##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033051_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731104 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.002586133666092 31.0 31.0 34.0 28.0 34.0 2 31.041990345296263 33.0 31.0 34.0 28.0 34.0 3 31.223676212989325 33.0 31.0 34.0 28.0 34.0 4 34.64281880148101 37.0 35.0 37.0 32.0 37.0 5 34.652860894348954 37.0 35.0 37.0 32.0 37.0 6 34.82115584027558 37.0 35.0 37.0 32.0 37.0 7 34.65968414238345 37.0 35.0 37.0 32.0 37.0 8 34.669502150046284 37.0 35.0 37.0 32.0 37.0 9 36.28275195671787 39.0 37.0 39.0 32.0 39.0 10-11 36.26797917618663 39.0 37.0 39.0 32.0 39.0 12-13 36.19696997990556 39.0 37.0 39.0 32.0 39.0 14-15 37.49151094209521 40.0 38.0 41.0 32.0 41.0 16-17 37.47000132547131 40.0 38.0 41.0 32.0 41.0 18-19 37.446343310251095 40.0 38.0 41.0 32.0 41.0 20-21 37.48184269072141 40.0 38.0 41.0 32.0 41.0 22-23 37.41379383575287 40.0 38.0 41.0 32.0 41.0 24-25 37.4093923190036 40.0 38.0 41.0 31.5 41.0 26-27 37.31566538659824 40.0 38.0 41.0 31.0 41.0 28-29 37.322062250284134 40.0 38.0 41.0 31.0 41.0 30-31 37.470133323373986 40.0 38.0 41.0 31.0 41.0 32-33 37.465202716557116 40.0 38.0 41.0 31.0 41.0 34-35 37.41893351553071 40.0 38.0 41.0 31.0 41.0 36-37 37.32443619869474 40.0 38.0 41.0 30.5 41.0 38-39 37.221275718537264 40.0 38.0 41.0 30.0 41.0 40-41 37.14198214348483 40.0 38.0 41.0 30.0 41.0 42-43 36.99989399891033 40.0 37.5 41.0 30.0 41.0 44-45 36.82646925931785 40.0 37.0 41.0 30.0 41.0 46-47 36.67389231607437 40.0 37.0 41.0 30.0 41.0 48-49 36.54409114409411 40.0 36.5 41.0 30.0 41.0 50-51 36.40494741320725 40.0 36.0 41.0 30.0 41.0 52-53 36.232445011248195 40.0 36.0 41.0 29.0 41.0 54-55 36.06700715168664 39.5 35.5 41.0 29.0 41.0 56-57 35.88643420389703 39.0 35.0 41.0 28.5 41.0 58-59 35.67755438826204 39.0 35.0 41.0 28.0 41.0 60-61 35.44508649249534 39.0 35.0 41.0 28.0 41.0 62-63 35.187469060130994 38.5 35.0 40.0 28.0 41.0 64-65 34.91017606799302 38.0 34.0 40.0 27.0 41.0 66-67 34.60570468938569 37.5 34.0 40.0 26.5 41.0 68-69 34.289302970520346 37.0 34.0 40.0 26.0 41.0 70-71 33.95117176057741 36.5 34.0 39.0 26.0 41.0 72-73 33.600498736042276 36.0 33.5 39.0 26.0 41.0 74-75 33.24858372291938 35.5 33.0 39.0 26.0 40.0 76-77 32.8875727544612 35.0 33.0 37.5 25.0 40.0 78-79 32.50110999068509 35.0 33.0 37.0 24.5 39.0 80-81 32.14910819946805 35.0 32.5 37.0 24.0 39.0 82-83 31.92896718689585 35.0 33.0 36.0 24.0 38.5 84-85 31.713443171699062 35.0 32.5 36.0 24.0 37.0 86-87 31.541507756570237 35.0 33.0 35.5 24.0 37.0 88-89 31.319609762206053 35.0 32.5 35.0 23.0 36.5 90-91 31.10750579252932 35.0 32.0 35.0 22.0 36.0 92-93 30.87484017452283 35.0 32.0 35.0 20.0 36.0 94-95 30.663763994340748 35.0 32.0 35.0 18.5 36.0 96-97 30.475792390183607 35.0 32.0 35.0 16.5 35.0 98-99 30.28093822132002 35.0 32.0 35.0 2.0 35.0 100 30.11780107970989 34.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023754546557356093 1101 2 0.17444438748633218 1101 3 0.2279911096631082 1101 4 0.1487348438218632 1101 5 0.07914045357321697 1101 6 0.20920338680129902 1101 7 0.25676208085685914 1101 8 0.28393590554181714 1101 9 0.391800852362465 1101 10-11 0.33445191799670226 1101 12-13 0.33884817917111576 1101 14-15 0.4572946366857735 1101 16-17 0.46286951248223573 1101 18-19 0.4258136934289283 1101 20-21 0.44110996975224026 1101 22-23 0.4194902175933706 1101 24-25 0.4252741658101655 1101 26-27 0.32919853001509125 1101 28-29 0.29627854714171775 1101 30-31 0.3491588852238934 1101 32-33 0.3357105516105392 1101 34-35 0.3670230053845742 1101 36-37 0.4547728782496776 1101 38-39 0.5086919535323773 1101 40-41 0.43116026222356396 1101 42-43 0.4594066848956615 1101 44-45 0.46303894522582567 1101 46-47 0.487691826919459 1101 48-49 0.48533341666748697 1101 50-51 0.534078141948541 1101 52-53 0.44829006959201934 1101 54-55 0.327512092062598 1101 56-57 0.4434375165995519 1101 58-59 0.5126487936609934 1101 60-61 0.4260373534104147 1101 62-63 0.39810016577258267 1101 64-65 0.466732443481618 1101 66-67 0.4104538155359734 1101 68-69 0.4411182907760889 1101 70-71 0.38652306335798414 1101 72-73 0.45330730926015406 1101 74-75 0.4647616799215015 1101 76-77 0.4334041172071039 1101 78-79 0.45030330269640473 1101 80-81 0.45737889604615845 1101 82-83 0.4748678012555132 1101 84-85 0.4470439989059365 1101 86-87 0.41484745969086845 1101 88-89 0.4515086491677671 1101 90-91 0.4752217668993062 1101 92-93 0.4680496315422289 1101 94-95 0.4579527839835791 1101 96-97 0.4971752984974884 1101 98-99 0.3832263820858799 1101 100 0.3208941434403769 1102 1 -0.04863125166979998 1102 2 0.024608142609629624 1102 3 0.1428377546636206 1102 4 -0.044317185660815994 1102 5 -0.014460890981318641 1102 6 0.055826739577675255 1102 7 0.14031132346254083 1102 8 0.09550720242835098 1102 9 0.1777740116296016 1102 10-11 0.14794901873572996 1102 12-13 0.1350073262442706 1102 14-15 0.20121630628179332 1102 16-17 0.23856019032343312 1102 18-19 0.17497177316136714 1102 20-21 0.29788718417718485 1102 22-23 0.19117502843824496 1102 24-25 0.20354522275997056 1102 26-27 0.10620964282527012 1102 28-29 0.14568797087598995 1102 30-31 0.1948662011137614 1102 32-33 0.20445078710103104 1102 34-35 0.21016671532091635 1102 36-37 0.1914532658945589 1102 38-39 0.3054486010753479 1102 40-41 0.18516444038962732 1102 42-43 0.28387972379525905 1102 44-45 0.18733345173734506 1102 46-47 0.1045842518095057 1102 48-49 0.09386010571268599 1102 50-51 0.16407765525836737 1102 52-53 0.15769922618404308 1102 54-55 0.20000678913557834 1102 56-57 0.16882412055228002 1102 58-59 0.10412271105938942 1102 60-61 0.1463056885799645 1102 62-63 0.09373545305182773 1102 64-65 0.09498390232389653 1102 66-67 0.1056150021019775 1102 68-69 0.13838970913558057 1102 70-71 0.07319780109799012 1102 72-73 0.11646366546455056 1102 74-75 0.08905862756039085 1102 76-77 0.12824166248310576 1102 78-79 0.09016942298042352 1102 80-81 0.1254682870601087 1102 82-83 0.040715062832596516 1102 84-85 0.03262616326167134 1102 86-87 0.06145592773144415 1102 88-89 0.0023337655742317054 1102 90-91 0.09070701235696887 1102 92-93 0.08453961585760084 1102 94-95 0.10069371557919737 1102 96-97 0.1188239299922671 1102 98-99 0.23035845659584453 1102 100 0.11323219690075703 1103 1 -0.027773006429388403 1103 2 0.04975651728412345 1103 3 0.13029124415610482 1103 4 0.006020122021105578 1103 5 -0.031219800252181074 1103 6 0.05677754173096616 1103 7 0.09902905005241536 1103 8 0.11514516446855083 1103 9 0.21912724372020875 1103 10-11 0.20360038813889503 1103 12-13 0.17617293003971213 1103 14-15 0.2827945716085196 1103 16-17 0.3297084556155596 1103 18-19 0.28939470647691934 1103 20-21 0.24408210222542692 1103 22-23 0.33345605263698985 1103 24-25 0.24105774266097768 1103 26-27 0.22459275507151943 1103 28-29 0.1991406885087983 1103 30-31 0.2020700529629309 1103 32-33 0.17574708073924228 1103 34-35 0.1786460818394957 1103 36-37 0.24518054356699537 1103 38-39 0.305145664924396 1103 40-41 0.27080693038861625 1103 42-43 0.18753266783337352 1103 44-45 0.18532367960819585 1103 46-47 0.19321359435745933 1103 48-49 0.15299411832081233 1103 50-51 0.21953359385584292 1103 52-53 0.2584414510375268 1103 54-55 0.22422710671100532 1103 56-57 0.2924631805381992 1103 58-59 0.252369637845554 1103 60-61 0.23758578685626475 1103 62-63 0.18289198031927612 1103 64-65 0.1528210051899208 1103 66-67 0.1964911364379489 1103 68-69 0.13285780279309023 1103 70-71 0.15444744087595552 1103 72-73 0.24639839451980095 1103 74-75 0.16641639717889944 1103 76-77 0.14181692664041634 1103 78-79 0.13876842701203884 1103 80-81 0.20562877734042218 1103 82-83 0.1186592352810294 1103 84-85 0.12280629157763556 1103 86-87 0.13629146221923705 1103 88-89 0.20606051694524652 1103 90-91 0.1740652069914752 1103 92-93 0.17233677649458556 1103 94-95 0.14071073094079622 1103 96-97 0.2862082068000191 1103 98-99 0.1918217467776131 1103 100 0.15125913043095096 1104 1 -0.059752893312190025 1104 2 -0.012137210186278224 1104 3 0.01618298047065636 1104 4 0.12227605495479565 1104 5 -0.015071642931992812 1104 6 0.06890556039208207 1104 7 0.11292383084759194 1104 8 0.18394470738001445 1104 9 0.22713004111093937 1104 10-11 0.1986053896269837 1104 12-13 0.2077905456611191 1104 14-15 0.2782866733384779 1104 16-17 0.2305612072530181 1104 18-19 0.2958088060924595 1104 20-21 0.2780593916104479 1104 22-23 0.2645332771123279 1104 24-25 0.10666499522127282 1104 26-27 0.12308090502899205 1104 28-29 0.13368369575380967 1104 30-31 0.1981019697586035 1104 32-33 0.16184181944583997 1104 34-35 0.12884584291190748 1104 36-37 0.18924747799766806 1104 38-39 0.1647549144707483 1104 40-41 0.17829213692245105 1104 42-43 0.16245988042617654 1104 44-45 0.22941499681386546 1104 46-47 0.23276106032568578 1104 48-49 0.17680292987950708 1104 50-51 0.1625988772263156 1104 52-53 0.08209769846945392 1104 54-55 0.1414927697114905 1104 56-57 0.17696302340012693 1104 58-59 0.14216147294054338 1104 60-61 0.18400996579598683 1104 62-63 0.17113202842684672 1104 64-65 0.11619228856926611 1104 66-67 0.22561637158978698 1104 68-69 0.2993212565323091 1104 70-71 0.1905362383199929 1104 72-73 0.23865653377188778 1104 74-75 0.33387660876558556 1104 76-77 0.2943924014972126 1104 78-79 0.2581684400050861 1104 80-81 0.23404730763864023 1104 82-83 0.25714506842372487 1104 84-85 0.24144521911866335 1104 86-87 0.23069968609267733 1104 88-89 0.28027931032479003 1104 90-91 0.3378360749821745 1104 92-93 0.308721304447392 1104 94-95 0.3217003287339786 1104 96-97 0.3405419552813882 1104 98-99 0.41265694948016574 1104 100 0.2962350988567479 1105 1 -0.06901958320686319 1105 2 -0.009595391983687307 1105 3 0.0014136153741475255 1105 4 -0.08848449267230762 1105 5 -0.09594600087316252 1105 6 1.9338811988234283E-4 1105 7 0.0044389962095436886 1105 8 -0.006543215786862788 1105 9 0.09167600971885292 1105 10-11 0.13238814033888247 1105 12-13 0.2013094317448818 1105 14-15 0.20404290985082696 1105 16-17 0.13809117086363187 1105 18-19 0.1882120597962995 1105 20-21 0.19830201611222265 1105 22-23 0.17964407185289843 1105 24-25 0.11009597272922633 1105 26-27 0.07501791446281203 1105 28-29 0.11888080625542585 1105 30-31 0.12771442118229714 1105 32-33 0.07969392367486705 1105 34-35 0.07070398270158762 1105 36-37 0.170390894415263 1105 38-39 0.3007068140613711 1105 40-41 0.3216690586852593 1105 42-43 0.3124532144899348 1105 44-45 0.17724512097812095 1105 46-47 0.06627975688135734 1105 48-49 0.041989042559926304 1105 50-51 0.09114945214545855 1105 52-53 0.1890986320899799 1105 54-55 0.15866333226198748 1105 56-57 0.140209569315779 1105 58-59 0.17143178074452692 1105 60-61 0.13291131305503967 1105 62-63 0.024222192906073303 1105 64-65 0.1358466101210425 1105 66-67 0.12116173474635872 1105 68-69 0.14249115132085421 1105 70-71 0.039008343421365055 1105 72-73 0.10078053472523862 1105 74-75 0.09558298589219305 1105 76-77 0.14117903727512982 1105 78-79 0.07539723051475988 1105 80-81 0.19073923260005898 1105 82-83 0.16846046599651743 1105 84-85 0.20165558360452707 1105 86-87 0.10291599241367066 1105 88-89 0.1403909060162185 1105 90-91 0.1014300089931659 1105 92-93 0.11634725234742405 1105 94-95 0.19907553098380504 1105 96-97 0.286007002668601 1105 98-99 0.26866818304314677 1105 100 0.3093382395187767 1106 1 0.12464408784919101 1106 2 0.11274018195915403 1106 3 0.13737116846146336 1106 4 0.12693036258162493 1106 5 0.20097020486230122 1106 6 0.2270876584602135 1106 7 0.3252178496519207 1106 8 0.31290144250368 1106 9 0.3398940924123295 1106 10-11 0.3281858488725078 1106 12-13 0.3371309582690145 1106 14-15 0.38545744256691705 1106 16-17 0.47360521294555724 1106 18-19 0.4355436541570512 1106 20-21 0.39066906417875913 1106 22-23 0.3714175410970597 1106 24-25 0.366244419391748 1106 26-27 0.38403867472340636 1106 28-29 0.34363180710115415 1106 30-31 0.43337963462928286 1106 32-33 0.38961172314478176 1106 34-35 0.36345458580380097 1106 36-37 0.30449403892614413 1106 38-39 0.3389234693193828 1106 40-41 0.4009995969768738 1106 42-43 0.3503698445301353 1106 44-45 0.35371181397700013 1106 46-47 0.3619197359440065 1106 48-49 0.35090113492573494 1106 50-51 0.39536462549605744 1106 52-53 0.36987667704075733 1106 54-55 0.34062611183644975 1106 56-57 0.34824871772998733 1106 58-59 0.3492447840006392 1106 60-61 0.3071423550467358 1106 62-63 0.33416971272315976 1106 64-65 0.30600412428054113 1106 66-67 0.33133396983240715 1106 68-69 0.3646264549036289 1106 70-71 0.32434023872747275 1106 72-73 0.3058051995462847 1106 74-75 0.30162295788820614 1106 76-77 0.2890703065658329 1106 78-79 0.2745560986829716 1106 80-81 0.3339365886485339 1106 82-83 0.3024937109499035 1106 84-85 0.23971802430569156 1106 86-87 0.28089701224545394 1106 88-89 0.3293918563925651 1106 90-91 0.33510683674697717 1106 92-93 0.3160615750949347 1106 94-95 0.273188844822144 1106 96-97 0.2126565417455808 1106 98-99 0.14250248753764083 1106 100 0.17403698776154997 1107 1 -0.148944304778162 1107 2 -0.15046080828224717 1107 3 -0.09495301252853139 1107 4 -0.132772446278004 1107 5 -0.07091808372058495 1107 6 -0.08939687407229968 1107 7 -0.035062390404398514 1107 8 -0.04561427915366778 1107 9 -0.028798039356637162 1107 10-11 -0.04107258263442759 1107 12-13 -0.061202091096539846 1107 14-15 -0.029128476866588926 1107 16-17 -0.16935947887848357 1107 18-19 -0.10861185690531983 1107 20-21 -0.10590275215498224 1107 22-23 -0.1823872814105414 1107 24-25 -0.194589648937189 1107 26-27 -0.08789861813757938 1107 28-29 -0.10326605106866538 1107 30-31 -0.028337756676336312 1107 32-33 -0.09941168295111424 1107 34-35 -0.10074352218909866 1107 36-37 0.009672241884366883 1107 38-39 0.008950829551551465 1107 40-41 -0.07315070510352939 1107 42-43 -0.08753753879481252 1107 44-45 -0.16747765820202432 1107 46-47 -0.10005448996854227 1107 48-49 -0.09609240442029687 1107 50-51 -0.08656911693503844 1107 52-53 -0.17211525224027469 1107 54-55 -0.16741040169899435 1107 56-57 -0.1754688415466532 1107 58-59 -0.12054220960101247 1107 60-61 -0.15119722038625838 1107 62-63 -0.19813540843548338 1107 64-65 -0.19097339669659164 1107 66-67 -0.15468100380149252 1107 68-69 -0.13795881756527706 1107 70-71 -0.11254876893355004 1107 72-73 -0.15278218895767282 1107 74-75 -0.20387306761094948 1107 76-77 -0.20854480485366622 1107 78-79 -0.2355705901294627 1107 80-81 -0.19689714056469043 1107 82-83 -0.15959041901639992 1107 84-85 -0.2618588931630086 1107 86-87 -0.32681266309032964 1107 88-89 -0.30645246328106523 1107 90-91 -0.21657766033412784 1107 92-93 -0.1839891453320135 1107 94-95 -0.2229192880719566 1107 96-97 -0.16899169111184165 1107 98-99 -0.1736294998256227 1107 100 -0.2478764465162051 1108 1 -0.07876062527095229 1108 2 -0.016304054354293385 1108 3 0.013027893991996109 1108 4 0.09576267845313424 1108 5 0.025222938306825426 1108 6 0.0320749179792017 1108 7 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-0.18372182500945655 2214 100 -0.20636449143476554 2215 1 -0.11954620935550508 2215 2 -0.10418457780182777 2215 3 -0.18240005504419798 2215 4 -0.17083203247147338 2215 5 -0.10797642788222817 2215 6 -0.09942284032268134 2215 7 -0.13583236336314997 2215 8 -0.18072926411213786 2215 9 -0.24372604224515015 2215 10-11 -0.24133788525947608 2215 12-13 -0.29902621428063725 2215 14-15 -0.405387832779212 2215 16-17 -0.4353489694526118 2215 18-19 -0.28506015687877806 2215 20-21 -0.34647862781393 2215 22-23 -0.32605987285582216 2215 24-25 -0.2871025326371637 2215 26-27 -0.3194223161674117 2215 28-29 -0.3980891669849669 2215 30-31 -0.3679433627640023 2215 32-33 -0.286557147703121 2215 34-35 -0.33208251830054536 2215 36-37 -0.31486332185176735 2215 38-39 -0.3424078009306015 2215 40-41 -0.2780840998515117 2215 42-43 -0.2591116652930836 2215 44-45 -0.207838630780671 2215 46-47 -0.2298574207587052 2215 48-49 -0.1484375740541779 2215 50-51 -0.22890538530067772 2215 52-53 -0.31122030962127667 2215 54-55 -0.2898877068394441 2215 56-57 -0.42073141690794813 2215 58-59 -0.3684829266243099 2215 60-61 -0.4585907444183661 2215 62-63 -0.3885012618882584 2215 64-65 -0.6153528929759133 2215 66-67 -0.4735077525343385 2215 68-69 -0.4445166154305582 2215 70-71 -0.38677531910519747 2215 72-73 -0.4959181493823479 2215 74-75 -0.4088619717570836 2215 76-77 -0.3603748916223566 2215 78-79 -0.37129837184809134 2215 80-81 -0.4190918852732821 2215 82-83 -0.381510942959558 2215 84-85 -0.37605112536632035 2215 86-87 -0.3061536703629528 2215 88-89 -0.3257561155969988 2215 90-91 -0.3343227832562299 2215 92-93 -0.36048338578002515 2215 94-95 -0.42498323499503954 2215 96-97 -0.3217229439153293 2215 98-99 -0.2048939692071201 2215 100 -0.22054842019949916 2216 1 -0.41049880920993687 2216 2 -0.39992624292785095 2216 3 -0.3691428710715421 2216 4 -0.4746861361591854 2216 5 -0.38889161927995275 2216 6 -0.5789355893445816 2216 7 -0.5102214779352892 2216 8 -0.4266217039729696 2216 9 -0.5265853574671908 2216 10-11 -0.47733738309341334 2216 12-13 -0.6631088076893548 2216 14-15 -0.7374023556215192 2216 16-17 -0.7696248634830809 2216 18-19 -0.7466550712607116 2216 20-21 -0.6215401910410563 2216 22-23 -0.5750498986553367 2216 24-25 -0.6591737927491863 2216 26-27 -0.5962412732276334 2216 28-29 -0.6823531522680852 2216 30-31 -0.8538323923926328 2216 32-33 -0.8830124007931488 2216 34-35 -0.9896818605712738 2216 36-37 -0.9806512187336764 2216 38-39 -0.9096117144947442 2216 40-41 -0.7514210391202312 2216 42-43 -0.800932958299903 2216 44-45 -0.7556275802959078 2216 46-47 -0.733574532212927 2216 48-49 -0.722588451220858 2216 50-51 -0.7284311381750186 2216 52-53 -0.7683418238143389 2216 54-55 -0.8149853710594002 2216 56-57 -0.7919614447727525 2216 58-59 -0.7437477296969348 2216 60-61 -0.64820107021837 2216 62-63 -0.8116308598722668 2216 64-65 -0.8112865346042355 2216 66-67 -0.9072871225865882 2216 68-69 -0.8311549384457564 2216 70-71 -0.884253454478511 2216 72-73 -0.9953764054753691 2216 74-75 -1.008539311326274 2216 76-77 -0.85816733881164 2216 78-79 -0.8456078935161848 2216 80-81 -0.7827153881438704 2216 82-83 -0.8710495039831443 2216 84-85 -0.7642905294959057 2216 86-87 -0.8128986641087792 2216 88-89 -0.9645687218804859 2216 90-91 -0.9497628905848572 2216 92-93 -1.0794725288332288 2216 94-95 -1.0114582499434306 2216 96-97 -1.0426189439086677 2216 98-99 -0.8738120553465052 2216 100 -0.7902220455719586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50366.0 3 8832.0 4 5301.0 5 4074.0 6 3562.0 7 3661.0 8 4299.0 9 4851.0 10 5514.0 11 5832.0 12 6680.0 13 7167.0 14 7540.0 15 7978.0 16 7864.0 17 7799.0 18 7791.0 19 7650.0 20 7634.0 21 7975.0 22 9084.0 23 12172.0 24 17407.0 25 21853.0 26 22745.0 27 21712.0 28 25059.0 29 30470.0 30 36986.0 31 45541.0 32 58153.0 33 76859.0 34 109548.0 35 166431.0 36 293519.0 37 561570.0 38 781947.0 39 267293.0 40 385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40064643438896 19.24442943115679 13.233183143464908 24.12174099098934 2 15.392499356222153 19.748283778007085 40.66767546563545 24.191541400135314 3 18.46437009337988 24.778548382310543 29.510225479675984 27.2468560446336 4 12.093997081828261 16.304203647641387 38.929451622619275 32.672347647911074 5 12.839487171030331 37.98939821202477 35.72875840346528 13.442356213479625 6 31.482183882140347 37.925641056650015 17.6064237777404 12.985751283469238 7 27.83736488455062 32.17113737936039 22.05388309753599 17.937614638553004 8 25.1750209922566 36.92082538144332 19.94291865486824 17.96123497143184 9 24.97334628788648 17.064019852571715 20.7678687988659 37.194765060675905 10-11 24.028779667968355 26.908560725220365 28.685161493917004 20.37749811289428 12-13 24.623978955173634 24.097730009641634 28.80725030481754 22.471040730367196 14-15 21.854493852496947 26.239328441623964 25.622039852702578 26.28413785317651 16-17 21.7091573043383 29.62537983980864 26.106120271399735 22.559342584453326 18-19 21.604006295754214 28.58324822874647 28.00815318129284 21.804592294206483 20-21 22.787622254288923 27.909571909571913 27.588373531230676 21.714432304908495 22-23 22.471974739072632 27.660902311005568 27.620035382590284 22.247087567331516 24-25 22.293800156564014 27.660576540118388 27.60214428610014 22.443479017217463 26-27 22.046275211212222 27.6194460502801 27.748381611111274 22.585897127396404 28-29 22.08323669686854 27.767219518843834 27.70914604142233 22.440397742865297 30-31 22.32009648915001 28.203508852930813 27.31884786854141 22.157546789377765 32-33 21.88263012724856 27.893074933838257 27.973375911355635 22.250919027557543 34-35 22.07665122411236 27.543919723132703 27.98615663511014 22.3932724176448 36-37 22.384560631243534 28.029877024152267 27.400912107199154 22.18465023740504 38-39 22.24346875294285 28.11707491884607 27.530465473074557 22.108990855136522 40-41 21.90369363602018 27.79902943893538 27.869679659149334 22.42759726589511 42-43 21.995404696179918 27.634682690075685 27.844532821465613 22.525379792278784 44-45 22.13244964926285 27.85733666204929 27.8102162902014 22.199997398486467 46-47 22.11947177643353 28.087069383758717 27.639762022370128 22.15369681743762 48-49 22.009670275874566 27.863299945457896 27.720156704565213 22.406873074102325 50-51 22.073190191500856 27.773404301509835 27.726101140460525 22.42730436652878 52-53 22.11955106836444 27.70257903562766 27.790098682661675 22.387771213346223 54-55 22.229397354812566 28.14131579352325 27.556759268011906 22.072527583652278 56-57 22.014508187396228 27.85837983663496 27.638835278011303 22.488276697957506 58-59 21.91545808406928 27.446793543413712 28.076499761116857 22.56124861140015 60-61 22.2344897071283 27.935004923175562 27.51773946096952 22.312765908726615 62-63 21.99471946904503 27.915897688430352 27.64961779171068 22.439765050813936 64-65 22.033586877848528 27.598846546794046 27.83104303026761 22.53652354508981 66-67 21.88750539511278 27.765558625915155 27.63673542366932 22.710200555302745 68-69 22.393020722615052 27.908609306948907 27.50759616712777 22.190773803308275 70-71 22.256910651313877 27.60272669004426 27.647807603964996 22.492555054676867 72-73 21.99265339872357 27.713444082501898 27.87466045370291 22.419242065071625 74-75 22.143972724844726 27.80435146584542 27.590357561429368 22.461318247880484 76-77 22.076145774985083 27.777590534202258 27.514590650333982 22.631673040478685 78-79 22.16401610030177 27.901493261776437 27.585000526602588 22.34949011131921 80-81 22.06529543206711 27.667991501520202 27.726345287373167 22.540367779039524 82-83 21.936075448086577 27.73447660161233 27.90035454211754 22.429093408183558 84-85 21.999850915395953 27.655853393057527 27.685047601761685 22.65924808978483 86-87 22.162618049685946 27.818252246526846 27.624262947805857 22.39486675598135 88-89 21.855107586159402 27.665423198112233 27.740899443882377 22.738569771845988 90-91 21.874361856300155 27.597660367171517 27.849520518855353 22.67845725767297 92-93 22.079245747457705 27.827036784426745 27.64242234655207 22.45129512156348 94-95 21.839665266326527 27.50849709401033 27.825450879855357 22.826386759807786 96-97 21.82137532646879 27.543104869284278 27.91054480452166 22.724974999725273 98-99 21.90125042308374 27.59260588815351 27.773341401839442 22.732802286923306 100 22.00180236945643 27.45125909427271 27.72857488258955 22.818363653681303 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 219.0 1 218.5 2 201.5 3 205.5 4 240.5 5 253.5 6 253.5 7 248.0 8 222.5 9 217.0 10 204.0 11 189.0 12 193.0 13 195.5 14 207.5 15 247.0 16 321.0 17 429.5 18 609.5 19 849.0 20 1220.5 21 1864.0 22 2871.5 23 4496.5 24 6570.5 25 9028.5 26 12389.5 27 17110.0 28 23449.5 29 30454.5 30 37296.5 31 44733.0 32 52759.5 33 61398.5 34 70541.5 35 78407.5 36 85169.0 37 91759.5 38 96797.5 39 99811.5 40 102111.5 41 103135.5 42 102184.0 43 100890.0 44 99343.5 45 97416.0 46 96428.5 47 93961.0 48 91220.0 49 89108.5 50 86134.0 51 83588.5 52 79571.0 53 75791.0 54 72860.5 55 68632.5 56 63707.5 57 58628.0 58 53463.0 59 48707.0 60 43595.5 61 37471.0 62 31863.5 63 26629.5 64 21473.0 65 17248.0 66 13531.0 67 10008.0 68 7486.0 69 5813.0 70 4291.0 71 3084.0 72 2328.0 73 1708.5 74 1258.5 75 888.0 76 580.0 77 407.0 78 264.5 79 154.0 80 105.5 81 67.0 82 42.5 83 30.5 84 30.0 85 26.0 86 16.5 87 12.5 88 9.0 89 7.5 90 6.0 91 6.0 92 3.0 93 3.0 94 5.0 95 3.0 96 1.5 97 1.5 98 2.0 99 3.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06114743341886651 2 0.04119213329115259 3 0.05495945961779559 4 0.073157228725087 5 0.08491071742416254 6 0.08666824844458504 7 0.08857224038337609 8 0.09948357880183252 9 0.09487005987322343 10-11 0.09957511687581286 12-13 0.1037126378197242 14-15 0.10554339929933097 16-17 0.07297415257712632 18-19 0.09514467409516444 20-21 0.08848070230939575 22-23 0.055398842372901214 24-25 0.021657908303748228 26-27 0.1807327732667815 28-29 0.06601725895462054 30-31 0.0766173679215438 32-33 0.0731023058806988 34-35 0.020284837194043143 36-37 0.05056563206673931 38-39 0.07436553130162747 40-41 0.07934520252615793 42-43 0.0737247647837651 44-45 0.07042939412047289 46-47 0.07718490398022192 48-49 0.07394445616131791 50-51 0.06955062861026164 52-53 0.0733219972582516 54-55 0.06480895637808007 56-57 0.06654817978370652 58-59 0.06356403857194746 60-61 0.06101928011529404 62-63 0.06711571584238463 64-65 0.06350911572755925 66-67 0.0652483391331857 68-69 0.06469911068930367 70-71 0.05739437238567261 72-73 0.05647899164586921 74-75 0.05810836936271925 76-77 0.05290900676063599 78-79 0.04911933049784995 80-81 0.044084736428931307 82-83 0.050748708214699983 84-85 0.04066121246206662 86-87 0.038683990064091296 88-89 0.04049644392890201 90-91 0.03725599610999801 92-93 0.03991060025542784 94-95 0.038555836760518825 96-97 0.041521670357481805 98-99 0.042034283571771705 100 0.049943173163673006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2731104.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.33318970250384 #Duplication Level Percentage of deduplicated Percentage of total 1 77.95251347898642 51.70838864388595 2 13.42945492974149 17.81637162911536 3 4.17362514797145 8.305496060625925 4 1.6718816642227246 4.436049743720953 5 0.8474696478843488 2.8107682460113326 6 0.5121237995662076 2.038248308867538 7 0.31410709236381035 1.4585007741269371 8 0.23016219631213486 1.2213914104254218 9 0.15011997099224503 0.8962142862566667 >10 0.6894649539092632 7.695087738956455 >50 0.02390538252370983 1.0697851207278297 >100 0.005116499529541501 0.5237293471254983 >500 5.5235996659105976E-5 0.019968690154134633 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.323045918427127E-5 0.0 0.0 0.0 6 0.0 7.323045918427127E-5 0.0 0.0 0.0 7 0.0 7.323045918427127E-5 0.0 0.0 0.0 8 0.0 7.323045918427127E-5 0.0 3.6615229592135635E-5 0.0 9 0.0 7.323045918427127E-5 0.0 3.6615229592135635E-5 0.0 10-11 0.0 7.323045918427127E-5 0.0 3.6615229592135635E-5 0.0 12-13 0.0 9.153807398033908E-5 0.0 3.6615229592135635E-5 0.0 14-15 0.0 1.098456887764069E-4 0.0 3.6615229592135635E-5 0.0 16-17 0.0 1.098456887764069E-4 0.0 3.6615229592135635E-5 0.0 18-19 0.0 1.098456887764069E-4 0.0 3.6615229592135635E-5 0.0 20-21 0.0 1.4646091836854254E-4 0.0 7.323045918427127E-5 0.0 22-23 0.0 1.8307614796067817E-4 0.0 1.281533035724747E-4 0.0 24-25 0.0 1.8307614796067817E-4 0.0 1.6476853316461037E-4 0.0 26-27 0.0 1.8307614796067817E-4 0.0 2.3799899234888162E-4 0.0 28-29 0.0 1.8307614796067817E-4 0.0 4.7599798469776325E-4 0.0 30-31 0.0 1.8307614796067817E-4 0.0 0.0019406071683831885 0.0 32-33 3.6615229592135635E-5 1.8307614796067817E-4 0.0 0.0063710499490316 0.0 34-35 7.323045918427127E-5 2.0138376275674597E-4 0.0 0.011954872461832284 0.0 36-37 7.323045918427127E-5 2.196913775528138E-4 0.0 0.02348866978335501 0.0 38-39 7.323045918427127E-5 2.196913775528138E-4 0.0 0.044176274502911644 0.0 40-41 7.323045918427127E-5 2.196913775528138E-4 0.0 0.07205877183732293 0.0 42-43 7.323045918427127E-5 2.196913775528138E-4 0.0 0.10175372303654492 0.0 44-45 7.323045918427127E-5 2.3799899234888162E-4 0.0 0.13245559304955065 0.0 46-47 7.323045918427127E-5 2.563066071449494E-4 0.0 0.17113958311364197 0.0 48-49 7.323045918427127E-5 2.563066071449494E-4 0.0 0.21676215918544295 0.0 50-51 1.4646091836854254E-4 2.563066071449494E-4 0.0 0.2685910166731109 0.0 52-53 1.4646091836854254E-4 2.746142219410173E-4 0.0 0.31976080002812046 0.0 54-55 1.8307614796067817E-4 2.929218367370851E-4 0.0 0.37574548607449587 0.0 56-57 1.8307614796067817E-4 2.929218367370851E-4 0.0 0.4457538050546592 0.0 58-59 1.8307614796067817E-4 3.112294515331529E-4 0.0 0.5227007100425323 0.0 60-61 1.8307614796067817E-4 3.2953706632922073E-4 0.0 0.6072818904003656 0.0 62-63 1.8307614796067817E-4 3.2953706632922073E-4 0.0 0.6973553551970193 0.0 64-65 1.8307614796067817E-4 3.4784468112528853E-4 0.0 0.7905960373533927 0.0 66-67 1.8307614796067817E-4 3.6615229592135633E-4 0.0 0.8961760518823157 0.0 68-69 1.8307614796067817E-4 3.6615229592135633E-4 0.0 1.0204847563476163 0.0 70-71 1.8307614796067817E-4 3.6615229592135633E-4 0.0 1.1552471088614715 0.0 72-73 1.8307614796067817E-4 4.02767525513492E-4 0.0 1.3019277186075668 0.0 74-75 1.8307614796067817E-4 4.02767525513492E-4 0.0 1.4504573974480648 0.0 76-77 2.3799899234888162E-4 4.02767525513492E-4 0.0 1.6071339648728133 0.0 78-79 2.563066071449494E-4 4.02767525513492E-4 0.0 1.7939265586370934 0.0 80-81 2.563066071449494E-4 4.02767525513492E-4 0.0 2.00312767291176 0.0 82-83 2.563066071449494E-4 4.02767525513492E-4 0.0 2.2223430524798764 0.0 84-85 2.563066071449494E-4 4.02767525513492E-4 0.0 2.4399290543311425 0.0 86-87 2.563066071449494E-4 4.02767525513492E-4 0.0 2.6693966981850563 0.0 88 2.563066071449494E-4 4.02767525513492E-4 0.0 2.863640491171336 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1895 0.0 37.46473 1 GTATCAA 3155 0.0 34.871555 1 TCAACGC 3200 0.0 32.466946 4 ATCAACG 3225 0.0 32.361034 3 CAACGCA 3325 0.0 31.104996 5 AACGCAG 3525 0.0 29.069181 6 TATCAAC 4065 0.0 26.714228 2 GTACATG 7520 0.0 26.384537 1 TACATGG 7710 0.0 24.815966 2 ACGCAGA 4270 0.0 23.667154 7 ACATGGG 7880 0.0 23.50548 3 CGCAGAG 4415 0.0 22.795513 8 CATGGGG 5910 0.0 19.727104 4 GAGTACA 5560 0.0 19.28035 1 AGTACAT 5565 0.0 18.415457 2 GTACCGT 260 3.6667188E-7 18.078487 6 TACACTG 1995 0.0 17.673292 5 CTAATCG 135 0.009950342 17.420727 9 GTATAGA 1055 0.0 17.38069 1 GCAGAGT 5490 0.0 17.220818 9 >>END_MODULE