FastQCFastQC Report
Wed 25 May 2016
SRR1033049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502118
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45630.13029258294552037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38320.10941949985694371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.052.6197971
GTATCAA79250.044.20291
TCAACGC93050.037.1249474
ATCAACG94000.036.749223
CAACGCA95950.036.0508355
AACGCAG101500.034.172196
TATCAAC104300.033.119832
ACGCAGA119100.028.8856287
CGCAGAG120500.028.433028
GTACATG86950.025.4852851
GCAGAGT138600.024.3469039
TACATGG89700.023.5896972
ACATGGG92900.021.956483
AGAGTAC125300.020.83575610-11
GAGTACT91550.020.6877712-13
CAGAGTA134300.020.06936810-11
GTCGTAC2351.2260898E-720.0628851
ACACCGT5650.019.9638966
GTGGTAT22650.019.5668471
AGTACTT101100.018.50115812-13
Produced by FastQC (version 0.11.5)