FastQCFastQC Report
Wed 25 May 2016
SRR1033050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437371
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.14852068068422902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.1237809098409721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.056.314311
GTATCAA61300.042.8429341
ATCAACG72650.035.5796433
TCAACGC73250.035.2874834
CAACGCA74500.034.6323285
AACGCAG77700.033.2665146
TATCAAC78450.032.9860152
ACGCAGA87050.029.6393877
CGCAGAG87800.029.1732928
GCAGAGT99750.025.3956499
GTACATG65950.024.9514121
TGTCGCG1000.001759582523.499229
TACATGG66900.023.3212762
ACATGGG68850.021.9116763
AGAGTAC91350.021.3255110-11
CAGAGTA99100.021.27023310-11
GAGTACT68950.021.13055212-13
GTGGTAT17000.020.524251
TGGTATC16100.019.6155012
ATACCGC1456.606102E-419.4468336
Produced by FastQC (version 0.11.5)