FastQCFastQC Report
Wed 25 May 2016
SRR1033052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140727
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.034.338431
GGTATCA25000.033.4749221
TCAACGC37850.031.9206814
ATCAACG39000.031.2205163
CAACGCA39550.030.6674865
AACGCAG40900.029.763066
TATCAAC47100.026.3504472
GTACATG82850.025.5363941
TACATGG82550.024.431222
ACGCAGA50150.023.8984857
CGCAGAG51550.022.9997568
ACATGGG86500.021.956863
GAGTACA63500.018.6580561
GCAGAGT63950.017.880499
CATGGGG61100.017.4658454
AGTACAT65600.017.1276992
GTATAGG11400.016.9090251
GTATAGA13250.016.3223061
AATACCG2607.3143874E-616.2732435
ATTATAC18600.016.1760313
Produced by FastQC (version 0.11.5)