FastQCFastQC Report
Wed 25 May 2016
SRR1033046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070232
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37930.12354115259042313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.10331466807720069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38600.050.063251
GTATCAA70400.043.4504741
TCAACGC83650.035.6771974
ATCAACG84300.035.401533
CAACGCA85700.034.6592565
AACGCAG89450.033.2062386
TATCAAC91250.033.0418172
ACGCAGA104700.028.2349417
CGCAGAG106550.027.5682728
GTACATG91700.025.4424151
TACATGG92950.024.1760792
GCAGAGT121000.023.8876119
ACATGGG95900.022.1511233
GAGTACT71400.021.12883812-13
AGAGTAC109200.020.31415610-11
GTACCGT1405.2144553E-420.1421666
CAGAGTA115500.020.01992810-11
GAGTACA70500.019.9227681
GTGGTAT22550.019.2293151
GTACTTT79500.018.828314-15
Produced by FastQC (version 0.11.5)