FastQCFastQC Report
Wed 25 May 2016
SRR1033052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140727
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38500.12258308347080149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32570.10370210464010403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.052.6352731
GTATCAA73500.044.312561
TCAACGC87250.036.5207944
ATCAACG88500.035.952433
CAACGCA89550.035.5827985
TATCAAC94050.034.013092
AACGCAG93750.033.9886866
ACGCAGA108100.029.302877
CGCAGAG108400.028.9616378
GTACATG81450.026.8511581
GCAGAGT123200.025.1010069
TACATGG82200.024.8744222
ACATGGG84050.023.9325473
AGAGTAC111600.021.41407210-11
GAGTACT80550.020.77097712-13
CAGAGTA121050.020.28588510-11
AGTACTT85250.019.7912212-13
CATGGGG60950.019.04584
GTGGTAT18950.018.9034251
GTACTTT91900.018.25696814-15
Produced by FastQC (version 0.11.5)