##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033050_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2437371 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69868928447906 34.0 31.0 34.0 31.0 34.0 2 32.69054321233821 34.0 31.0 34.0 31.0 34.0 3 32.798854175256864 34.0 31.0 34.0 31.0 34.0 4 36.297321171048644 37.0 37.0 37.0 35.0 37.0 5 36.27918728827085 37.0 37.0 37.0 35.0 37.0 6 36.34499015537643 37.0 37.0 37.0 35.0 37.0 7 36.2931716181082 37.0 37.0 37.0 35.0 37.0 8 36.29225177455545 37.0 37.0 37.0 35.0 37.0 9 38.092567770766124 39.0 39.0 39.0 35.0 39.0 10-11 38.05457396514523 39.0 39.0 39.0 35.0 39.0 12-13 38.04146373284986 39.0 39.0 39.0 35.0 39.0 14-15 39.532167240850896 41.0 39.0 41.0 37.0 41.0 16-17 39.52728287979138 41.0 39.0 41.0 37.0 41.0 18-19 39.53905129748405 41.0 39.5 41.0 37.0 41.0 20-21 39.531845993080246 41.0 39.5 41.0 37.0 41.0 22-23 39.46765346760916 41.0 39.0 41.0 36.0 41.0 24-25 39.421280962151435 41.0 39.0 41.0 36.0 41.0 26-27 39.31322478194743 41.0 39.0 41.0 36.0 41.0 28-29 39.25836403239392 41.0 39.0 41.0 36.0 41.0 30-31 39.254433773110456 41.0 39.0 41.0 36.0 41.0 32-33 39.21882470908204 41.0 39.0 41.0 35.5 41.0 34-35 39.14583253841947 41.0 39.0 41.0 35.0 41.0 36-37 39.06114969776862 41.0 39.0 41.0 35.0 41.0 38-39 38.96851771847618 41.0 39.0 41.0 35.0 41.0 40-41 38.868185434224 41.0 39.0 41.0 35.0 41.0 42-43 38.75642977618098 41.0 39.0 41.0 35.0 41.0 44-45 38.60343049950131 41.0 38.5 41.0 35.0 41.0 46-47 38.41339972453927 41.0 38.0 41.0 34.5 41.0 48-49 38.222098113089885 40.0 38.0 41.0 34.0 41.0 50-51 38.087781671317174 40.0 38.0 41.0 34.0 41.0 52-53 37.95283545262498 40.0 38.0 41.0 34.0 41.0 54-55 37.807958451954995 40.0 37.5 41.0 33.0 41.0 56-57 37.662749331144084 40.0 37.0 41.0 33.0 41.0 58-59 37.4882966113899 40.0 37.0 41.0 33.0 41.0 60-61 37.2941817228481 40.0 36.0 41.0 33.0 41.0 62-63 37.06270834435956 39.0 36.0 41.0 32.5 41.0 64-65 36.796157006873386 39.0 35.0 41.0 32.0 41.0 66-67 36.518926334973216 39.0 35.0 41.0 32.0 41.0 68-69 36.21086798029516 38.0 35.0 40.5 31.5 41.0 70-71 35.88501524798646 37.0 35.0 40.0 31.0 41.0 72-73 35.54494125022411 37.0 35.0 39.5 31.0 41.0 74-75 35.196619226207254 36.0 35.0 39.0 31.0 41.0 76-77 34.837436935123954 36.0 35.0 39.0 31.0 40.5 78-79 34.48016592467869 35.0 34.5 37.5 30.0 39.5 80-81 34.1450489072037 35.0 34.0 37.0 30.0 39.0 82-83 33.84145458364771 35.0 34.0 37.0 30.0 39.0 84-85 33.616354670667704 35.0 34.0 36.0 30.0 37.5 86-87 33.40301496981789 35.0 34.0 36.0 30.0 37.0 88-89 33.18923565595882 35.0 34.0 36.0 30.0 37.0 90-91 33.00506508857289 35.0 34.0 35.0 30.0 36.0 92-93 32.82871852500091 35.0 34.0 35.0 29.0 36.0 94-95 32.67098648502834 35.0 34.0 35.0 29.0 36.0 96-97 32.52519784636807 35.0 34.0 35.0 29.0 36.0 98-99 32.400868804954186 35.0 34.0 35.0 29.0 35.5 100 32.300497954558416 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1606046988552876 1101 2 0.11794596479170139 1101 3 0.17416721630436882 1101 4 0.13938291940407055 1101 5 0.10353375906132811 1101 6 0.1372021447735321 1101 7 0.1785244724330184 1101 8 0.19653636421527665 1101 9 0.2516350229790092 1101 10-11 0.26259640548438057 1101 12-13 0.2522756262301087 1101 14-15 0.30853937851305346 1101 16-17 0.2788041401483454 1101 18-19 0.2727913344169224 1101 20-21 0.308549351098371 1101 22-23 0.33580340474551207 1101 24-25 0.3710068052485056 1101 26-27 0.3576742122121246 1101 28-29 0.36022874714534936 1101 30-31 0.3412861827230387 1101 32-33 0.30192131498893815 1101 34-35 0.27688815018247936 1101 36-37 0.3304007793598416 1101 38-39 0.3164538338625249 1101 40-41 0.35951018181250305 1101 42-43 0.34982597503481827 1101 44-45 0.3614648609054356 1101 46-47 0.3541803736019915 1101 48-49 0.352546677179987 1101 50-51 0.3425912389414876 1101 52-53 0.36379135279633346 1101 54-55 0.33098970013114126 1101 56-57 0.30676085421470844 1101 58-59 0.25512030544324915 1101 60-61 0.3183437498510173 1101 62-63 0.3021199477467462 1101 64-65 0.3466971182042755 1101 66-67 0.30804729439192613 1101 68-69 0.3102831640894692 1101 70-71 0.29943278050826194 1101 72-73 0.404721277218961 1101 74-75 0.3570548999623071 1101 76-77 0.40662056157847815 1101 78-79 0.35756671222711134 1101 80-81 0.30909990474817306 1101 82-83 0.3043514638792004 1101 84-85 0.25184331682098815 1101 86-87 0.2783199159819887 1101 88-89 0.3295044098296245 1101 90-91 0.3464100570931734 1101 92-93 0.2978674694686845 1101 94-95 0.26381213251077895 1101 96-97 0.3388805761800384 1101 98-99 0.36681259850310255 1101 100 0.31044090716876127 1102 1 0.1863031798885686 1102 2 0.1547574898955233 1102 3 0.1962355761264689 1102 4 0.11653987391512288 1102 5 0.1591348786251885 1102 6 0.12848223515879198 1102 7 0.17057406987032664 1102 8 0.1677248334939776 1102 9 0.19017320358943124 1102 10-11 0.20202276242189754 1102 12-13 0.2149082338785817 1102 14-15 0.30054974136016455 1102 16-17 0.24705541320985702 1102 18-19 0.2572137099651499 1102 20-21 0.29488597089184765 1102 22-23 0.2588093835001999 1102 24-25 0.3330470759127522 1102 26-27 0.3053870989229992 1102 28-29 0.2990851492474107 1102 30-31 0.26274147354702393 1102 32-33 0.36543211038205214 1102 34-35 0.37747300011851337 1102 36-37 0.3468710565684603 1102 38-39 0.3760122051021497 1102 40-41 0.3723486262338511 1102 42-43 0.3944741487216916 1102 44-45 0.36952774473734706 1102 46-47 0.4312272342730239 1102 48-49 0.4911622649159 1102 50-51 0.5165820761286497 1102 52-53 0.48672969357517104 1102 54-55 0.4580381494958701 1102 56-57 0.4902698537695258 1102 58-59 0.5194914004165909 1102 60-61 0.4904205183391994 1102 62-63 0.4716038101297144 1102 64-65 0.5451275941593536 1102 66-67 0.5018170170147869 1102 68-69 0.5717129813949597 1102 70-71 0.5393658605166323 1102 72-73 0.5415027136929851 1102 74-75 0.5718003824547253 1102 76-77 0.4955982924815032 1102 78-79 0.5241702992692652 1102 80-81 0.5630212572127746 1102 82-83 0.5426776372655411 1102 84-85 0.5282104974637107 1102 86-87 0.5568112942027312 1102 88-89 0.6293623577921039 1102 90-91 0.5907393860991732 1102 92-93 0.584439988332889 1102 94-95 0.6192748426221044 1102 96-97 0.6450504217554922 1102 98-99 0.6317577736491131 1102 100 0.6936869296414869 1103 1 0.11534626278401561 1103 2 0.08605604027179226 1103 3 0.15142448625450555 1103 4 0.09215199080412617 1103 5 0.10226194092096108 1103 6 0.11409923320115922 1103 7 0.12919411128088143 1103 8 0.12669534493696943 1103 9 0.17155650668854605 1103 10-11 0.21699160449179544 1103 12-13 0.1891870390190249 1103 14-15 0.28560641113542573 1103 16-17 0.2777660384526328 1103 18-19 0.2559388805194658 1103 20-21 0.34981513483816684 1103 22-23 0.283715724939718 1103 24-25 0.23703210240905292 1103 26-27 0.2463732427703036 1103 28-29 0.29118533434814253 1103 30-31 0.24733325139293072 1103 32-33 0.19668121354745693 1103 34-35 0.22663544454655238 1103 36-37 0.28509458966066603 1103 38-39 0.2930496340302611 1103 40-41 0.3067328808993679 1103 42-43 0.30352628535568016 1103 44-45 0.34235792628421535 1103 46-47 0.266250098734524 1103 48-49 0.2513812209627204 1103 50-51 0.3372931768579761 1103 52-53 0.2702137148962933 1103 54-55 0.28472342935614847 1103 56-57 0.2541461574269235 1103 58-59 0.2205199091831318 1103 60-61 0.26112022349515485 1103 62-63 0.1658166726595951 1103 64-65 0.18129444027062647 1103 66-67 0.21281671604281627 1103 68-69 0.12665155336391365 1103 70-71 0.1985788022942785 1103 72-73 0.1620686003215681 1103 74-75 0.14876868699597878 1103 76-77 0.10821202678758368 1103 78-79 0.11564458811810141 1103 80-81 0.17582980539299342 1103 82-83 0.1738484843291559 1103 84-85 0.18292140376056665 1103 86-87 0.1837468783193117 1103 88-89 0.2147026158821319 1103 90-91 0.13903720781986095 1103 92-93 0.29878169669927246 1103 94-95 0.3303101819521004 1103 96-97 0.2897597524204585 1103 98-99 0.29615644293093624 1103 100 0.3698699948447626 1104 1 0.12048733451087656 1104 2 0.12539924434198468 1104 3 0.09267060635307445 1104 4 0.060084208909941594 1104 5 0.09951209922482462 1104 6 0.10183594441735977 1104 7 0.10986525815190618 1104 8 0.08476667296717721 1104 9 0.12505264264986948 1104 10-11 0.16023651319351728 1104 12-13 0.18636747402120335 1104 14-15 0.18976564280811914 1104 16-17 0.212041770761779 1104 18-19 0.21823743156971176 1104 20-21 0.2644961509353081 1104 22-23 0.24450743525595442 1104 24-25 0.1979399912506139 1104 26-27 0.21552952364603328 1104 28-29 0.25943715468069684 1104 30-31 0.23813912326845355 1104 32-33 0.2534877374147513 1104 34-35 0.27356112849045644 1104 36-37 0.26130372848765404 1104 38-39 0.3054234360682031 1104 40-41 0.3007844270975468 1104 42-43 0.34665652087556964 1104 44-45 0.3362265651558687 1104 46-47 0.26995818058377097 1104 48-49 0.2082369436635645 1104 50-51 0.23776055363670423 1104 52-53 0.30363408028362215 1104 54-55 0.2756935536295373 1104 56-57 0.30040451289259096 1104 58-59 0.2798865690946357 1104 60-61 0.31197533952638423 1104 62-63 0.26433994688850504 1104 64-65 0.30006919750566396 1104 66-67 0.3073948977562253 1104 68-69 0.29670955926616216 1104 70-71 0.25043128931595504 1104 72-73 0.3030446245702194 1104 74-75 0.289164749341154 1104 76-77 0.25257248403647736 1104 78-79 0.19302664850278006 1104 80-81 0.1721891402707385 1104 82-83 0.2055184023219283 1104 84-85 0.2266083735721125 1104 86-87 0.22035853142602235 1104 88-89 0.24999248029114796 1104 90-91 0.2490869618499687 1104 92-93 0.21638024651934984 1104 94-95 0.23070819962515543 1104 96-97 0.24333564730668655 1104 98-99 0.2655228244348251 1104 100 0.33547872337662454 1105 1 0.056149593490331995 1105 2 0.07037357637384645 1105 3 0.05871568854880849 1105 4 0.04033240385020065 1105 5 0.05400713786617928 1105 6 0.08496504816353223 1105 7 0.0889051751569454 1105 8 0.09374008434732417 1105 9 0.16603690049678477 1105 10-11 0.1186012628890083 1105 12-13 0.14277223768136338 1105 14-15 0.17944912386288792 1105 16-17 0.1923066161158289 1105 18-19 0.18193678023649795 1105 20-21 0.17178850556092584 1105 22-23 0.21209728863318134 1105 24-25 0.19221313950774999 1105 26-27 0.22131012137575823 1105 28-29 0.254214345813196 1105 30-31 0.21965518913656013 1105 32-33 0.2071628399813079 1105 34-35 0.19195264895358832 1105 36-37 0.21711163289599966 1105 38-39 0.16715681229506174 1105 40-41 0.1904604115939179 1105 42-43 0.1323362279398097 1105 44-45 0.10206591019209554 1105 46-47 0.136971945132359 1105 48-49 0.16671050187711955 1105 50-51 0.20098571400729526 1105 52-53 0.26432953941374393 1105 54-55 0.21628805239477344 1105 56-57 0.23873219334590345 1105 58-59 0.2040248443533912 1105 60-61 0.221333816894834 1105 62-63 0.2647122966072928 1105 64-65 0.2804022708344007 1105 66-67 0.22263914151456277 1105 68-69 0.30768721580483316 1105 70-71 0.37553378616553346 1105 72-73 0.33306001574806743 1105 74-75 0.2913299614070226 1105 76-77 0.3085598876499418 1105 78-79 0.33305790636332233 1105 80-81 0.27765766277961745 1105 82-83 0.32583529919283194 1105 84-85 0.3516223521753332 1105 86-87 0.2623856472831605 1105 88-89 0.21435556091817887 1105 90-91 0.23048860452558273 1105 92-93 0.25994353729783626 1105 94-95 0.19041160386808542 1105 96-97 0.19968875596217828 1105 98-99 0.23007947580272514 1105 100 0.2337722650605869 1106 1 0.03826132529868431 1106 2 0.07130076988254785 1106 3 0.0849135017379723 1106 4 0.05293803299170463 1106 5 0.043836901986047394 1106 6 0.05117422932296023 1106 7 0.050762588029648725 1106 8 0.07617226263744215 1106 9 0.05604023103890654 1106 10-11 0.06949677947500987 1106 12-13 0.11237788888639244 1106 14-15 0.10337959433085331 1106 16-17 0.13113916129090342 1106 18-19 0.131461731460341 1106 20-21 0.10350083497571205 1106 22-23 0.08281289290594174 1106 24-25 0.09387940868069222 1106 26-27 0.15285513570128728 1106 28-29 0.11308525000810477 1106 30-31 0.13995962380067795 1106 32-33 0.1541620425423602 1106 34-35 0.07259999287251162 1106 36-37 0.09021088620317386 1106 38-39 0.053854553515094494 1106 40-41 0.09700324583273101 1106 42-43 0.06316680420764698 1106 44-45 0.08246036405148516 1106 46-47 0.01643495037402687 1106 48-49 0.04815292079628364 1106 50-51 0.0797765696204138 1106 52-53 0.04287843166703453 1106 54-55 0.0780697663526837 1106 56-57 -0.02471132454709135 1106 58-59 -0.008785765472019591 1106 60-61 0.04979041005493201 1106 62-63 0.031357652707384887 1106 64-65 -0.007150904895297572 1106 66-67 -0.057221217364478605 1106 68-69 -0.059272335444020996 1106 70-71 0.005668941035750663 1106 72-73 -0.03439543956435642 1106 74-75 0.006098709364827926 1106 76-77 0.007724730573386296 1106 78-79 -4.1236093107244187E-4 1106 80-81 -0.015636495533549066 1106 82-83 -0.006423312675039483 1106 84-85 -0.008834762104292793 1106 86-87 -0.07131234714212553 1106 88-89 -0.03893873397873193 1106 90-91 -0.0074980670020678986 1106 92-93 -0.04716081373500458 1106 94-95 -0.013503201494820871 1106 96-97 -0.016935429657230827 1106 98-99 0.020759106017997908 1106 100 -0.019473437541961403 1107 1 0.03861974534274992 1107 2 -0.004604518378030775 1107 3 0.016692537295256216 1107 4 0.048466364329328826 1107 5 0.054261372281608544 1107 6 0.08736152853201418 1107 7 0.08332126290531505 1107 8 0.07154208144854124 1107 9 0.04158530210703759 1107 10-11 0.06548398943241551 1107 12-13 0.07613354536047012 1107 14-15 0.12965127874351623 1107 16-17 0.12410605135590203 1107 18-19 0.10250290635114823 1107 20-21 0.11801381785837606 1107 22-23 0.16198434692014274 1107 24-25 0.15358209168189063 1107 26-27 0.10758567450252343 1107 28-29 0.13216076860657466 1107 30-31 0.12967700707184804 1107 32-33 0.1263925661990939 1107 34-35 0.15706192866382906 1107 36-37 0.07803924439705412 1107 38-39 0.1814732915130719 1107 40-41 0.16313123914492422 1107 42-43 0.17917879058162356 1107 44-45 0.17306844637299434 1107 46-47 0.09353766447195255 1107 48-49 0.0846426053664544 1107 50-51 0.17712799693989467 1107 52-53 0.16013580148987927 1107 54-55 0.13897896189656933 1107 56-57 0.14867260490776602 1107 58-59 0.21947176791152145 1107 60-61 0.24905024742309223 1107 62-63 0.23616896222278427 1107 64-65 0.19875989596651777 1107 66-67 0.22497398929748869 1107 68-69 0.2209005744623056 1107 70-71 0.1939621346895919 1107 72-73 0.16592451342709325 1107 74-75 0.16909148644371896 1107 76-77 0.1601951028657993 1107 78-79 0.16627062815074112 1107 80-81 0.1968147139069245 1107 82-83 0.17115009275961768 1107 84-85 0.17677532938873952 1107 86-87 0.188679495015748 1107 88-89 0.1759941556541449 1107 90-91 0.1063189752147693 1107 92-93 0.13007032579842814 1107 94-95 0.11020270021933243 1107 96-97 0.2144903349890157 1107 98-99 0.19130949785692763 1107 100 0.2233142425010044 1108 1 0.05181557476869614 1108 2 0.04831948585667334 1108 3 0.06693243065569732 1108 4 0.04648506360280891 1108 5 0.0032403712547477426 1108 6 0.025380604076254087 1108 7 0.004156661462509703 1108 8 0.009551335219235568 1108 9 -0.0028591915231217513 1108 10-11 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-0.20826089715381357 2214 64-65 -0.24064402617611336 2214 66-67 -0.19560490549423548 2214 68-69 -0.1478713774201026 2214 70-71 -0.19145190087878916 2214 72-73 -0.2534388999666888 2214 74-75 -0.25277660741198105 2214 76-77 -0.2660230213589472 2214 78-79 -0.23680127487845937 2214 80-81 -0.1591740567376192 2214 82-83 -0.1795922131740184 2214 84-85 -0.11401093191521028 2214 86-87 -0.16813274292998415 2214 88-89 -0.19769082689694528 2214 90-91 -0.2024206810113256 2214 92-93 -0.23621308406741548 2214 94-95 -0.30219878802055433 2214 96-97 -0.19151103990643747 2214 98-99 -0.2000950285391241 2214 100 -0.21883537339773795 2215 1 -0.2232086369317301 2215 2 -0.16889920757978416 2215 3 -0.2158508796347789 2215 4 -0.14263002379308176 2215 5 -0.17662818425850446 2215 6 -0.12972522610120762 2215 7 -0.162450561305171 2215 8 -0.15495731754156594 2215 9 -0.24262181667256755 2215 10-11 -0.23098106967204757 2215 12-13 -0.26892882721119094 2215 14-15 -0.3687732211679631 2215 16-17 -0.37348929010560994 2215 18-19 -0.3613394470822868 2215 20-21 -0.31933526428624504 2215 22-23 -0.42436022548390184 2215 24-25 -0.43860781258980097 2215 26-27 -0.44519890151624963 2215 28-29 -0.4600256694906477 2215 30-31 -0.47483223832959 2215 32-33 -0.43603261704394214 2215 34-35 -0.45167889680561757 2215 36-37 -0.44773380535368545 2215 38-39 -0.44906420073445474 2215 40-41 -0.42082581990507606 2215 42-43 -0.43487988927630994 2215 44-45 -0.424999223256016 2215 46-47 -0.4900634308391858 2215 48-49 -0.48035342710169715 2215 50-51 -0.43844329669831694 2215 52-53 -0.42666457285695714 2215 54-55 -0.47421824981366 2215 56-57 -0.4491256934481527 2215 58-59 -0.3753146105179823 2215 60-61 -0.37143051097587687 2215 62-63 -0.39972185080557665 2215 64-65 -0.3648106722508828 2215 66-67 -0.3446885662386734 2215 68-69 -0.308052009508863 2215 70-71 -0.32840199436598994 2215 72-73 -0.386126471523518 2215 74-75 -0.32009974363970883 2215 76-77 -0.29980196326509656 2215 78-79 -0.28767313564431873 2215 80-81 -0.3294352443132951 2215 82-83 -0.3452988857711432 2215 84-85 -0.3163699392212109 2215 86-87 -0.2447317034512082 2215 88-89 -0.23221807820864626 2215 90-91 -0.23865005823009966 2215 92-93 -0.25582686251512143 2215 94-95 -0.1894581717463666 2215 96-97 -0.1952847102878863 2215 98-99 -0.26390065753911074 2215 100 -0.2063039707603309 2216 1 -0.2528933709907051 2216 2 -0.2216115822848721 2216 3 -0.23542563456797438 2216 4 -0.20995860448739734 2216 5 -0.18240867351408951 2216 6 -0.21682370882545143 2216 7 -0.2076742723855034 2216 8 -0.1656385763394681 2216 9 -0.2771957077470688 2216 10-11 -0.3284030887222471 2216 12-13 -0.326418482149208 2216 14-15 -0.3917177331161241 2216 16-17 -0.39077467786487574 2216 18-19 -0.3722327053023946 2216 20-21 -0.3888948662881333 2216 22-23 -0.464478814873452 2216 24-25 -0.4153034539269882 2216 26-27 -0.3997005966619298 2216 28-29 -0.4362507202600483 2216 30-31 -0.46710551790542354 2216 32-33 -0.4046517459911385 2216 34-35 -0.42865493818020184 2216 36-37 -0.39457916331691223 2216 38-39 -0.48113049410086717 2216 40-41 -0.4936770067902856 2216 42-43 -0.5444875558098587 2216 44-45 -0.5250785283170956 2216 46-47 -0.4723815177745223 2216 48-49 -0.4273333204363894 2216 50-51 -0.46327923435320173 2216 52-53 -0.46684929700280264 2216 54-55 -0.40024914479959506 2216 56-57 -0.36678197398413914 2216 58-59 -0.45307194836036757 2216 60-61 -0.4205471092419302 2216 62-63 -0.40053699354051986 2216 64-65 -0.48592422860138385 2216 66-67 -0.5047100352516338 2216 68-69 -0.5614383356169412 2216 70-71 -0.5014042857157861 2216 72-73 -0.521429402695226 2216 74-75 -0.5670678828314166 2216 76-77 -0.49790628907295087 2216 78-79 -0.5976964456676299 2216 80-81 -0.5834097024189759 2216 82-83 -0.5553469786260479 2216 84-85 -0.6495415468764207 2216 86-87 -0.6180490908026144 2216 88-89 -0.7125505646772154 2216 90-91 -0.7210780393215046 2216 92-93 -0.6610214856319772 2216 94-95 -0.5602543662363644 2216 96-97 -0.6171917420977096 2216 98-99 -0.7695730936489653 2216 100 -0.8207187985408915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 6.0 4 5.0 5 17.0 6 50.0 7 117.0 8 308.0 9 501.0 10 983.0 11 1595.0 12 2617.0 13 3869.0 14 4664.0 15 6013.0 16 7104.0 17 10378.0 18 12045.0 19 6364.0 20 4562.0 21 4440.0 22 4587.0 23 4956.0 24 5718.0 25 7075.0 26 8977.0 27 11815.0 28 15167.0 29 19814.0 30 25689.0 31 32392.0 32 41670.0 33 54566.0 34 74972.0 35 113519.0 36 206501.0 37 461565.0 38 850477.0 39 430649.0 40 1609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4375811686979 20.028165846017952 13.802742209036383 23.73151077624776 2 15.504712237834859 20.395239848073256 40.270005161560974 23.830042752530915 3 17.823994485724015 25.711829845198192 30.52283049755673 25.941345171521064 4 12.17845725126697 17.176955596985533 39.33296188588084 31.31162526586666 5 12.406779605385971 37.813437358069294 36.11984890264871 13.659934133896018 6 30.22454530908315 38.770108764775145 17.770793929522394 13.234551996619306 7 26.737004748148724 32.789386597280426 22.97467230060586 17.49893635396499 8 24.96543399719205 36.23883323226795 20.518215155212634 18.277517615327366 9 25.069655025562398 16.356940619167634 21.495514220456567 37.0778901348134 10-11 23.692050667208782 26.697295490953206 29.288936308224223 20.321717533613786 12-13 24.757474344283246 24.021455904743267 29.385801340870966 21.835268410102522 14-15 21.873938402306756 25.8782233593341 26.861498733878076 25.38633950448107 16-17 21.003959597418365 29.427078952756027 27.29338479319299 22.275576656632623 18-19 20.897704525509354 27.965040878228542 29.908167070860657 21.22908752540145 20-21 22.010461684015624 27.309922722048206 29.57420712197838 21.105408471957794 22-23 21.689293186483702 27.08427141812984 29.69899709461249 21.527438300773973 24-25 21.523660688276617 27.210837743206305 29.609975489612832 21.655526078904245 26-27 21.314298597032007 27.210267959071434 29.698956066758065 21.7764773771385 28-29 21.349548672068952 27.202246854489875 29.741589578573237 21.706614894867936 30-31 21.6130839468878 27.675277661133308 29.276451221881306 21.435187170097585 32-33 21.32295411736662 27.351970627368587 29.735994233130697 21.589081022134092 34-35 21.429687971178783 27.128615216969433 29.67664750257552 21.765049309276264 36-37 21.765603184742904 27.528328678728027 29.154199340190722 21.55186879633835 38-39 21.60075343474588 27.68029569564912 29.219023283693783 21.499927585911212 40-41 21.46880949679952 27.353311573526938 29.403637570601244 21.774241359072295 42-43 21.494942706711452 27.304952754422697 29.257856108077107 21.942248430788748 44-45 21.63548347789483 27.501168266956487 29.116063988617242 21.747284266531437 46-47 21.72408303865107 27.778064972464183 28.759532299350404 21.73831968953434 48-49 21.738278661721992 27.64476561015947 28.58319476189714 22.03376096622139 50-51 21.764577489434313 27.632293155206984 28.451126233962743 22.152003121395964 52-53 21.80866187379763 27.656417508865083 28.43186367606737 22.103056941269916 54-55 21.919785703530568 28.036273509449323 28.212508477371728 21.831432309648388 56-57 21.66920833964136 27.725385261414857 28.327263268497084 22.278143130446697 58-59 21.597204528978146 27.383541528967072 28.737131113810744 22.282122828244038 60-61 21.86938303606632 27.78544997868605 28.242151071790055 22.103015913457575 62-63 21.694953291887035 27.671372146464368 28.426571088275033 22.207103473373564 64-65 21.744555917010583 27.471689783787532 28.524955782275246 22.25879851692664 66-67 21.569863916872713 27.60542560107296 28.34158778897176 22.483122693082567 68-69 21.93993433895443 27.71032335726354 28.275995480366923 22.073746823415114 70-71 21.951602772003113 27.472489826128232 28.32966339551919 22.246244006349464 72-73 21.79139020679718 27.627223508675762 28.338668331853594 22.242717952673463 74-75 21.860240226342516 27.692453499020253 28.185259673549503 22.262046601087732 76-77 21.853648870032508 27.67748529050358 28.06175178091476 22.407114058549148 78-79 21.938822608458047 27.76518223938826 28.166188077235677 22.129807074918016 80-81 21.859495333291484 27.59528606847296 28.256756972984416 22.28846162525114 82-83 21.698688565147208 27.660220438559048 28.358309381613818 22.28278161467993 84-85 21.660428387799804 27.644704068440955 28.165634201769034 22.529233341990203 86-87 21.783101754172908 27.682436669682907 28.112020405689027 22.42244117045516 88-89 21.53219961519202 27.592320169760264 28.199872852122997 22.675607362924723 90-91 21.49685915718615 27.532229513234498 28.223151337932777 22.747759991646575 92-93 21.612276091854472 27.641843793406416 28.0732129555988 22.672667159140307 94-95 21.4011266288115 27.413451439262154 28.266546591393965 22.91887534053238 96-97 21.4218633647402 27.383834591864577 28.258693301988007 22.935608741407215 98-99 21.381125616290035 27.455344230064654 28.172614136484164 22.99091601716114 100 21.448437681419858 27.325630771843922 28.10528228981144 23.12064925692478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 14.0 2 52.0 3 98.0 4 74.0 5 60.5 6 135.0 7 320.5 8 591.0 9 721.5 10 632.5 11 581.5 12 667.0 13 782.0 14 925.0 15 1090.0 16 1284.0 17 1501.0 18 1716.0 19 2036.5 20 2528.5 21 3160.0 22 4152.0 23 5703.0 24 7606.5 25 9946.0 26 13290.5 27 18075.5 28 24388.0 29 31403.5 30 38030.0 31 45352.5 32 53092.5 33 60771.5 34 68540.5 35 74886.5 36 79890.0 37 84499.0 38 87749.0 39 88783.5 40 89660.0 41 90390.5 42 88632.0 43 86961.0 44 85910.0 45 84492.0 46 83435.0 47 81200.0 48 78739.0 49 76101.5 50 73186.0 51 70713.0 52 66946.0 53 63855.0 54 62230.5 55 59543.0 56 55151.0 57 50067.5 58 45737.0 59 41983.0 60 37251.0 61 31324.5 62 26329.0 63 22199.0 64 18005.5 65 14207.0 66 10743.0 67 7813.0 68 5832.0 69 4310.0 70 2931.0 71 1988.0 72 1471.5 73 1021.0 74 716.0 75 481.5 76 289.5 77 186.0 78 108.0 79 45.5 80 21.5 81 12.5 82 7.0 83 3.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04459723201761242 2 0.24354109407226066 3 0.0025026965529662904 4 0.002420640928278871 5 8.205562468741935E-5 6 4.102781234370967E-5 7 0.0 8 2.051390617185484E-4 9 8.205562468741935E-5 10-11 6.359310913275E-4 12-13 0.0 14-15 2.8719468640596777E-4 16-17 8.205562468741935E-5 18-19 4.102781234370968E-4 20-21 2.0513906171854837E-5 22-23 1.025695308592742E-4 24-25 0.001518029056717258 26-27 1.025695308592742E-4 28-29 3.282224987496774E-4 30-31 1.025695308592742E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.025695308592742E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0513906171854837E-5 68-69 2.051390617185484E-4 70-71 0.0 72-73 2.8719468640596777E-4 74-75 1.2308343703112905E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.025695308592742E-4 84-85 0.0 86-87 4.7181984195266124E-4 88-89 0.002112932335701048 90-91 0.0019488210863262097 92-93 6.359310913275E-4 94-95 4.513059357808064E-4 96-97 2.461668740622581E-4 98-99 1.641112493748387E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2437371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.10267704855475 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18093747931316 53.132528760761566 2 13.09270221312161 17.571107365999953 3 3.716222439876451 7.48105422670865 4 1.4915580984176398 4.003501655091013 5 0.7478642067389227 2.5091845170487765 6 0.44161199961719777 1.778000843464759 7 0.283297802501382 1.3307028664870841 8 0.18800352109570564 1.009243164806103 9 0.14595733112977666 0.8814714888303334 >10 0.6760327662603287 7.76190447856224 >50 0.027481911328525937 1.242821352288407 >100 0.008085951373843547 0.9257709554943798 >500 6.106980634598829E-5 0.038684624330640856 >1k 1.832094190379649E-4 0.3340237001261161 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3620 0.14852068068422902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3017 0.1237809098409721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.102781234370968E-5 0.0 10-11 0.0 0.0 0.0 4.102781234370968E-5 0.0 12-13 0.0 0.0 0.0 4.102781234370968E-5 0.0 14-15 0.0 0.0 0.0 4.102781234370968E-5 0.0 16-17 0.0 0.0 0.0 4.102781234370968E-5 0.0 18-19 0.0 0.0 0.0 4.102781234370968E-5 0.0 20-21 0.0 0.0 0.0 4.102781234370968E-5 0.0 22-23 0.0 0.0 0.0 8.205562468741935E-5 0.0 24-25 0.0 0.0 0.0 1.4359734320298386E-4 0.0 26-27 0.0 0.0 0.0 2.051390617185484E-4 0.0 28-29 0.0 0.0 0.0 6.769589036712097E-4 0.0 30-31 0.0 0.0 0.0 0.0032001693628093547 0.0 32-33 0.0 0.0 0.0 0.00957999418225621 0.0 34-35 0.0 0.0 0.0 0.01784709836951371 0.0 36-37 0.0 2.051390617185484E-5 0.0 0.03333509752926411 0.0 38-39 0.0 4.102781234370968E-5 0.0 0.06133657945384597 0.0 40-41 0.0 4.102781234370968E-5 0.0 0.09688717884967041 0.0 42-43 0.0 4.102781234370968E-5 0.0 0.1335045013664313 0.0 44-45 4.102781234370968E-5 4.102781234370968E-5 0.0 0.1753323560508433 0.0 46-47 4.102781234370968E-5 4.102781234370968E-5 0.0 0.2250170367990757 0.0 48-49 4.102781234370968E-5 4.102781234370968E-5 0.0 0.28327653032714345 0.0 50-51 8.205562468741936E-5 4.102781234370968E-5 0.0 0.348921030077079 0.0 52-53 8.205562468741936E-5 4.102781234370968E-5 0.0 0.41596047544670056 0.0 54-55 8.205562468741936E-5 4.102781234370968E-5 0.0 0.48724629939389613 0.0 56-57 8.205562468741936E-5 4.102781234370968E-5 0.0 0.5695891187677214 0.0 58-59 1.2308343703112902E-4 4.102781234370968E-5 0.0 0.6675019929259847 0.0 60-61 1.2308343703112902E-4 4.102781234370968E-5 0.0 0.7753025698590817 0.0 62-63 1.2308343703112902E-4 4.102781234370968E-5 0.0 0.8902214722338125 0.0 64-65 1.2308343703112902E-4 4.102781234370968E-5 0.0 1.0063917228850265 0.0 66-67 1.2308343703112902E-4 4.102781234370968E-5 0.0 1.1370037634812262 0.0 68-69 1.2308343703112902E-4 4.102781234370968E-5 0.0 1.291350393518262 0.0 70-71 2.051390617185484E-4 4.102781234370968E-5 0.0 1.4614722174014543 0.0 72-73 2.051390617185484E-4 4.102781234370968E-5 0.0 1.645071677639555 0.0 74-75 2.051390617185484E-4 4.102781234370968E-5 0.0 1.8273992756950008 0.0 76-77 2.051390617185484E-4 4.102781234370968E-5 0.0 2.024127635883089 0.0 78-79 3.2822249874967744E-4 8.205562468741936E-5 0.0 2.2516473692351306 0.0 80-81 3.2822249874967744E-4 8.205562468741936E-5 0.0 2.5057120971735527 0.0 82-83 3.2822249874967744E-4 8.205562468741936E-5 0.0 2.771121015225011 0.0 84-85 3.2822249874967744E-4 8.205562468741936E-5 0.0 3.03156556798288 0.0 86-87 3.2822249874967744E-4 8.205562468741936E-5 0.0 3.3126265964434634 0.0 88 3.2822249874967744E-4 8.205562468741936E-5 0.0 3.54340804087683 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3190 0.0 56.31431 1 GTATCAA 6130 0.0 42.842934 1 ATCAACG 7265 0.0 35.579643 3 TCAACGC 7325 0.0 35.287483 4 CAACGCA 7450 0.0 34.632328 5 AACGCAG 7770 0.0 33.266514 6 TATCAAC 7845 0.0 32.986015 2 ACGCAGA 8705 0.0 29.639387 7 CGCAGAG 8780 0.0 29.173292 8 GCAGAGT 9975 0.0 25.395649 9 GTACATG 6595 0.0 24.951412 1 TGTCGCG 100 0.0017595825 23.49922 9 TACATGG 6690 0.0 23.321276 2 ACATGGG 6885 0.0 21.911676 3 AGAGTAC 9135 0.0 21.32551 10-11 CAGAGTA 9910 0.0 21.270233 10-11 GAGTACT 6895 0.0 21.130552 12-13 GTGGTAT 1700 0.0 20.52425 1 TGGTATC 1610 0.0 19.615501 2 ATACCGC 145 6.606102E-4 19.446833 6 >>END_MODULE