FastQCFastQC Report
Wed 25 May 2016
SRR1033048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749356
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.15618202953709887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.13166719770011595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36000.056.0395281
GTATCAA68400.042.1743621
ATCAACG81450.034.3331383
TCAACGC81450.034.274194
CAACGCA83450.033.3970455
AACGCAG85150.032.7854776
TATCAAC87800.032.0328372
ACGCAGA97800.028.35267
CGCAGAG99150.027.7295518
GTACATG65450.025.0624081
GCAGAGT111900.024.2760189
GTGGTAT19550.024.1105141
TACATGG67450.023.0487632
TGGTATC19000.022.3153932
ACATGGG69500.021.7074033
GAGTACT79250.021.28996312-13
AGAGTAC101750.020.85503410-11
CAGAGTA110850.020.79653210-11
CATGGGG48550.019.4571234
GAGTACA50200.018.87321
Produced by FastQC (version 0.11.5)