FastQCFastQC Report
Wed 25 May 2016
SRR1033050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437371
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.034.9755251
GTATCAA29800.033.2835541
ATCAACG29200.033.0015643
TCAACGC29950.032.33214
CAACGCA30400.031.6995245
AACGCAG34300.028.638116
GTACATG65700.027.9037631
TATCAAC37050.026.516832
TACATGG67000.026.1696282
ACATGGG70700.024.0687033
CGCAGAG40650.023.612118
ACGCAGA40650.023.5883457
CATGGGG50150.021.9335274
GCAGAGT49850.018.5031419
GTCGTAC1350.00998524717.410041
GAGTACA49800.017.1793061
AGAGTAC41900.016.40551610-11
TCTATAC8750.016.1167223
TACGCCC2355.6177276E-516.002424
GTATTAG11550.015.8725291
Produced by FastQC (version 0.11.5)