FastQCFastQC Report
Wed 25 May 2016
SRR1033047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888828
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.14882244439408987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.13029243530909113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.048.904981
GTATCAA47100.043.4415931
TCAACGC53950.037.4579244
ATCAACG53950.037.3718033
CAACGCA54600.037.186115
AACGCAG57100.035.5586
TATCAAC59750.033.988672
ACGCAGA65850.030.547647
CGCAGAG66200.030.3151388
GTGGTAT14050.026.5086541
GCAGAGT76100.026.0625829
GTACATG47550.025.4811781
TGGTATC14100.024.0606962
TACATGG47750.023.9788152
CACCGTT1652.6657835E-622.7874497
TACGACC1252.4254131E-422.558384
ACATGGG49900.022.4158153
GAGTACT52600.021.62230912-13
AGAGTAC69450.021.18174610-11
TACGGGT1354.080368E-420.887394
Produced by FastQC (version 0.11.5)