116040385
1102	12-13	-0.12390120708831631
1102	14-15	0.1509720664706009
1102	16-17	0.23175611335471302
1102	18-19	0.38705758866755957
1102	20-21	0.3911276659289129
1102	22-23	0.34255685047234863
1102	24-25	0.6852115900669062
1102	26-27	0.5333029405187588
1102	28-29	0.35125815420138906
1102	30-31	0.3886571779524175
1102	32-33	0.529997253372116
1102	34-35	0.9522399645989417
1102	36-37	1.3880298165672649
1102	38-39	1.4839437373003719
1102	40-41	1.347430366149787
1102	42-43	1.3401880083896103
1102	44-45	1.2881917626789736
1102	46-47	1.5501515777629393
1102	48-49	1.5511451288758131
1102	50-51	1.527237741563816
1102	52-53	1.4799562918841467
1102	54-55	1.0529400772361477
1102	56-57	1.0548388456498579
1102	58-59	0.5964950930218436
1102	60-61	0.5695839164916592
1102	62-63	0.691016921919708
1102	64-65	0.6174896257036551
1102	66-67	0.5891392220883276
1102	68-69	0.9274790431271036
1102	70-71	0.8413889977643869
1102	72-73	0.84803301772466
1102	74-75	0.732660214541216
1102	76-77	1.1380010101719975
1102	78-79	0.9702753446536541
1102	80-81	1.0420321376293025
1102	82-83	0.9386312016319991
1102	84-85	0.8107567906313768
1102	86-87	0.6996878626835148
1102	88-89	0.6519961845667233
1102	90-91	1.1034835215004506
1102	92-93	1.2194428541548135
1102	94-95	1.5452112489587506
1102	96-97	1.3168234380071482
1102	98-99	1.5691456166571562
1102	100	1.6672047020606335
1103	1	-0.5708892299356378
1103	2	-0.4505931173130371
1103	3	-0.1364013907204331
1103	4	-1.0395669371562342
1103	5	-0.5759015664663139
1103	6	-0.9837979778225687
1103	7	-0.4427779947689281
1103	8	-0.22823937307263265
1103	9	-0.47408443469018025
1103	10-11	-0.07582467927915104
1103	12-13	-0.22565944884655664
1103	14-15	-0.2556213401228007
1103	16-17	-0.36780432620572157
1103	18-19	-0.6492061475961819
1103	20-21	-0.21700420220295058
1103	22-23	-0.1324980945825942
1103	24-25	-0.038085113229797685
1103	26-27	-0.14757618036036746
1103	28-29	-0.022917669974440003
1103	30-31	0.3659099252051661
1103	32-33	0.6604368138116712
1103	34-35	0.5527894151483927
1103	36-37	0.22649135502880569
1103	38-39	0.10130637466300385
1103	40-41	0.029957838677262316
1103	42-43	-0.16464715644555028
1103	44-45	-0.07851153402431876
1103	46-47	-0.02435391674255527
1103	48-49	0.1655407332714205
1103	50-51	-0.011773247447173674
1103	52-53	0.22149475342260772
1103	54-55	0.03381919811526757
1103	56-57	0.2626410434520565
1103	58-59	0.20253904906579834
1103	60-61	-0.0074490505413074
1103	62-63	-0.13337868247589668
1103	64-65	0.14540171361574394
1103	66-67	-0.0828387998896929
1103	68-69	-0.2257077700597101
1103	70-71	-0.3311384747630832
1103	72-73	0.03396708365872669
1103	74-75	-0.3020650601840593
1103	76-77	-0.04760338543239584
1103	78-79	-0.07642795204964159
1103	80-81	-0.33280302720586263
1103	82-83	-0.1533468203460231
1103	84-85	-0.30484760497301977
1103	86-87	-0.2959165329208808
1103	88-89	-0.28701480444426863
1103	90-91	-0.49827472025778974
1103	92-93	-0.648908794196835
1103	94-95	-0.8999535862060881
1103	96-97	-0.8614183202346055
1103	98-99	-0.8104148229032795
1103	100	-0.7251029902470592
1104	1	-1.1039150276673197
1104	2	-0.8815151364703517
1104	3	-1.3012953203202464
1104	4	-1.26172712990466
1104	5	-1.7514906832319
1104	6	-1.267455496774204
1104	7	-0.7218812444604161
1104	8	-1.08852614512411
1104	9	-1.0729855335912788
1104	10-11	-1.1828793892750369
1104	12-13	-0.73523810548404
1104	14-15	-1.1229011191672882
1104	16-17	-1.131834413765226
1104	18-19	-0.7181372165272535
1104	20-21	-0.7621649238342627
1104	22-23	-1.3236158004061807
1104	24-25	-1.3546509003838167
1104	26-27	-1.4972852947753594
1104	28-29	-1.4537074684112952
1104	30-31	-1.5679356409933405
1104	32-33	-1.1404534723727302
1104	34-35	-0.7757203104201587
1104	36-37	-0.7027264859537432
1104	38-39	-0.8813580197660968
1104	40-41	-0.8491777316347751
1104	42-43	-1.2373979350786826
1104	44-45	-0.9240924237228576
1104	46-47	-0.738022600999475
1104	48-49	-0.6922926098560396
1104	50-51	-0.5065284922024169
1104	52-53	-0.8229314086506108
1104	54-55	-0.8979842925117509
1104	56-57	-0.5614025600033123
1104	58-59	-0.698692072753559
1104	60-61	-0.3721578711324831
1104	62-63	-0.5083566456891546
1104	64-65	-0.49904207788687316
1104	66-67	-0.25471104529135147
1104	68-69	-0.3430022402953554
1104	70-71	-0.3570831183547867
1104	72-73	-0.7363214513356908
1104	74-75	-0.542236653296829
1104	76-77	-0.5788515490335016
1104	78-79	-0.4010004383868342
1104	80-81	-0.6864934544257011
1104	82-83	-0.37602120478511125
1104	84-85	-0.28777644084303233
1104	86-87	-0.2983258882714104
1104	88-89	-0.2894094686036368
1104	90-91	-0.2805806496225429
1104	92-93	-0.22859381505832488
1104	94-95	-0.062305939734912386
1104	96-97	-0.002336225858392993
1104	98-99	-0.2398383405620912
1104	100	-0.24784495416549035
1105	1	1.3789200281637761
1105	2	1.1313689620583673
1105	3	1.425063051573023
1105	4	1.7283257918763937
1105	5	1.555511062355638
1105	6	1.4084470828162772
1105	7	1.4773096895674591
1105	8	1.8571389791451018
1105	9	1.4710559171859217
1105	10-11	1.6110027420042243
1105	12-13	1.619725735916866
1105	14-15	1.961482799261134
1105	16-17	1.9403719534731465
1105	18-19	2.046347003915514
1105	20-21	1.9934666740710298
1105	22-23	1.790166028858735
1105	24-25	1.939452892805619
1105	26-27	1.6106086400006276
1105	28-29	1.5945594125285538
1105	30-31	1.649930308966482
1105	32-33	1.8651711985978707
1105	34-35	1.571707026034911
1105	36-37	1.5307389209344073
1105	38-39	1.7237512872633616
1105	40-41	1.924380773255642
1105	42-43	1.9761317537634184
1105	44-45	1.5988357301105118
1105	46-47	1.8295150122559072
1105	48-49	1.9810192961593067
1105	50-51	1.8667640183957914
1105	52-53	2.1148785716768934
1105	54-55	1.8729696849341515
1105	56-57	1.7149128648948597
1105	58-59	1.7814691862860883
1105	60-61	1.9690213702296298
1105	62-63	1.8714610373897322
1105	64-65	1.7887997663402224
1105	66-67	1.852351657321492
1105	68-69	1.8724531363913535
1105	70-71	1.7757339274461046
1105	72-73	1.4495282064737367
1105	74-75	1.3797660620615666
1105	76-77	1.6272386119484565
1105	78-79	1.5420740123072427
1105	80-81	1.6516102279327818
1105	82-83	1.740392859663089
1105	84-85	1.8165154878926657
1105	86-87	1.7181186546894374
1105	88-89	1.64518641402638
1105	90-91	1.520685594039314
1105	92-93	1.8273481095212105
1105	94-95	1.2917989617640764
1105	96-97	1.4164385379331321
1105	98-99	1.7042751503359135
1105	100	1.4746066265610054
1106	1	-0.3374266141200799
1106	2	0.2093939544387382
1106	3	-0.5789245538318326
1106	4	-0.7973777537905811
1106	5	-0.6311698276169295
1106	6	-0.6435695787706379
1106	7	-0.5841807097010552
1106	8	-0.39512151054300304
1106	9	-0.788671808101082
1106	10-11	-0.5752590684194203
1106	12-13	-0.4446855208138061
1106	14-15	-0.7100223312884992
1106	16-17	-0.7013531303427669
1106	18-19	-0.6737967530691407
1106	20-21	-0.7770936225864986
1106	22-23	-0.30579048846322365
1106	24-25	-0.25215535671181044
1106	26-27	-0.19826568693222413
1106	28-29	0.20672033907533205
1106	30-31	-0.2045220937562604
1106	32-33	-0.10168341889679056
1106	34-35	-0.3599449093506415
1106	36-37	-0.35223628987531086
1106	38-39	-0.2871066828677016
1106	40-41	-0.5962811184440469
1106	42-43	-0.6286635322266321
1106	44-45	-0.5419622989456734
1106	46-47	-0.4111509432454632
1106	48-49	-0.5414459075387512
1106	50-51	-0.37150175423450094
1106	52-53	-0.7302397865472265
1106	54-55	-0.6185725278058669
1106	56-57	-0.3644328630336169
1106	58-59	-0.19223039717423518
1106	60-61	0.053421451505666795
1106	62-63	-0.12422117331838578
1106	64-65	-0.0661098140722558
1106	66-67	-0.13193920928421932
1106	68-69	0.14385159214671006
1106	70-71	0.0769632274562646
1106	72-73	0.13890136506359596
1106	74-75	0.2429683377905114
1106	76-77	0.2709701978470669
1106	78-79	0.39779635305701433
1106	80-81	0.30682205359568826
1106	82-83	-0.048035465034669045
1106	84-85	-0.04334685082520551
1106	86-87	0.027208871086873643
1106	88-89	-0.04520269498510032
1106	90-91	0.0866207764024125
1106	92-93	0.1517537785245615
1106	94-95	0.32561741142373535
1106	96-97	0.387369656494549
1106	98-99	0.39630247940225516
1106	100	0.02532795136035304
1107	1	-0.4795834768651801
1107	2	0.20449199365442539
1107	3	-0.025002985204380934
1107	4	-0.25326010673175503
1107	5	-0.19979727859731966
1107	6	0.062312774170536045
1107	7	0.37660360402443516
1107	8	0.3646824110256226
1107	9	0.40250466248715355
1107	10-11	0.2801330884433284
1107	12-13	0.08227526349992331
1107	14-15	0.22870315890757809
1107	16-17	-0.05184332642119216
1107	18-19	0.2967914822249753
1107	20-21	-0.1030740147433491
1107	22-23	-0.36706499826713923
1107	24-25	-0.34284163122161004
1107	26-27	-0.36738333399105016
1107	28-29	0.04250465280082949
1107	30-31	0.0528308474202035
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2216	92-93	-1.090893280298971
2216	94-95	-1.2160492552429325
2216	96-97	-1.292252192244952
2216	98-99	-1.368753543006708
2216	100	-1.8227112643259211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8296.0
3	1068.0
4	837.0
5	853.0
6	2107.0
7	4241.0
8	4441.0
9	2356.0
10	1348.0
11	1350.0
12	1203.0
13	728.0
14	620.0
15	572.0
16	542.0
17	538.0
18	497.0
19	409.0
20	369.0
21	424.0
22	481.0
23	853.0
24	1454.0
25	1150.0
26	1068.0
27	957.0
28	1155.0
29	1295.0
30	1592.0
31	1989.0
32	2513.0
33	3489.0
34	4968.0
35	8333.0
36	14463.0
37	25661.0
38	24316.0
39	6545.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.2257001281377	20.282051092133234	12.8848760471369	18.607372732592157
2	16.449931128456537	28.22918672334375	31.504658086111647	23.816224062088068
3	21.95517568288203	32.848699413402855	25.13553949161581	20.060585412099307
4	12.25675025000926	23.972739731101154	31.839697766583946	31.93081225230564
5	11.9573591726672	45.838148576169736	27.86173585800219	14.342756393160874
6	26.017826315672487	41.24428572487015	15.054568086004934	17.683319873452422
7	23.097669042573685	43.257561163552296	16.772372301172148	16.872397492701865
8	22.641383603058866	38.94347619894481	16.770407255913213	21.64473294208311
9	21.48652553738543	28.390844626886686	16.91773057002497	33.20489926570292
10-11	21.391600748411477	35.541394194253535	20.963672403252996	22.103332654081992
12-13	23.57888652582125	35.55655183145976	21.057896803930433	19.806664838788564
14-15	21.669797444248076	36.79756651512962	21.21703884016732	20.31559720045498
16-17	19.30073452877923	37.75063062796099	22.426648985624276	20.521985857635507
18-19	19.79890635882693	37.85102473288778	22.394451771609784	19.955617136675507
20-21	23.19125132437819	34.12524357445043	21.97023020100912	20.71327490016226
22-23	22.3654974225277	34.412217811222376	21.9336967470522	21.288588019197725
24-25	22.93943432548497	34.63682807641049	20.94180364282541	21.481933955279136
26-27	21.846274783176177	32.79221621942312	23.939426524626516	21.422082472774196
28-29	20.739936887953895	36.432761966843955	22.67700262226107	20.150298522941082
30-31	21.551574057954372	35.77347196550741	22.00199280663479	20.672961169903434
32-33	20.580946983587246	35.53762783685776	23.097124527267543	20.784300652287452
34-35	21.219491428169505	35.45602286315057	22.463341588648387	20.86114412003154
36-37	20.502081203987615	35.65487564621013	22.90916766653335	20.933875483268896
38-39	20.216607835366194	36.30329541708491	22.82530992921724	20.65478681833166
40-41	19.991109794043563	36.365017039561415	23.201955845310415	20.441917321084606
42-43	19.27788045914002	36.050047595589355	22.694391869117688	21.977680076152943
44-45	19.794730097116148	35.46628343691895	23.1111983584334	21.6277881075315
46-47	20.255804305631695	35.61776776850933	23.1561050775636	20.97032284829538
48-49	19.8793975805436	34.49873693412056	22.6729585373623	22.948906947973537
50-51	18.41482430765698	33.93101200419276	24.03986829093037	23.614295397219887
52-53	19.55277671801672	33.58952799682936	22.283750097230502	24.573945187923414
54-55	18.805185185185184	32.343333333333334	22.8262962962963	26.025185185185183
56-57	20.843333333333334	31.124074074074077	22.298148148148147	25.734444444444442
58-59	21.327753189755747	30.344252884205847	22.502175885631747	25.825818040406663
60-61	20.35665345727936	30.505166475315733	23.379134106144217	25.759045961260696
62-63	19.912297595591177	29.98918534540229	23.00373327802551	27.094783780981025
64-65	19.413714560841466	30.272031999407417	22.734393807522082	27.57985963222903
66-67	20.310590291923763	28.716824319819857	22.315760623995377	28.656824764261007
68-69	20.343326975426383	28.610210922019963	23.69993148275032	27.34653061980334
70-71	20.935408727469344	28.095962906588746	24.140344639861343	26.828283726080564
72-73	20.759251784067636	27.017098163530584	26.48641442215466	25.737235630247124
74-75	20.54240024886861	28.67951500248128	25.062402322773703	25.715682425876413
76-77	20.92987946006999	26.688021923084044	25.622604477197402	26.759494139648567
78-79	22.222798272835675	26.530710492597336	26.778823721105923	24.46766751346107
80-81	22.638756716124995	25.833614136170247	26.732306644103698	24.795322503601064
82-83	22.308464270240442	25.447617213682367	27.505823189811917	24.73809532626527
84-85	22.305245657623367	26.116465670138595	27.004010056614742	24.5742786156233
86-87	22.81134401972873	26.664148735665382	25.741401080488625	24.78310616411726
88-89	22.52486429243222	26.367628653736496	25.242348166744428	25.86515888708686
90-91	23.473086706828944	25.23299428668758	25.45960136408574	25.834317642397743
92-93	23.95306471559647	26.784881180713434	24.86466672097277	24.397387382717326
94-95	22.45796444612303	27.172315223146676	25.392769995815883	24.97695033491441
96-97	20.722495408495764	28.273298181171867	26.106774690443746	24.89743171988862
98-99	20.914672077729723	30.244682741868594	24.07207180520173	24.768573375199953
100	20.47191610380377	30.12057115772011	23.6854188884939	25.722093849982226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1617.0
1	988.5
2	331.0
3	258.5
4	182.5
5	141.5
6	122.5
7	141.0
8	169.0
9	161.5
10	140.0
11	116.0
12	112.0
13	105.0
14	92.0
15	104.5
16	121.5
17	121.5
18	121.0
19	149.5
20	187.5
21	246.5
22	329.0
23	428.0
24	564.0
25	698.0
26	899.0
27	1164.0
28	1483.5
29	1723.5
30	1868.5
31	2119.5
32	2308.0
33	2537.0
34	2796.5
35	2922.5
36	2950.0
37	2973.5
38	3122.0
39	3421.5
40	3532.0
41	3414.5
42	3252.0
43	3239.5
44	3264.5
45	3384.5
46	4065.5
47	5336.0
48	7862.5
49	8186.5
50	6039.0
51	4922.5
52	4168.5
53	3682.5
54	3429.0
55	3222.0
56	3072.0
57	2743.5
58	2271.5
59	1996.0
60	1936.5
61	1780.5
62	1875.5
63	1956.5
64	1712.0
65	1447.5
66	1010.5
67	588.0
68	441.0
69	337.0
70	237.0
71	284.5
72	285.5
73	213.0
74	186.0
75	146.5
76	101.5
77	82.0
78	55.0
79	31.5
80	20.5
81	15.5
82	7.5
83	4.0
84	4.0
85	3.5
86	3.0
87	1.5
88	0.5
89	0.0
90	1.0
91	1.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05404044890586598
2	0.03701400609990821
3	0.05033904829587516
4	0.0658849308578366
5	0.07106689171182375
6	0.08513221402978888
7	0.08735305439578336
8	0.09771697610375767
9	0.09253501524977051
10-11	0.09734683604275858
12-13	0.1014183767137485
14-15	0.09882739628675491
16-17	0.07365787213881733
18-19	0.09031417488377602
20-21	0.08513221402978888
22-23	0.05033904829587516
24-25	0.017766722927955936
26-27	0.1772970892185603
28-29	0.06366409049184212
30-31	0.07291759201681916
32-33	0.072177311894821
34-35	0.0144354623789642
36-37	0.04885848805187883
38-39	0.06921619140682835
40-41	0.07698913268780906
42-43	0.06847591128483019
44-45	0.06736549110183293
46-47	0.07254745195582009
48-49	0.07032661158982559
50-51	0.06625507091883569
52-53	0.07069675165082467
54-55	0.06218353024784578
56-57	0.06218353024784578
58-59	0.06033282994285038
60-61	0.058482129637854964
62-63	0.06070297000384946
64-65	0.06033282994285038
66-67	0.061443250125847615
68-69	0.06033282994285038
70-71	0.05441058896686506
72-73	0.05144946847887241
74-75	0.05404044890586598
76-77	0.04922862811287791
78-79	0.04811820792988066
80-81	0.040345266648899945
82-83	0.04700778774688342
84-85	0.03590358591691096
86-87	0.038864706404903616
88-89	0.037754286221906366
90-91	0.03590358591691096
92-93	0.03479316573391371
94-95	0.03664386603890912
96-97	0.03849456634390453
98-99	0.03849456634390453
100	0.044416807319889846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	135084.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46933759734683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97641398732691	41.877646501436146
2	7.251818821872799	7.319889846317847
3	2.703297348040366	4.093008794527849
4	1.5724008448720956	3.174321163128128
5	1.0370218258624737	2.6168902312635103
6	0.7832668387702418	2.371857510882118
7	0.5427129781741374	1.9173255159752451
8	0.40630133771415156	1.6404607503479316
9	0.3608307908941563	1.6389801901039354
>10	2.22805679417977	20.264428059577742
>50	0.09534146913869984	3.2601936572799146
>100	0.035203003989673784	4.807379112256078
>500	0.002933583665806149	1.0223268484794645
>1k	0.004400375498709223	3.995291818424092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2423	1.7936987356015517	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1821	1.3480501021586568	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1153	0.8535429806638832	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	812	0.6011074590625092	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	569	0.42121938941695536	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	476	0.3523733380711261	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	465	0.3442302567291463	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	454	0.3360871753871665	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	449	0.3323857747771757	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	436	0.32276213319119956	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	420	0.31091765123922893	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	402	0.29759260904326196	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	359	0.2657605637973409	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	301	0.2228243167214474	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	299	0.22134375647745105	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	286	0.21172011489147494	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	263	0.19469367208551716	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	258	0.19099227147552633	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	254	0.18803115098753367	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	201	0.14879630452163098	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	182	0.13473098220366586	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	169	0.12510734061768974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.402801219981641E-4	0.0	0.0	0.0	0.0
3	7.402801219981641E-4	0.0	0.0	0.0	0.0
4	7.402801219981641E-4	0.0	0.0	0.0	0.0
5	7.402801219981641E-4	0.0	0.0	0.0	0.0
6	7.402801219981641E-4	0.0	0.0	0.0	0.0
7	7.402801219981641E-4	0.0	0.0	0.0	0.0
8	7.402801219981641E-4	0.0	0.0	0.0	0.0
9	7.402801219981641E-4	0.0	0.0	0.0	0.0
10-11	7.402801219981641E-4	0.0	0.0	0.0	0.0
12-13	7.402801219981641E-4	0.0	0.0	0.0	0.0
14-15	7.402801219981641E-4	0.0	0.0	0.0	0.0
16-17	7.402801219981641E-4	0.0	0.0	7.402801219981641E-4	0.0
18-19	7.402801219981641E-4	0.0	0.0	0.0033312605489917383	0.0
20-21	7.402801219981641E-4	0.0	0.0	0.004441680731988985	0.0
22-23	7.402801219981641E-4	0.0	0.0	0.01073406176897338	0.0
24-25	7.402801219981641E-4	0.0	0.0	0.025539664208936663	0.0
26-27	7.402801219981641E-4	0.0	0.0	0.05478072902786414	0.0
28-29	7.402801219981641E-4	0.0	0.0	0.12917888128867963	0.0
30-31	7.402801219981641E-4	0.0	0.0	0.2783453258713097	0.0
32-33	7.402801219981641E-4	0.0	0.0	0.49117586094578186	0.0
34-35	7.402801219981641E-4	0.0	0.0	0.8446596191999052	0.0
36-37	7.402801219981641E-4	0.0	0.0	1.3313937994136982	0.0
38-39	7.402801219981641E-4	0.0	0.0	1.899558793047289	0.0
40-41	7.402801219981641E-4	0.0	0.0	2.507698913268781	0.0
42-43	7.402801219981641E-4	0.0	0.0	2.9429836250037016	0.0
44-45	7.402801219981641E-4	0.0	0.0	3.347176571614699	0.0
46-47	7.402801219981641E-4	0.0	0.0	3.8242871102425156	0.0
48-49	7.402801219981641E-4	0.0	0.0	4.250318320452459	0.0
50-51	7.402801219981641E-4	0.0	0.0	4.650809866453466	0.0
52-53	7.402801219981641E-4	0.0	0.0	5.02428118800154	0.0
54-55	7.402801219981641E-4	0.0	0.0	5.437727636137515	0.0
56-57	7.402801219981641E-4	0.0	0.0	5.917799295253324	0.0
58-59	7.402801219981641E-4	0.0	0.0	6.3086671996683545	0.0
60-61	7.402801219981641E-4	0.0	0.0	6.7454324716472716	0.0
62-63	7.402801219981641E-4	0.0	0.0	7.139631636611293	0.0
64-65	7.402801219981641E-4	0.0	0.0	7.547155843771283	0.0
66-67	7.402801219981641E-4	0.0	0.0	8.042403245388055	0.0
68-69	7.402801219981641E-4	0.0	0.0	8.62500370140061	0.0
70-71	7.402801219981641E-4	0.0	0.0	9.240176482781084	0.0
72-73	7.402801219981641E-4	0.0	0.0	9.811302596902667	0.0
74-75	7.402801219981641E-4	0.0	0.0	10.426105238222142	0.0
76-77	7.402801219981641E-4	0.0	0.0	11.066447543750556	0.0
78-79	7.402801219981641E-4	0.0	0.0	11.808578366053714	0.0
80-81	7.402801219981641E-4	0.0	0.0	12.548858488051877	0.0
82-83	7.402801219981641E-4	0.0	0.0	13.256196144621125	0.0
84-85	7.402801219981641E-4	0.0	0.0	13.982410944301325	0.0
86-87	7.402801219981641E-4	0.0	0.0	14.677904118918597	0.0
88	7.402801219981641E-4	0.0	0.0	15.267537236090137	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCTAT	15	6.843654E-4	94.15591	1
GTTTAAG	25	0.0051918766	56.49354	1
GTAACAA	25	0.0051918766	56.49354	1
ATAGAAC	25	0.0052071563	56.451633	3
TACATGA	60	2.5152985E-8	54.883533	2
TACATAA	45	1.5976202E-5	52.27003	2
TAACAAC	125	0.0	45.19483	1
GGAAACA	190	0.0	44.600166	1
GAGAACA	220	0.0	42.798138	1
GTACATA	80	2.405559E-7	41.193207	1
ACGTGGG	50	0.0016890947	37.634422	3
GAGTGTT	90	6.128139E-7	36.561897	7
GTACAAA	160	1.8189894E-12	35.308464	1
GCAGAAA	180	0.0	33.97552	3
TTGAGTG	85	1.728091E-5	33.219166	5
CATGAAA	85	1.7324883E-5	33.20684	4
AGAACAA	185	0.0	33.057262	2
AGCACCA	60	0.0041220514	31.373657	5
ACATAAA	105	2.032979E-6	31.362019	3
GACCAAT	60	0.0041370555	31.35039	9
>>END_MODULE