##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1888828 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.988364213152284 31.0 31.0 34.0 28.0 34.0 2 31.01894931671915 33.0 31.0 34.0 28.0 34.0 3 31.237931669797355 34.0 31.0 34.0 28.0 34.0 4 34.66683678979769 37.0 35.0 37.0 32.0 37.0 5 34.692077838744446 37.0 35.0 37.0 32.0 37.0 6 34.7176074264041 37.0 35.0 37.0 32.0 37.0 7 34.67597473142075 37.0 35.0 37.0 32.0 37.0 8 34.651114871232316 37.0 35.0 37.0 32.0 37.0 9 36.23565830239704 39.0 37.0 39.0 32.0 39.0 10-11 36.222503319518765 39.0 37.0 39.0 32.0 39.0 12-13 36.1359078222051 39.0 37.0 39.0 32.0 39.0 14-15 37.44192430438346 40.0 38.0 41.0 32.0 41.0 16-17 37.427344363806554 40.0 38.0 41.0 32.0 41.0 18-19 37.415591043758354 40.0 38.0 41.0 32.0 41.0 20-21 37.4374606899093 40.0 38.0 41.0 31.5 41.0 22-23 37.379186723195545 40.0 38.0 41.0 31.0 41.0 24-25 37.37869594266921 40.0 38.0 41.0 31.0 41.0 26-27 37.27821882140671 40.0 38.0 41.0 31.0 41.0 28-29 37.291010086678085 40.0 38.0 41.0 31.0 41.0 30-31 37.438268863019815 40.0 38.0 41.0 31.0 41.0 32-33 37.430545555233195 40.0 38.0 41.0 31.0 41.0 34-35 37.38205252145775 40.0 38.0 41.0 31.0 41.0 36-37 37.2965624715432 40.0 38.0 41.0 30.0 41.0 38-39 37.19855063563226 40.0 38.0 41.0 30.0 41.0 40-41 37.12136838293375 40.0 38.0 41.0 30.0 41.0 42-43 36.99388218514338 40.0 37.5 41.0 30.0 41.0 44-45 36.83344539576923 40.0 37.0 41.0 30.0 41.0 46-47 36.690166335950124 40.0 37.0 41.0 30.0 41.0 48-49 36.565879211871064 40.0 37.0 41.0 30.0 41.0 50-51 36.440074215333524 40.0 36.0 41.0 29.5 41.0 52-53 36.281491750439955 40.0 36.0 41.0 29.0 41.0 54-55 36.12268348414996 40.0 36.0 41.0 29.0 41.0 56-57 35.950916653077996 39.5 35.0 41.0 28.5 41.0 58-59 35.75553359014161 39.0 35.0 41.0 28.0 41.0 60-61 35.53280499865525 39.0 35.0 41.0 28.0 41.0 62-63 35.28319254055954 39.0 35.0 40.5 28.0 41.0 64-65 35.01134883642132 38.0 34.5 40.0 27.0 41.0 66-67 34.72185609277287 38.0 34.0 40.0 27.0 41.0 68-69 34.41069303292835 37.0 34.0 40.0 26.0 41.0 70-71 34.075731088272725 37.0 34.0 39.5 26.0 41.0 72-73 33.72303168949211 36.0 34.0 39.0 26.0 41.0 74-75 33.365525076926005 36.0 33.0 39.0 26.0 40.5 76-77 33.002498904082316 35.0 33.0 38.5 25.0 40.0 78-79 32.608914628542145 35.0 33.0 37.0 24.5 39.0 80-81 32.246603978763545 35.0 33.0 37.0 24.0 39.0 82-83 32.01131839426353 35.0 33.0 36.5 24.0 39.0 84-85 31.772444341146997 35.0 32.5 36.0 24.0 37.0 86-87 31.581893110436738 35.0 33.0 36.0 23.5 37.0 88-89 31.346757619010305 35.0 32.5 35.0 23.0 37.0 90-91 31.118509467246355 35.0 32.0 35.0 21.0 36.0 92-93 30.8781458661138 35.0 32.0 35.0 20.0 36.0 94-95 30.655972910185575 35.0 32.0 35.0 18.5 36.0 96-97 30.456028288441296 35.0 32.0 35.0 13.5 35.5 98-99 30.255155048527445 35.0 32.0 35.0 2.0 35.0 100 30.09376608140074 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15882554984614217 1101 2 0.22169965506172318 1101 3 0.32182060345857977 1101 4 0.2221437133862807 1101 5 0.2590715666169032 1101 6 0.28406120710011606 1101 7 0.3743403231085978 1101 8 0.3716937965151317 1101 9 0.47456423477180465 1101 10-11 0.4713656688392689 1101 12-13 0.4509391943985719 1101 14-15 0.5675310261575177 1101 16-17 0.4940090500410719 1101 18-19 0.5857494641450387 1101 20-21 0.5454103655764797 1101 22-23 0.6213906955397164 1101 24-25 0.5255954241408602 1101 26-27 0.4790579787753728 1101 28-29 0.43262032945911955 1101 30-31 0.5231790245947749 1101 32-33 0.5832856441540528 1101 34-35 0.5660945869894789 1101 36-37 0.5553937665573443 1101 38-39 0.5296163564456435 1101 40-41 0.5900199138494955 1101 42-43 0.6758740188141417 1101 44-45 0.6027528773023789 1101 46-47 0.5876826034367681 1101 48-49 0.662714766989879 1101 50-51 0.5905511525336777 1101 52-53 0.5565748487603486 1101 54-55 0.5278568927886127 1101 56-57 0.6074720515990819 1101 58-59 0.6967221478448025 1101 60-61 0.7306001665960054 1101 62-63 0.6591825576859804 1101 64-65 0.6705222276404115 1101 66-67 0.5512240915261302 1101 68-69 0.6347967537502299 1101 70-71 0.5911155562183126 1101 72-73 0.5501045311341883 1101 74-75 0.6092719376458007 1101 76-77 0.64244030313629 1101 78-79 0.6329400980398603 1101 80-81 0.5884593683983894 1101 82-83 0.6356445665061159 1101 84-85 0.5472285246643018 1101 86-87 0.583916169688397 1101 88-89 0.5792220123036813 1101 90-91 0.5509213240258397 1101 92-93 0.5707121785658131 1101 94-95 0.5004901116951856 1101 96-97 0.4335835283207956 1101 98-99 0.4793185449933368 1101 100 0.5155282670576042 1102 1 0.031822633389165134 1102 2 0.07172783861117793 1102 3 0.1713568912334047 1102 4 0.018926742732638502 1102 5 0.020748202371265734 1102 6 0.03136575681734399 1102 7 0.12139878637631085 1102 8 0.12386063204291986 1102 9 0.11501156340362684 1102 10-11 0.17827310486233472 1102 12-13 0.17855717456090048 1102 14-15 0.3256222959635977 1102 16-17 0.26356592538609647 1102 18-19 0.21587425602462673 1102 20-21 0.35705612967490197 1102 22-23 0.2537174484622682 1102 24-25 0.24693591356468403 1102 26-27 0.01942959702656566 1102 28-29 0.06724146440539158 1102 30-31 0.21705752716980697 1102 32-33 0.23152993650032272 1102 34-35 0.3406695613277577 1102 36-37 0.3885836649302945 1102 38-39 0.3545699796806332 1102 40-41 0.2016774834465167 1102 42-43 0.27755178523598545 1102 44-45 0.27638993803252276 1102 46-47 0.3356059026136009 1102 48-49 0.3135038232372622 1102 50-51 0.37452639368731155 1102 52-53 0.30937622983741164 1102 54-55 0.4366934331900154 1102 56-57 0.39191535583066184 1102 58-59 0.32797636013537357 1102 60-61 0.37648093438572516 1102 62-63 0.28402792228189355 1102 64-65 0.3871620640483471 1102 66-67 0.3641288316832245 1102 68-69 0.37561735506579197 1102 70-71 0.30450638639427297 1102 72-73 0.2395079599387202 1102 74-75 0.2518530547230924 1102 76-77 0.15373159744016363 1102 78-79 0.07090358856552825 1102 80-81 0.20718492602807004 1102 82-83 0.1657871082022524 1102 84-85 0.2478909627824244 1102 86-87 0.23811780483309164 1102 88-89 0.25855370191994353 1102 90-91 0.2832919180253235 1102 92-93 0.32521122359519694 1102 94-95 0.33787026925702435 1102 96-97 0.42651311544980786 1102 98-99 0.3898902997005713 1102 100 0.29459584328343524 1103 1 0.06622467532233145 1103 2 0.13603847239713218 1103 3 0.1382374706362164 1103 4 0.20368407077587136 1103 5 0.14471141679135258 1103 6 0.22740801491495688 1103 7 0.30586966232117874 1103 8 0.17760887912709222 1103 9 0.39025568899791097 1103 10-11 0.34804086489243247 1103 12-13 0.3487139963962491 1103 14-15 0.385749446492035 1103 16-17 0.4567340428400115 1103 18-19 0.4013861929538649 1103 20-21 0.4841884876459659 1103 22-23 0.5067059561750966 1103 24-25 0.4091158995413551 1103 26-27 0.34653651634113203 1103 28-29 0.40678981067894426 1103 30-31 0.4260382279941055 1103 32-33 0.386484488950984 1103 34-35 0.29538982161437843 1103 36-37 0.30036283173947 1103 38-39 0.35567730943402864 1103 40-41 0.3624733072314399 1103 42-43 0.29741852087917664 1103 44-45 0.28222693396233467 1103 46-47 0.2892626560424745 1103 48-49 0.25509324759680396 1103 50-51 0.3616755967519225 1103 52-53 0.3139284667290241 1103 54-55 0.2805706264725032 1103 56-57 0.26603248481106334 1103 58-59 0.27586879951014254 1103 60-61 0.2145617480806976 1103 62-63 0.1268704676866932 1103 64-65 0.1940473891501071 1103 66-67 0.18509947675624971 1103 68-69 0.11508382734407263 1103 70-71 0.1581817378325141 1103 72-73 0.30415635067648594 1103 74-75 0.2538208096227095 1103 76-77 0.13034941048527315 1103 78-79 0.21106844816124948 1103 80-81 0.2095406738818113 1103 82-83 0.20390982086087206 1103 84-85 0.1882073854660078 1103 86-87 0.18811374319885843 1103 88-89 0.18483076523013153 1103 90-91 0.06340252273853153 1103 92-93 0.16851230612760304 1103 94-95 0.10627374772608889 1103 96-97 0.11493446510948502 1103 98-99 0.11767957356587644 1103 100 0.01967023532092327 1104 1 0.10003477429074437 1104 2 0.1309701414671416 1104 3 0.1500893384647064 1104 4 0.11002766247879237 1104 5 0.12563848048198167 1104 6 0.1422007933842977 1104 7 0.17849995635062044 1104 8 0.0914071976090014 1104 9 0.2160261964934591 1104 10-11 0.21458089389687274 1104 12-13 0.1962885462317061 1104 14-15 0.27767850372445224 1104 16-17 0.24091594094650048 1104 18-19 0.3783358018458216 1104 20-21 0.3125701908146823 1104 22-23 0.30392367640959606 1104 24-25 0.21336249788735984 1104 26-27 0.21759898886702445 1104 28-29 0.2838463888533127 1104 30-31 0.29577911891522746 1104 32-33 0.31169585984219594 1104 34-35 0.28284609413287853 1104 36-37 0.32437635721695557 1104 38-39 0.3416055593898619 1104 40-41 0.3124912295932063 1104 42-43 0.33589857621767294 1104 44-45 0.32975174796006 1104 46-47 0.30250915578981363 1104 48-49 0.3314006556432787 1104 50-51 0.22120921478890665 1104 52-53 0.30942656459642137 1104 54-55 0.2960225010796478 1104 56-57 0.2956843647589693 1104 58-59 0.22631552336403615 1104 60-61 0.29613404247166386 1104 62-63 0.3704412697457613 1104 64-65 0.20415657690250555 1104 66-67 0.28519394146819366 1104 68-69 0.2253738820252451 1104 70-71 0.17750784675939713 1104 72-73 0.24737122182035165 1104 74-75 0.31739666486343054 1104 76-77 0.2131032198858378 1104 78-79 0.18331805890192499 1104 80-81 0.13942816355976362 1104 82-83 0.1415922540705452 1104 84-85 0.13757504448716773 1104 86-87 0.08390984526491252 1104 88-89 0.10374093670964513 1104 90-91 0.11415477407474484 1104 92-93 0.20660400083326635 1104 94-95 0.2966116689786169 1104 96-97 0.26268122881367617 1104 98-99 0.27002799352224827 1104 100 0.30029641901845494 1105 1 0.08579665631712885 1105 2 0.13289153983987134 1105 3 0.10853078906981395 1105 4 0.1347681068889699 1105 5 0.10827422882154991 1105 6 0.1582940801790116 1105 7 0.20863934484427205 1105 8 0.19653820855862847 1105 9 0.2634307394453046 1105 10-11 0.2660347066926221 1105 12-13 0.25732859237492534 1105 14-15 0.26619892071362017 1105 16-17 0.24075086131782086 1105 18-19 0.25914991625292316 1105 20-21 0.2657248921737789 1105 22-23 0.2009902262354757 1105 24-25 0.15922317562326072 1105 26-27 0.19892312024668257 1105 28-29 0.22080549358793178 1105 30-31 0.2994032098652042 1105 32-33 0.17046010537391965 1105 34-35 0.20295929229861542 1105 36-37 0.3772963637806015 1105 38-39 0.27450649777364333 1105 40-41 0.34322742542707374 1105 42-43 0.3334479592449213 1105 44-45 0.2289551893266406 1105 46-47 0.18042164974760766 1105 48-49 0.20657883290716228 1105 50-51 0.24228862303279897 1105 52-53 0.27928507553862403 1105 54-55 0.20796079787305644 1105 56-57 0.1332209269312159 1105 58-59 0.20346422748895066 1105 60-61 0.2697622011023242 1105 62-63 0.304661856658214 1105 64-65 0.27044655063804157 1105 66-67 0.2806950273362929 1105 68-69 0.3398636475825185 1105 70-71 0.26465839938074964 1105 72-73 0.3234941834082008 1105 74-75 0.3560496398059243 1105 76-77 0.3259099393186702 1105 78-79 0.36460539334040476 1105 80-81 0.4828366674341993 1105 82-83 0.4115498375024842 1105 84-85 0.32453217222025543 1105 86-87 0.30063555014010035 1105 88-89 0.325491314564065 1105 90-91 0.43722499815798344 1105 92-93 0.4800827475724496 1105 94-95 0.41465781318308714 1105 96-97 0.40975074414185997 1105 98-99 0.34834802163520706 1105 100 0.41658068416203875 1106 1 -0.11606510003192128 1106 2 -0.18077992093235196 1106 3 -0.11772965103681088 1106 4 -0.08461593577240478 1106 5 -0.0928095178667192 1106 6 -0.15466561753594732 1106 7 -0.03953594915935099 1106 8 -0.02616186453942504 1106 9 0.01493770047129317 1106 10-11 0.09805450714591757 1106 12-13 0.013916787254053986 1106 14-15 0.07291502525099958 1106 16-17 0.062020986558522395 1106 18-19 0.022934640812671603 1106 20-21 0.0705822125070199 1106 22-23 0.04456283752618617 1106 24-25 0.06660511223947196 1106 26-27 0.06743250482409735 1106 28-29 0.0956314022884115 1106 30-31 0.1077004596881892 1106 32-33 0.08740536586511638 1106 34-35 0.14252024800673269 1106 36-37 0.11701113097213067 1106 38-39 0.14563912848714722 1106 40-41 0.20731660982753652 1106 42-43 0.09923285377793434 1106 44-45 0.11410166235746999 1106 46-47 0.10604700574850057 1106 48-49 0.1278317340309485 1106 50-51 0.10295906492033424 1106 52-53 0.005726768739741317 1106 54-55 0.09020829566125599 1106 56-57 0.11674933562334644 1106 58-59 0.15770865334456374 1106 60-61 0.23712882580086614 1106 62-63 0.1878616344515862 1106 64-65 0.07844008067245767 1106 66-67 0.03095571667338959 1106 68-69 0.12477912885275799 1106 70-71 -0.0037189455699717655 1106 72-73 0.04901421327766542 1106 74-75 0.11382884118938819 1106 76-77 0.10738292478929878 1106 78-79 0.07012611074218711 1106 80-81 0.0672026719213008 1106 82-83 0.03598614455633253 1106 84-85 0.05459707718084772 1106 86-87 0.03805317159340049 1106 88-89 0.045175078345149444 1106 90-91 0.06516330359135125 1106 92-93 0.11572087782831275 1106 94-95 0.012366241708615888 1106 96-97 0.057531459051332945 1106 98-99 0.02601944277883561 1106 100 -0.04650312745983598 1107 1 -0.024610879469971536 1107 2 -0.04445710159794203 1107 3 0.016214485854025185 1107 4 -0.05762975795256864 1107 5 -0.01602179151579719 1107 6 0.0779791217674557 1107 7 0.1166235565420024 1107 8 0.057868419041149366 1107 9 0.054505258691875724 1107 10-11 0.07440867214655356 1107 12-13 0.1043910356175175 1107 14-15 0.10808264679803159 1107 16-17 0.07630252687476968 1107 18-19 0.10081389080681902 1107 20-21 0.04425972232180442 1107 22-23 0.10172040704215135 1107 24-25 0.04314025135020216 1107 26-27 0.11314501200685356 1107 28-29 0.1993229032620647 1107 30-31 0.16607483815830903 1107 32-33 0.06569426753364382 1107 34-35 0.15554731264723642 1107 36-37 0.22967299066974078 1107 38-39 0.1832688787533101 1107 40-41 0.19193624431894563 1107 42-43 0.24206430875879192 1107 44-45 0.23093635737176044 1107 46-47 0.2512997355427373 1107 48-49 0.1379385267200277 1107 50-51 0.1876682766705855 1107 52-53 0.19667763441371733 1107 54-55 0.20534549640494504 1107 56-57 0.2602449418292423 1107 58-59 0.2958543681581034 1107 60-61 0.2506758650458778 1107 62-63 0.29627126897312195 1107 64-65 0.27237113735960605 1107 66-67 0.3212748708619344 1107 68-69 0.3247255609797577 1107 70-71 0.23684797451839756 1107 72-73 0.19808884044086028 1107 74-75 0.20036894535352445 1107 76-77 0.15112436140501728 1107 78-79 0.09842876945641876 1107 80-81 0.16689512603685586 1107 82-83 0.2553280988171025 1107 84-85 0.17281674857404639 1107 86-87 0.02059638459488511 1107 88-89 0.009741703590009365 1107 90-91 0.11788587740827694 1107 92-93 0.1373704924803647 1107 94-95 0.1428201222269898 1107 96-97 0.08770053279535617 1107 98-99 -0.04029652932507943 1107 100 -0.0712911913045815 1108 1 -0.12646497119836297 1108 2 -0.07626129021299732 1108 3 -0.06346309967846864 1108 4 0.025019244041530442 1108 5 0.043598169278638466 1108 6 0.048439454597662746 1108 7 0.05908178367950967 1108 8 0.038930373525275286 1108 9 0.04851970320270027 1108 10-11 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2214 92-93 0.09364655716238701 2214 94-95 0.017288926772554447 2214 96-97 0.04854265047736561 2214 98-99 -0.04168694415841401 2214 100 0.037916653038902126 2215 1 -0.21284854002238163 2215 2 -0.25298681751333874 2215 3 -0.3378310718446862 2215 4 -0.2816507106212711 2215 5 -0.3390456478785069 2215 6 -0.2909306533903049 2215 7 -0.2986057301207836 2215 8 -0.4025590474503531 2215 9 -0.48301699475495496 2215 10-11 -0.4074233133908933 2215 12-13 -0.4529668323582925 2215 14-15 -0.4518334807641864 2215 16-17 -0.6104455194541245 2215 18-19 -0.5107924136896074 2215 20-21 -0.578483216877558 2215 22-23 -0.5612894817093945 2215 24-25 -0.561834776380536 2215 26-27 -0.485815116660433 2215 28-29 -0.41856882625954484 2215 30-31 -0.5064535373338117 2215 32-33 -0.6020012602434122 2215 34-35 -0.562798541597175 2215 36-37 -0.4510905164317478 2215 38-39 -0.5327975882131 2215 40-41 -0.5218040958280739 2215 42-43 -0.5060869754518365 2215 44-45 -0.40758806278666526 2215 46-47 -0.45541673655667836 2215 48-49 -0.39337780065373096 2215 50-51 -0.3564579192565631 2215 52-53 -0.3497249232407569 2215 54-55 -0.3830816470829461 2215 56-57 -0.6119092669412609 2215 58-59 -0.5844074817762532 2215 60-61 -0.690173054394343 2215 62-63 -0.7986276317295591 2215 64-65 -0.9602674182550288 2215 66-67 -0.8978088004288907 2215 68-69 -0.7771629877491364 2215 70-71 -0.6780925285492998 2215 72-73 -0.8019414150658477 2215 74-75 -0.7435904488352492 2215 76-77 -0.6792800649964832 2215 78-79 -0.721261660372086 2215 80-81 -0.7526564205829125 2215 82-83 -0.7980736008329323 2215 84-85 -0.6939093093045265 2215 86-87 -0.7120752434184574 2215 88-89 -0.7065972715954629 2215 90-91 -0.7053990109468167 2215 92-93 -0.7225573616989394 2215 94-95 -0.8308540397372077 2215 96-97 -0.7942032521893978 2215 98-99 -0.6721605648337707 2215 100 -0.6954422635180073 2216 1 -0.38658071966803575 2216 2 -0.4001705834795537 2216 3 -0.3850997162616636 2216 4 -0.4401521527308674 2216 5 -0.41663444062675836 2216 6 -0.5478482471274333 2216 7 -0.4751524956749691 2216 8 -0.32890268156654656 2216 9 -0.5079716713103721 2216 10-11 -0.4386046071692249 2216 12-13 -0.540493614393732 2216 14-15 -0.632170110348035 2216 16-17 -0.731225494732243 2216 18-19 -0.645776138452689 2216 20-21 -0.49965688807657216 2216 22-23 -0.5097795929578481 2216 24-25 -0.5571755262775255 2216 26-27 -0.5911128092850717 2216 28-29 -0.520625892596577 2216 30-31 -0.7261313288459732 2216 32-33 -0.8403315033418863 2216 34-35 -0.9945750146091257 2216 36-37 -1.1197590372393336 2216 38-39 -0.9543713418183799 2216 40-41 -0.8847781378552639 2216 42-43 -0.9614827315293013 2216 44-45 -0.8901391546696473 2216 46-47 -0.7796145115875817 2216 48-49 -0.7292535732124463 2216 50-51 -0.7156664071016436 2216 52-53 -0.814093691267125 2216 54-55 -1.0027429573425195 2216 56-57 -1.0123938157669699 2216 58-59 -0.9650714702393728 2216 60-61 -0.922044706639916 2216 62-63 -0.9710736556273716 2216 64-65 -0.9656934586340995 2216 66-67 -0.9809116022418252 2216 68-69 -0.9570202188987054 2216 70-71 -1.0321804972349184 2216 72-73 -1.0276991406529916 2216 74-75 -1.0082707125352925 2216 76-77 -0.8189646550253187 2216 78-79 -0.9089662339196778 2216 80-81 -0.8990956459640387 2216 82-83 -0.9743430693125354 2216 84-85 -0.8137362561524846 2216 86-87 -0.8504536941807181 2216 88-89 -0.9055049765810423 2216 90-91 -1.0053619281285258 2216 92-93 -1.1053711235448418 2216 94-95 -1.039554286955994 2216 96-97 -1.0550135117691255 2216 98-99 -1.0132236056248765 2216 100 -1.0696033677619283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36315.0 3 6496.0 4 3596.0 5 2959.0 6 2362.0 7 2505.0 8 2870.0 9 3267.0 10 3675.0 11 4098.0 12 4676.0 13 4862.0 14 5339.0 15 5336.0 16 5393.0 17 5541.0 18 5365.0 19 5455.0 20 5448.0 21 5634.0 22 6361.0 23 8793.0 24 12760.0 25 16238.0 26 16164.0 27 15191.0 28 16883.0 29 20705.0 30 25034.0 31 30155.0 32 39019.0 33 51208.0 34 72396.0 35 108426.0 36 187939.0 37 372070.0 38 557071.0 39 210869.0 40 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91420010192956 19.924580600112947 13.435100430286854 23.726118867670635 2 15.180295203303837 20.058718624651547 40.95944516567116 23.801541006373455 3 18.189186354375583 25.254777441217634 29.862225767934543 26.693810436472244 4 11.915822981991738 16.69679700067184 39.751906900446976 31.635473116889447 5 12.531417303932974 38.21324500012453 36.14573071760488 13.109606978337625 6 30.670130954752995 38.582581194914425 17.902943408384665 12.844344441947914 7 26.99262277142356 32.547880974423734 22.738726555002245 17.72076969915046 8 24.46616359641173 37.53528434705275 20.334065784968736 17.66448627156678 9 24.196847009418057 17.54776467520657 21.400259790228134 36.855128525147244 10-11 23.41442163141363 27.469357696834052 29.134044391821867 19.982176279930457 12-13 24.033210226489736 24.667383612090475 29.146720107192 22.15268605422779 14-15 21.33296761395935 26.838528271035155 26.083768287625038 25.744735827380456 16-17 21.023438793554497 30.25743597215691 26.63894720807476 22.080178026213833 18-19 20.931105160720175 29.28505655447633 28.56980837591867 21.214029908884825 20-21 22.23662183209319 28.47892778579457 28.096338601099834 21.188111781012406 22-23 21.759140187104027 28.199737612549676 28.240237351248222 21.80088484909808 24-25 21.699167052131326 28.333307297368933 28.143306537471798 21.824219113027947 26-27 21.45903623309294 28.20785525540706 28.266947238656552 22.06616127284344 28-29 21.485981966937796 28.30255153154938 28.323345869979494 21.88812063153333 30-31 21.74118650668546 28.827852413891762 27.902750217903304 21.52821086151947 32-33 21.31687484817201 28.403072879289166 28.527157904552546 21.752894367986276 34-35 21.405840551302543 28.13338589986605 28.61056221919026 21.85021132964114 36-37 21.79083470597412 28.578553325339833 27.936716293623558 21.69389567506249 38-39 21.687587741480314 28.71898697154096 28.051549005545777 21.54187628143294 40-41 21.39718496439808 28.402238781255175 28.423063421096206 21.777512833250537 42-43 21.34613861270724 28.294312307909554 28.355140122540494 22.00440895684272 44-45 21.54330145442022 28.459586192280177 28.30789201790871 21.689220335390893 46-47 21.51811773134126 28.67123566403774 28.172696019313147 21.63795058530785 48-49 21.48682405858788 28.456758904129188 28.2260305746166 21.830386462666336 50-51 21.48854913297248 28.364102814077597 28.264811102021444 21.88253695092849 52-53 21.596099601837444 28.22092683468319 28.330395706658397 21.852577856820968 54-55 21.694651966988772 28.768929349677535 27.992841244856237 21.543577438477463 56-57 21.43183551545268 28.47318050241506 28.145723522039432 21.94926046009282 58-59 21.338173622595715 28.06512138736592 28.53105750730931 22.06564748272905 60-61 21.59703744124351 28.55001768158056 28.085925859212235 21.76701901796369 62-63 21.4270822598988 28.5045843877827 28.15342062800987 21.914912724308632 64-65 21.417489076115533 28.301463449523638 28.332112577930772 21.948934896430053 66-67 21.3064175174438 28.334375281458446 28.171884354354194 22.18732284674356 68-69 21.812279302881876 28.42123248661579 28.059799150721727 21.706689059780608 70-71 21.722720449620464 28.246036776678135 28.182544679550325 21.84869809415107 72-73 21.449945077837963 28.33257799357568 28.370190470110927 21.84728645847543 74-75 21.49774571041312 28.42407397449756 28.221067389762844 21.857112925326476 76-77 21.496636838111332 28.410460698218632 28.080911887674766 22.011990575995274 78-79 21.674636256681715 28.48017349286286 28.17283177623065 21.672358474224772 80-81 21.57665480080205 28.230684908723063 28.302854719918898 21.88980557055599 82-83 21.381291869539066 28.317786441082887 28.46592313154554 21.834998557832506 84-85 21.421751947259853 28.227408912254333 28.269755904942524 22.081083235543293 86-87 21.586598404460982 28.375531113646403 28.17987578481939 21.85799469707322 88-89 21.315115815299347 28.240489882481807 28.318907732831626 22.125486569387217 90-91 21.311242711360613 28.197798670685646 28.337811519982882 22.15314709797086 92-93 21.516741631832407 28.32359103977356 28.209289465822874 21.95037786257116 94-95 21.27139552079118 28.067777111586157 28.431519503054005 22.229307864568657 96-97 21.23985115154687 28.10458693388977 28.488969975801933 22.16659193876143 98-99 21.317746413372596 28.17621542513959 28.297722848136114 22.2083153133517 100 21.45354399280427 28.086393267033444 28.223749937757773 22.23631280240451 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 237.0 1 227.0 2 200.0 3 183.0 4 189.0 5 201.5 6 214.5 7 201.5 8 167.5 9 147.0 10 139.5 11 145.5 12 153.0 13 150.0 14 168.0 15 226.0 16 283.5 17 382.0 18 571.0 19 828.0 20 1185.0 21 1734.0 22 2708.0 23 4158.0 24 6071.5 25 8527.0 26 11620.0 27 15540.0 28 20507.0 29 26213.0 30 32028.5 31 37998.0 32 44271.5 33 50545.0 34 56059.0 35 60475.0 36 64146.5 37 67789.0 38 70441.0 39 71005.5 40 71418.5 41 71730.5 42 70857.5 43 69339.0 44 67658.5 45 66648.5 46 65567.0 47 62934.0 48 60000.5 49 58312.5 50 56548.0 51 54288.0 52 50857.5 53 47663.0 54 45790.5 55 43316.0 56 40012.5 57 36701.5 58 33071.5 59 29113.5 60 25500.0 61 21608.5 62 17921.5 63 14682.0 64 11697.5 65 9319.0 66 7331.0 67 5474.5 68 4113.5 69 3208.0 70 2402.0 71 1698.0 72 1286.5 73 946.0 74 649.5 75 428.0 76 260.0 77 177.5 78 115.5 79 67.0 80 47.5 81 31.0 82 24.5 83 19.5 84 15.0 85 11.5 86 9.5 87 7.5 88 4.5 89 3.0 90 2.5 91 1.5 92 1.0 93 2.5 94 1.5 95 0.0 96 0.5 97 2.5 98 2.0 99 0.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0661786038749955 2 0.04452496468709697 3 0.05834305717619603 4 0.0779319239231947 5 0.09164413064609377 6 0.09339124578839364 7 0.0955619039954935 8 0.10662696656339274 9 0.10170327843509308 10-11 0.10675932377114275 12-13 0.11223891217199237 14-15 0.11305952686004231 16-17 0.07758779518304473 18-19 0.10220623582454305 20-21 0.09619721859269346 22-23 0.06149315872064581 24-25 0.021944825044948508 26-27 0.19226207997763695 28-29 0.06856103361449534 30-31 0.08063201096129452 32-33 0.07478182237874491 34-35 0.02263308252524846 36-37 0.05524589851484624 38-39 0.08134673988314446 40-41 0.08685279972554409 42-43 0.08118791123384449 44-45 0.07824958122179468 46-47 0.08555569908959418 48-49 0.08171734006484445 50-51 0.07729660932599475 52-53 0.08118791123384449 54-55 0.07141994930189514 56-57 0.07435827931394494 58-59 0.07081110614624518 60-61 0.06726393297854544 62-63 0.073564136067445 64-65 0.07070522038004519 66-67 0.07083757758779519 68-69 0.07110229200329517 70-71 0.06308144521364571 72-73 0.06122844430514584 74-75 0.06376970269394566 76-77 0.05799892843604606 78-79 0.05437234094369631 80-81 0.04868098101044669 82-83 0.05529884139794624 84-85 0.04523969360894692 86-87 0.043095506843397066 88-89 0.045795593881496885 90-91 0.04166604899969717 92-93 0.04447202180399698 94-95 0.04320139260959706 96-97 0.045927951089246875 98-99 0.04645737992024684 100 0.05516648419019625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1888828.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.79452004884868 #Duplication Level Percentage of deduplicated Percentage of total 1 81.32477486237501 59.19997954187263 2 12.255301540450805 17.842375873820647 3 3.2919468289055724 7.1890706830953155 4 1.2893436282605746 3.7542860238907876 5 0.6201908815967766 2.257324878225485 6 0.3602739601950774 1.5735582011099203 7 0.20202744592557903 1.0294543673993077 8 0.13743310182758806 0.8003501349091056 9 0.09061478647007234 0.593663390137604 >10 0.41406618678545803 4.97698558045503 >50 0.012204610499961348 0.5916190755307915 >100 0.0018221667075522185 0.19133224955335473 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.29428830999964E-5 0.0 7 0.0 0.0 0.0 5.29428830999964E-5 0.0 8 0.0 0.0 0.0 5.29428830999964E-5 0.0 9 0.0 0.0 0.0 1.588286492999892E-4 0.0 10-11 0.0 0.0 0.0 1.588286492999892E-4 0.0 12-13 0.0 0.0 0.0 2.3824297394998381E-4 0.0 14-15 0.0 0.0 0.0 5.029573894499658E-4 0.0 16-17 0.0 0.0 0.0 7.147289218499514E-4 0.0 18-19 0.0 0.0 0.0 7.412003633999496E-4 0.0 20-21 0.0 0.0 0.0 7.94143246499946E-4 0.0 22-23 0.0 0.0 0.0 9.26500454249937E-4 0.0 24-25 0.0 0.0 0.0 0.0010059147788999316 0.0 26-27 0.0 0.0 0.0 0.0011118005450999244 0.0 28-29 0.0 0.0 0.0 0.0015353436098998956 0.0 30-31 0.0 0.0 0.0 0.003203044427549782 0.0 32-33 0.0 0.0 0.0 0.007544360841749488 0.0 34-35 0.0 0.0 0.0 0.014003392579949049 0.0 36-37 0.0 0.0 0.0 0.025068455147848293 0.0 38-39 0.0 0.0 0.0 0.04433966459624698 0.0 40-41 0.0 0.0 0.0 0.07104934912019517 0.0 42-43 0.0 0.0 0.0 0.09781197652724336 0.0 44-45 0.0 0.0 0.0 0.1280953056604413 0.0 46-47 0.0 0.0 0.0 0.16727303915443864 0.0 48-49 0.0 0.0 0.0 0.21198330393238557 0.0 50-51 0.0 0.0 0.0 0.26389380081193203 0.0 52-53 0.0 0.0 0.0 0.3159101834576785 0.0 54-55 0.0 7.94143246499946E-5 0.0 0.36972662412882484 0.0 56-57 0.0 1.588286492999892E-4 0.0 0.4350581418742204 0.0 58-59 0.0 1.588286492999892E-4 0.0 0.5131224230051652 0.0 60-61 0.0 1.588286492999892E-4 0.0 0.5962427494721594 0.0 62-63 0.0 1.588286492999892E-4 0.0 0.6819043343279536 0.0 64-65 0.0 1.588286492999892E-4 0.0 0.7720925356887975 0.0 66-67 0.0 1.588286492999892E-4 0.0 0.8763370725126904 0.0 68-69 0.0 1.588286492999892E-4 0.0 0.9933408441636825 0.0 70-71 0.0 1.588286492999892E-4 0.0 1.1260157092122736 0.0 72-73 0.0 1.588286492999892E-4 0.0 1.2694379795301636 0.0 74-75 0.0 1.588286492999892E-4 0.0 1.4175456950024037 0.0 76-77 0.0 1.588286492999892E-4 0.0 1.572985999783993 0.0 78-79 0.0 1.588286492999892E-4 0.0 1.7527270879084806 0.0 80-81 0.0 1.588286492999892E-4 0.0 1.9606073184006167 0.0 82-83 0.0 1.588286492999892E-4 0.0 2.1803996975902518 0.0 84-85 0.0 1.588286492999892E-4 0.0 2.392912430353637 0.0 86-87 0.0 1.588286492999892E-4 0.0 2.6123342093615722 0.0 88 0.0 1.588286492999892E-4 0.0 2.79241942622621 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 1955 0.0 31.741886 4 ATCAACG 2030 0.0 30.569153 3 CAACGCA 2035 0.0 30.49566 5 GTATCAA 2220 0.0 30.283083 1 AACGCAG 2180 0.0 29.105776 6 GGTATCA 1485 0.0 26.276571 1 GTACATG 4575 0.0 25.99839 1 TACATGG 4595 0.0 25.06606 2 TATCAAC 2665 0.0 23.814533 2 ACGCAGA 2670 0.0 22.88411 7 ACATGGG 4890 0.0 22.59251 3 CGCAGAG 2710 0.0 22.218904 8 GTGTAGG 695 0.0 21.646229 1 GTGTACG 205 7.5454045E-7 20.639809 1 ACGCGTT 80 5.3545293E-5 20.593533 10-11 CTAGTAC 440 0.0 20.300486 3 AGTACCG 235 1.2625605E-7 20.00602 5 GAGTACA 3245 0.0 19.993158 1 CATGGGG 3405 0.0 19.605448 4 GTCTACG 255 2.9833063E-7 18.43643 1 >>END_MODULE