FastQCFastQC Report
Wed 25 May 2016
SRR1033046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070232
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.037.788661
GTATCAA40800.034.5721661
TCAACGC41950.032.722434
CAACGCA43800.031.3413415
ATCAACG43900.031.2689293
AACGCAG47200.029.1752136
TATCAAC51200.026.9942992
GTACATG93250.026.8747161
TACATGG93750.025.12242
ACGCAGA56950.023.5205067
CGCAGAG57500.023.1547058
ACATGGG100600.022.6173673
CGCTATA1805.6588615E-620.8935472
GAGTACA68850.020.0091761
AGTACAT67450.019.1666152
CATGGGG72150.018.504474
GCAGAGT68800.018.2602759
TACACCG3602.1918822E-916.976565
CCGGTCG3252.1325286E-715.9255269
ACACCGT5350.015.812746
Produced by FastQC (version 0.11.5)