FastQCFastQC Report
Wed 25 May 2016
SRR1033049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502118
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38300.036.3295024
CAACGCA40000.034.903025
GTATCAA41200.034.8006861
ATCAACG40300.034.4099123
GGTATCA24500.033.3863031
AACGCAG44350.031.370036
GTACATG87300.029.4549481
TATCAAC50000.028.4865042
TACATGG89850.027.9376432
CGCAGAG51750.025.9059318
ACGCAGA53350.025.725597
ACATGGG93450.025.3523253
CATGGGG65300.021.1641434
ACACCGT6150.019.1065276
TATACTG21750.019.0191025
GCAGAGT67400.018.7055599
CTATACT19750.016.8988744
AGAGTAC54500.016.27750210-11
GAGTACA62100.016.1997151
CCTATAC14900.016.0897923
Produced by FastQC (version 0.11.5)