FastQCFastQC Report
Wed 25 May 2016
SRR1033047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888828
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19550.031.7418864
ATCAACG20300.030.5691533
CAACGCA20350.030.495665
GTATCAA22200.030.2830831
AACGCAG21800.029.1057766
GGTATCA14850.026.2765711
GTACATG45750.025.998391
TACATGG45950.025.066062
TATCAAC26650.023.8145332
ACGCAGA26700.022.884117
ACATGGG48900.022.592513
CGCAGAG27100.022.2189048
GTGTAGG6950.021.6462291
GTGTACG2057.5454045E-720.6398091
ACGCGTT805.3545293E-520.59353310-11
CTAGTAC4400.020.3004863
AGTACCG2351.2625605E-720.006025
GAGTACA32450.019.9931581
CATGGGG34050.019.6054484
GTCTACG2552.9833063E-718.436431
Produced by FastQC (version 0.11.5)