##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033053_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135084
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47533386633502	33.0	31.0	34.0	31.0	34.0
2	32.40126143732788	34.0	31.0	34.0	31.0	34.0
3	32.416785111486185	34.0	31.0	34.0	30.0	34.0
4	36.058311865209795	37.0	35.0	37.0	35.0	37.0
5	36.08730123478724	37.0	35.0	37.0	35.0	37.0
6	36.22124752006159	37.0	37.0	37.0	35.0	37.0
7	36.170338456071775	37.0	36.0	37.0	35.0	37.0
8	36.17636433626484	37.0	37.0	37.0	35.0	37.0
9	37.91144769180658	39.0	38.0	39.0	35.0	39.0
10-11	37.87780936306298	39.0	38.0	39.0	35.0	39.0
12-13	37.84341964999556	39.0	38.0	39.0	35.0	39.0
14-15	39.0512718012496	40.5	38.0	41.0	36.0	41.0
16-17	39.08466583755293	40.0	38.5	41.0	36.0	41.0
18-19	39.2776753723609	41.0	39.0	41.0	36.0	41.0
20-21	39.31091765123922	41.0	39.0	41.0	36.0	41.0
22-23	39.14668280477332	41.0	39.0	41.0	35.0	41.0
24-25	38.97912780196026	41.0	38.5	41.0	35.0	41.0
26-27	38.69510452755323	40.0	38.0	41.0	35.0	41.0
28-29	38.52644280595777	40.0	38.0	41.0	35.0	41.0
30-31	38.110794024458855	40.0	37.0	41.0	34.5	41.0
32-33	37.57950978650321	40.0	35.0	41.0	33.5	41.0
34-35	37.16315033608717	40.0	35.0	41.0	33.0	41.0
36-37	36.76557179236623	40.0	35.0	41.0	33.0	41.0
38-39	36.3785570459862	40.0	35.0	41.0	31.5	41.0
40-41	36.0329720766338	40.0	35.0	41.0	30.5	41.0
42-43	35.50378653282402	40.0	35.0	41.0	28.0	41.0
44-45	34.732207367267776	40.0	35.0	41.0	16.5	41.0
46-47	33.52016152912262	39.0	35.0	41.0	2.0	41.0
48-49	32.42836309259424	39.0	33.5	41.0	2.0	41.0
50-51	31.83551715969323	39.0	33.0	41.0	2.0	41.0
52-53	31.60455716443102	39.0	33.0	41.0	2.0	41.0
54-55	31.380544698113766	38.0	33.0	41.0	2.0	41.0
56-57	31.14629045630867	37.5	32.0	41.0	2.0	41.0
58-59	30.884024014687157	37.0	32.0	40.5	2.0	41.0
60-61	30.558585768854932	36.5	31.0	40.0	2.0	41.0
62-63	30.301697462319744	36.0	31.0	40.0	2.0	41.0
64-65	29.93881954931746	35.5	30.5	39.5	2.0	41.0
66-67	29.46805691273578	35.0	30.0	39.0	2.0	41.0
68-69	28.913124426282906	35.0	28.5	38.5	2.0	40.5
70-71	28.271553255751975	35.0	26.5	37.0	2.0	40.0
72-73	27.816776968404845	35.0	26.0	37.0	2.0	39.0
74-75	27.53976044535252	35.0	25.0	36.0	2.0	39.0
76-77	27.255348523881437	35.0	24.5	36.0	2.0	38.5
78-79	26.962182789967724	35.0	23.5	35.0	2.0	37.0
80-81	26.721591750318318	35.0	22.5	35.0	2.0	37.0
82-83	26.489117882206628	35.0	20.5	35.0	2.0	36.5
84-85	26.386159722839125	35.0	20.0	35.0	2.0	36.0
86-87	26.315729472032217	35.0	20.0	35.0	2.0	36.0
88-89	26.160200319801014	35.0	19.5	35.0	2.0	35.5
90-91	26.009738385004887	34.5	18.0	35.0	2.0	35.0
92-93	25.87864958100145	34.0	15.0	35.0	2.0	35.0
94-95	25.727780492138223	34.0	4.5	35.0	2.0	35.0
96-97	25.591798436528386	34.0	2.0	35.0	2.0	35.0
98-99	25.48473912528501	34.0	2.0	35.0	2.0	35.0
100	25.37886796363744	34.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09031795640068907
1101	2	0.14322162927916082
1101	3	0.18071294915485225
1101	4	-0.018929356876874692
1101	5	0.07050159761663366
1101	6	0.2040948566516647
1101	7	0.17866975440377075
1101	8	0.20374079446508375
1101	9	0.29055796936142997
1101	10-11	0.3895165333633557
1101	12-13	0.33762325819696315
1101	14-15	0.4343541905518009
1101	16-17	0.30551084102123127
1101	18-19	0.2326940458265554
1101	20-21	0.33728674605200126
1101	22-23	0.44726673422634633
1101	24-25	0.6380552355806799
1101	26-27	0.626194941447956
1101	28-29	0.7082958124107108
1101	30-31	0.9621812909031746
1101	32-33	0.4061337177521622
1101	34-35	0.41146615473169135
1101	36-37	0.2183568605548345
1101	38-39	0.5115925549766231
1101	40-41	0.9696196175059555
1101	42-43	0.9249089720284474
1101	44-45	0.697766505299164
1101	46-47	0.42127313557134727
1101	48-49	-0.5838277491549704
1101	50-51	-0.3764266301048309
1101	52-53	0.010601529736135262
1101	54-55	-0.2514253332977887
1101	56-57	0.12227243425950363
1101	58-59	0.19531160566702965
1101	60-61	0.028110733267599386
1101	62-63	0.056620808510579934
1101	64-65	0.3556920335019029
1101	66-67	0.1825919081837455
1101	68-69	0.025696425045264704
1101	70-71	0.07744599959150733
1101	72-73	0.3091446127120854
1101	74-75	0.5617959659525589
1101	76-77	0.4084521303991231
1101	78-79	0.3700960639425297
1101	80-81	0.33613184426465637
1101	82-83	0.35074688716724367
1101	84-85	0.26208398117501375
1101	86-87	0.25600950198566963
1101	88-89	0.18877334346174734
1101	90-91	0.6366407868701067
1101	92-93	0.7939809993380784
1101	94-95	0.5024458409249348
1101	96-97	0.6080306650132137
1101	98-99	0.4311746566534431
1101	100	0.28004819074041976
1102	1	0.2756141236480758
1102	2	0.10719375471470016
1102	3	0.1820021477611249
1102	4	0.11096611350640018
1102	5	0.16910786939364186
1102	6	0.23402517024052116
1102	7	0.19605651398565271
1102	8	0.3281658815730992
1102	9	0.44742208085968826
1102	10-11	0.39606705601143943
1102	12-13	0.44173475645480664
1102	14-15	0.41320436476782874
1102	16-17	0.3563993427633889
1102	18-19	0.32563829669764033
1102	20-21	0.5054052129509614
1102	22-23	0.41141307568975805
1102	24-25	0.6806510543960087
1102	26-27	0.7561426766395911
1102	28-29	0.9641843141528739
1102	30-31	1.0360314651192084
1102	32-33	1.2205936480657513
1102	34-35	0.4411177226759406
1102	36-37	0.34309553651301883
1102	38-39	0.07615701490693993
1102	40-41	-0.31576365775536175
1102	42-43	-0.4023384147311404
1102	44-45	-0.5035923797182491
1102	46-47	-0.3911484672161123
1102	48-49	-0.322904404207236
1102	50-51	-0.43114788446023056
1102	52-53	-0.32054829604783563
1102	54-55	-0.3027667967124259
1102	56-57	-0.3742606667857906
1102	58-59	-0.28383473579638263
1102	60-61	-0.2809310785790906
1102	62-63	-0.2546161252873347
1102	64-65	-0.0898131929789372
1102	66-67	-0.5738889280531936
1102	68-69	-0.300209498299683
1102	70-71	-0.4503240352517004
1102	72-73	-0.38197036986630906
1102	74-75	-0.21463260547601237
1102	76-77	-0.21086842709216924
1102	78-79	-0.4191896503431849
1102	80-81	-0.3199134519025897
1102	82-83	-0.3511172243310092
1102	84-85	-0.394362011856348
1102	86-87	-0.23775356421642257
1102	88-89	-0.3091012209981834
1102	90-91	-0.27315015389644515
1102	92-93	-0.13467753724728482
1102	94-95	0.30805559702249496
1102	96-97	0.1020376336543265
1102	98-99	0.08756838487643748
1102	100	0.23976944509582054
1103	1	0.22352621156016994
1103	2	0.08653441405536455
1103	3	0.2681559939149665
1103	4	0.34679028933057765
1103	5	0.3185584188441908
1103	6	0.3096295658449151
1103	7	0.2749576128867517
1103	8	0.38552851893573603
1103	9	0.3061033995410014
1103	10-11	0.18079233073671475
1103	12-13	0.44898750370755636
1103	14-15	0.42155601311947777
1103	16-17	0.6048608812249228
1103	18-19	0.6866273076866563
1103	20-21	0.6592513667971076
1103	22-23	0.8286658229425043
1103	24-25	0.7954862192311793
1103	26-27	0.8791097096066238
1103	28-29	0.7452832152517743
1103	30-31	1.0111963002840412
1103	32-33	0.8387255161976199
1103	34-35	0.9446342061924256
1103	36-37	1.0749636683811516
1103	38-39	1.0152778940278182
1103	40-41	1.1489616169699133
1103	42-43	0.7439253215326005
1103	44-45	0.5290449829191104
1103	46-47	0.7651152690476195
1103	48-49	0.7822604309575993
1103	50-51	0.515994972682627
1103	52-53	0.62967148417194
1103	54-55	0.48822221427658263
1103	56-57	0.34650856398343777
1103	58-59	0.40869273673109063
1103	60-61	0.4499480423000328
1103	62-63	0.5843948637236558
1103	64-65	0.5967802136144726
1103	66-67	0.5495176653534024
1103	68-69	0.27814215005196274
1103	70-71	0.13165398672632378
1103	72-73	-0.1561461940421367
1103	74-75	-0.3599073307507368
1103	76-77	-0.23383546005920053
1103	78-79	-0.09831052946406516
1103	80-81	-0.16189147388061187
1103	82-83	0.19646519325140588
1103	84-85	0.019923702429370138
1103	86-87	-0.025885432348289328
1103	88-89	-0.21547484737180866
1103	90-91	-0.19644685719314836
1103	92-93	-0.18193028450003013
1103	94-95	-0.21425209528519673
1103	96-97	-0.008292036675342729
1103	98-99	-0.06276128545323445
1103	100	0.2736155989419764
1104	1	0.19329174014922756
1104	2	0.09411506869484043
1104	3	0.0942710553241497
1104	4	0.05293708371266348
1104	5	0.2435158143898164
1104	6	0.1936279204315028
1104	7	0.06490296165562626
1104	8	0.12440728722626915
1104	9	0.2406688341064367
1104	10-11	0.2931946343154834
1104	12-13	0.22121527593532875
1104	14-15	0.060446534362938564
1104	16-17	0.3423733687374124
1104	18-19	0.3637589995242294
1104	20-21	0.1589575429738801
1104	22-23	0.23783018798922484
1104	24-25	0.2221331017604058
1104	26-27	0.041138856929883616
1104	28-29	0.22309951659749316
1104	30-31	0.190546361987046
1104	32-33	0.3340390262170061
1104	34-35	0.2548533987645598
1104	36-37	-0.021763134227995806
1104	38-39	-0.06552365736197174
1104	40-41	-0.18821591138409133
1104	42-43	0.06362033240407072
1104	44-45	0.28309975167976376
1104	46-47	0.39745351903293624
1104	48-49	0.25171744453225386
1104	50-51	-0.19351610447550627
1104	52-53	-0.001006661212084481
1104	54-55	-0.24201813361082003
1104	56-57	-0.27711780964293453
1104	58-59	-0.1199844684167033
1104	60-61	-0.1050410862185096
1104	62-63	-0.4082448495042925
1104	64-65	-0.5046183877841308
1104	66-67	-0.5074107003984949
1104	68-69	-0.19125609875804628
1104	70-71	-0.05953717505307665
1104	72-73	-0.3382728908747126
1104	74-75	-0.2958243549030577
1104	76-77	-0.4925032628446502
1104	78-79	-0.638593775629662
1104	80-81	-0.304160969091285
1104	82-83	-0.43826772089327193
1104	84-85	-0.18137499886933384
1104	86-87	-0.259739813261497
1104	88-89	-0.2988032836414263
1104	90-91	-0.23837551676886548
1104	92-93	-0.4293299436643956
1104	94-95	-0.20509948319140747
1104	96-97	-0.39907772157867427
1104	98-99	-0.12085025530690885
1104	100	-0.25914575734879364
1105	1	5.290015163268436E-4
1105	2	0.2436260271055275
1105	3	0.2073463372503923
1105	4	-0.058863642230086555
1105	5	0.08294946820932836
1105	6	0.05096077349736561
1105	7	0.11425044440755983
1105	8	0.07348046336436198
1105	9	0.06403199943851234
1105	10-11	0.01987164520463125
1105	12-13	0.10958079642519891
1105	14-15	0.29689052316901154
1105	16-17	0.15466729243030386
1105	18-19	0.1336323751580366
1105	20-21	-0.04344748875148241
1105	22-23	0.12415918967939632
1105	24-25	0.042753200954109616
1105	26-27	-0.4345753100369478
1105	28-29	-0.5947498087190723
1105	30-31	-0.6681580241480063
1105	32-33	-0.42929532306674645
1105	34-35	-0.18581047865640699
1105	36-37	0.06929834924433464
1105	38-39	0.2186884730860683
1105	40-41	-0.20811006782197694
1105	42-43	-0.16942945840249024
1105	44-45	0.24986431902342332
1105	46-47	0.6953948340422116
1105	48-49	1.01352786818358
1105	50-51	1.0994516714243048
1105	52-53	0.8511171369647421
1105	54-55	0.7155751721994932
1105	56-57	0.779877009009919
1105	58-59	0.5682896165352211
1105	60-61	0.5861007496962607
1105	62-63	0.6736592263040819
1105	64-65	0.9277885834829434
1105	66-67	1.0602931592895182
1105	68-69	1.0174662974072
1105	70-71	1.7018299247779076
1105	72-73	1.6608127537458302
1105	74-75	1.9290239452271685
1105	76-77	1.7774809437442478
1105	78-79	1.590950986222321
1105	80-81	1.4711154156029629
1105	82-83	1.5274023907689163
1105	84-85	1.7519740355159712
1105	86-87	1.739359685228436
1105	88-89	2.0037929314814633
1105	90-91	2.062364279856329
1105	92-93	1.915063395395201
1105	94-95	1.5026669959862282
1105	96-97	1.6872041769555857
1105	98-99	1.622742330102195
1105	100	1.7193253296258
1106	1	-0.046458705483573226
1106	2	0.11907050541498165
1106	3	0.15799654552022702
1106	4	0.08903334039715816
1106	5	0.03451403185862745
1106	6	0.05548175287356827
1106	7	0.039501892136911465
1106	8	0.12351602162912201
1106	9	0.019634966013747146
1106	10-11	0.24640319046521597
1106	12-13	0.12295324384976425
1106	14-15	0.37439484095830267
1106	16-17	0.48341614948607514
1106	18-19	0.3412545431962428
1106	20-21	0.49875255188653256
1106	22-23	0.13778562470936606
1106	24-25	0.23149139053046497
1106	26-27	0.40076452537908125
1106	28-29	0.3665932777383105
1106	30-31	0.39977096091752173
1106	32-33	0.18452922229543844
1106	34-35	0.31545945937062214
1106	36-37	0.5838518390340255
1106	38-39	0.4785939896968543
1106	40-41	-0.07079166895984201
1106	42-43	-0.11418715422693992
1106	44-45	-0.07273803798155853
1106	46-47	0.5915266028119035
1106	48-49	0.40367822884598326
1106	50-51	0.03420225559579393
1106	52-53	-0.2772989963279535
1106	54-55	-0.1085511104379151
1106	56-57	-0.0026080057213668795
1106	58-59	0.05626330341929986
1106	60-61	0.2149232631576048
1106	62-63	0.14300292233171774
1106	64-65	0.18143330562050153
1106	66-67	0.12627913917370037
1106	68-69	0.19911823279275254
1106	70-71	0.2448300263729486
1106	72-73	0.3823153444194034
1106	74-75	0.36326655418785236
1106	76-77	0.4664985196865423
1106	78-79	0.34068429923664567
1106	80-81	0.31526862092654184
1106	82-83	0.4402973414953202
1106	84-85	0.48596011699519437
1106	86-87	0.22599993718413813
1106	88-89	0.5033357537917311
1106	90-91	0.5040487256553767
1106	92-93	-0.005755968619833851
1106	94-95	0.31629089114014164
1106	96-97	0.1728919753910212
1106	98-99	0.04314261456831403
1106	100	0.06304675602019216
1107	1	0.06138443177133013
1107	2	-0.2240667494869797
1107	3	0.08446713375552406
1107	4	-0.06292744391657124
1107	5	-0.0733291053962759
1107	6	0.050579792089259
1107	7	0.0836195391957375
1107	8	0.06469249221735396
1107	9	-0.05389444575095581
1107	10-11	0.05277573948482939
1107	12-13	-0.02165459928748703
1107	14-15	0.1268458213504644
1107	16-17	0.06920046321156548
1107	18-19	0.09860748437271383
1107	20-21	0.15561529698457122
1107	22-23	0.10102091882701103
1107	24-25	0.08198158660889021
1107	26-27	-0.07962763148366037
1107	28-29	0.12394621891477442
1107	30-31	0.0223199805253671
1107	32-33	-0.016451169861419146
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2216	68-69	-1.0781926915769944
2216	70-71	-1.6298198335710303
2216	72-73	-1.6554997816310149
2216	74-75	-1.867825882786935
2216	76-77	-2.152549099361078
2216	78-79	-1.9733913310154527
2216	80-81	-2.1063000065244353
2216	82-83	-2.5068315100452985
2216	84-85	-2.4464628521924787
2216	86-87	-2.3552325558130605
2216	88-89	-2.0975045823427223
2216	90-91	-2.186427464820813
2216	92-93	-2.0481229153985474
2216	94-95	-2.053457008019521
2216	96-97	-2.077458164665
2216	98-99	-2.4512551445353274
2216	100	-2.5735078658285495
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	16.0
6	47.0
7	119.0
8	145.0
9	248.0
10	367.0
11	685.0
12	1076.0
13	1622.0
14	1723.0
15	2348.0
16	3249.0
17	4780.0
18	5576.0
19	2130.0
20	800.0
21	610.0
22	737.0
23	801.0
24	967.0
25	1346.0
26	1675.0
27	1954.0
28	1184.0
29	1442.0
30	1591.0
31	1993.0
32	2390.0
33	3010.0
34	4305.0
35	7447.0
36	12740.0
37	27479.0
38	30083.0
39	8390.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.74764860101018	17.610682387095817	14.421666938219305	19.2200020736747
2	17.079252003561887	22.212822796081923	37.14603739982191	23.561887800534283
3	21.30425448434643	27.491653156254397	32.350959794493676	18.8531325649055
4	11.933668936926265	19.816405093278057	35.75732899022802	32.492596979567665
5	10.650488958640244	38.71841756549677	34.13456911676525	16.496524359097737
6	26.356193183500636	38.20363625595925	15.494803233543571	19.945367326996536
7	21.820496876017888	37.68544017056054	20.655295963992774	19.838766989428798
8	25.9558937838218	29.416728974038186	20.40005033942095	24.227326902719067
9	25.105119777323736	15.52737555891149	21.376328802818986	37.99117586094578
10-11	24.418970488623703	25.64302820107563	25.440930979727355	24.49707033057331
12-13	29.248467620147462	24.489576855882266	27.14163039294069	19.12032513102958
14-15	24.65799058363685	24.33855971099464	30.793432234757635	20.21001747061088
16-17	18.402253412691362	27.0553877587279	31.50706227236386	23.03529655621687
18-19	18.10768894794701	23.35259302579516	37.996498462062355	20.54321956419548
20-21	20.510941340203132	20.014213378342365	38.89949957063753	20.575345710816972
22-23	19.548207041544522	21.672070711557254	39.263717390660624	19.5160048562376
24-25	20.78959446854503	21.89929080114301	38.856398335825645	18.45471639448631
26-27	19.981344871875546	20.06314612813556	41.525056724174306	18.430452275814588
28-29	19.16185174355121	22.205154589568675	40.2458479384973	18.387145728382816
30-31	19.769920938082972	21.840484439311837	40.138728494862455	18.25086612774274
32-33	19.756225755826005	21.498475022948686	40.3608125314619	18.384486689763406
34-35	20.808533949246392	20.217050131769863	39.98623078973084	18.988185129252912
36-37	19.212119866157355	21.319697373486125	40.06321992241864	19.404962837937877
38-39	19.195463563412396	21.19718101329543	40.06210950223564	19.545245921056527
40-41	19.389488723641303	21.10842552939478	39.53961808807146	19.962467658892464
42-43	19.98312161321844	20.727843415948595	37.96711675698084	21.321918213852122
44-45	20.01569393858636	21.18163513073347	37.65508868555861	21.147582245121555
46-47	21.973734861271506	21.424447010748867	35.30210831778745	21.299709810192176
48-49	21.998534245358446	21.848997660714815	33.2245121553996	22.927955938527138
50-51	20.02309673980634	22.31389357732966	34.40747979035267	23.255529892511326
52-53	20.81075478961239	23.191865802019485	32.79996150543366	23.19741790293447
54-55	19.601877350389387	22.803588878031448	31.893118355985905	25.70141541559326
56-57	21.387803144709956	22.372375706967517	30.393680968878623	25.8461401794439
58-59	20.804462408575404	22.768425572236534	31.38343549199017	25.04367652719789
60-61	20.37917147848746	24.64059400076989	30.864499126469457	24.115735394273194
62-63	19.078499304136685	25.393829024903024	30.87116164756744	24.65651002339285
64-65	18.624337449290813	26.031210209943445	30.233780462527022	25.110671878238726
66-67	19.4353143229398	24.670575345710816	29.550501909922716	26.343608421426666
68-69	19.658211402576924	24.769864565250383	30.098790747944793	25.473133284227906
70-71	20.669437792180393	24.30792805931146	29.975533651408202	25.047100497099944
72-73	20.593188657385987	23.233037343568977	31.440553435467695	24.733220563577344
74-75	20.569945885122483	24.947254650844293	29.38193555073547	25.10086391329775
76-77	20.85850285748127	23.425424180509903	29.309540730212312	26.406532231796515
78-79	21.54807379112256	23.64898877735335	29.719655917799294	25.083281513724792
80-81	21.676512392289244	23.143377454028606	29.37838678155814	25.801723372124012
82-83	21.051793890445538	22.838836719510525	30.325317303741762	25.78405208630218
84-85	21.13277664268159	23.664164519854314	29.360990198691184	25.842068638772915
86-87	21.585537834006374	24.589975829764555	27.991575581029508	25.832910755199563
88-89	21.082905812068315	24.530837053323562	27.22329565223829	27.162961482369834
90-91	22.055655495591534	23.10761691133468	27.773706146682358	27.06302144639142
92-93	22.515129642995944	24.0545592508282	27.130087168952304	26.30022393722355
94-95	22.01905495140025	23.744290547293147	27.532331973675443	26.70432252763116
96-97	21.08510661923921	23.87116117068332	28.588243567867284	26.45548864221019
98-99	22.13001587906739	24.24759500605181	26.683865905162364	26.938523209718433
100	22.126972846525124	22.983476947677	26.352491782890645	28.53705842290723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	100.5
2	138.0
3	250.0
4	174.5
5	85.5
6	170.5
7	355.5
8	543.0
9	569.5
10	377.0
11	216.5
12	171.5
13	146.5
14	156.5
15	151.5
16	151.0
17	161.0
18	162.0
19	165.0
20	199.0
21	275.0
22	315.0
23	355.5
24	455.5
25	571.5
26	756.5
27	1068.5
28	1462.0
29	1795.0
30	2006.5
31	2327.5
32	2715.5
33	3027.5
34	3326.0
35	3524.0
36	3482.5
37	3338.5
38	3307.0
39	3441.0
40	3509.5
41	3334.5
42	3161.5
43	3226.0
44	3231.0
45	3353.5
46	3868.0
47	5272.5
48	7969.5
49	8144.5
50	6343.0
51	5209.5
52	3879.5
53	3286.0
54	3153.5
55	3019.0
56	2890.0
57	2582.5
58	2072.5
59	1781.5
60	1791.5
61	1678.5
62	1845.5
63	1937.5
64	1672.5
65	1375.0
66	937.0
67	523.5
68	372.0
69	288.5
70	185.5
71	220.0
72	237.0
73	203.0
74	195.5
75	137.0
76	53.0
77	22.0
78	15.5
79	13.0
80	10.5
81	7.0
82	5.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04293624707589352
2	0.23985075952740517
3	0.002220840365994492
4	0.0029611204879926565
5	7.402801219981641E-4
6	0.0
7	0.0
8	7.402801219981641E-4
9	0.0
10-11	3.7014006099908206E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.7014006099908206E-4
20-21	0.0
22-23	0.0
24-25	0.0014805602439963283
26-27	3.7014006099908206E-4
28-29	3.7014006099908206E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	3.7014006099908206E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.7014006099908206E-4
70-71	3.7014006099908206E-4
72-73	3.7014006099908206E-4
74-75	7.402801219981641E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.7014006099908206E-4
84-85	0.0
86-87	3.7014006099908206E-4
88-89	0.002220840365994492
90-91	0.002220840365994492
92-93	0.001110420182997246
94-95	7.402801219981641E-4
96-97	3.7014006099908206E-4
98-99	3.7014006099908206E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	135084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.163157738888394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57669480591292	31.558881880903733
2	8.849117115788697	7.108169731426371
3	3.354591366535186	4.041929466109976
4	2.023813912338261	3.251310295815937
5	1.3123456335016774	2.6353972343134644
6	0.9400228554576621	2.2652571733143825
7	0.7778228333394772	2.186787480382577
8	0.5271500718841007	1.6937609191317995
9	0.46632506358978143	1.6856178377898199
>10	2.899325395362554	21.352639838915046
>50	0.16404320418770968	4.443161292232981
>100	0.08478637519814207	7.3339551686358115
>500	0.012902274486673793	3.169879482396139
>1k	0.011059092417148966	7.273252198631962
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2059	1.52423677119422	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	1.4983269669242842	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1809	1.3391667406946788	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	1744	1.2910485327647983	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1123	0.8313345770039383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1066	0.7891386100500429	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	838	0.6203547422344615	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	731	0.541144769180658	TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	607	0.4493500340528856	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	584	0.4323235912469278	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	510	0.3775428622190637	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	509	0.3768025820970655	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	503	0.37236090136507655	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	480	0.35533445855911877	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	460	0.3405288561191555	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	447	0.3309052145331794	RNA PCR Primer, Index 3 (95% over 23bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	439	0.324982973557194	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.3116579313612271	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.29018980782328035	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	383	0.28352728672529687	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	354	0.26205916318735006	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	325	0.24059103964940332	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	320	0.23688963903941251	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	296	0.2191229161114566	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	277	0.20505759379349145	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	268	0.198395072695508	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	264	0.19543395220751533	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	264	0.19543395220751533	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	245	0.1813686298895502	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	220	0.1628616268395961	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	213	0.15767966598560895	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	207	0.15323798525361998	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	202	0.14953658464362915	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	194	0.14361434366764383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	191	0.14139350330164935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	188	0.13917266293565483	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	184	0.1362115424476622	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	156	0.1154836990317136	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.11474341890971543	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	154	0.11400313878771728	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	148	0.1095614580557283	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	147	0.10882117793373013	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143	0.10586005744573747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	7.402801219981641E-4	0.0
18-19	0.0	0.0	0.0	0.0033312605489917383	0.0
20-21	0.0	0.0	0.0	0.004441680731988985	0.0
22-23	0.0	0.0	0.0	0.011474341890971544	0.0
24-25	0.0	0.0	0.0	0.026279944330934828	0.0
26-27	0.0	0.0	0.0	0.05478072902786414	0.0
28-29	0.0	0.0	0.0	0.1299191614106778	0.0
30-31	0.0	0.0	0.0	0.28093630629830324	0.0
32-33	0.0	0.0	0.0	0.49228628112877915	0.0
34-35	0.0	0.0	0.0	0.8483610198098961	0.0
36-37	0.0	0.0	0.0	1.3376861804506825	0.0
38-39	0.0	0.0	0.0	1.904000473779278	0.0
40-41	0.0	0.0	0.0	2.5184329750377543	0.0
42-43	0.0	0.0	0.0	2.957048947321667	0.0
44-45	0.0	0.0	0.0	3.3656835746646534	0.0
46-47	0.0	0.0	0.0	3.8446448135974656	0.0
48-49	0.0	0.0	0.0	4.278819105149388	0.0
50-51	0.0	0.0	0.0	4.685232892126381	0.0
52-53	0.0	0.0	0.0	5.065366734772438	0.0
54-55	0.0	0.0	0.0	5.486956264250392	0.0
56-57	0.0	0.0	0.0	5.97554114476918	0.0
58-59	0.0	0.0	0.0	6.378623671197181	0.0
60-61	0.0	0.0	0.0	6.828343845311066	0.0
62-63	0.0	0.0	0.0	7.227354831068077	0.0
64-65	0.0	0.0	0.0	7.6474638003020345	0.0
66-67	0.0	0.0	0.0	8.159367504663765	0.0
68-69	0.0	0.0	0.0	8.757143703177283	0.0
70-71	0.0	0.0	0.0	9.380829705960736	0.0
72-73	0.0	0.0	0.0	9.971203103254272	0.0
74-75	0.0	0.0	0.0	10.606733587989694	0.0
76-77	0.0	0.0	0.0	11.267063456812057	0.0
78-79	0.0	0.0	0.0	12.02066862100619	0.0
80-81	0.0	0.0	0.0	12.77464392526132	0.0
82-83	0.0	0.0	0.0	13.49160522341654	0.0
84-85	0.0	0.0	0.0	14.228554084865713	0.0
86-87	0.0	0.0	0.0	14.936261881495959	0.0
88	0.0	0.0	0.0	15.534038080009475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGTG	15	6.891075E-4	93.99259	5
AACGTGT	20	0.002159512	70.49444	6
TAAACGT	20	0.002159512	70.49444	4
AAATTCG	20	0.002159512	70.49444	94
CGTGTTA	20	0.002159512	70.49444	8
GTATTAA	40	7.808143E-6	58.98559	1
CTCTGAA	25	0.005166654	56.56309	2
TTAAACG	25	0.005220039	56.416443	3
CTCTATC	55	5.2607153E-5	42.723907	9
TGTTGTA	45	0.0010111684	41.774487	94
GAGCGTG	45	0.0010111684	41.774487	9
CCCTATG	60	8.634497E-5	39.279926	2
TATTAAT	60	8.634497E-5	39.279926	2
CTATGTT	75	7.398985E-6	37.59704	4
ATGGGAT	50	0.0016973715	37.59704	5
GGGAGCG	50	0.0016973715	37.59704	7
ACGGGAG	50	0.0016973715	37.59704	5
GGTAGTA	25	0.0017003812	37.59704	30-31
ACTTAGA	90	5.9469494E-7	36.70215	1
ACACGGG	55	0.0027046932	34.191788	3
>>END_MODULE