##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033050_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2437371
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136671848479367	31.0	31.0	34.0	28.0	34.0
2	31.284726042937248	33.0	31.0	34.0	28.0	34.0
3	31.518276454425692	34.0	31.0	34.0	28.0	34.0
4	34.90615011009813	37.0	35.0	37.0	32.0	37.0
5	35.03226304079272	37.0	35.0	37.0	33.0	37.0
6	35.06908878459619	37.0	35.0	37.0	32.0	37.0
7	34.94179753513109	37.0	35.0	37.0	32.0	37.0
8	34.97997391451691	37.0	35.0	37.0	32.0	37.0
9	36.58134317672607	39.0	37.0	39.0	33.0	39.0
10-11	36.57895720429922	39.0	37.0	39.0	33.0	39.0
12-13	36.52125733013152	39.0	37.0	39.0	33.0	39.0
14-15	37.86683767058852	40.0	38.0	41.0	33.0	41.0
16-17	37.86311316578396	40.0	38.0	41.0	33.0	41.0
18-19	37.84442479212233	40.0	38.0	41.0	33.0	41.0
20-21	37.859588671564566	40.0	38.0	41.0	33.0	41.0
22-23	37.820902111332245	40.0	38.0	41.0	32.0	41.0
24-25	37.81818360848636	40.0	38.0	41.0	32.0	41.0
26-27	37.72039402290419	40.0	38.0	41.0	32.0	41.0
28-29	37.7344546644725	40.0	38.0	41.0	32.0	41.0
30-31	37.88108991204047	40.0	38.0	41.0	32.5	41.0
32-33	37.864964956094084	40.0	38.0	41.0	32.5	41.0
34-35	37.82803746331601	40.0	38.0	41.0	32.0	41.0
36-37	37.747937224164886	40.0	38.0	41.0	32.0	41.0
38-39	37.653058561868505	40.0	38.0	41.0	32.0	41.0
40-41	37.57727608968844	40.0	38.0	41.0	32.0	41.0
42-43	37.458392259528814	40.0	38.0	41.0	31.0	41.0
44-45	37.31278845116316	40.0	38.0	41.0	31.0	41.0
46-47	37.17763299062801	40.0	37.5	41.0	31.0	41.0
48-49	37.06291881703688	40.0	37.0	41.0	30.0	41.0
50-51	36.932357035510805	40.0	37.0	41.0	30.0	41.0
52-53	36.77099218789425	40.0	36.5	41.0	30.0	41.0
54-55	36.61073180898599	40.0	36.0	41.0	30.0	41.0
56-57	36.43609097671221	40.0	36.0	41.0	30.0	41.0
58-59	36.231837910601215	39.0	35.0	41.0	30.0	41.0
60-61	36.00681082198812	39.0	35.0	41.0	29.0	41.0
62-63	35.75660886258186	39.0	35.0	41.0	29.0	41.0
64-65	35.48159348740918	38.5	35.0	40.0	28.5	41.0
66-67	35.18296845248425	38.0	35.0	40.0	28.0	41.0
68-69	34.86602203767912	37.0	34.0	40.0	28.0	41.0
70-71	34.534514647134145	37.0	34.0	39.5	28.0	41.0
72-73	34.18483767961463	36.0	34.0	39.0	27.5	41.0
74-75	33.83051390206907	36.0	34.0	39.0	27.0	40.5
76-77	33.46036262021662	35.0	34.0	38.5	26.5	40.0
78-79	33.071963193128994	35.0	33.0	37.0	26.0	39.0
80-81	32.718829222141395	35.0	33.0	37.0	26.0	39.0
82-83	32.49370428219586	35.0	33.0	36.5	26.0	39.0
84-85	32.26911065242017	35.0	33.0	36.0	26.0	37.0
86-87	32.09265167264237	35.0	33.0	36.0	26.0	37.0
88-89	31.86898547656471	35.0	33.0	35.0	25.5	37.0
90-91	31.650257798258863	35.0	33.0	35.0	25.0	36.0
92-93	31.42622973687633	35.0	33.0	35.0	25.0	36.0
94-95	31.220550954286402	35.0	33.0	35.0	24.0	36.0
96-97	31.04101837594687	35.0	33.0	35.0	23.5	35.5
98-99	30.859766116852953	35.0	32.0	35.0	21.5	35.0
100	30.712195640302603	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12539805537754845
1101	2	0.2132959320500376
1101	3	0.24459797419519447
1101	4	0.10644909912955569
1101	5	0.1367035228657727
1101	6	0.23494310124327455
1101	7	0.32731908086346806
1101	8	0.34195903491362145
1101	9	0.45696558461483505
1101	10-11	0.4166983257924457
1101	12-13	0.3692151921792046
1101	14-15	0.4891657722092191
1101	16-17	0.5295071237602258
1101	18-19	0.4910875934610104
1101	20-21	0.4749435384314964
1101	22-23	0.4565149012956695
1101	24-25	0.5275731012787972
1101	26-27	0.39407589241590557
1101	28-29	0.3678097648651715
1101	30-31	0.4506978507355086
1101	32-33	0.41045067880341435
1101	34-35	0.38564931039238814
1101	36-37	0.5027369800317842
1101	38-39	0.5309168272813736
1101	40-41	0.5381629863297306
1101	42-43	0.5125752659990539
1101	44-45	0.4514114933208191
1101	46-47	0.4783248630229622
1101	48-49	0.5449310233243594
1101	50-51	0.46732585144462746
1101	52-53	0.46759718325728983
1101	54-55	0.40571311640428576
1101	56-57	0.5007495266968505
1101	58-59	0.49543697221496075
1101	60-61	0.5308871999219349
1101	62-63	0.5448108570825738
1101	64-65	0.49886678686141295
1101	66-67	0.44560038010373404
1101	68-69	0.4674296746726867
1101	70-71	0.29906043142043615
1101	72-73	0.2941564978327591
1101	74-75	0.3174357560075407
1101	76-77	0.2805268859082517
1101	78-79	0.26941292244247705
1101	80-81	0.3572013229368878
1101	82-83	0.3380846578465224
1101	84-85	0.4132062429015235
1101	86-87	0.4017744477250247
1101	88-89	0.43082928749014116
1101	90-91	0.3412895159662277
1101	92-93	0.35354204935973854
1101	94-95	0.4618755898164224
1101	96-97	0.4477246389253686
1101	98-99	0.5187683294915217
1101	100	0.5752549455658773
1102	1	0.07269520464664225
1102	2	0.16192854952911162
1102	3	0.1582491363816878
1102	4	0.04859422884420184
1102	5	0.16752725264810664
1102	6	0.2638599597234972
1102	7	0.2791571731390121
1102	8	0.32968492784725356
1102	9	0.4278648414221635
1102	10-11	0.3236241789121763
1102	12-13	0.3508817985749886
1102	14-15	0.441286406198067
1102	16-17	0.4356467929327579
1102	18-19	0.4081080589956585
1102	20-21	0.42485685368590254
1102	22-23	0.43935248905125235
1102	24-25	0.4064506549503193
1102	26-27	0.21944150262174844
1102	28-29	0.3171154267237384
1102	30-31	0.35587482419150973
1102	32-33	0.3442823762796223
1102	34-35	0.33669998989687144
1102	36-37	0.37431575887579527
1102	38-39	0.40138062402407826
1102	40-41	0.34256494935827675
1102	42-43	0.3641789425698079
1102	44-45	0.39234280838704194
1102	46-47	0.35134922147158676
1102	48-49	0.2785683998626709
1102	50-51	0.34872214292445136
1102	52-53	0.3793594237472391
1102	54-55	0.39243975611172743
1102	56-57	0.3201020190325039
1102	58-59	0.32692287128568864
1102	60-61	0.34553527179198795
1102	62-63	0.3519094740588855
1102	64-65	0.29319709014918516
1102	66-67	0.2999055820310943
1102	68-69	0.3917488272617149
1102	70-71	0.3415686961385873
1102	72-73	0.364713061999133
1102	74-75	0.452658561082373
1102	76-77	0.2796340137185709
1102	78-79	0.31197117395954166
1102	80-81	0.4388808495377603
1102	82-83	0.34400052308598106
1102	84-85	0.3590916929951291
1102	86-87	0.3891370037769448
1102	88-89	0.4469644907656942
1102	90-91	0.47229921068425895
1102	92-93	0.4963775937567938
1102	94-95	0.4801010224677462
1102	96-97	0.48599771834390637
1102	98-99	0.56604579124701
1102	100	0.5856136673362826
1103	1	0.07654842559337638
1103	2	0.14328125536541592
1103	3	0.2319166658459615
1103	4	0.169949075641334
1103	5	0.12118324838517225
1103	6	0.2321143248552957
1103	7	0.2559137632681612
1103	8	0.24079742143856464
1103	9	0.3059569554565513
1103	10-11	0.28106571716286766
1103	12-13	0.2952635181598069
1103	14-15	0.42574532750809624
1103	16-17	0.385499150545094
1103	18-19	0.43206389349956
1103	20-21	0.41147991685161855
1103	22-23	0.45827863361559906
1103	24-25	0.4225167606332576
1103	26-27	0.394472169809049
1103	28-29	0.453824929275811
1103	30-31	0.451353837054306
1103	32-33	0.40702070755576614
1103	34-35	0.39448571768920715
1103	36-37	0.43439454386103193
1103	38-39	0.4338235120293703
1103	40-41	0.4746713861324281
1103	42-43	0.35757264602604977
1103	44-45	0.34687146179133777
1103	46-47	0.38895783433832065
1103	48-49	0.3232716788806371
1103	50-51	0.23076448224708201
1103	52-53	0.34215544061082426
1103	54-55	0.32351785363325547
1103	56-57	0.4195443097554943
1103	58-59	0.3426738505888096
1103	60-61	0.370464370475446
1103	62-63	0.28212419480037454
1103	64-65	0.3182692585822835
1103	66-67	0.2781958493638683
1103	68-69	0.33206666247401273
1103	70-71	0.3473177607819622
1103	72-73	0.4100183273303699
1103	74-75	0.3428623176634318
1103	76-77	0.40735287928733754
1103	78-79	0.3686021729194877
1103	80-81	0.379927170827159
1103	82-83	0.3533136179262186
1103	84-85	0.3697865805654459
1103	86-87	0.343140375917379
1103	88-89	0.3370132268656576
1103	90-91	0.3842500353737215
1103	92-93	0.37067208741065727
1103	94-95	0.2633675150492678
1103	96-97	0.24769699708759774
1103	98-99	0.33045258872135364
1103	100	0.2823479223111569
1104	1	0.07791189450042779
1104	2	0.11357378730783196
1104	3	0.13195797376607388
1104	4	0.15158582751799088
1104	5	0.12183451177301663
1104	6	0.17853526551044752
1104	7	0.23433814452343427
1104	8	0.2390949265178861
1104	9	0.26941328560395306
1104	10-11	0.26216681764989147
1104	12-13	0.35799571334555225
1104	14-15	0.3180615121195345
1104	16-17	0.3678844061427071
1104	18-19	0.4011909577625161
1104	20-21	0.35918772385428355
1104	22-23	0.31661035369552337
1104	24-25	0.21196667413266823
1104	26-27	0.31697575187907745
1104	28-29	0.3704287384099132
1104	30-31	0.3267726624590708
1104	32-33	0.2752852261889984
1104	34-35	0.40694506532190644
1104	36-37	0.41709936121916513
1104	38-39	0.3264276947476361
1104	40-41	0.47990188821142254
1104	42-43	0.4152515002557138
1104	44-45	0.4464786237947891
1104	46-47	0.45170265498555295
1104	48-49	0.41266213131242324
1104	50-51	0.4035599471813214
1104	52-53	0.47779481987839745
1104	54-55	0.4335496001942687
1104	56-57	0.5048161100427038
1104	58-59	0.6230814247650045
1104	60-61	0.5688073353093301
1104	62-63	0.585204467484445
1104	64-65	0.5454558185519289
1104	66-67	0.5162677423025457
1104	68-69	0.5574885714049458
1104	70-71	0.45913831671663985
1104	72-73	0.4690088537020429
1104	74-75	0.42909280323220855
1104	76-77	0.45232109064144055
1104	78-79	0.4845154016837867
1104	80-81	0.371822919478177
1104	82-83	0.4755090254307959
1104	84-85	0.4977140689791568
1104	86-87	0.46245940384749673
1104	88-89	0.4500471624874649
1104	90-91	0.4279456244907074
1104	92-93	0.4616481278458018
1104	94-95	0.5300902625050732
1104	96-97	0.5324027720384201
1104	98-99	0.4987720038322827
1104	100	0.5941684134998795
1105	1	0.0594558788530648
1105	2	0.11804299070247382
1105	3	0.2140037783574371
1105	4	0.18081992706981254
1105	5	0.2511152169947124
1105	6	0.2542763715563865
1105	7	0.2395631218169143
1105	8	0.25191435115392835
1105	9	0.2768789439318837
1105	10-11	0.3264932131559206
1105	12-13	0.3503859321335412
1105	14-15	0.35857030384779165
1105	16-17	0.3109270965645834
1105	18-19	0.45029275699848625
1105	20-21	0.40604365332219317
1105	22-23	0.4128196198134333
1105	24-25	0.34563584760302035
1105	26-27	0.30754700976441285
1105	28-29	0.35288942484683616
1105	30-31	0.33336707820456724
1105	32-33	0.30122451862540345
1105	34-35	0.2932277342082159
1105	36-37	0.31350180311464726
1105	38-39	0.3178736414638408
1105	40-41	0.368868255304875
1105	42-43	0.30906282798913054
1105	44-45	0.29820146147137194
1105	46-47	0.31765102538244605
1105	48-49	0.32542023595942027
1105	50-51	0.1701343111819753
1105	52-53	0.3291406999309814
1105	54-55	0.20527564094581408
1105	56-57	0.20717695285412674
1105	58-59	0.25028210608445534
1105	60-61	0.23510543409538798
1105	62-63	0.2537801492685503
1105	64-65	0.21330565846013627
1105	66-67	0.22773523852638533
1105	68-69	0.23425652783073048
1105	70-71	0.1399577060383237
1105	72-73	0.17760105579439056
1105	74-75	0.12471886343762151
1105	76-77	0.1821503534261737
1105	78-79	0.15619603714412023
1105	80-81	0.20730939655419434
1105	82-83	0.0691281454339645
1105	84-85	0.11533708378516394
1105	86-87	0.04879683323424899
1105	88-89	0.1449109435160878
1105	90-91	0.21491837171294748
1105	92-93	0.23330446919074532
1105	94-95	0.245613373589201
1105	96-97	0.23538634032587424
1105	98-99	0.24144860973758853
1105	100	0.2200017860713963
1106	1	-0.1351134463286705
1106	2	-0.11661418934747303
1106	3	-0.01474773063029744
1106	4	-0.08256869466360683
1106	5	0.0033717380806166375
1106	6	0.01888846846360792
1106	7	0.05228474207837763
1106	8	0.10630189387575228
1106	9	0.14032251435709497
1106	10-11	0.003728222301781159
1106	12-13	0.034042741306087976
1106	14-15	0.09542595689146083
1106	16-17	0.014210202100741753
1106	18-19	0.12651730908259395
1106	20-21	0.10938296832319594
1106	22-23	0.09676325485325776
1106	24-25	0.04207742075359988
1106	26-27	-0.031814219573362834
1106	28-29	0.03831157894062187
1106	30-31	0.05385316925129047
1106	32-33	-0.041267105113689695
1106	34-35	-0.007409901758279602
1106	36-37	0.006302106120429585
1106	38-39	0.1294140749806445
1106	40-41	0.1637523009574693
1106	42-43	0.09226082083845455
1106	44-45	0.12069065822235814
1106	46-47	0.08395098220096742
1106	48-49	0.12331043733640001
1106	50-51	0.2132196539599036
1106	52-53	0.18888994267917525
1106	54-55	0.13368553297110708
1106	56-57	0.09067206788390081
1106	58-59	0.18138401121758818
1106	60-61	0.1824547638369154
1106	62-63	0.25367692347565196
1106	64-65	0.23239139838157286
1106	66-67	0.21492788569799615
1106	68-69	0.3012976090696924
1106	70-71	0.34211662227781403
1106	72-73	0.327989216295002
1106	74-75	0.3386909604288917
1106	76-77	0.26950931757504293
1106	78-79	0.23363455607199768
1106	80-81	0.2925145400879501
1106	82-83	0.23631978398714182
1106	84-85	0.2525987582808824
1106	86-87	0.22972617005711982
1106	88-89	0.33814437459435
1106	90-91	0.2698644368928136
1106	92-93	0.3399627166952044
1106	94-95	0.32063199625768846
1106	96-97	0.24713910903600933
1106	98-99	0.18727844530126347
1106	100	0.20687661337175456
1107	1	0.06947271482101769
1107	2	0.14361794568500486
1107	3	0.18817253453995875
1107	4	0.15094044959086972
1107	5	0.05582928802216003
1107	6	0.17002359988765647
1107	7	0.18619854761076482
1107	8	0.14428331257295923
1107	9	0.1428193390583914
1107	10-11	0.24099623366621614
1107	12-13	0.22448494077340087
1107	14-15	0.21971542701538027
1107	16-17	0.19793530705218387
1107	18-19	0.1765285160191823
1107	20-21	0.1974109311444323
1107	22-23	0.18467864349164387
1107	24-25	0.10582217318126652
1107	26-27	0.05682154287189434
1107	28-29	0.09687690932313586
1107	30-31	0.2243485360411981
1107	32-33	0.12974468942984174
1107	34-35	0.18598446731138552
1107	36-37	0.2630711967835424
1107	38-39	0.2573003659692574
1107	40-41	0.2782629616821026
1107	42-43	0.22237890762254864
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2216	76-77	-0.8776319753590229
2216	78-79	-0.9888875755023818
2216	80-81	-0.9058547227473035
2216	82-83	-1.21922685418496
2216	84-85	-1.0121556246387513
2216	86-87	-0.9608747831202358
2216	88-89	-1.0725674856063065
2216	90-91	-0.942903532863177
2216	92-93	-1.1178268120819475
2216	94-95	-1.0928814476878657
2216	96-97	-1.1084765985242662
2216	98-99	-1.0446396640785096
2216	100	-1.0412573911413503
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34783.0
3	6486.0
4	3705.0
5	3107.0
6	2618.0
7	2910.0
8	3199.0
9	3632.0
10	4086.0
11	4555.0
12	5047.0
13	5565.0
14	5943.0
15	6005.0
16	6117.0
17	6069.0
18	5955.0
19	6067.0
20	6027.0
21	6397.0
22	7278.0
23	10055.0
24	14998.0
25	19252.0
26	19252.0
27	17716.0
28	19905.0
29	24375.0
30	29853.0
31	36629.0
32	47381.0
33	62662.0
34	90001.0
35	136197.0
36	245377.0
37	489519.0
38	744964.0
39	293233.0
40	451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65206561932041	19.988077873164826	13.53140868714147	23.828447820373288
2	15.27886151063855	20.22677187073248	40.73807578025688	23.75629083837209
3	17.824652612426803	25.562996121345083	30.155228302029315	26.4571229641988
4	12.091755934502748	16.990130410628623	39.60416528944623	31.3139483654224
5	12.712620303462868	38.0618062890437	36.07629609066105	13.149277316832384
6	30.355891103441053	38.803115202112394	18.04447766158622	12.79651603286033
7	27.10321094324281	32.62912147705853	22.934692888616944	17.332974691081716
8	24.73495911184966	36.86118810766987	20.636081703194456	17.767771077286017
9	24.79080447307779	17.24719837136534	21.308682040458773	36.653315115098096
10-11	23.495449399403686	27.209652912357683	29.33004242462949	19.964855263609138
12-13	24.28243721415472	24.549455469232935	29.105071950350457	22.06303536626189
14-15	21.602445136701057	26.56293222541243	26.023340351963814	25.811282285922697
16-17	21.320226408757275	29.893863966283813	26.624646659739977	22.16126296521894
18-19	21.353757038839237	28.899725366680862	28.355079073614625	21.391438520865275
20-21	22.545563933241343	28.214464649451344	27.82498439450687	21.414987022800446
22-23	22.213925037973645	27.909540621536188	27.913912722197136	21.96262161829303
24-25	22.05689328185379	28.027817338582512	27.870378900746072	22.044910478817627
26-27	21.83690217258386	27.93676774124732	27.94583122074793	22.280498865420896
28-29	21.82878768781554	28.002759780449242	28.052375886859675	22.11607664487554
30-31	22.100082017612742	28.448142593624798	27.63350958394795	21.818265804814505
32-33	21.814591085221586	28.099196797454383	28.12877928609921	21.957432831224818
34-35	21.910080544057113	27.8465062625087	28.155845999861295	22.08756719357289
36-37	22.19899880931831	28.221413838549637	27.62525197562643	21.954335376505618
38-39	21.970569202367734	28.338988785451964	27.850446413906827	21.83999559827347
40-41	21.754886930605778	27.997658601324314	28.137805807218957	22.109648660850947
42-43	21.789282298929674	27.932122020391482	28.049822023142536	22.228773657536312
44-45	21.921474335286128	28.039190567460103	28.087476657617135	21.95185843963664
46-47	21.89321109928519	28.293888954588457	27.887639775833073	21.925260170293285
48-49	21.81406378924621	28.09279996123857	27.917429115132748	22.17570713438248
50-51	21.854294710901453	27.949627984836084	27.92312439360974	22.27295291065272
52-53	21.864585392773314	27.964633782555577	27.98588258384602	22.184898240825085
54-55	21.976427521835152	28.34194914923693	27.798662115906435	21.88296121302148
56-57	21.80234724425953	28.02238645874627	27.848957506827432	22.326308790166777
58-59	21.742568485101046	27.713522939904315	28.18497674652532	22.358931828469313
60-61	22.01674086110989	28.14214459233154	27.723171452184303	22.117943094374265
62-63	21.82163631940531	28.088025020965425	27.855532049643667	22.2348066099856
64-65	21.813826823727645	27.880177709751415	27.988895809221088	22.317099657299856
66-67	21.69600689105571	27.943633266423635	27.864823796477367	22.495536046043288
68-69	22.052822538097992	28.122101619008166	27.795963831900366	22.029112010993472
70-71	21.990722161815533	27.841786974283032	27.904066192504366	22.26342467139707
72-73	21.85021465790724	27.96653327736388	27.987429452317002	22.195822612411874
74-75	21.88583573568667	28.016947983542202	27.857452522902072	22.239763757869056
76-77	21.841693174431114	28.00560280780805	27.797036973482108	22.355667044278725
78-79	21.913561431974244	28.05611230671498	27.880312355360154	22.15001390595062
80-81	21.909029187108807	27.842155471926127	27.99848859509126	22.250326745873807
82-83	21.722080003415442	27.882455472012268	28.15053883503914	22.24492568953315
84-85	21.712679254530194	27.907256913381325	27.928950218482573	22.451113613605912
86-87	21.8510240939129	27.988938143906744	27.889709805853137	22.27032795632722
88-89	21.67133813074257	27.861662545913372	27.99249911390293	22.474500209441125
90-91	21.638855083557885	27.754147110311184	28.05983167339467	22.547166132736262
92-93	21.805631719282577	27.90568951250107	27.89544845567326	22.3932303125431
94-95	21.64334587962944	27.68791738325583	28.064880905960244	22.603855831154494
96-97	21.65641619560062	27.673542250432483	28.10771727376259	22.562324280204308
98-99	21.604047770190157	27.752359150326196	28.01374925062767	22.629843828855982
100	21.750017754362545	27.610024708325586	27.967739194366555	22.672218342945317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	297.0
1	262.0
2	218.0
3	219.0
4	253.5
5	273.5
6	246.0
7	236.0
8	240.5
9	200.5
10	170.0
11	172.0
12	172.0
13	194.5
14	226.0
15	269.0
16	341.5
17	447.0
18	613.0
19	869.0
20	1283.0
21	1853.5
22	2850.5
23	4631.0
24	6746.5
25	9175.0
26	12673.0
27	17660.0
28	24273.0
29	31399.0
30	38273.0
31	45843.0
32	53308.5
33	60896.0
34	68955.0
35	75349.0
36	80295.0
37	85079.5
38	88380.5
39	89341.0
40	90332.5
41	91098.0
42	89546.5
43	87540.5
44	85940.5
45	84646.0
46	83221.0
47	80740.0
48	78501.0
49	76160.5
50	73806.5
51	71815.0
52	67753.5
53	64542.0
54	62845.5
55	59684.5
56	55271.5
57	50175.0
58	45661.0
59	41754.5
60	37152.5
61	31697.5
62	26828.5
63	22450.5
64	18261.0
65	14632.5
66	11315.0
67	8223.5
68	6063.5
69	4605.5
70	3213.5
71	2212.0
72	1675.5
73	1219.0
74	901.0
75	632.5
76	393.0
77	256.0
78	166.5
79	109.5
80	75.5
81	45.0
82	34.0
83	26.5
84	19.0
85	17.0
86	12.5
87	9.0
88	8.0
89	8.0
90	6.5
91	3.0
92	1.5
93	1.5
94	1.0
95	2.5
96	3.0
97	2.0
98	2.0
99	1.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06416749850556194
2	0.04472031545464355
3	0.06051602320697177
4	0.07836312157648548
5	0.09350238433131436
6	0.09091763215366064
7	0.09276388370912758
8	0.10494914397520935
9	0.10043608461740129
10-11	0.10595432537763025
12-13	0.11102126020207839
14-15	0.10915449474043959
16-17	0.07649635611484669
18-19	0.10207719711114967
20-21	0.09563583057318725
22-23	0.06035191195759693
24-25	0.022380671633493627
26-27	0.1858765038231767
28-29	0.06851644661399517
30-31	0.07871185798140702
32-33	0.07419879862359895
34-35	0.0233037974112271
36-37	0.056269644629397826
38-39	0.08053759563070209
40-41	0.08511219670702573
42-43	0.0796760115714842
44-45	0.07741948189258016
46-47	0.08381982061819887
48-49	0.08043502609984282
50-51	0.07598350846055033
52-53	0.07957344204062491
54-55	0.07114222660399258
56-57	0.07350132581375589
58-59	0.07030115645094653
60-61	0.0678600016164958
62-63	0.0744039376853175
64-65	0.07032167035711838
66-67	0.07179867160149193
68-69	0.07155250472742967
70-71	0.06427006803642121
72-73	0.06230073304392315
74-75	0.06420852631790565
76-77	0.05946981399220718
78-79	0.05594142213064814
80-81	0.050341125745731775
82-83	0.0564132419726008
84-85	0.04658708091628234
86-87	0.043940787020113066
88-89	0.04617680279284524
90-91	0.04250481358808323
92-93	0.04508956576573694
94-95	0.043530508896675965
96-97	0.0468332477903446
98-99	0.047817915286593626
100	0.05538754666400807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2437371.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.2949659146114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59698176093526	55.84965103944566
2	12.193364898241613	16.898776100161434
3	3.4798926398566903	7.234171255961293
4	1.394473979419672	3.8652010749079473
5	0.7034247644984121	2.4371897539705505
6	0.40508934335899754	1.684239134426043
7	0.2490667563823102	1.2081350675782505
8	0.19017453379473487	1.0542510269706613
9	0.15222620528638606	0.9493658735967683
>10	0.6065144002739575	7.126542009600772
>50	0.022914099489035403	1.0554722999926085
>100	0.005876618462928056	0.6370053633880542
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.051390617185484E-5	0.0
14-15	0.0	0.0	0.0	4.102781234370968E-5	0.0
16-17	0.0	0.0	0.0	4.102781234370968E-5	0.0
18-19	0.0	0.0	0.0	4.102781234370968E-5	0.0
20-21	0.0	0.0	0.0	4.102781234370968E-5	0.0
22-23	4.102781234370968E-5	0.0	0.0	8.205562468741935E-5	0.0
24-25	8.205562468741936E-5	0.0	0.0	1.2308343703112902E-4	0.0
26-27	1.0256953085927418E-4	0.0	0.0	1.6411124937483872E-4	0.0
28-29	1.2308343703112902E-4	0.0	0.0	6.359310913275E-4	0.0
30-31	1.4359734320298386E-4	0.0	0.0	0.0032001693628093547	0.0
32-33	1.6411124937483872E-4	0.0	0.0	0.00957999418225621	0.0
34-35	1.6411124937483872E-4	0.0	0.0	0.01780607055717	0.0
36-37	2.051390617185484E-4	0.0	0.0	0.033088930655201854	0.0
38-39	2.051390617185484E-4	0.0	0.0	0.06121349601681483	0.0
40-41	2.051390617185484E-4	0.0	0.0	0.09680512322498298	0.0
42-43	2.051390617185484E-4	0.0	0.0	0.13295062589979123	0.0
44-45	2.051390617185484E-4	0.0	0.0	0.17453231371014097	0.0
46-47	2.051390617185484E-4	0.0	0.0	0.22401185539665483	0.0
48-49	2.256529678904032E-4	0.0	0.0	0.282066209863004	0.0
50-51	2.8719468640596777E-4	0.0	0.0	0.347361973208018	0.0
52-53	2.8719468640596777E-4	4.102781234370968E-5	0.0	0.4139295987356869	0.0
54-55	2.8719468640596777E-4	4.102781234370968E-5	0.0	0.48468206112241424	0.0
56-57	2.8719468640596777E-4	4.102781234370968E-5	0.0	0.566553060654287	0.0
58-59	3.077085925778226E-4	4.102781234370968E-5	0.0	0.6641582262199723	0.0
60-61	3.2822249874967744E-4	4.102781234370968E-5	0.0	0.7713638998740857	0.0
62-63	3.2822249874967744E-4	4.102781234370968E-5	0.0	0.8852365930340518	0.0
64-65	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.0003196066581577	0.0
66-67	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.1300085214766238	0.0
68-69	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.2836166508914728	0.0
70-71	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.452323015248807	0.0
72-73	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.633645431901832	0.0
74-75	3.2822249874967744E-4	4.102781234370968E-5	0.0	1.8144344869943887	0.0
76-77	3.2822249874967744E-4	4.102781234370968E-5	0.0	2.0096448181257593	0.0
78-79	3.2822249874967744E-4	4.102781234370968E-5	0.0	2.2351131608606156	0.0
80-81	3.2822249874967744E-4	4.102781234370968E-5	0.0	2.4878034570855236	0.0
82-83	3.2822249874967744E-4	4.102781234370968E-5	0.0	2.7515097209247177	0.0
84-85	3.2822249874967744E-4	4.102781234370968E-5	0.0	3.00922592416173	0.0
86-87	3.2822249874967744E-4	4.102781234370968E-5	0.0	3.288276589817471	0.0
88	3.2822249874967744E-4	4.102781234370968E-5	0.0	3.5172322966015432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1680	0.0	34.975525	1
GTATCAA	2980	0.0	33.283554	1
ATCAACG	2920	0.0	33.001564	3
TCAACGC	2995	0.0	32.3321	4
CAACGCA	3040	0.0	31.699524	5
AACGCAG	3430	0.0	28.63811	6
GTACATG	6570	0.0	27.903763	1
TATCAAC	3705	0.0	26.51683	2
TACATGG	6700	0.0	26.169628	2
ACATGGG	7070	0.0	24.068703	3
CGCAGAG	4065	0.0	23.61211	8
ACGCAGA	4065	0.0	23.588345	7
CATGGGG	5015	0.0	21.933527	4
GCAGAGT	4985	0.0	18.503141	9
GTCGTAC	135	0.009985247	17.41004	1
GAGTACA	4980	0.0	17.179306	1
AGAGTAC	4190	0.0	16.405516	10-11
TCTATAC	875	0.0	16.116722	3
TACGCCC	235	5.6177276E-5	16.00242	4
GTATTAG	1155	0.0	15.872529	1
>>END_MODULE