##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033052_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3140727 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70249881635685 34.0 31.0 34.0 31.0 34.0 2 32.694034215644976 34.0 31.0 34.0 31.0 34.0 3 32.802333026716425 34.0 31.0 34.0 31.0 34.0 4 36.300041996646 37.0 37.0 37.0 35.0 37.0 5 36.28471783762167 37.0 37.0 37.0 35.0 37.0 6 36.34966331043736 37.0 37.0 37.0 35.0 37.0 7 36.29572388813163 37.0 37.0 37.0 35.0 37.0 8 36.29744068809546 37.0 37.0 37.0 35.0 37.0 9 38.097565627321316 39.0 39.0 39.0 35.0 39.0 10-11 38.06069215821687 39.0 39.0 39.0 35.0 39.0 12-13 38.045714256603645 39.0 39.0 39.0 35.0 39.0 14-15 39.542954863635075 41.0 39.0 41.0 37.0 41.0 16-17 39.538424065510945 41.0 39.0 41.0 37.0 41.0 18-19 39.55028549122544 41.0 39.5 41.0 37.0 41.0 20-21 39.540701086086116 41.0 40.0 41.0 37.0 41.0 22-23 39.47592993596706 41.0 39.0 41.0 36.5 41.0 24-25 39.427248691146985 41.0 39.0 41.0 36.0 41.0 26-27 39.32566982103188 41.0 39.0 41.0 36.0 41.0 28-29 39.27453755133764 41.0 39.0 41.0 36.0 41.0 30-31 39.275280691381326 41.0 39.0 41.0 36.0 41.0 32-33 39.244771990688776 41.0 39.0 41.0 35.5 41.0 34-35 39.17225454488722 41.0 39.0 41.0 35.0 41.0 36-37 39.08922663447029 41.0 39.0 41.0 35.0 41.0 38-39 38.99909081559779 41.0 39.0 41.0 35.0 41.0 40-41 38.90299347889836 41.0 39.0 41.0 35.0 41.0 42-43 38.79484320031636 41.0 39.0 41.0 35.0 41.0 44-45 38.64626438401045 41.0 39.0 41.0 35.0 41.0 46-47 38.47341968913567 41.0 38.0 41.0 35.0 41.0 48-49 38.29573041528283 40.5 38.0 41.0 34.0 41.0 50-51 38.16543462707838 40.0 38.0 41.0 34.0 41.0 52-53 38.03410802658111 40.0 38.0 41.0 34.0 41.0 54-55 37.89118172321249 40.0 38.0 41.0 34.0 41.0 56-57 37.747185763041486 40.0 37.0 41.0 33.0 41.0 58-59 37.57045040845639 40.0 37.0 41.0 33.0 41.0 60-61 37.3775745233508 40.0 36.5 41.0 33.0 41.0 62-63 37.144152611799754 39.0 36.0 41.0 33.0 41.0 64-65 36.87730356697669 39.0 35.0 41.0 32.0 41.0 66-67 36.59990314344418 39.0 35.0 41.0 32.0 41.0 68-69 36.29263988879008 38.0 35.0 40.5 32.0 41.0 70-71 35.960522675164064 37.0 35.0 40.0 31.0 41.0 72-73 35.608608611955134 37.0 35.0 39.5 31.0 41.0 74-75 35.25598484045254 36.0 35.0 39.0 31.0 41.0 76-77 34.89603235174531 36.0 35.0 39.0 31.0 40.5 78-79 34.530843336590536 35.0 35.0 37.5 30.5 39.5 80-81 34.19477799248391 35.0 34.0 37.0 30.0 39.0 82-83 33.890003652020695 35.0 34.0 37.0 30.0 39.0 84-85 33.663542230827446 35.0 34.0 36.0 30.0 37.0 86-87 33.4522944846846 35.0 34.0 36.0 30.0 37.0 88-89 33.241701841643675 35.0 34.0 35.5 30.0 37.0 90-91 33.058527850398974 35.0 34.0 35.0 30.0 36.0 92-93 32.88770370681692 35.0 34.0 35.0 30.0 36.0 94-95 32.732199901487775 35.0 34.0 35.0 29.0 36.0 96-97 32.592735057838524 35.0 34.0 35.0 29.0 36.0 98-99 32.468769173506644 35.0 34.0 35.0 29.0 35.5 100 32.363060208671435 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15489493630136053 1101 2 0.14919368698425473 1101 3 0.1765357484094494 1101 4 0.1419658970333657 1101 5 0.11567413695216544 1101 6 0.11942065054171991 1101 7 0.1964683931992326 1101 8 0.1753500924725202 1101 9 0.22922958878640287 1101 10-11 0.22119142374712908 1101 12-13 0.22266215610110862 1101 14-15 0.28947243215559837 1101 16-17 0.34611181387580814 1101 18-19 0.3217973780562886 1101 20-21 0.310565053593983 1101 22-23 0.2638476882220644 1101 24-25 0.3562224864975079 1101 26-27 0.30658860865777626 1101 28-29 0.32989694249696555 1101 30-31 0.32348026396332585 1101 32-33 0.32412543140449657 1101 34-35 0.3515561902348452 1101 36-37 0.34995565180700794 1101 38-39 0.3256091503979732 1101 40-41 0.35668450554841513 1101 42-43 0.27353248771593996 1101 44-45 0.34575464685109836 1101 46-47 0.3102888133055757 1101 48-49 0.3952803985960003 1101 50-51 0.4079473413017851 1101 52-53 0.40464571403322225 1101 54-55 0.4055941310366791 1101 56-57 0.36944414591664554 1101 58-59 0.31617854801047685 1101 60-61 0.38502976823960466 1101 62-63 0.37780741565401854 1101 64-65 0.4100295083368124 1101 66-67 0.3971364142319658 1101 68-69 0.3927999584230335 1101 70-71 0.35771124679917676 1101 72-73 0.32544527036689175 1101 74-75 0.3310434502465398 1101 76-77 0.3425764172129817 1101 78-79 0.2859345873827053 1101 80-81 0.30760489046136286 1101 82-83 0.29650173026831084 1101 84-85 0.32182075330216975 1101 86-87 0.3535478043126332 1101 88-89 0.3607812725110904 1101 90-91 0.3842685456729029 1101 92-93 0.36665621914467295 1101 94-95 0.37427600945746065 1101 96-97 0.4057412422639928 1101 98-99 0.4239805450614469 1101 100 0.4080135658899877 1102 1 0.13952816079622465 1102 2 0.06282778953568169 1102 3 0.12906899862516497 1102 4 0.09838981210135245 1102 5 0.13096220412539594 1102 6 0.13035685143295694 1102 7 0.1432752448279473 1102 8 0.18283270555873088 1102 9 0.23805308167769113 1102 10-11 0.22553353384503083 1102 12-13 0.23505960530243186 1102 14-15 0.34755421431901823 1102 16-17 0.2802596998880986 1102 18-19 0.28385758004730377 1102 20-21 0.31245810807783414 1102 22-23 0.36013483932762114 1102 24-25 0.34644103769149837 1102 26-27 0.3626174869626908 1102 28-29 0.3161195380727335 1102 30-31 0.31176863649244524 1102 32-33 0.3018851327892378 1102 34-35 0.28798763150197715 1102 36-37 0.2678129150326498 1102 38-39 0.28163633087343953 1102 40-41 0.2399058708313504 1102 42-43 0.24120368657358426 1102 44-45 0.22257699434103984 1102 46-47 0.2580427750131946 1102 48-49 0.2624618370123102 1102 50-51 0.2848531611823546 1102 52-53 0.251942174058847 1102 54-55 0.20978984769175213 1102 56-57 0.27805634773535104 1102 58-59 0.29195194099590793 1102 60-61 0.20199371977291491 1102 62-63 0.20345095002809188 1102 64-65 0.2290267499974874 1102 66-67 0.2522292563604367 1102 68-69 0.260722385356182 1102 70-71 0.21076177749758074 1102 72-73 0.19433510530999598 1102 74-75 0.28246599057948885 1102 76-77 0.19924373006845997 1102 78-79 0.2209428421280748 1102 80-81 0.20392209568206 1102 82-83 0.1921693256730279 1102 84-85 0.17360647709066512 1102 86-87 0.2523664697933583 1102 88-89 0.25579168121917917 1102 90-91 0.17238487993244433 1102 92-93 0.21357844715302576 1102 94-95 0.2764982401265925 1102 96-97 0.21894123263450638 1102 98-99 0.2580733909910222 1102 100 0.33063329137377906 1103 1 0.14294813678132812 1103 2 0.14072807930083542 1103 3 0.13022900792440595 1103 4 0.11918410720877404 1103 5 0.12123638609657661 1103 6 0.15171899899106478 1103 7 0.1538623285613383 1103 8 0.1714748741095775 1103 9 0.18208724202236226 1103 10-11 0.2131214441235656 1103 12-13 0.23334118167883133 1103 14-15 0.28360966140404287 1103 16-17 0.2891855755824011 1103 18-19 0.31244703397296547 1103 20-21 0.3105716075619682 1103 22-23 0.3276316438127722 1103 24-25 0.27621817467646537 1103 26-27 0.2641838198261368 1103 28-29 0.31452544831545737 1103 30-31 0.2652610953317023 1103 32-33 0.26143278540007486 1103 34-35 0.30023115530909195 1103 36-37 0.3261447658140142 1103 38-39 0.3588237731382691 1103 40-41 0.38481189398928706 1103 42-43 0.4112482081226858 1103 44-45 0.40287469534060705 1103 46-47 0.4046945581295489 1103 48-49 0.39565678361452683 1103 50-51 0.4093666942329648 1103 52-53 0.37372219588292666 1103 54-55 0.39250634975195453 1103 56-57 0.41389074216511546 1103 58-59 0.3912070429878227 1103 60-61 0.3847899324337476 1103 62-63 0.33653838512385903 1103 64-65 0.3217127693547397 1103 66-67 0.32454525617539787 1103 68-69 0.301609008812207 1103 70-71 0.3525877439250422 1103 72-73 0.34597863031032006 1103 74-75 0.3527513995198106 1103 76-77 0.3948686059179778 1103 78-79 0.33655213871185197 1103 80-81 0.37143871534229334 1103 82-83 0.3458807294823245 1103 84-85 0.39013685167082457 1103 86-87 0.39296137201191783 1103 88-89 0.3274161548969303 1103 90-91 0.3449893343172974 1103 92-93 0.38217416740774013 1103 94-95 0.39547824371992846 1103 96-97 0.3611583678316137 1103 98-99 0.401494718633181 1103 100 0.42152513758571786 1104 1 0.15003785918107582 1104 2 0.1321917988756809 1104 3 0.13025188936387622 1104 4 0.08589250548639882 1104 5 0.10859281681003097 1104 6 0.11572682359764741 1104 7 0.14751430308861302 1104 8 0.10366697333176944 1104 9 0.14066027840253525 1104 10-11 0.1545227119338506 1104 12-13 0.16119929194632476 1104 14-15 0.22907165646070382 1104 16-17 0.21938919784113153 1104 18-19 0.2366452885372894 1104 20-21 0.2286245746644724 1104 22-23 0.24348818066415134 1104 24-25 0.24541381361505188 1104 26-27 0.21623585738777962 1104 28-29 0.1938992213746218 1104 30-31 0.25273623232198617 1104 32-33 0.30384195947596027 1104 34-35 0.22998201167163046 1104 36-37 0.2466989749511299 1104 38-39 0.22440024962775595 1104 40-41 0.2533178290212206 1104 42-43 0.24669819206809507 1104 44-45 0.21781684852447114 1104 46-47 0.15924721283226972 1104 48-49 0.13736564468922552 1104 50-51 0.25680457911216337 1104 52-53 0.2660884919266522 1104 54-55 0.19045464108162946 1104 56-57 0.28169296721830506 1104 58-59 0.2565773515997094 1104 60-61 0.2630665005335686 1104 62-63 0.20932582745159323 1104 64-65 0.20534035236151027 1104 66-67 0.2198580661261076 1104 68-69 0.2568966792495644 1104 70-71 0.22857769507340464 1104 72-73 0.23022638489875646 1104 74-75 0.16112514654957266 1104 76-77 0.1959610983344504 1104 78-79 0.09956242182808239 1104 80-81 0.11973677320142428 1104 82-83 0.10461782155488919 1104 84-85 0.13185351216842633 1104 86-87 0.1668157659953522 1104 88-89 0.12655756999227208 1104 90-91 0.18334530805973515 1104 92-93 0.16253605217305278 1104 94-95 0.19036488568591636 1104 96-97 0.24974476283779978 1104 98-99 0.22354529403795453 1104 100 0.26822151347580103 1105 1 0.10182021595939972 1105 2 0.14119906880156918 1105 3 0.17722157291696305 1105 4 0.09285014628770938 1105 5 0.09814631998158063 1105 6 0.14749135238070465 1105 7 0.1394037459047226 1105 8 0.13582112055441087 1105 9 0.1818736446763367 1105 10-11 0.19728805832530583 1105 12-13 0.1883143740170823 1105 14-15 0.21970074265788497 1105 16-17 0.21042679306889767 1105 18-19 0.25372858128537246 1105 20-21 0.24055427805267726 1105 22-23 0.2619584560240398 1105 24-25 0.24279687642922454 1105 26-27 0.2601960336606055 1105 28-29 0.26746774256602635 1105 30-31 0.1999317174707329 1105 32-33 0.16620540854368215 1105 34-35 0.2441446419502995 1105 36-37 0.25818209296123484 1105 38-39 0.15929825519457808 1105 40-41 0.1917858611234209 1105 42-43 0.2211223979991388 1105 44-45 0.16176079069564508 1105 46-47 0.1468546899643286 1105 48-49 0.12381105530869263 1105 50-51 0.11083816304099514 1105 52-53 0.13864046318933987 1105 54-55 0.15240367780907604 1105 56-57 0.17122669430240478 1105 58-59 0.12376262402268878 1105 60-61 0.14243708965435786 1105 62-63 0.08615209009573732 1105 64-65 0.03755559883945381 1105 66-67 0.05725464751945708 1105 68-69 0.13306000021546538 1105 70-71 0.0937971653694234 1105 72-73 0.0349879944951752 1105 74-75 0.050821509815641264 1105 76-77 0.050829410253527385 1105 78-79 0.056315660531360834 1105 80-81 0.043866551561663414 1105 82-83 0.018073530498107004 1105 84-85 -0.042110235606763524 1105 86-87 -0.02892056858062375 1105 88-89 0.007092882115017574 1105 90-91 0.05543593567011129 1105 92-93 0.07769811278233618 1105 94-95 -0.016823869689780224 1105 96-97 0.04960662539104277 1105 98-99 0.061093295324880614 1105 100 0.09474036126781726 1106 1 -0.034030498153285293 1106 2 9.487368710452415E-4 1106 3 -0.008300950737847756 1106 4 0.030013491764648847 1106 5 0.02379336161412482 1106 6 -6.623479209366678E-5 1106 7 0.02110088175639646 1106 8 0.024920709278035247 1106 9 0.02652028925344041 1106 10-11 0.04247931684319184 1106 12-13 0.02448764674981163 1106 14-15 0.0476743856567694 1106 16-17 0.08294051061678687 1106 18-19 0.10076566922076324 1106 20-21 0.08398470658987378 1106 22-23 0.11863996841820779 1106 24-25 0.10390027305803784 1106 26-27 0.1102447197028269 1106 28-29 0.09260917997630713 1106 30-31 0.08446249405115935 1106 32-33 0.07603509654855856 1106 34-35 0.0913453576068477 1106 36-37 0.03994224451203365 1106 38-39 0.036811283440314924 1106 40-41 0.06070718369639394 1106 42-43 0.04250576872585299 1106 44-45 -0.02832217345150667 1106 46-47 0.007444741311900316 1106 48-49 0.01803668195393726 1106 50-51 -0.00833713584868434 1106 52-53 -0.008776154080962328 1106 54-55 0.006432681556532316 1106 56-57 -0.04231594044965448 1106 58-59 -0.007527671342188569 1106 60-61 -0.09562989398983035 1106 62-63 -0.0808623008807956 1106 64-65 -0.02545055443264488 1106 66-67 0.027320385635960065 1106 68-69 -0.04881150516391841 1106 70-71 -0.09516847694059294 1106 72-73 -0.11483667921701368 1106 74-75 -0.08277268560782858 1106 76-77 -0.05278731687023708 1106 78-79 -0.06819033596254798 1106 80-81 -0.07660152592025327 1106 82-83 -0.09393767925109842 1106 84-85 -0.08665815993747827 1106 86-87 -0.08371426283576255 1106 88-89 -0.02769591526798365 1106 90-91 -0.1070024892070478 1106 92-93 -0.010055705886401256 1106 94-95 -0.03536287962573681 1106 96-97 -0.09604472601085234 1106 98-99 -0.05070373062984146 1106 100 -0.07997694874028127 1107 1 -0.062460068815966 1107 2 -0.07707896810760673 1107 3 -0.03344098264839346 1107 4 0.008002741819886694 1107 5 -0.017750528126519782 1107 6 0.014774113575313663 1107 7 -0.009108947348849483 1107 8 -0.020454843113363097 1107 9 0.02023901110163706 1107 10-11 0.03845424805513886 1107 12-13 0.027464760203237404 1107 14-15 0.07149653180358229 1107 16-17 0.07293808162010151 1107 18-19 0.0666508801009229 1107 20-21 0.10132055988853494 1107 22-23 0.13163128705999583 1107 24-25 0.09810722491696566 1107 26-27 0.10810430257251369 1107 28-29 0.14558188469177225 1107 30-31 0.14172433084203107 1107 32-33 0.09432913791291497 1107 34-35 0.12281670600678041 1107 36-37 0.143515065631604 1107 38-39 0.1503013761847285 1107 40-41 0.1327617611900962 1107 42-43 0.12095502783995471 1107 44-45 0.070065183964374 1107 46-47 0.07113558250244978 1107 48-49 0.04328450164024389 1107 50-51 0.0647179150395445 1107 52-53 0.0765729056508988 1107 54-55 0.08191802828616801 1107 56-57 0.0822069771089673 1107 58-59 0.10337216213155642 1107 60-61 0.11592339658824358 1107 62-63 0.1194403262911834 1107 64-65 0.10270190274308533 1107 66-67 0.0984471165685008 1107 68-69 0.07768960305534733 1107 70-71 0.09852100139674036 1107 72-73 0.04973689859620123 1107 74-75 0.06494379767433855 1107 76-77 0.09148867448394782 1107 78-79 0.1290182959105195 1107 80-81 0.14728885508522893 1107 82-83 0.10808259557922639 1107 84-85 0.11811954579552975 1107 86-87 0.13792254901975554 1107 88-89 0.07925713385206024 1107 90-91 0.07241458008524404 1107 92-93 0.10822620725777199 1107 94-95 0.16044312819331452 1107 96-97 0.1245036053237385 1107 98-99 0.1386271738185556 1107 100 0.09245063648162954 1108 1 0.052522554518525055 1108 2 0.045560064806842604 1108 3 0.06773417884162569 1108 4 0.06096843123141582 1108 5 0.07846843091913769 1108 6 0.05910909859520075 1108 7 0.06753938707718987 1108 8 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-0.16228316832466305 2214 64-65 -0.23240390196672678 2214 66-67 -0.1892495991828369 2214 68-69 -0.220098048205827 2214 70-71 -0.13235718861130152 2214 72-73 -0.10790052480859202 2214 74-75 -0.13145279287457612 2214 76-77 -0.1582022908395686 2214 78-79 -0.005556697181894776 2214 80-81 -0.16203439680504061 2214 82-83 -0.11771429943066636 2214 84-85 -0.05275174791521664 2214 86-87 -0.1510618206278096 2214 88-89 -0.10179916658558597 2214 90-91 -0.07267736050722107 2214 92-93 -0.09490716607810157 2214 94-95 -0.10795340363166162 2214 96-97 -0.1444632261965566 2214 98-99 -0.15848470053632724 2214 100 -0.1674074925288238 2215 1 -0.20468959313622292 2215 2 -0.19315670919073824 2215 3 -0.18650139319350245 2215 4 -0.15280951958987288 2215 5 -0.19347183671617074 2215 6 -0.20325837034233984 2215 7 -0.24180542703932417 2215 8 -0.2051023270529555 2215 9 -0.2620953631325733 2215 10-11 -0.2549399250795048 2215 12-13 -0.28866538540331277 2215 14-15 -0.40790785478309743 2215 16-17 -0.4030512131600972 2215 18-19 -0.41073198373722164 2215 20-21 -0.3822716477794401 2215 22-23 -0.3737172172106895 2215 24-25 -0.39629443265373965 2215 26-27 -0.40939102835813657 2215 28-29 -0.48710185429320774 2215 30-31 -0.4828320716216865 2215 32-33 -0.43184877379804476 2215 34-35 -0.42489891993307083 2215 36-37 -0.4289795864506516 2215 38-39 -0.45407094105860324 2215 40-41 -0.42870555865435733 2215 42-43 -0.45646337526341085 2215 44-45 -0.44924366360229584 2215 46-47 -0.45083367724401313 2215 48-49 -0.443462705053733 2215 50-51 -0.40285224057490865 2215 52-53 -0.3867022283516519 2215 54-55 -0.3379859392610953 2215 56-57 -0.3668106187626705 2215 58-59 -0.37333419938177315 2215 60-61 -0.40273418788902404 2215 62-63 -0.33258541182591017 2215 64-65 -0.3612196508374339 2215 66-67 -0.4099462914426937 2215 68-69 -0.39320017421427167 2215 70-71 -0.36975901244358766 2215 72-73 -0.386987203034586 2215 74-75 -0.3727547768348387 2215 76-77 -0.3643716300185389 2215 78-79 -0.39235483352914713 2215 80-81 -0.319675823758935 2215 82-83 -0.36454528333964475 2215 84-85 -0.3037778672698792 2215 86-87 -0.34344499221858626 2215 88-89 -0.3139519528175754 2215 90-91 -0.2981067989472308 2215 92-93 -0.2843126963558973 2215 94-95 -0.2565101192070429 2215 96-97 -0.2910312500225203 2215 98-99 -0.2637667998239621 2215 100 -0.3486716384188284 2216 1 -0.3094097602344945 2216 2 -0.26977080329499614 2216 3 -0.2597269617321345 2216 4 -0.21123266544143604 2216 5 -0.2079585429710633 2216 6 -0.19261268497385942 2216 7 -0.22388841534781534 2216 8 -0.2555702521384262 2216 9 -0.3374212508610839 2216 10-11 -0.3310157103564251 2216 12-13 -0.3373546357000805 2216 14-15 -0.4535742575509829 2216 16-17 -0.4065009880923469 2216 18-19 -0.3623668967549847 2216 20-21 -0.44161485987425664 2216 22-23 -0.4864594225659218 2216 24-25 -0.4645687266354699 2216 26-27 -0.49541928647501265 2216 28-29 -0.5225965251248326 2216 30-31 -0.4436157536692136 2216 32-33 -0.4856023277859194 2216 34-35 -0.5268946455075749 2216 36-37 -0.5453876440491428 2216 38-39 -0.48033510128149715 2216 40-41 -0.4841707047912962 2216 42-43 -0.43480814465272033 2216 44-45 -0.4755270660504465 2216 46-47 -0.37705648562179306 2216 48-49 -0.4064401522945218 2216 50-51 -0.4333954285184589 2216 52-53 -0.5442446143716779 2216 54-55 -0.5071218796356476 2216 56-57 -0.45676459385982326 2216 58-59 -0.4582259919780114 2216 60-61 -0.5258435777048049 2216 62-63 -0.5146325065661728 2216 64-65 -0.5975228511869162 2216 66-67 -0.597085644130452 2216 68-69 -0.5677631986193603 2216 70-71 -0.5606566406612998 2216 72-73 -0.518469631265539 2216 74-75 -0.629755641939532 2216 76-77 -0.5951171795058485 2216 78-79 -0.613520747230119 2216 80-81 -0.5680206041329825 2216 82-83 -0.5408922097521085 2216 84-85 -0.6652486179700716 2216 86-87 -0.6993131304003342 2216 88-89 -0.850143964922907 2216 90-91 -0.8167221935683315 2216 92-93 -0.8435697350993934 2216 94-95 -0.7032301209282323 2216 96-97 -0.8061261507457083 2216 98-99 -0.9037242729910062 2216 100 -0.8773809438112394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 5.0 4 10.0 5 26.0 6 58.0 7 156.0 8 369.0 9 696.0 10 1221.0 11 2065.0 12 3141.0 13 4818.0 14 5801.0 15 7665.0 16 8929.0 17 12258.0 18 14316.0 19 7591.0 20 5436.0 21 5328.0 22 5447.0 23 6114.0 24 7089.0 25 8952.0 26 11255.0 27 14747.0 28 19133.0 29 25156.0 30 32413.0 31 41393.0 32 53172.0 33 70206.0 34 96425.0 35 144935.0 36 262293.0 37 599156.0 38 1118543.0 39 542730.0 40 1665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22672419422399 20.14420113621704 13.772785260713553 23.85628940884542 2 15.472801594997948 20.587820329073665 40.21052772700663 23.728850348921764 3 17.698022599859456 25.950992775674 30.532162403892965 25.818822220573583 4 12.113308920151319 17.259945622773355 39.533370926364675 31.093374530710648 5 12.389901802740074 37.96796211313397 36.24982050288485 13.39231558124111 6 30.193748382140033 38.85643528576064 17.869898109448048 13.079918222651282 7 26.641538726543253 32.756906283163104 23.196189926727158 17.405365063566492 8 24.92213573821561 36.133411362100816 20.791875243972562 18.15257765571101 9 25.15264777868309 16.193543724112285 21.482701298138934 37.171107199065695 10-11 23.586976456293986 26.668204534513194 29.48925275542616 20.255566253766663 12-13 24.67849004386564 24.09236778618454 29.47714016531841 21.752002004631414 14-15 21.86433165919385 25.915984816210813 26.85537192450898 25.36431160008635 16-17 20.960224916400605 29.392392992376752 27.378174926310745 22.269207164911894 18-19 20.799990320669224 27.999772663086375 29.96037842362546 21.239858592618937 20-21 22.136721848158086 27.301019795735193 29.447895343976093 21.114363012130628 22-23 21.68074384158422 27.158321055107542 29.567702972803232 21.593232130505 24-25 21.561036991493726 27.293821420605685 29.500332969134245 21.64480861876634 26-27 21.300435616049736 27.184348706803156 29.628959814845267 21.886255862301834 28-29 21.3279882753018 27.292415864448937 29.650011836456 21.729584023793265 30-31 21.643694779832543 27.69803322356031 29.20018734539786 21.45808465120929 32-33 21.27962729648263 27.358761204014233 29.736188468466057 21.62542303103708 34-35 21.479374043016154 27.160240288315414 29.688970101508342 21.67141556716009 36-37 21.82141268566163 27.514011883235952 29.055629476869527 21.60894595423289 38-39 21.632841695569212 27.70302862999554 29.14842646304502 21.515703211390232 40-41 21.5062218098618 27.443941305290558 29.28248118428669 21.76735570056095 42-43 21.539853670822072 27.309982688721433 29.19303396952362 21.957129670932876 44-45 21.712998296254337 27.539706571121908 29.003093869667758 21.744201262955997 46-47 21.762668324881467 27.811506698926713 28.688134944552644 21.737690031639172 48-49 21.763734956906475 27.707454356905263 28.533170823188392 21.995639862999873 50-51 21.83109197329153 27.66919888293379 28.33929851273288 22.160410631041792 52-53 21.82413498530754 27.635130337657493 28.40113133042127 22.139603346613697 54-55 21.92337952327598 28.046452302285424 28.221873470696433 21.80829470374216 56-57 21.74905682665192 27.698634742847755 28.322311999737643 22.229996430762686 58-59 21.565803076803554 27.36836089223928 28.735416991034242 22.330419039922923 60-61 21.89472373752956 27.77442929614704 28.261498054431343 22.069348911892057 62-63 21.668454469299625 27.772853227930987 28.320003616996956 22.238688685772434 64-65 21.7287430585339 27.504603233582543 28.466355082756316 22.30029862512724 66-67 21.574291807962265 27.570611449293658 28.360285430775335 22.494811311968743 68-69 22.05601834737947 27.738121278521717 28.203332928967583 22.00252744513123 70-71 22.029138799573918 27.53140077622168 28.196676787997937 22.242783636206465 72-73 21.811757116762116 27.64870082296442 28.274417418431895 22.265124641841567 74-75 21.886816542937236 27.691783805262737 28.176337685196152 22.24506196660388 76-77 21.883038544897406 27.745009356114046 27.99633651699113 22.375615581997415 78-79 22.035375885901576 27.8139742804771 28.03086673881557 22.119783094805754 80-81 21.88104855977613 27.66544179102482 28.165246454085313 22.288263195113743 82-83 21.783063111711964 27.647012655837848 28.277551883331377 22.29237234911881 84-85 21.711263029228583 27.64296291909485 28.108157760926055 22.537616290750517 86-87 21.91839376753581 27.750789947260436 27.988570122226907 22.34224616297685 88-89 21.602806926826503 27.685941071603125 28.072754794455555 22.638497207114813 90-91 21.604510390972088 27.54643213414764 28.118063756682915 22.73099371819736 92-93 21.705552482119902 27.76336579677382 27.92168974396733 22.609391977138944 94-95 21.4569384815437 27.518916068819976 28.111567553385914 22.912577896250408 96-97 21.44100259717269 27.51633939064349 28.123540344054536 22.91911766812928 98-99 21.42314615624516 27.580133182646232 28.027705072507953 22.969015588600655 100 21.509255659597283 27.57871027949898 27.947860479436766 22.964173581466966 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 13.5 2 63.5 3 115.0 4 85.5 5 71.5 6 145.0 7 330.0 8 599.0 9 704.0 10 605.5 11 557.5 12 671.5 13 820.5 14 966.5 15 1151.0 16 1350.0 17 1589.5 18 1901.0 19 2228.0 20 2685.0 21 3432.0 22 4560.5 23 6359.0 24 8714.5 25 11618.5 26 15685.5 27 21150.5 28 28109.0 29 36120.5 30 44418.5 31 53951.0 32 64445.0 33 75255.5 34 85868.0 35 95474.0 36 103650.0 37 110483.5 38 115379.0 39 118170.0 40 120855.0 41 122330.5 42 121038.5 43 119484.5 44 117616.0 45 115211.0 46 113210.5 47 109161.5 48 104832.0 49 101861.5 50 99022.0 51 95144.5 52 88601.0 53 83204.0 54 79466.0 55 74973.0 56 68783.0 57 61750.0 58 55951.5 59 50526.5 60 44424.5 61 37416.0 62 31005.0 63 25403.0 64 20448.5 65 16073.0 66 12414.0 67 9062.5 68 6392.5 69 4788.5 70 3335.0 71 2315.5 72 1700.5 73 1163.5 74 829.0 75 580.5 76 361.0 77 235.5 78 118.0 79 57.0 80 36.0 81 19.5 82 11.5 83 4.0 84 1.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04336575576291731 2 0.252584831473732 3 0.002801899050761177 4 0.0023561423835946264 5 2.547180955237434E-4 6 1.273590477618717E-4 7 0.0 8 1.5919880970233962E-4 9 0.0 10-11 4.7759642910701885E-4 12-13 0.0 14-15 2.865578574642113E-4 16-17 1.4327892873210564E-4 18-19 4.457566671665509E-4 20-21 0.0 22-23 1.4327892873210564E-4 24-25 0.0014487091682912905 26-27 7.959940485116981E-5 28-29 2.069584526130415E-4 30-31 1.273590477618717E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.1839761940467925E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5919880970233962E-5 68-69 1.9103857164280754E-4 70-71 1.5919880970233962E-5 72-73 2.547180955237434E-4 74-75 9.551928582140377E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.959940485116981E-5 84-85 0.0 86-87 3.502373813451471E-4 88-89 0.002165103811951819 90-91 0.002165103811951819 92-93 4.616765481367849E-4 94-95 3.502373813451471E-4 96-97 1.273590477618717E-4 98-99 7.959940485116981E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3140727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.11570089534636 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90421240845572 49.30767480372731 2 14.185549690673142 18.190329220065443 3 4.272588360122809 8.218199920397172 4 1.7454486603555592 4.4764265694216006 5 0.8923639855033033 2.860727119215449 6 0.49729087479382245 1.9130491791759512 7 0.3220876853295511 1.4455614386264757 8 0.22023825820689621 1.1296584231124336 9 0.16180758950856908 0.9336966310375558 >10 0.7474660210104268 8.246623028186043 >50 0.038195335782089376 1.6702264702785945 >100 0.012403746769088978 1.250858878035384 >500 1.985048514996465E-4 0.08040457667984273 >1k 1.488786375184979E-4 0.27656374204062917 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3850 0.12258308347080149 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3257 0.10370210464010403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.1839761940467925E-5 0.0 4 0.0 0.0 0.0 6.367952388093585E-5 0.0 5 0.0 0.0 0.0 6.367952388093585E-5 0.0 6 0.0 0.0 0.0 6.367952388093585E-5 0.0 7 0.0 0.0 0.0 6.367952388093585E-5 0.0 8 0.0 0.0 0.0 6.367952388093585E-5 0.0 9 0.0 0.0 0.0 9.551928582140377E-5 0.0 10-11 0.0 0.0 0.0 1.273590477618717E-4 0.0 12-13 0.0 0.0 0.0 1.4327892873210564E-4 0.0 14-15 0.0 0.0 0.0 3.502373813451472E-4 0.0 16-17 0.0 0.0 0.0 4.13916905226083E-4 0.0 18-19 0.0 0.0 0.0 4.13916905226083E-4 0.0 20-21 0.0 0.0 0.0 4.29836786196317E-4 0.0 22-23 0.0 0.0 0.0 4.7759642910701885E-4 0.0 24-25 0.0 0.0 0.0 6.049554768688905E-4 0.0 26-27 0.0 0.0 0.0 7.163946436605283E-4 0.0 28-29 0.0 0.0 0.0 0.0010666320250056756 0.0 30-31 0.0 0.0 0.0 0.0036138129802431094 0.0 32-33 0.0 0.0 0.0 0.010029525011247396 0.0 34-35 0.0 0.0 0.0 0.019024257759429584 0.0 36-37 0.0 0.0 0.0 0.03561277373041337 0.0 38-39 0.0 0.0 0.0 0.06296312923727532 0.0 40-41 0.0 0.0 0.0 0.09685655582290342 0.0 42-43 0.0 0.0 0.0 0.13259668860107865 0.0 44-45 0.0 0.0 0.0 0.17425901710018094 0.0 46-47 0.0 0.0 0.0 0.228561731089649 0.0 48-49 0.0 0.0 0.0 0.2869399346074969 0.0 50-51 0.0 0.0 0.0 0.35221144658545617 0.0 52-53 0.0 0.0 0.0 0.42163804749664646 0.0 54-55 0.0 0.0 0.0 0.49652516758062704 0.0 56-57 0.0 0.0 0.0 0.584100432797884 0.0 58-59 0.0 0.0 0.0 0.6841887244577449 0.0 60-61 0.0 0.0 0.0 0.7924757548172764 0.0 62-63 0.0 0.0 0.0 0.9050452331578007 0.0 64-65 0.0 0.0 0.0 1.0255428122215016 0.0 66-67 0.0 0.0 0.0 1.1678028685715123 0.0 68-69 0.0 0.0 0.0 1.3292941411335657 0.0 70-71 0.0 0.0 0.0 1.5053202650214426 0.0 72-73 0.0 0.0 0.0 1.6898794451093648 0.0 74-75 0.0 0.0 0.0 1.876126132580132 0.0 76-77 0.0 0.0 0.0 2.074408250064396 0.0 78-79 0.0 0.0 0.0 2.3128243874746195 0.0 80-81 0.0 0.0 0.0 2.576425776579754 0.0 82-83 0.0 0.0 0.0 2.852540192127491 0.0 84-85 0.0 0.0 0.0 3.129020764937545 0.0 86-87 0.0 0.0 0.0 3.4232201652674683 0.0 88 0.0 0.0 0.0 3.66625306815906 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3770 0.0 52.635273 1 GTATCAA 7350 0.0 44.31256 1 TCAACGC 8725 0.0 36.520794 4 ATCAACG 8850 0.0 35.95243 3 CAACGCA 8955 0.0 35.582798 5 TATCAAC 9405 0.0 34.01309 2 AACGCAG 9375 0.0 33.988686 6 ACGCAGA 10810 0.0 29.30287 7 CGCAGAG 10840 0.0 28.961637 8 GTACATG 8145 0.0 26.851158 1 GCAGAGT 12320 0.0 25.101006 9 TACATGG 8220 0.0 24.874422 2 ACATGGG 8405 0.0 23.932547 3 AGAGTAC 11160 0.0 21.414072 10-11 GAGTACT 8055 0.0 20.770977 12-13 CAGAGTA 12105 0.0 20.285885 10-11 AGTACTT 8525 0.0 19.79122 12-13 CATGGGG 6095 0.0 19.0458 4 GTGGTAT 1895 0.0 18.903425 1 GTACTTT 9190 0.0 18.256968 14-15 >>END_MODULE