FastQCFastQC Report
Wed 25 May 2016
SRR1033051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731104
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.037.464731
GTATCAA31550.034.8715551
TCAACGC32000.032.4669464
ATCAACG32250.032.3610343
CAACGCA33250.031.1049965
AACGCAG35250.029.0691816
TATCAAC40650.026.7142282
GTACATG75200.026.3845371
TACATGG77100.024.8159662
ACGCAGA42700.023.6671547
ACATGGG78800.023.505483
CGCAGAG44150.022.7955138
CATGGGG59100.019.7271044
GAGTACA55600.019.280351
AGTACAT55650.018.4154572
GTACCGT2603.6667188E-718.0784876
TACACTG19950.017.6732925
CTAATCG1350.00995034217.4207279
GTATAGA10550.017.380691
GCAGAGT54900.017.2208189
Produced by FastQC (version 0.11.5)