FastQCFastQC Report
Wed 25 May 2016
SRR1033051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731104
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.12460162630203757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.1080881577559844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.053.6783451
GTATCAA63050.042.53021
TCAACGC74250.035.3187834
ATCAACG74550.035.241633
CAACGCA75150.034.8964425
AACGCAG78550.033.505636
TATCAAC79700.033.2179532
ACGCAGA91950.028.5716957
CGCAGAG93200.027.9368698
GTACATG74200.026.8027151
TACATGG77300.024.9861432
GCAGAGT108900.023.6934539
GTGGTAT16000.023.268981
ACATGGG79350.022.8037173
GAGTACT70700.020.6736412-13
CAGAGTA104350.020.26767710-11
TGGTATC17150.020.0518112
AGAGTAC100200.019.69996610-11
TATACCG1456.6053256E-419.4472965
AGTACTT73200.019.10081312-13
Produced by FastQC (version 0.11.5)