FastQCFastQC Report
Wed 25 May 2016
SRR1033048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749356
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.034.3147161
GTATCAA33650.033.9486241
TCAACGC31900.033.889644
ATCAACG32650.033.2551273
CAACGCA32550.033.2122845
AACGCAG34950.031.195476
TATCAAC39350.028.070682
GTACATG69650.027.4034921
TACATGG71400.025.5424982
ACGCAGA42800.025.3645327
CGCAGAG43350.024.628368
ACATGGG74250.024.1189463
CATGGGG51750.020.6179434
GAGTACA52850.019.7473561
GCAGAGT53750.019.4280249
TCTGTCG6850.017.1651748
TACGACT2203.19514E-517.0921674
AGTACAT53900.017.0921672
GTACGAC2203.19514E-517.0921673
CGTACAC1952.4669585E-416.8730373
Produced by FastQC (version 0.11.5)