##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033048_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2749356 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19607027973096 33.0 31.0 34.0 28.0 34.0 2 31.262666238930134 33.0 31.0 34.0 28.0 34.0 3 31.313786574019517 34.0 31.0 34.0 28.0 34.0 4 34.70379572525348 37.0 35.0 37.0 32.0 37.0 5 34.75508846435311 37.0 35.0 37.0 32.0 37.0 6 34.81655958704511 37.0 35.0 37.0 32.0 37.0 7 34.676805404611116 37.0 35.0 37.0 32.0 37.0 8 34.67486858740738 37.0 35.0 37.0 32.0 37.0 9 36.24146782010042 39.0 37.0 39.0 32.0 39.0 10-11 36.24639479208949 39.0 37.0 39.0 32.0 39.0 12-13 36.214272724230696 39.0 37.0 39.0 32.0 39.0 14-15 37.548415519852654 40.0 38.0 41.0 32.0 41.0 16-17 37.55425197755402 40.0 38.0 41.0 32.0 41.0 18-19 37.55030232534455 40.0 38.0 41.0 32.0 41.0 20-21 37.554388736853284 40.0 38.0 41.0 32.0 41.0 22-23 37.52015617475511 40.0 38.0 41.0 32.0 41.0 24-25 37.52647274489007 40.0 38.0 41.0 32.0 41.0 26-27 37.42432573300802 40.0 38.0 41.0 31.0 41.0 28-29 37.43237670930938 40.0 38.0 41.0 31.0 41.0 30-31 37.58988068478582 40.0 38.0 41.0 31.5 41.0 32-33 37.56913000717259 40.0 38.0 41.0 31.5 41.0 34-35 37.526886660003285 40.0 38.0 41.0 31.0 41.0 36-37 37.44609246674494 40.0 38.0 41.0 31.0 41.0 38-39 37.34901736988589 40.0 38.0 41.0 30.5 41.0 40-41 37.27420003084359 40.0 38.0 41.0 30.0 41.0 42-43 37.15529345781339 40.0 38.0 41.0 30.0 41.0 44-45 37.022521819655225 40.0 37.5 41.0 30.0 41.0 46-47 36.88392063450495 40.0 37.0 41.0 30.0 41.0 48-49 36.76713019339802 40.0 37.0 41.0 30.0 41.0 50-51 36.64574449434704 40.0 37.0 41.0 30.0 41.0 52-53 36.48660031658323 40.0 36.0 41.0 30.0 41.0 54-55 36.32756998366163 40.0 36.0 41.0 29.5 41.0 56-57 36.1523256719028 40.0 35.5 41.0 29.0 41.0 58-59 35.95931610893605 39.0 35.0 41.0 29.0 41.0 60-61 35.74015132998419 39.0 35.0 41.0 28.0 41.0 62-63 35.48671688933699 39.0 35.0 41.0 28.0 41.0 64-65 35.209222814360885 38.0 35.0 40.0 28.0 41.0 66-67 34.91970992479693 38.0 34.0 40.0 28.0 41.0 68-69 34.60687230027686 37.0 34.0 40.0 27.0 41.0 70-71 34.27289008771508 37.0 34.0 39.5 26.5 41.0 72-73 33.920938030578796 36.0 34.0 39.0 26.0 41.0 74-75 33.56529692771689 36.0 34.0 39.0 26.0 40.5 76-77 33.194669224356545 35.0 33.0 38.5 26.0 40.0 78-79 32.8000962770918 35.0 33.0 37.0 25.5 39.0 80-81 32.4423790516761 35.0 33.0 37.0 25.0 39.0 82-83 32.207855221368206 35.0 33.0 36.5 25.0 39.0 84-85 31.96634684631601 35.0 33.0 36.0 25.0 37.0 86-87 31.777897987746947 35.0 33.0 36.0 25.0 37.0 88-89 31.54530697370584 35.0 33.0 35.0 24.0 37.0 90-91 31.320762025725298 35.0 33.0 35.0 23.5 36.0 92-93 31.08199683853237 35.0 32.0 35.0 22.5 36.0 94-95 30.861324251933908 35.0 32.0 35.0 20.0 36.0 96-97 30.669245270528805 35.0 32.0 35.0 18.5 35.5 98-99 30.474926128155104 35.0 32.0 35.0 16.5 35.0 100 30.32409116898648 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1118230670483058 1101 2 0.19420598461922722 1101 3 0.1304072828599523 1101 4 0.0018893426463293395 1101 5 0.08494231831761567 1101 6 0.19867946497583233 1101 7 0.2049317546753855 1101 8 0.26190538254103757 1101 9 0.32653145963647034 1101 10-11 0.2733085005270297 1101 12-13 0.28243407925501174 1101 14-15 0.42127831866208254 1101 16-17 0.39677172868673694 1101 18-19 0.3739135576403356 1101 20-21 0.4069004752833365 1101 22-23 0.3524316983743745 1101 24-25 0.2957974564528527 1101 26-27 0.19970831512532072 1101 28-29 0.20211715364352756 1101 30-31 0.2775526933667223 1101 32-33 0.26338672728065404 1101 34-35 0.2951203933491229 1101 36-37 0.23369097833079167 1101 38-39 0.3597737720830736 1101 40-41 0.38344888160120405 1101 42-43 0.38313639954728984 1101 44-45 0.4047269441970016 1101 46-47 0.35289088069077224 1101 48-49 0.3308898896416892 1101 50-51 0.39576836107890045 1101 52-53 0.31200759485003005 1101 54-55 0.33027210878282887 1101 56-57 0.42872618588949507 1101 58-59 0.42089643670782095 1101 60-61 0.4521534484128722 1101 62-63 0.42371951747925607 1101 64-65 0.3870100095269464 1101 66-67 0.4092888413750515 1101 68-69 0.37545384058066844 1101 70-71 0.34338460033671225 1101 72-73 0.2564133477308772 1101 74-75 0.1872836707396459 1101 76-77 0.19838717166297215 1101 78-79 0.22238427859462462 1101 80-81 0.17627665998261222 1101 82-83 0.2509097958143798 1101 84-85 0.1986154884284872 1101 86-87 0.14482673818103464 1101 88-89 0.2336981410173813 1101 90-91 0.2427720724762743 1101 92-93 0.3202295967035127 1101 94-95 0.36603476491223574 1101 96-97 0.21943944138789462 1101 98-99 0.21663794825606786 1101 100 0.1759446382950216 1102 1 0.14060873304098465 1102 2 0.17775756795444408 1102 3 0.241585141057719 1102 4 0.05760683355223506 1102 5 0.15733328111439704 1102 6 0.24294998265473566 1102 7 0.26794433542826823 1102 8 0.3260289707933097 1102 9 0.3548440988907444 1102 10-11 0.351285416188027 1102 12-13 0.31304299524693135 1102 14-15 0.4326861252174936 1102 16-17 0.402526743209755 1102 18-19 0.3103354202386015 1102 20-21 0.384083925343198 1102 22-23 0.3983528372359757 1102 24-25 0.3454438970707727 1102 26-27 0.22881787183642643 1102 28-29 0.32697264772226475 1102 30-31 0.3876613050332409 1102 32-33 0.3168521165541378 1102 34-35 0.2990624269634239 1102 36-37 0.3617447356144581 1102 38-39 0.3763092418100129 1102 40-41 0.22130412187125614 1102 42-43 0.3330935720350112 1102 44-45 0.27918079072446744 1102 46-47 0.27241306315139724 1102 48-49 0.25582910743533205 1102 50-51 0.24790711357104556 1102 52-53 0.2697350458369172 1102 54-55 0.3068514840963914 1102 56-57 0.27632322591242797 1102 58-59 0.2934982437506761 1102 60-61 0.22462589374059405 1102 62-63 0.24787870956918567 1102 64-65 0.23501514681063895 1102 66-67 0.33888316401647245 1102 68-69 0.3187983313108873 1102 70-71 0.27730801122557835 1102 72-73 0.2116925754722132 1102 74-75 0.22201038393741612 1102 76-77 0.20373880910368314 1102 78-79 0.15548472096542554 1102 80-81 0.2702053646777287 1102 82-83 0.15944423157002063 1102 84-85 0.22802306495627533 1102 86-87 0.23001968970789832 1102 88-89 0.14267011209328118 1102 90-91 0.16700095746059063 1102 92-93 0.2692018372979952 1102 94-95 0.31971734725196654 1102 96-97 0.25939733739609494 1102 98-99 0.29249096377270334 1102 100 0.2783640071977942 1103 1 0.11696198043731698 1103 2 0.19052820047082264 1103 3 0.09970213292951868 1103 4 0.12804332816350694 1103 5 0.17342670135495553 1103 6 0.231583323497901 1103 7 0.2592128092265611 1103 8 0.2424587685942825 1103 9 0.2549964464092582 1103 10-11 0.26730976631204584 1103 12-13 0.36804017940728073 1103 14-15 0.4499389416456552 1103 16-17 0.4761381368433675 1103 18-19 0.4185687636850517 1103 20-21 0.44903233669590037 1103 22-23 0.41047887995171095 1103 24-25 0.3745741287201483 1103 26-27 0.36639711795577057 1103 28-29 0.40448405197671633 1103 30-31 0.3986955261742082 1103 32-33 0.370536026886505 1103 34-35 0.2795914530983197 1103 36-37 0.39681533654524515 1103 38-39 0.3958415590224149 1103 40-41 0.38341790284116684 1103 42-43 0.35874949235346065 1103 44-45 0.4035261789717737 1103 46-47 0.4355129072728303 1103 48-49 0.4616661554106827 1103 50-51 0.3663524972903005 1103 52-53 0.4139074901560633 1103 54-55 0.4008475282120898 1103 56-57 0.42225094849426625 1103 58-59 0.39284801811655257 1103 60-61 0.432105953344724 1103 62-63 0.35250522343783786 1103 64-65 0.3871271234829976 1103 66-67 0.34541131472780506 1103 68-69 0.32657879330244555 1103 70-71 0.40523305380935426 1103 72-73 0.34179689931966806 1103 74-75 0.3138848898499873 1103 76-77 0.2862831719062484 1103 78-79 0.2902268372721366 1103 80-81 0.2892629633113941 1103 82-83 0.33883367694168953 1103 84-85 0.2534908010558041 1103 86-87 0.20349755424489402 1103 88-89 0.21467647019968084 1103 90-91 0.25007251994487945 1103 92-93 0.29929133765712734 1103 94-95 0.28209275829047087 1103 96-97 0.32183132925418434 1103 98-99 0.2974169522625836 1103 100 0.28070170107842785 1104 1 0.11039555170564697 1104 2 0.17866844173390106 1104 3 0.1252696947968488 1104 4 0.12018086139060813 1104 5 0.13058031670400538 1104 6 0.14675194208970055 1104 7 0.23315713863458853 1104 8 0.2342238205415299 1104 9 0.30411856215611976 1104 10-11 0.2728830556940878 1104 12-13 0.35568356840978765 1104 14-15 0.4404778705937602 1104 16-17 0.31838254068116356 1104 18-19 0.32033128100422914 1104 20-21 0.3192726117229938 1104 22-23 0.39166937320815265 1104 24-25 0.31790574304550034 1104 26-27 0.2589857046027504 1104 28-29 0.2863262677895264 1104 30-31 0.24618628428996914 1104 32-33 0.27947958034727094 1104 34-35 0.28134082519275694 1104 36-37 0.3042442303210606 1104 38-39 0.36125156569676165 1104 40-41 0.3938947201036669 1104 42-43 0.32193096121696385 1104 44-45 0.3578770422413555 1104 46-47 0.2813538632185697 1104 48-49 0.314625612931124 1104 50-51 0.32188556640704746 1104 52-53 0.32999441273859986 1104 54-55 0.3437695926546738 1104 56-57 0.42993019995716253 1104 58-59 0.3603655161593906 1104 60-61 0.3856444656616702 1104 62-63 0.3755830385139802 1104 64-65 0.3581256791705414 1104 66-67 0.3284742544894286 1104 68-69 0.35497397479631587 1104 70-71 0.3490752839841633 1104 72-73 0.34288591835611015 1104 74-75 0.3747350793686266 1104 76-77 0.3354451156228748 1104 78-79 0.4067335559647276 1104 80-81 0.3422367894867122 1104 82-83 0.3737113758185657 1104 84-85 0.41520971239980753 1104 86-87 0.3339089552306298 1104 88-89 0.28757904220885067 1104 90-91 0.40367769590538316 1104 92-93 0.4230430898550779 1104 94-95 0.4144450580265975 1104 96-97 0.4382505460966719 1104 98-99 0.4703297505518016 1104 100 0.5392303720968705 1105 1 0.2243805425964993 1105 2 0.22290950854275238 1105 3 0.26982977595859126 1105 4 0.28647185349467463 1105 5 0.24597596641261532 1105 6 0.3812648250628996 1105 7 0.3364149837642998 1105 8 0.39520980841486875 1105 9 0.39139236501917907 1105 10-11 0.5018336082188313 1105 12-13 0.49202380578633154 1105 14-15 0.5577236632349312 1105 16-17 0.5019275438294599 1105 18-19 0.582416906240546 1105 20-21 0.533012666640964 1105 22-23 0.49543241209297406 1105 24-25 0.451022261709916 1105 26-27 0.4809827489843812 1105 28-29 0.4527533145539948 1105 30-31 0.47805324237587143 1105 32-33 0.5000726684197403 1105 34-35 0.4973401266285009 1105 36-37 0.5225161832917422 1105 38-39 0.5932369765279617 1105 40-41 0.6341132544567074 1105 42-43 0.5714327831717796 1105 44-45 0.47746827440798256 1105 46-47 0.5076471529676425 1105 48-49 0.5103398660724707 1105 50-51 0.5044164862693492 1105 52-53 0.4621831664052536 1105 54-55 0.5348626853625191 1105 56-57 0.4821135515271635 1105 58-59 0.4319516278600162 1105 60-61 0.46652094331425786 1105 62-63 0.4142245039153778 1105 64-65 0.5040543248904754 1105 66-67 0.4303649757870929 1105 68-69 0.42063293913220434 1105 70-71 0.28124307821740047 1105 72-73 0.34652293755134167 1105 74-75 0.28426681079822913 1105 76-77 0.3152676833075674 1105 78-79 0.4314189577506724 1105 80-81 0.45925588700081477 1105 82-83 0.472971080612524 1105 84-85 0.48349474465829445 1105 86-87 0.4507203376186375 1105 88-89 0.4053391114438831 1105 90-91 0.48900207101234017 1105 92-93 0.5579401581151799 1105 94-95 0.5108631497384657 1105 96-97 0.6606724167705771 1105 98-99 0.5546780023746969 1105 100 0.5613022966539347 1106 1 0.10538952274850999 1106 2 0.14932836617527911 1106 3 0.18004590510238216 1106 4 0.17506922533556946 1106 5 0.0777839754104761 1106 6 0.1549555614025948 1106 7 0.2005544795308296 1106 8 0.1807451750294149 1106 9 0.1588659294038024 1106 10-11 0.15121111397471765 1106 12-13 0.2652365668761689 1106 14-15 0.2588340536518601 1106 16-17 0.30901426329768356 1106 18-19 0.33043868504576324 1106 20-21 0.3170028208929452 1106 22-23 0.3294075132869949 1106 24-25 0.28504413641137916 1106 26-27 0.3069551831548125 1106 28-29 0.33232351993216014 1106 30-31 0.3086696864833627 1106 32-33 0.28630057207872284 1106 34-35 0.3142641007948299 1106 36-37 0.2811366039605616 1106 38-39 0.23642956688154726 1106 40-41 0.3059788180597067 1106 42-43 0.317728399689976 1106 44-45 0.2645935788574434 1106 46-47 0.21488086714314392 1106 48-49 0.2909164578264267 1106 50-51 0.2758010146171159 1106 52-53 0.2055241911143071 1106 54-55 0.19631383990616058 1106 56-57 0.24677308557026834 1106 58-59 0.31276188837128416 1106 60-61 0.2897413604411838 1106 62-63 0.23602060298679106 1106 64-65 0.35934790530036764 1106 66-67 0.28310260839585766 1106 68-69 0.3176146654788141 1106 70-71 0.32230368210585425 1106 72-73 0.3743814253135511 1106 74-75 0.4945133954286405 1106 76-77 0.41807109398474296 1106 78-79 0.41992580644028266 1106 80-81 0.43263402090111214 1106 82-83 0.43405913660436113 1106 84-85 0.3905840663358191 1106 86-87 0.3397355387374965 1106 88-89 0.3482228761757291 1106 90-91 0.30392128988211553 1106 92-93 0.3221286742041194 1106 94-95 0.3729043130754057 1106 96-97 0.2664233414885402 1106 98-99 0.29137432780217765 1106 100 0.27710532097531626 1107 1 0.2555594238480765 1107 2 0.2155805231824388 1107 3 0.23357456099881446 1107 4 0.19816443941734718 1107 5 0.17468425039709956 1107 6 0.22243380821972636 1107 7 0.3199959843450557 1107 8 0.33765136189418143 1107 9 0.35853069045376884 1107 10-11 0.29259277860729327 1107 12-13 0.33925020023683317 1107 14-15 0.3602398831589966 1107 16-17 0.3324820070849128 1107 18-19 0.27503819599334633 1107 20-21 0.2989057015820009 1107 22-23 0.313783180880165 1107 24-25 0.29596207320659573 1107 26-27 0.3671703650555145 1107 28-29 0.30348866856764545 1107 30-31 0.3699469137485707 1107 32-33 0.2946021104168395 1107 34-35 0.3491888551423372 1107 36-37 0.36671228980672765 1107 38-39 0.3870158862607411 1107 40-41 0.385631987875648 1107 42-43 0.3900615366388962 1107 44-45 0.33541555372120513 1107 46-47 0.292543123347194 1107 48-49 0.365276694661965 1107 50-51 0.4030754673106074 1107 52-53 0.43531901057676947 1107 54-55 0.38072447303227364 1107 56-57 0.4302139846640429 1107 58-59 0.4440658644145259 1107 60-61 0.4855338413111383 1107 62-63 0.47998872883712806 1107 64-65 0.435127632325063 1107 66-67 0.4797852134937344 1107 68-69 0.459419229948665 1107 70-71 0.46317946552416345 1107 72-73 0.47977306398792763 1107 74-75 0.4866734718819572 1107 76-77 0.48224139211604466 1107 78-79 0.4259080806880604 1107 80-81 0.4598051755862471 1107 82-83 0.4732035012387712 1107 84-85 0.528813484992412 1107 86-87 0.4449501117205621 1107 88-89 0.4489784281530298 1107 90-91 0.43609734061382 1107 92-93 0.5114147175503376 1107 94-95 0.5068508172632811 1107 96-97 0.5284213005245171 1107 98-99 0.42062565557687037 1107 100 0.4132568626995443 1108 1 -0.07073757646367085 1108 2 0.016882972205948477 1108 3 0.010650520178995038 1108 4 0.028301746953673046 1108 5 0.00439890343140803 1108 6 0.028178543660629884 1108 7 0.030432129581498657 1108 8 -0.04650047370094512 1108 9 0.07096809923957181 1108 10-11 0.15154218421491095 1108 12-13 0.06829307539098295 1108 14-15 0.13495165320404112 1108 16-17 0.0815522775341293 1108 18-19 0.10129891754073128 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2215 14-15 -0.5525403211788813 2215 16-17 -0.5020514737030339 2215 18-19 -0.4649704279629532 2215 20-21 -0.4584496659992823 2215 22-23 -0.5249190206044432 2215 24-25 -0.4397211496347708 2215 26-27 -0.4081135662331761 2215 28-29 -0.4557130096590001 2215 30-31 -0.5458826787754347 2215 32-33 -0.555967890331516 2215 34-35 -0.5390654589145925 2215 36-37 -0.49674161657226534 2215 38-39 -0.4653774991885342 2215 40-41 -0.42683117322010133 2215 42-43 -0.5233796001078304 2215 44-45 -0.43905005201669667 2215 46-47 -0.4330207301595763 2215 48-49 -0.3542841250177631 2215 50-51 -0.37123999130925256 2215 52-53 -0.3784295476426536 2215 54-55 -0.3341691945367842 2215 56-57 -0.4518138566979317 2215 58-59 -0.39504650953732323 2215 60-61 -0.555350482450713 2215 62-63 -0.5661705815314519 2215 64-65 -0.7427490688572291 2215 66-67 -0.6562755153137303 2215 68-69 -0.5728222503308729 2215 70-71 -0.5979168697695485 2215 72-73 -0.6174844941495365 2215 74-75 -0.5751042272322451 2215 76-77 -0.49563590300262916 2215 78-79 -0.5761341101769588 2215 80-81 -0.6431466882455226 2215 82-83 -0.5703447459955626 2215 84-85 -0.5582236158377896 2215 86-87 -0.5490571889454756 2215 88-89 -0.5081104995661043 2215 90-91 -0.56000168521172 2215 92-93 -0.6020115707867717 2215 94-95 -0.7212226550432526 2215 96-97 -0.6862939485545887 2215 98-99 -0.5683840267740727 2215 100 -0.4893584767766761 2216 1 -0.4872128142179015 2216 2 -0.5704786878067054 2216 3 -0.49733909706237256 2216 4 -0.5915986684142638 2216 5 -0.5192788931506129 2216 6 -0.5722212731819738 2216 7 -0.5153542424718296 2216 8 -0.45385086396633767 2216 9 -0.5559762921412812 2216 10-11 -0.6149357413836256 2216 12-13 -0.6878190825928243 2216 14-15 -0.8164237071350868 2216 16-17 -0.8244480469270812 2216 18-19 -0.7312770836572895 2216 20-21 -0.6452938520159961 2216 22-23 -0.6936280319075792 2216 24-25 -0.7343114992933906 2216 26-27 -0.751531856672571 2216 28-29 -0.726064358765008 2216 30-31 -0.9137218524275994 2216 32-33 -0.9370477124539534 2216 34-35 -1.1489035314750424 2216 36-37 -1.1614676848771524 2216 38-39 -1.0807234275671505 2216 40-41 -0.971767639319161 2216 42-43 -1.06652621352751 2216 44-45 -0.9798098094293266 2216 46-47 -0.8435804848442885 2216 48-49 -0.9176077686889386 2216 50-51 -0.8588646546062364 2216 52-53 -0.9887495544872849 2216 54-55 -1.0378582030871897 2216 56-57 -1.145806789706164 2216 58-59 -1.0293524807692407 2216 60-61 -1.0004729024386734 2216 62-63 -1.0822553242607995 2216 64-65 -1.1149437752679034 2216 66-67 -1.117324168770324 2216 68-69 -1.084861349006296 2216 70-71 -1.0995059716068951 2216 72-73 -1.027572792280857 2216 74-75 -1.0874551365628804 2216 76-77 -0.9184220122181941 2216 78-79 -1.0565973788073784 2216 80-81 -0.9864111813031471 2216 82-83 -1.2027725788164432 2216 84-85 -0.9745720171261993 2216 86-87 -0.9767825665881098 2216 88-89 -1.0808139855712007 2216 90-91 -1.0691930937204681 2216 92-93 -1.2396201144979209 2216 94-95 -1.139760778070407 2216 96-97 -1.0518745822361737 2216 98-99 -0.9998634286299755 2216 100 -0.9756723284194457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47038.0 3 8733.0 4 4788.0 5 3856.0 6 3322.0 7 3584.0 8 4016.0 9 4534.0 10 5093.0 11 5752.0 12 6267.0 13 6979.0 14 7412.0 15 7497.0 16 7588.0 17 7738.0 18 7647.0 19 7512.0 20 7419.0 21 7887.0 22 9056.0 23 12611.0 24 18935.0 25 23171.0 26 23041.0 27 21098.0 28 23765.0 29 29147.0 30 35449.0 31 42943.0 32 55156.0 33 73762.0 34 103521.0 35 155806.0 36 273463.0 37 543575.0 38 822965.0 39 316800.0 40 430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66000020380801 20.194352771279377 13.451219281402082 23.694427743510534 2 15.219058008535772 20.328076678581137 40.84649619623615 23.60636911664694 3 17.770281606231663 25.853118143310965 30.23368378683392 26.14291646362345 4 12.008143211618309 17.021903899765555 40.00190731400322 30.968045574612923 5 12.439770395793502 38.44188616768621 36.18111910208563 12.93722433443466 6 30.251871094945944 39.005198911799795 18.032735561015407 12.710194432238858 7 26.55663306949249 33.01077085164213 23.097590046518604 17.335006032346776 8 24.459479238803457 37.178602142785884 20.80161134935216 17.560307269058494 9 24.54308407689877 17.388690912215345 21.499623544968536 36.56860146591735 10-11 23.240575194277984 27.514863833828716 29.414780291609492 19.829780680283797 12-13 23.91740370231267 24.94567015187234 29.24923859928849 21.887687546526504 14-15 21.30878427508694 27.00349072737991 26.184333126154456 25.503391871378696 16-17 20.988987409111864 30.281722054793047 26.73748741702883 21.991803119066255 18-19 20.903130369659195 29.263337598568718 28.67579823889287 21.157733792879224 20-21 22.23059143212148 28.539365765983543 28.13573847193922 21.094304329955758 22-23 21.84603402925814 28.313649279009528 28.169605854903 21.670710836829336 24-25 21.683595929264357 28.419423943648393 28.16347044638428 21.73350968070297 26-27 21.449453543449973 28.227715675703646 28.32930743787981 21.993523342966576 28-29 21.512564364102232 28.27272636282655 28.36806576034489 21.846643512726327 30-31 21.75901540943494 28.814311085452783 27.93363674385641 21.493036761255862 32-33 21.34589408449843 28.421954173144144 28.502704979105253 21.729446763252177 34-35 21.51805736038615 28.129170007523296 28.56632503830768 21.786447593782878 36-37 21.893399822810373 28.561992806515953 27.89755899361095 21.64704837706273 38-39 21.6906376878909 28.715265618959773 28.099221536353664 21.49487515679567 40-41 21.406774416657566 28.403152415274292 28.3986203632922 21.791452804775947 42-43 21.37656741031429 28.30151935765502 28.432013654399054 21.88989957763164 44-45 21.585389246752925 28.442565279095728 28.327016265675738 21.64502920847561 46-47 21.589371959574073 28.67501132083164 28.1615333429433 21.574083376650986 48-49 21.49018140414584 28.47665799370713 28.267684319803614 21.765476282343418 50-51 21.549628078778287 28.34232258214807 28.223681241164233 21.884368097909405 52-53 21.556215119893654 28.283443770320186 28.3066668413857 21.85367426840046 54-55 21.696595362515595 28.781447878573356 28.016061859816322 21.505894899094724 56-57 21.53428953447298 28.394125501445078 28.111116024786032 21.960468939295914 58-59 21.346207495964084 28.025489182329437 28.552437408519726 22.075865913186753 60-61 21.68395660140548 28.59174759940537 28.025926622156316 21.698369177032834 62-63 21.536613365596423 28.49516199244962 28.098152006324494 21.87007263562946 64-65 21.497785379739266 28.181281668141622 28.302390159330002 22.018542792789102 66-67 21.33936625891704 28.27861983269387 28.154105664521122 22.22790824386797 68-69 21.848835228637608 28.409709655205546 28.023666674916615 21.71778844124023 70-71 21.690381353565048 28.188417255962673 28.193457836273584 21.927743554198702 72-73 21.471252044184038 28.363821177046034 28.295437568658837 21.86948921011109 74-75 21.565533201014897 28.36014003893125 28.148887900979176 21.92543885907468 76-77 21.5831648959206 28.35929829107295 28.025359141074734 22.032177671931716 78-79 21.676867689716648 28.462037019178453 28.101310551553954 21.75978473955094 80-81 21.595973776638562 28.193315779284767 28.222554332400318 21.988156111676354 82-83 21.44599898140639 28.31080679055128 28.380077706428512 21.86311652161382 84-85 21.465331900393586 28.189778278227195 28.225347059426063 22.119542761953156 86-87 21.621015340984965 28.325696377233733 28.144290621808203 21.908997659973096 88-89 21.36059419494074 28.190037148100078 28.26918017647258 22.180188480486603 90-91 21.363759911130483 28.07653024652703 28.369402310301755 22.19030753204073 92-93 21.537152158753024 28.293965709058433 28.135681351391824 22.033200780796726 94-95 21.27706373947856 28.024102544976227 28.354220810822856 22.344612904722354 96-97 21.338534857208565 27.99045473549844 28.425526232193143 22.245484175099858 98-99 21.331085777080748 28.09444102802956 28.302888831476945 22.271584363412742 100 21.430432573826643 27.993568963333775 28.210969444569788 22.36502901826979 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 370.0 1 328.5 2 278.0 3 274.5 4 289.0 5 302.0 6 295.5 7 262.5 8 243.5 9 230.5 10 216.5 11 219.5 12 225.5 13 239.5 14 275.5 15 319.5 16 417.0 17 568.0 18 774.5 19 1120.5 20 1638.0 21 2485.5 22 3856.5 23 5864.0 24 8502.5 25 11966.0 26 16480.5 27 22318.5 28 30162.0 29 39117.0 30 47646.5 31 56340.0 32 65376.5 33 74054.5 34 82192.5 35 88545.0 36 93290.0 37 98255.0 38 102716.0 39 104599.0 40 104244.0 41 103899.0 42 102812.0 43 100873.0 44 98483.0 45 95399.5 46 93195.0 47 90618.5 48 87829.0 49 85634.0 50 83217.5 51 79629.5 52 74006.0 53 69596.5 54 66690.5 55 63313.5 56 58270.0 57 52281.0 58 47145.0 59 42376.0 60 36981.5 61 31149.0 62 26292.0 63 21735.5 64 17436.0 65 13802.5 66 10596.0 67 7869.0 68 5984.0 69 4606.0 70 3253.0 71 2275.5 72 1681.0 73 1184.0 74 822.0 75 562.0 76 365.5 77 249.5 78 157.5 79 97.5 80 71.0 81 48.0 82 31.0 83 23.5 84 13.5 85 8.5 86 8.5 87 11.5 88 11.5 89 10.5 90 7.0 91 1.5 92 1.5 93 3.5 94 3.0 95 2.0 96 2.0 97 1.5 98 0.5 99 0.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06081424158966681 2 0.04208258224835198 3 0.05757711987825513 4 0.07409007782186083 5 0.08612926081598746 6 0.08856619513806142 7 0.09085764084389217 8 0.10322417322456605 9 0.09747737288295877 10-11 0.10331510360971806 12-13 0.10684320255361619 14-15 0.10682501647658578 16-17 0.07607236021817473 18-19 0.09935053881709026 20-21 0.09147596746292586 22-23 0.057231584414677475 24-25 0.022350688670364986 26-27 0.18327928431239898 28-29 0.06559717984866274 30-31 0.07794552615230622 32-33 0.07328989043252311 34-35 0.020368406274051086 36-37 0.05283055377332001 38-39 0.07820013123073186 40-41 0.08205557956117723 42-43 0.07672705899126923 44-45 0.07409007782186083 46-47 0.08030971616625857 48-49 0.07710896660890769 50-51 0.07274430812161102 52-53 0.07594505767896191 54-55 0.06741578755170302 56-57 0.06988909402783779 58-59 0.06683383308673013 60-61 0.06385131645374408 62-63 0.07070746749420592 64-65 0.06617913431363562 66-67 0.06828871924916236 68-69 0.06779769516934148 70-71 0.06025047320172433 72-73 0.05897744780959614 74-75 0.061523498593852526 76-77 0.05637683879424854 78-79 0.05197580815289107 80-81 0.04626537996534461 82-83 0.05272143731113759 84-85 0.042173512633503996 86-87 0.039900253004703644 88-89 0.042446303788960034 90-91 0.03844536684227143 92-93 0.041373325244166266 94-95 0.039954811235794856 96-97 0.043173746870176145 98-99 0.04313737471611534 100 0.0520849246150735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2749356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.1623179965308 #Duplication Level Percentage of deduplicated Percentage of total 1 79.69518001001806 55.119033826436336 2 12.787408179777652 17.68813581762442 3 3.675412506681409 7.62600145666578 4 1.5111445841802702 4.180570490792446 5 0.7460053222310264 2.579772866162334 6 0.41864674137001584 1.7372747432906657 7 0.2563214905784748 1.2409451908513274 8 0.18332081918378054 1.0143114233418524 9 0.13081129627566468 0.8142491223500333 >10 0.5713960660902351 6.675255402897812 >50 0.020587980894862773 0.9443297888077882 >100 0.0037123903375417358 0.35817794157637184 >500 5.261238105863196E-5 0.0219419292028705 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.274430812161102E-5 0.0 5 0.0 0.0 0.0 7.274430812161102E-5 0.0 6 0.0 0.0 0.0 1.0911646218241654E-4 0.0 7 0.0 0.0 0.0 1.8186077030402757E-4 0.0 8 0.0 0.0 0.0 1.8186077030402757E-4 0.0 9 0.0 0.0 0.0 1.8186077030402757E-4 0.0 10-11 0.0 0.0 0.0 1.8186077030402757E-4 0.0 12-13 0.0 0.0 0.0 1.8186077030402757E-4 0.0 14-15 0.0 0.0 0.0 2.1823292436483308E-4 0.0 16-17 0.0 0.0 0.0 2.1823292436483308E-4 0.0 18-19 0.0 0.0 0.0 2.1823292436483308E-4 0.0 20-21 0.0 0.0 0.0 3.455354635776524E-4 0.0 22-23 0.0 0.0 0.0 4.3646584872966617E-4 0.0 24-25 0.0 0.0 0.0 5.455823109120827E-4 0.0 26-27 0.0 0.0 0.0 6.365126960640964E-4 0.0 28-29 0.0 0.0 0.0 0.0011820950069761792 0.0 30-31 0.0 0.0 0.0 0.0034007964046853154 0.0 32-33 0.0 0.0 0.0 0.009656806903143864 0.0 34-35 0.0 0.0 0.0 0.01814970487634195 0.0 36-37 0.0 0.0 0.0 0.032189356343812876 0.0 38-39 0.0 0.0 0.0 0.05584944256036686 0.0 40-41 0.0 0.0 0.0 0.08572916712131859 0.0 42-43 0.0 0.0 0.0 0.11582712460663516 0.0 44-45 0.0 0.0 0.0 0.15227202297556228 0.0 46-47 0.0 0.0 0.0 0.19757354085829554 0.0 48-49 0.0 0.0 0.0 0.250876932634406 0.0 50-51 0.0 0.0 0.0 0.30801758666393153 0.0 52-53 0.0 0.0 0.0 0.3686863396373551 0.0 54-55 0.0 0.0 0.0 0.4339561700994706 0.0 56-57 0.0 0.0 0.0 0.5100467163946757 0.0 58-59 0.0 0.0 0.0 0.6006315660831119 0.0 60-61 0.0 0.0 0.0 0.6984181022755875 0.0 62-63 0.0 0.0 0.0 0.8012058096514239 0.0 64-65 0.0 0.0 0.0 0.9073943134319455 0.0 66-67 0.0 0.0 0.0 1.0303321941574681 0.0 68-69 0.0 0.0 0.0 1.1680735415857386 0.0 70-71 0.0 0.0 0.0 1.325183061051388 0.0 72-73 0.0 0.0 0.0 1.4887304517858 0.0 74-75 0.0 0.0 0.0 1.6529689134473673 0.0 76-77 0.0 0.0 0.0 1.8288100922543316 0.0 78-79 0.0 0.0 0.0 2.033167039844967 0.0 80-81 0.0 0.0 0.0 2.2667671993004905 0.0 82-83 0.0 0.0 0.0 2.5126247746745056 0.0 84-85 0.0 0.0 0.0 2.7594643982081624 0.0 86-87 0.0 0.0 0.0 3.0181613439656414 0.0 88 0.0 0.0 0.0 3.2300291413698337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2055 0.0 34.314716 1 GTATCAA 3365 0.0 33.948624 1 TCAACGC 3190 0.0 33.88964 4 ATCAACG 3265 0.0 33.255127 3 CAACGCA 3255 0.0 33.212284 5 AACGCAG 3495 0.0 31.19547 6 TATCAAC 3935 0.0 28.07068 2 GTACATG 6965 0.0 27.403492 1 TACATGG 7140 0.0 25.542498 2 ACGCAGA 4280 0.0 25.364532 7 CGCAGAG 4335 0.0 24.62836 8 ACATGGG 7425 0.0 24.118946 3 CATGGGG 5175 0.0 20.617943 4 GAGTACA 5285 0.0 19.747356 1 GCAGAGT 5375 0.0 19.428024 9 TCTGTCG 685 0.0 17.165174 8 TACGACT 220 3.19514E-5 17.092167 4 AGTACAT 5390 0.0 17.092167 2 GTACGAC 220 3.19514E-5 17.092167 3 CGTACAC 195 2.4669585E-4 16.873037 3 >>END_MODULE