##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1888828 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.675192765037366 34.0 31.0 34.0 31.0 34.0 2 32.66673937489279 34.0 31.0 34.0 31.0 34.0 3 32.77748847433435 34.0 31.0 34.0 31.0 34.0 4 36.282097152308204 37.0 37.0 37.0 35.0 37.0 5 36.26789204734364 37.0 37.0 37.0 35.0 37.0 6 36.337814242482644 37.0 37.0 37.0 35.0 37.0 7 36.28068145961411 37.0 37.0 37.0 35.0 37.0 8 36.285969394778135 37.0 37.0 37.0 35.0 37.0 9 38.07681747623394 39.0 39.0 39.0 35.0 39.0 10-11 38.04179152363264 39.0 38.5 39.0 35.0 39.0 12-13 38.024113630251136 39.0 38.0 39.0 35.0 39.0 14-15 39.51251755056575 41.0 39.0 41.0 37.0 41.0 16-17 39.5082299711779 41.0 39.0 41.0 36.5 41.0 18-19 39.52338434203644 41.0 39.5 41.0 36.5 41.0 20-21 39.51711881653597 41.0 39.5 41.0 37.0 41.0 22-23 39.45158399811947 41.0 39.0 41.0 36.0 41.0 24-25 39.404672368262226 41.0 39.0 41.0 36.0 41.0 26-27 39.30160528115847 41.0 39.0 41.0 36.0 41.0 28-29 39.24625826173691 41.0 39.0 41.0 36.0 41.0 30-31 39.24409739796318 41.0 39.0 41.0 36.0 41.0 32-33 39.207457481570586 41.0 39.0 41.0 35.5 41.0 34-35 39.13539268795253 41.0 39.0 41.0 35.0 41.0 36-37 39.05649323284068 41.0 39.0 41.0 35.0 41.0 38-39 38.96372883078819 41.0 39.0 41.0 35.0 41.0 40-41 38.86235379822832 41.0 39.0 41.0 35.0 41.0 42-43 38.752428225333375 41.0 39.0 41.0 35.0 41.0 44-45 38.59672426499395 41.0 38.5 41.0 35.0 41.0 46-47 38.41455918696673 41.0 38.0 41.0 34.5 41.0 48-49 38.21950463461999 40.0 38.0 41.0 34.0 41.0 50-51 38.08697033292603 40.0 38.0 41.0 34.0 41.0 52-53 37.959805763150484 40.0 38.0 41.0 34.0 41.0 54-55 37.82103902525799 40.0 38.0 41.0 33.0 41.0 56-57 37.684372531538074 40.0 37.0 41.0 33.0 41.0 58-59 37.516664831313385 40.0 37.0 41.0 33.0 41.0 60-61 37.334363954791016 40.0 36.5 41.0 33.0 41.0 62-63 37.11095081182617 39.5 36.0 41.0 32.5 41.0 64-65 36.85344642286116 39.0 35.5 41.0 32.0 41.0 66-67 36.58824281512133 39.0 35.0 41.0 32.0 41.0 68-69 36.28363381949018 38.5 35.0 40.5 31.5 41.0 70-71 35.96066979100267 37.0 35.0 40.0 31.0 41.0 72-73 35.612571922906696 37.0 35.0 39.5 31.0 41.0 74-75 35.26647979593695 36.5 35.0 39.0 31.0 41.0 76-77 34.907210185363624 36.0 35.0 39.0 31.0 40.5 78-79 34.54218568339732 35.5 35.0 37.5 30.0 39.5 80-81 34.20097568439265 35.0 34.0 37.0 30.0 39.0 82-83 33.886258833520046 35.0 34.0 37.0 30.0 39.0 84-85 33.65083030323566 35.0 34.0 36.0 30.0 38.0 86-87 33.42386866353104 35.0 34.0 36.0 30.0 37.0 88-89 33.20586125364512 35.0 34.0 36.0 30.0 37.0 90-91 33.00855901119636 35.0 34.0 35.0 30.0 36.5 92-93 32.827183311556155 35.0 34.0 35.0 29.0 36.0 94-95 32.66594893764811 35.0 34.0 35.0 29.0 36.0 96-97 32.51125380394615 35.0 34.0 35.0 29.0 36.0 98-99 32.38194398854739 35.0 34.0 35.0 29.0 36.0 100 32.274415669399225 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0967970689801092 1101 2 0.16486719799998184 1101 3 0.1548554409228089 1101 4 0.10533950413245208 1101 5 0.12359023829653637 1101 6 0.11108346781957579 1101 7 0.16016876223756782 1101 8 0.15925419920495898 1101 9 0.20622528477716884 1101 10-11 0.22523332949594987 1101 12-13 0.22828603149872606 1101 14-15 0.2995068971153785 1101 16-17 0.29566015953000857 1101 18-19 0.22790971032595309 1101 20-21 0.2834173243373286 1101 22-23 0.28346498609502646 1101 24-25 0.32239732510690544 1101 26-27 0.3372264603030928 1101 28-29 0.3106264770452114 1101 30-31 0.34268080521329836 1101 32-33 0.3514730189895019 1101 34-35 0.3469498146797392 1101 36-37 0.31404693260639505 1101 38-39 0.3200346998385868 1101 40-41 0.3212523122026596 1101 42-43 0.3284657909921549 1101 44-45 0.31111598642145566 1101 46-47 0.22677758799332537 1101 48-49 0.17528127143480532 1101 50-51 0.25989698943064354 1101 52-53 0.26136689257812407 1101 54-55 0.22873923516272754 1101 56-57 0.21879234953967597 1101 58-59 0.16849709519849654 1101 60-61 0.24180348458327927 1101 62-63 0.2745755702251742 1101 64-65 0.20965434889097168 1101 66-67 0.2144630075869216 1101 68-69 0.26744687969270586 1101 70-71 0.2839205983672173 1101 72-73 0.2939410370548785 1101 74-75 0.26593754484059673 1101 76-77 0.2706396967028013 1101 78-79 0.2639440138395841 1101 80-81 0.2951832354459967 1101 82-83 0.29877581575039613 1101 84-85 0.35251022814161814 1101 86-87 0.2883172736488362 1101 88-89 0.29406497213327043 1101 90-91 0.25317889861710796 1101 92-93 0.2701336144136661 1101 94-95 0.2719180533708112 1101 96-97 0.3295172451851798 1101 98-99 0.21357737552148137 1101 100 0.20249541174467112 1102 1 0.18478544527511787 1102 2 0.11381451332983517 1102 3 0.20229691779622527 1102 4 0.11989643213109957 1102 5 0.13181168728015535 1102 6 0.1655788686633599 1102 7 0.1758861485777743 1102 8 0.19193007342152413 1102 9 0.2021115119171739 1102 10-11 0.20805813035437382 1102 12-13 0.20169185964613234 1102 14-15 0.28853020826397824 1102 16-17 0.30449322202044726 1102 18-19 0.31912339871843187 1102 20-21 0.30766591543697785 1102 22-23 0.3031319491000346 1102 24-25 0.31420088659858436 1102 26-27 0.3848816000735482 1102 28-29 0.29744692649661886 1102 30-31 0.3575823373785667 1102 32-33 0.26549483366644466 1102 34-35 0.26265696127411076 1102 36-37 0.2760260219091748 1102 38-39 0.2922480617785439 1102 40-41 0.3332172265933906 1102 42-43 0.3426767984833816 1102 44-45 0.3338157293608788 1102 46-47 0.3742144784582422 1102 48-49 0.3525364680711789 1102 50-51 0.3530302491227957 1102 52-53 0.28868796167964206 1102 54-55 0.2696538093555745 1102 56-57 0.27049101113977514 1102 58-59 0.18107008976021888 1102 60-61 0.2557046670175751 1102 62-63 0.22834581970362677 1102 64-65 0.29631779352484244 1102 66-67 0.3178251359908373 1102 68-69 0.3922990338359327 1102 70-71 0.2692850363257193 1102 72-73 0.2859739882521737 1102 74-75 0.3479448149215827 1102 76-77 0.2566260710512793 1102 78-79 0.20545788336055182 1102 80-81 0.21411694119754543 1102 82-83 0.2234789739706784 1102 84-85 0.26378012844335075 1102 86-87 0.2823997674800509 1102 88-89 0.28863707546297945 1102 90-91 0.23260497679721936 1102 92-93 0.33414699065324527 1102 94-95 0.3435908010701212 1102 96-97 0.33171297092958696 1102 98-99 0.3818515945295502 1102 100 0.45897942942742986 1103 1 0.07743431485818064 1103 2 0.16806084503281227 1103 3 0.1477940618260547 1103 4 0.0989897043546577 1103 5 0.04449082672674365 1103 6 0.101599578722535 1103 7 0.06701724957993349 1103 8 0.12231366270907529 1103 9 0.12796103478203946 1103 10-11 0.14098237873827202 1103 12-13 0.15035814722693885 1103 14-15 0.2109187912239321 1103 16-17 0.25464628149987334 1103 18-19 0.1408230002507338 1103 20-21 0.216398856573214 1103 22-23 0.18789590402016643 1103 24-25 0.1758691066622049 1103 26-27 0.22521768961761524 1103 28-29 0.23239451003802003 1103 30-31 0.19724398187013747 1103 32-33 0.1558339159936324 1103 34-35 0.23990646532718785 1103 36-37 0.24682928489784928 1103 38-39 0.28405852454831404 1103 40-41 0.37536523254475185 1103 42-43 0.32354970781059933 1103 44-45 0.35942967744036736 1103 46-47 0.3176091923481792 1103 48-49 0.2507251929745564 1103 50-51 0.2940980741390433 1103 52-53 0.23897569639934346 1103 54-55 0.33416004289527024 1103 56-57 0.36163942752995837 1103 58-59 0.36762736307827026 1103 60-61 0.3963430276564921 1103 62-63 0.371535527950023 1103 64-65 0.4226132410741812 1103 66-67 0.3659661901345572 1103 68-69 0.2989541284078783 1103 70-71 0.34816624848838984 1103 72-73 0.35133423383140894 1103 74-75 0.3228225383542238 1103 76-77 0.291563607392213 1103 78-79 0.34016198261834774 1103 80-81 0.3307414238736044 1103 82-83 0.2383798273414115 1103 84-85 0.2603117065806444 1103 86-87 0.31190219590977364 1103 88-89 0.2237973603759258 1103 90-91 0.1790298835934081 1103 92-93 0.26505645468854055 1103 94-95 0.24865223863211838 1103 96-97 0.2996998112346816 1103 98-99 0.26513946606220173 1103 100 0.31506504012619985 1104 1 0.22146437101833527 1104 2 0.24044099629960414 1104 3 0.2594940260840346 1104 4 0.185474343504076 1104 5 0.16728468156485832 1104 6 0.2046113058014285 1104 7 0.16471209492381433 1104 8 0.19094834848744568 1104 9 0.19203168072655075 1104 10-11 0.23354328909154276 1104 12-13 0.25816680213571885 1104 14-15 0.3429504937763994 1104 16-17 0.34772907151347 1104 18-19 0.2896729649195464 1104 20-21 0.3348681071411832 1104 22-23 0.28347537146412094 1104 24-25 0.2718220422320812 1104 26-27 0.3207743047664806 1104 28-29 0.33271056498212204 1104 30-31 0.2844778681776603 1104 32-33 0.23711798902238002 1104 34-35 0.19513702187557413 1104 36-37 0.26546597346163736 1104 38-39 0.21695738183085211 1104 40-41 0.27181788875174107 1104 42-43 0.19314090336051493 1104 44-45 0.18046937470137436 1104 46-47 0.17610803792212693 1104 48-49 0.15295126328362585 1104 50-51 0.13392672300864206 1104 52-53 0.09357873919527293 1104 54-55 0.17479250198709195 1104 56-57 0.15574037715837363 1104 58-59 0.08906892693528334 1104 60-61 0.10372221347800803 1104 62-63 0.09066592879468516 1104 64-65 0.07069611325333369 1104 66-67 0.1433514562071707 1104 68-69 0.14149026071086013 1104 70-71 0.2137021887293784 1104 72-73 0.13606997913461782 1104 74-75 0.08054725601283508 1104 76-77 0.13311850322683227 1104 78-79 0.09152811336017663 1104 80-81 0.10263096974458108 1104 82-83 0.09946740144447119 1104 84-85 0.12658071763601697 1104 86-87 0.2006927856941374 1104 88-89 0.10883632225521467 1104 90-91 0.18462754043718377 1104 92-93 0.1963277920562021 1104 94-95 0.2539614173463107 1104 96-97 0.3023155003366611 1104 98-99 0.25408357705143203 1104 100 0.2659765129026965 1105 1 0.06544954152977311 1105 2 0.08775000065197247 1105 3 0.0889635570305316 1105 4 0.036612305591120275 1105 5 0.09909962828960772 1105 6 0.13002447441099463 1105 7 0.09491405551624155 1105 8 0.09022580384922207 1105 9 0.1641967346542117 1105 10-11 0.1324512922903267 1105 12-13 0.10930130447005837 1105 14-15 0.19148523908422987 1105 16-17 0.18290546453346934 1105 18-19 0.13824008362762186 1105 20-21 0.1468746599127897 1105 22-23 0.1811666537769696 1105 24-25 0.1449705802888701 1105 26-27 0.14758723989216804 1105 28-29 0.1323782804481084 1105 30-31 0.1335491222787013 1105 32-33 0.1243530337470986 1105 34-35 0.1165696214447891 1105 36-37 0.05129739651722787 1105 38-39 0.08067488116114419 1105 40-41 0.08993498259901855 1105 42-43 0.11092951840173981 1105 44-45 0.08356960966801097 1105 46-47 0.20573204682654023 1105 48-49 0.275422307624801 1105 50-51 0.2866980343438854 1105 52-53 0.30533537425957746 1105 54-55 0.21604832302100618 1105 56-57 0.2106281180683638 1105 58-59 0.32499378802621237 1105 60-61 0.2639809238996733 1105 62-63 0.2828053953799028 1105 64-65 0.3397850516331644 1105 66-67 0.2516294829223966 1105 68-69 0.3529152537666391 1105 70-71 0.35548890881462825 1105 72-73 0.36695533973475136 1105 74-75 0.2946556579728252 1105 76-77 0.2814849315659629 1105 78-79 0.2834420782071092 1105 80-81 0.2852033551847626 1105 82-83 0.24451642119768024 1105 84-85 0.28887302126786807 1105 86-87 0.2593316399031167 1105 88-89 0.2714477880733597 1105 90-91 0.25423393425786145 1105 92-93 0.188383952738981 1105 94-95 0.16244799301159674 1105 96-97 0.233098699382154 1105 98-99 0.1977298894987385 1105 100 0.21664488769869195 1106 1 -0.008517835061752521 1106 2 0.05759108519600886 1106 3 0.05183693124339328 1106 4 0.03390908607780574 1106 5 -0.007542960422348699 1106 6 0.06059803392335539 1106 7 0.10556579318255643 1106 8 0.03770800320781831 1106 9 0.14457878776894972 1106 10-11 0.1041133825700058 1106 12-13 0.12937772559697436 1106 14-15 0.061621007618839485 1106 16-17 0.152850514467886 1106 18-19 0.1517839928313407 1106 20-21 0.11461381731329112 1106 22-23 0.16949107783736395 1106 24-25 0.09019377046747223 1106 26-27 0.12221216475300167 1106 28-29 0.21196480137702167 1106 30-31 0.19803535659258387 1106 32-33 0.12358970576601536 1106 34-35 0.18972455207803307 1106 36-37 0.11759472941703564 1106 38-39 0.14096102113031606 1106 40-41 0.15447445292221573 1106 42-43 0.11394057573572525 1106 44-45 0.10895077233290351 1106 46-47 0.06966731231666756 1106 48-49 0.07194866995337179 1106 50-51 0.08728740232501764 1106 52-53 0.08864643441053488 1106 54-55 0.06876624808666776 1106 56-57 0.03889961407661957 1106 58-59 0.0526995380404216 1106 60-61 0.16995479194216756 1106 62-63 0.10528735601199202 1106 64-65 0.09257289969043114 1106 66-67 0.1805642446996103 1106 68-69 0.14841843431978674 1106 70-71 0.1254026975139837 1106 72-73 0.19854632771392033 1106 74-75 0.15549105987128087 1106 76-77 0.12471890931761465 1106 78-79 0.14646821350678607 1106 80-81 0.15841117567389063 1106 82-83 0.19835357964571187 1106 84-85 0.15341268257225948 1106 86-87 0.09219909847332985 1106 88-89 0.15649745750877742 1106 90-91 0.15700725580764896 1106 92-93 0.16723517217781136 1106 94-95 0.19058274648112672 1106 96-97 0.1741515166651908 1106 98-99 0.27835880968626014 1106 100 0.24445509249260766 1107 1 0.04254537479658893 1107 2 0.039222241995496177 1107 3 0.03976480491871115 1107 4 0.028982278892243585 1107 5 0.028293853895547727 1107 6 0.0678808076219255 1107 7 0.07551018319375657 1107 8 -0.015953862748261827 1107 9 0.07531733598455759 1107 10-11 0.04274148639478881 1107 12-13 0.036154096300563765 1107 14-15 0.12262811608253799 1107 16-17 0.09837249757789834 1107 18-19 0.1036167743761709 1107 20-21 0.09390714557493851 1107 22-23 0.09992185996307512 1107 24-25 0.2137418921961185 1107 26-27 0.11270473652781021 1107 28-29 0.1048891482882226 1107 30-31 0.11231586542015748 1107 32-33 0.08938554100875962 1107 34-35 0.06609356356185714 1107 36-37 0.06384530282043954 1107 38-39 0.05432547528852183 1107 40-41 0.07755564638011947 1107 42-43 0.057562765305732455 1107 44-45 0.05011830857930022 1107 46-47 -0.02680956275011681 1107 48-49 -0.024209139007041358 1107 50-51 0.014101425842497406 1107 52-53 -0.046873504265725785 1107 54-55 -0.00786275649430479 1107 56-57 -0.01301625446119914 1107 58-59 0.03746334915235394 1107 60-61 0.06510837133539127 1107 62-63 0.062136707437403516 1107 64-65 0.08075997835917548 1107 66-67 0.03902093364874304 1107 68-69 -0.007904081362902105 1107 70-71 0.05790202009248446 1107 72-73 -0.012522449865365104 1107 74-75 0.059494638325730875 1107 76-77 0.06047182535929352 1107 78-79 0.11951048854895419 1107 80-81 0.08242105664010069 1107 82-83 0.1091155530852248 1107 84-85 0.045093679977775025 1107 86-87 0.09223572211649156 1107 88-89 0.05386172576722004 1107 90-91 0.017313728480274904 1107 92-93 0.008413788235237973 1107 94-95 -0.030379514245368 1107 96-97 -0.03414522199938119 1107 98-99 0.08904233203698908 1107 100 0.07007625150110641 1108 1 -0.02681747280333724 1108 2 -0.02997320979845597 1108 3 0.018712837820721973 1108 4 -0.005187657722792949 1108 5 0.015326371517197401 1108 6 0.012346657186640186 1108 7 0.015135983114625162 1108 8 0.03480787271225694 1108 9 0.04419039585589246 1108 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66-67 -0.30818659678616456 2214 68-69 -0.3053302944141194 2214 70-71 -0.2814367810085159 2214 72-73 -0.3591678943462142 2214 74-75 -0.44441462945619037 2214 76-77 -0.365679128849024 2214 78-79 -0.3721560288882486 2214 80-81 -0.35087379304719946 2214 82-83 -0.3617904945003616 2214 84-85 -0.3752672411437388 2214 86-87 -0.4116426288311956 2214 88-89 -0.37308358567116073 2214 90-91 -0.3315341849230933 2214 92-93 -0.36101946985132827 2214 94-95 -0.3188422154813111 2214 96-97 -0.3061737560779534 2214 98-99 -0.2862824000609976 2214 100 -0.29937602017517406 2215 1 -0.2078032938359229 2215 2 -0.22251000887231243 2215 3 -0.21969202318577175 2215 4 -0.11713783839061875 2215 5 -0.18576114352126183 2215 6 -0.18787817158649034 2215 7 -0.22676048215195976 2215 8 -0.16690150695017536 2215 9 -0.2350611791836812 2215 10-11 -0.22330071356865488 2215 12-13 -0.1817425158338608 2215 14-15 -0.3610405873709226 2215 16-17 -0.3466357194004104 2215 18-19 -0.2958859085569685 2215 20-21 -0.3928791270217502 2215 22-23 -0.36341159578334725 2215 24-25 -0.409909025192583 2215 26-27 -0.44206159080422225 2215 28-29 -0.5183684475132253 2215 30-31 -0.39401306493709853 2215 32-33 -0.36415034703001226 2215 34-35 -0.3870834315697138 2215 36-37 -0.42241734085148863 2215 38-39 -0.41908400315661964 2215 40-41 -0.3654841890633094 2215 42-43 -0.3096336092081913 2215 44-45 -0.3324075922210312 2215 46-47 -0.4215093404869066 2215 48-49 -0.3332389888812983 2215 50-51 -0.35002766626694637 2215 52-53 -0.3227202620021572 2215 54-55 -0.37241813826037884 2215 56-57 -0.33119234401414843 2215 58-59 -0.32947647953053405 2215 60-61 -0.2573193472157911 2215 62-63 -0.34791669666181946 2215 64-65 -0.3279306759860603 2215 66-67 -0.3502287840360765 2215 68-69 -0.28937523346755256 2215 70-71 -0.3367555042642394 2215 72-73 -0.24657039044605256 2215 74-75 -0.23569655143764123 2215 76-77 -0.24823053473715362 2215 78-79 -0.27146181076105336 2215 80-81 -0.29066126588109853 2215 82-83 -0.2855761912928827 2215 84-85 -0.26550716100011584 2215 86-87 -0.24985181603674533 2215 88-89 -0.3038546595301881 2215 90-91 -0.28746897855711495 2215 92-93 -0.31911448710071966 2215 94-95 -0.3398834366109895 2215 96-97 -0.39870175138811703 2215 98-99 -0.39074323362412855 2215 100 -0.40225393998912296 2216 1 -0.28590094525743126 2216 2 -0.2426983071829838 2216 3 -0.27195160291383047 2216 4 -0.2106528775335903 2216 5 -0.21681553165475975 2216 6 -0.1729004212774683 2216 7 -0.13669373307902788 2216 8 -0.22506302322541671 2216 9 -0.2603549575108275 2216 10-11 -0.30553062704207434 2216 12-13 -0.33313703581737286 2216 14-15 -0.4271635787182717 2216 16-17 -0.4514702888276787 2216 18-19 -0.3891813350093827 2216 20-21 -0.4523010470876798 2216 22-23 -0.38909783393744135 2216 24-25 -0.46700276231660354 2216 26-27 -0.48914502714538344 2216 28-29 -0.49094508533769954 2216 30-31 -0.34825987169440964 2216 32-33 -0.4385273556826661 2216 34-35 -0.4721561550967053 2216 36-37 -0.4833161871638083 2216 38-39 -0.4601577567618875 2216 40-41 -0.5161106826768247 2216 42-43 -0.5139657064475855 2216 44-45 -0.4333810162012526 2216 46-47 -0.35435997913543815 2216 48-49 -0.44615534652447764 2216 50-51 -0.39353631892454644 2216 52-53 -0.38857584502647313 2216 54-55 -0.4135557567193757 2216 56-57 -0.41723581331782356 2216 58-59 -0.33441117256720077 2216 60-61 -0.3645404020159546 2216 62-63 -0.42807429863956514 2216 64-65 -0.49773743329961206 2216 66-67 -0.49940760562651576 2216 68-69 -0.4287847925940511 2216 70-71 -0.4110192043054397 2216 72-73 -0.43473705711272004 2216 74-75 -0.48417697995020603 2216 76-77 -0.4613463155364954 2216 78-79 -0.4923606570733696 2216 80-81 -0.4864575163961433 2216 82-83 -0.4897049510786289 2216 84-85 -0.5584999504521093 2216 86-87 -0.44532853626748903 2216 88-89 -0.575993102051811 2216 90-91 -0.6238511376011999 2216 92-93 -0.6280186898201308 2216 94-95 -0.5607311910403254 2216 96-97 -0.6727520192084775 2216 98-99 -0.8006562950168004 2216 100 -0.7689012411840146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 1.0 4 13.0 5 17.0 6 36.0 7 93.0 8 190.0 9 415.0 10 772.0 11 1234.0 12 1977.0 13 2937.0 14 3707.0 15 4927.0 16 5960.0 17 8403.0 18 9478.0 19 5004.0 20 3557.0 21 3265.0 22 3375.0 23 3582.0 24 4386.0 25 5301.0 26 6820.0 27 8990.0 28 11749.0 29 15084.0 30 19607.0 31 25107.0 32 32445.0 33 42302.0 34 58260.0 35 87260.0 36 155111.0 37 350096.0 38 663994.0 39 341934.0 40 1430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.747712746489206 20.09011829151313 13.693647597966695 23.46852136403097 2 15.484818072149121 20.169930595839784 40.6144632601645 23.7307880718466 3 18.309154471346712 25.328427850702916 30.237440113174124 26.12497756477625 4 12.10122322604692 16.914975624425555 39.411025976611306 31.572775172916216 5 12.231083100781596 37.8992888178748 36.29681278380709 13.572815297536515 6 30.570910486678226 38.76801715352137 17.526028086244093 13.135044273556312 7 26.74949757203938 32.73643762163627 22.627364693873663 17.886700112450686 8 24.824414370438294 36.74274230181563 20.2430933142456 18.18975001350048 9 24.53066134131853 16.630259610721566 21.62415000201183 37.21492904594807 10-11 23.668248871848263 26.944316277971502 29.042213933094192 20.345220917086042 12-13 24.608460908033976 24.054757765132663 29.471608849508797 21.865172477324563 14-15 21.59611525375452 26.135925352474704 26.860953392415965 25.40700600135481 16-17 20.736543534397754 29.791788270205377 27.30772743336126 22.163940762035605 18-19 20.484869886743866 28.234274804957177 30.223166258650924 21.057689049648033 20-21 21.692578678418574 27.608972336284722 29.88914289707692 20.809306088219785 22-23 21.246409805037523 27.353513427660054 30.024168464521594 21.37590830278083 24-25 21.124003766409547 27.517899578118715 29.976495677285303 21.381600978186434 26-27 20.929525215286017 27.39928987579312 30.079713599648883 21.591471309271984 28-29 20.962805428139415 27.482311745297604 30.125120180599147 21.429762645963837 30-31 21.274946064352175 27.982211163024633 29.636890659537013 21.105952113086175 32-33 20.865769673045932 27.626734673564773 30.177416895556398 21.330078757832897 34-35 20.960960976859724 27.410092395919587 30.177813967179645 21.451132660041043 36-37 21.3446380506854 27.825402842397505 29.52868127749059 21.301277829426503 38-39 21.262814824854352 28.018962023011095 29.55761456310474 21.16060858902981 40-41 21.05802200466798 27.702014981259527 29.784260227183385 21.45570278688911 42-43 21.080770721315016 27.65918866090507 29.618181221371138 21.641859396408776 44-45 21.285474378821153 27.896108062777554 29.38735025105515 21.431067307346144 46-47 21.369574148625496 28.134933408441636 29.086555260722523 21.408937182210344 48-49 21.3663975756395 28.061369272374193 28.879575059243084 21.692658092743226 50-51 21.459629992778588 27.9704663420915 28.79131927311539 21.77858439201452 52-53 21.48469844792644 27.9603542514194 28.805693265877043 21.74925403477712 54-55 21.60175516246053 28.391838748684368 28.51355443693126 21.49285165192384 56-57 21.292489310831904 28.10049406298509 28.711004919452698 21.896011706730313 58-59 21.16095271776996 27.702284167748463 29.130789039552567 22.005974074929004 60-61 21.458571135116593 28.12942734859924 28.67841857490465 21.733582941379524 62-63 21.313719406955002 28.094432102870137 28.69811332741785 21.89373516275701 64-65 21.31824602346005 27.812987736310557 28.928414868902834 21.940351371326557 66-67 21.18737121643686 27.986481564229248 28.70822541808995 22.117921801243945 68-69 21.703986635066748 27.998010401713024 28.609369800831047 21.68863316238918 70-71 21.667860087805796 27.92126332341095 28.595120517887416 21.815756070895834 72-73 21.431988147106544 28.042723994829583 28.71118313721016 21.814104720853717 74-75 21.484594527128102 28.116837685641155 28.531222180132577 21.86734560709817 76-77 21.546673796310145 28.153259098567762 28.38284597190585 21.91722113321624 78-79 21.673731012034978 28.215062462013478 28.468050028906816 21.64315649704473 80-81 21.595587316579383 27.930335636701702 28.621107903948907 21.852969142770014 82-83 21.399980358174773 28.043629205752886 28.727334141065896 21.82905629500645 84-85 21.399486877576994 28.009670547027042 28.602154351799104 21.988688223596856 86-87 21.571005216212747 28.100999432185926 28.468185867120916 21.85980948448041 88-89 21.28125481459284 28.003286762047004 28.56229573552154 22.15316268783861 90-91 21.206554880873533 27.90231952608838 28.680701041036304 22.210424552001783 92-93 21.348162023193364 28.12155869073536 28.476516505578097 22.053762780493177 94-95 21.17988043848575 27.81950727399165 28.63382585462817 22.366786432894425 96-97 21.097348880918034 27.843050573769407 28.6861673262478 22.37343321906476 98-99 21.09108486142057 27.961832386405 28.551193029110934 22.3958897230635 100 21.29373346858475 27.864633518774607 28.39469766437177 22.446935348268873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 9.0 2 48.0 3 88.5 4 61.0 5 46.5 6 131.5 7 296.5 8 487.0 9 618.0 10 566.0 11 461.5 12 491.5 13 627.0 14 754.0 15 885.0 16 1048.5 17 1258.0 18 1529.0 19 1796.5 20 2187.0 21 2815.5 22 3696.5 23 4958.5 24 6724.5 25 8972.5 26 11890.5 27 15680.5 28 20631.5 29 26201.5 30 31851.0 31 37980.5 32 44244.5 33 50347.0 34 55873.0 35 60339.5 36 64000.5 37 67705.0 38 70587.5 39 71177.5 40 71266.0 41 71319.5 42 70356.0 43 69205.5 44 67749.5 45 66577.0 46 65277.5 47 62216.5 48 59642.0 49 58083.5 50 56371.5 51 53946.0 52 50068.5 53 47132.5 54 45461.0 55 43127.5 56 39576.5 57 36104.5 58 32692.5 59 28895.5 60 25305.5 61 21394.0 62 17682.5 63 14384.0 64 11416.5 65 8887.0 66 6758.5 67 5008.5 68 3783.5 69 2970.5 70 2170.0 71 1519.5 72 1160.5 73 827.5 74 546.0 75 357.0 76 210.5 77 137.0 78 81.5 79 42.0 80 22.0 81 9.0 82 5.0 83 4.0 84 2.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045795593881496885 2 0.2460255777656833 3 0.003017744336699795 4 0.0027530299211998128 5 5.2942883099996405E-5 6 1.588286492999892E-4 7 0.0 8 3.176572985999784E-4 9 0.0 10-11 4.5001450634996943E-4 12-13 0.0 14-15 2.3824297394998381E-4 16-17 2.911858570499802E-4 18-19 6.353145971999568E-4 20-21 0.0 22-23 1.588286492999892E-4 24-25 0.0014029864021499045 26-27 1.853000908499874E-4 28-29 2.1177153239998562E-4 30-31 1.588286492999892E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.94143246499946E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3824297394998381E-4 70-71 2.6471441549998202E-5 72-73 3.441287401499766E-4 74-75 1.3235720774999098E-4 76-77 2.6471441549998202E-5 78-79 0.0 80-81 0.0 82-83 7.94143246499946E-5 84-85 0.0 86-87 2.911858570499802E-4 88-89 0.0020647724408998595 90-91 0.0019588866746998667 92-93 6.353145971999568E-4 94-95 5.029573894499657E-4 96-97 1.588286492999892E-4 98-99 7.94143246499946E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1888828.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.15226952521232 #Duplication Level Percentage of deduplicated Percentage of total 1 79.76668440197315 55.9581394329977 2 13.037468282432313 18.29215977751197 3 3.665506200591802 7.714307367907593 4 1.4585032753042793 4.092692594902029 5 0.6731859982140135 2.3612762793654287 6 0.4012961738855047 1.689110240991144 7 0.2351569008730194 1.1547753203530386 8 0.1544366292006993 0.8667264029002187 9 0.11322289406449457 0.7148558682753287 >10 0.4734710223436553 5.506636618230503 >50 0.01668389140050632 0.7865740870393823 >100 0.004082175132436 0.474049142545263 >500 7.553864604797938E-5 0.045202239609297264 >1k 2.2661593814393813E-4 0.3434946273710022 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2811 0.14882244439408987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2461 0.13029243530909113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.058857661999928E-4 0.0 5 0.0 0.0 0.0 1.058857661999928E-4 0.0 6 0.0 0.0 0.0 1.058857661999928E-4 0.0 7 0.0 0.0 0.0 1.058857661999928E-4 0.0 8 0.0 0.0 0.0 1.058857661999928E-4 0.0 9 0.0 0.0 0.0 1.058857661999928E-4 0.0 10-11 0.0 0.0 0.0 1.058857661999928E-4 0.0 12-13 0.0 0.0 0.0 2.1177153239998562E-4 0.0 14-15 0.0 0.0 0.0 3.706001816999748E-4 0.0 16-17 0.0 0.0 0.0 5.29428830999964E-4 0.0 18-19 0.0 0.0 0.0 5.29428830999964E-4 0.0 20-21 0.0 0.0 0.0 6.353145971999568E-4 0.0 22-23 0.0 0.0 0.0 7.94143246499946E-4 0.0 24-25 0.0 0.0 0.0 9.529718957999351E-4 0.0 26-27 0.0 0.0 0.0 0.001058857661999928 0.0 28-29 0.0 0.0 0.0 0.0014294578436999028 0.0 30-31 0.0 0.0 0.0 0.0031501015444497854 0.0 32-33 0.0 0.0 0.0 0.007703189491049476 0.0 34-35 0.0 0.0 0.0 0.014241635553899032 0.0 36-37 0.0 0.0 0.0 0.025280226680248284 0.0 38-39 0.0 0.0 0.0 0.04481615054414695 0.0 40-41 0.0 0.0 0.0 0.0720023210159951 0.0 42-43 0.0 0.0 0.0 0.09908260572164326 0.0 44-45 0.0 0.0 0.0 0.1296835921534412 0.0 46-47 0.0 0.0 0.0 0.1689936828551885 0.0 48-49 0.0 0.0 0.0 0.21396866204863546 0.0 50-51 0.0 0.0 0.0 0.26638211631763187 0.0 52-53 0.0 0.0 0.0 0.31876909914507834 0.0 54-55 0.0 0.0 0.0 0.3730355543225746 0.0 56-57 0.0 0.0 0.0 0.43902885810672015 0.0 58-59 0.0 0.0 0.0 0.5173049107700648 0.0 60-61 0.0 0.0 0.0 0.6007428945356592 0.0 62-63 0.0 0.0 0.0 0.6873309798457032 0.0 64-65 0.0 0.0 0.0 0.779186882024197 0.0 66-67 0.0 0.0 0.0 0.8842520335361399 0.0 68-69 0.0 0.0 0.0 1.0025793772646319 0.0 70-71 0.0 0.0 0.0 1.1366837001569228 0.0 72-73 0.0 0.0 0.0 1.2824089858896628 0.0 74-75 0.0 0.0 0.0 1.4325020594781526 0.0 76-77 0.0 0.0 0.0 1.588921807597092 0.0 78-79 0.0 0.0 0.0 1.7708070824871296 0.0 80-81 0.0 0.0 0.0 1.9809109140694652 0.0 82-83 0.0 0.0 0.0 2.20332396597255 0.0 84-85 0.0 0.0 0.0 2.4184309000078357 0.0 86-87 0.0 0.0 0.0 2.6406851232616204 0.0 88 0.0 0.0 0.0 2.822490983827008 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2545 0.0 48.90498 1 GTATCAA 4710 0.0 43.441593 1 TCAACGC 5395 0.0 37.457924 4 ATCAACG 5395 0.0 37.371803 3 CAACGCA 5460 0.0 37.18611 5 AACGCAG 5710 0.0 35.558 6 TATCAAC 5975 0.0 33.98867 2 ACGCAGA 6585 0.0 30.54764 7 CGCAGAG 6620 0.0 30.315138 8 GTGGTAT 1405 0.0 26.508654 1 GCAGAGT 7610 0.0 26.062582 9 GTACATG 4755 0.0 25.481178 1 TGGTATC 1410 0.0 24.060696 2 TACATGG 4775 0.0 23.978815 2 CACCGTT 165 2.6657835E-6 22.787449 7 TACGACC 125 2.4254131E-4 22.55838 4 ACATGGG 4990 0.0 22.415815 3 GAGTACT 5260 0.0 21.622309 12-13 AGAGTAC 6945 0.0 21.181746 10-11 TACGGGT 135 4.080368E-4 20.88739 4 >>END_MODULE