##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033046_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3070232 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67092486821843 34.0 31.0 34.0 31.0 34.0 2 32.66236688302382 34.0 31.0 34.0 31.0 34.0 3 32.77341223725113 34.0 31.0 34.0 31.0 34.0 4 36.27938247011952 37.0 37.0 37.0 35.0 37.0 5 36.26035556922083 37.0 37.0 37.0 35.0 37.0 6 36.32503244054521 37.0 37.0 37.0 35.0 37.0 7 36.2685634180088 37.0 37.0 37.0 35.0 37.0 8 36.27237192498808 37.0 37.0 37.0 35.0 37.0 9 38.06557419765021 39.0 39.0 39.0 35.0 39.0 10-11 38.0275848860933 39.0 38.5 39.0 35.0 39.0 12-13 38.01080227813403 39.0 38.0 39.0 35.0 39.0 14-15 39.500155362852055 41.0 39.0 41.0 36.0 41.0 16-17 39.490322392574896 41.0 39.0 41.0 36.0 41.0 18-19 39.49509271612048 41.0 39.5 41.0 36.5 41.0 20-21 39.48247510285867 41.0 39.0 41.0 36.0 41.0 22-23 39.4179438231378 41.0 39.0 41.0 36.0 41.0 24-25 39.36846922317271 41.0 39.0 41.0 36.0 41.0 26-27 39.26422986927372 41.0 39.0 41.0 36.0 41.0 28-29 39.21080768489156 41.0 39.0 41.0 36.0 41.0 30-31 39.21698897672879 41.0 39.0 41.0 36.0 41.0 32-33 39.190840138465106 41.0 39.0 41.0 35.0 41.0 34-35 39.12335370747227 41.0 39.0 41.0 35.0 41.0 36-37 39.04250997970186 41.0 39.0 41.0 35.0 41.0 38-39 38.957991122494974 41.0 39.0 41.0 35.0 41.0 40-41 38.8600579695606 41.0 39.0 41.0 35.0 41.0 42-43 38.74810258638435 41.0 38.5 41.0 35.0 41.0 44-45 38.59306251123694 41.0 38.0 41.0 35.0 41.0 46-47 38.41712238684243 40.0 38.0 41.0 34.5 41.0 48-49 38.227371579737294 40.0 38.0 41.0 34.0 41.0 50-51 38.08688431362842 40.0 38.0 41.0 34.0 41.0 52-53 37.94147461820475 40.0 37.5 41.0 33.5 41.0 54-55 37.7825302778422 40.0 37.0 41.0 33.0 41.0 56-57 37.6192681855964 40.0 37.0 41.0 33.0 41.0 58-59 37.42208357544316 40.0 36.0 41.0 33.0 41.0 60-61 37.21633707159589 39.5 36.0 41.0 33.0 41.0 62-63 36.96979544216854 39.0 35.0 41.0 32.5 41.0 64-65 36.68987767048223 39.0 35.0 41.0 32.0 41.0 66-67 36.39469330005029 38.0 35.0 40.5 32.0 41.0 68-69 36.08053023354587 37.0 35.0 40.0 31.5 41.0 70-71 35.74352589641434 37.0 35.0 39.5 31.0 41.0 72-73 35.39108656935372 36.0 35.0 39.0 31.0 41.0 74-75 35.043072477910464 36.0 35.0 39.0 31.0 41.0 76-77 34.69264553949017 35.5 35.0 38.0 31.0 40.0 78-79 34.34417447932274 35.0 34.0 37.0 30.0 39.0 80-81 34.028918661521345 35.0 34.0 37.0 30.0 39.0 82-83 33.7493767571962 35.0 34.0 36.0 30.0 38.5 84-85 33.54724708100235 35.0 34.0 36.0 30.0 37.0 86-87 33.35809427430891 35.0 34.0 36.0 30.0 37.0 88-89 33.16740966155001 35.0 34.0 35.0 30.0 36.5 90-91 33.001199257906244 35.0 34.0 35.0 30.0 36.0 92-93 32.84258192866207 35.0 34.0 35.0 29.5 36.0 94-95 32.69490058080301 35.0 34.0 35.0 29.0 36.0 96-97 32.555558993587454 35.0 34.0 35.0 29.0 36.0 98-99 32.43618560421493 35.0 34.0 35.0 29.0 35.0 100 32.33618827502286 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1205368476288271 1101 2 0.15097302230423537 1101 3 0.16204506711595457 1101 4 0.12259104796074638 1101 5 0.1425708716563321 1101 6 0.13616256003466987 1101 7 0.19085534207630417 1101 8 0.1852010386637417 1101 9 0.23226880374196668 1101 10-11 0.2716212357147114 1101 12-13 0.24051803166729968 1101 14-15 0.3389415099902138 1101 16-17 0.315782652150844 1101 18-19 0.315032410978894 1101 20-21 0.3175134865008431 1101 22-23 0.35420502990017866 1101 24-25 0.35446793266358156 1101 26-27 0.366385165341633 1101 28-29 0.3355242205938964 1101 30-31 0.3635726299996662 1101 32-33 0.3639459784110244 1101 34-35 0.3626766521217277 1101 36-37 0.30942630070994426 1101 38-39 0.25736908392541835 1101 40-41 0.2834478990172755 1101 42-43 0.27362342723601074 1101 44-45 0.3050328242174416 1101 46-47 0.2734056438923176 1101 48-49 0.2519298839467794 1101 50-51 0.3195940890842337 1101 52-53 0.29439426033662386 1101 54-55 0.3304378438883404 1101 56-57 0.3169088400896314 1101 58-59 0.3014841038700524 1101 60-61 0.2950027398629871 1101 62-63 0.319326747155408 1101 64-65 0.3444673992401093 1101 66-67 0.33030950573853346 1101 68-69 0.29258063632860853 1101 70-71 0.25704807824256193 1101 72-73 0.2934874425223839 1101 74-75 0.29400691054383543 1101 76-77 0.2713623659567759 1101 78-79 0.2604198175252179 1101 80-81 0.2881026316265647 1101 82-83 0.25189928264735784 1101 84-85 0.26641980670493126 1101 86-87 0.2797439863293647 1101 88-89 0.3228290755405041 1101 90-91 0.2586578423358503 1101 92-93 0.23547654690419506 1101 94-95 0.1956229610070821 1101 96-97 0.26834722190921667 1101 98-99 0.24195711291794453 1101 100 0.2565975673057679 1102 1 0.13137811702110724 1102 2 0.15253825191766168 1102 3 0.180606410982314 1102 4 0.12813307799113716 1102 5 0.1295395525920071 1102 6 0.15960142535530508 1102 7 0.17505136344495753 1102 8 0.19120880577239774 1102 9 0.22181241429630205 1102 10-11 0.23161397666293482 1102 12-13 0.24418661566213018 1102 14-15 0.40042376538560376 1102 16-17 0.3674004197133982 1102 18-19 0.3263877946824394 1102 20-21 0.33092187389060257 1102 22-23 0.32471424125425585 1102 24-25 0.3784146081801367 1102 26-27 0.3738843490957464 1102 28-29 0.3469548699528673 1102 30-31 0.33696378138987626 1102 32-33 0.36337693606863297 1102 34-35 0.33683117706222987 1102 36-37 0.35237237388197684 1102 38-39 0.34744088615468627 1102 40-41 0.37883091842758887 1102 42-43 0.35806326985898096 1102 44-45 0.3405012024459424 1102 46-47 0.3730911123911511 1102 48-49 0.39123403130914625 1102 50-51 0.38960427763100114 1102 52-53 0.37206175629596316 1102 54-55 0.399353144645076 1102 56-57 0.41304182995283867 1102 58-59 0.42789734948518543 1102 60-61 0.4315149589927145 1102 62-63 0.403130148220157 1102 64-65 0.3881367737161838 1102 66-67 0.47306929504294004 1102 68-69 0.4675202757945982 1102 70-71 0.4604236908107069 1102 72-73 0.3991720990511567 1102 74-75 0.4262742124899077 1102 76-77 0.39491230978984504 1102 78-79 0.46777357356099003 1102 80-81 0.4217708724582465 1102 82-83 0.44329707605349 1102 84-85 0.437890127039509 1102 86-87 0.44914779024353635 1102 88-89 0.4608490197880073 1102 90-91 0.37795686637107906 1102 92-93 0.47190951508124357 1102 94-95 0.48376655910667665 1102 96-97 0.45143917888862006 1102 98-99 0.41564744117706454 1102 100 0.47003276637219216 1103 1 0.01604179599729605 1103 2 0.01933081999558084 1103 3 0.023918806957169636 1103 4 0.06073822478369806 1103 5 0.06827425391117714 1103 6 0.04805348913863128 1103 7 0.07635864606653087 1103 8 0.09475958684845409 1103 9 0.09964267550077466 1103 10-11 0.13500921887506934 1103 12-13 0.12579423427285263 1103 14-15 0.15377620027773986 1103 16-17 0.21979477301199069 1103 18-19 0.22788506366784844 1103 20-21 0.20912654161085698 1103 22-23 0.16729956393069756 1103 24-25 0.12426399950960842 1103 26-27 0.1151847800255581 1103 28-29 0.195584363566212 1103 30-31 0.20120702442875427 1103 32-33 0.08011818997442788 1103 34-35 0.07628635624384827 1103 36-37 0.0958227278930508 1103 38-39 0.11444114745157208 1103 40-41 0.09952197728492251 1103 42-43 0.08595522149469303 1103 44-45 0.1340191071641641 1103 46-47 0.15886956707630873 1103 48-49 0.11735718784279214 1103 50-51 0.12763251113800322 1103 52-53 0.15119079326989038 1103 54-55 0.08431458444974993 1103 56-57 0.06606077523760234 1103 58-59 0.0286282456158915 1103 60-61 0.08063923633038428 1103 62-63 0.007094593359155965 1103 64-65 -0.02342525656904826 1103 66-67 0.018717168497950354 1103 68-69 -0.02484424680925912 1103 70-71 0.05804538500596834 1103 72-73 0.09154164174799462 1103 74-75 0.05639638768989386 1103 76-77 0.010351973313390772 1103 78-79 0.020814216569789323 1103 80-81 0.0904518776867036 1103 82-83 0.01829852536904042 1103 84-85 0.06260330959361227 1103 86-87 0.07392125592248533 1103 88-89 0.011589977109231597 1103 90-91 0.042427504918507 1103 92-93 0.048823220733233086 1103 94-95 3.600668451824163E-4 1103 96-97 0.04080576078082743 1103 98-99 0.029923451350001073 1103 100 0.013813438300083192 1104 1 0.08947845558682133 1104 2 0.09453332732547892 1104 3 0.1078904983938287 1104 4 0.0508102094161913 1104 5 0.05499046056368684 1104 6 0.09730533425035048 1104 7 0.12444256073642634 1104 8 0.13571419204509994 1104 9 0.09425070300051885 1104 10-11 0.11959550944225583 1104 12-13 0.1447418634306743 1104 14-15 0.2004788592606488 1104 16-17 0.20247367302210506 1104 18-19 0.1759467358951241 1104 20-21 0.1353897101479049 1104 22-23 0.17553664814867886 1104 24-25 0.16909050845714546 1104 26-27 0.2089785319212254 1104 28-29 0.17726265696424548 1104 30-31 0.16383820428923457 1104 32-33 0.11307653594552391 1104 34-35 0.20394351932139188 1104 36-37 0.2249657872399311 1104 38-39 0.26359077842923284 1104 40-41 0.21611746813518806 1104 42-43 0.27766253117015793 1104 44-45 0.3281789494450251 1104 46-47 0.323519551911005 1104 48-49 0.21860306793580975 1104 50-51 0.2177935666430031 1104 52-53 0.2917444278872168 1104 54-55 0.2794614856730888 1104 56-57 0.3388500786447395 1104 58-59 0.2972743885741309 1104 60-61 0.3556137586233703 1104 62-63 0.2735351959271455 1104 64-65 0.29229620738808393 1104 66-67 0.27366732520606263 1104 68-69 0.3426418516640979 1104 70-71 0.3044249219587556 1104 72-73 0.31219395192896116 1104 74-75 0.2270202882055159 1104 76-77 0.22607697584093955 1104 78-79 0.2625513513387716 1104 80-81 0.2393251968657566 1104 82-83 0.2196257925816596 1104 84-85 0.1987796315024184 1104 86-87 0.22883076962180837 1104 88-89 0.26860463690096026 1104 90-91 0.23713107381952625 1104 92-93 0.25891967205262034 1104 94-95 0.2550817330063424 1104 96-97 0.2738965503875477 1104 98-99 0.2956058899305276 1104 100 0.2645787805303925 1105 1 0.03393394333262734 1105 2 0.07661857386234061 1105 3 0.110598819618275 1105 4 0.04986997886305744 1105 5 0.04207464151883755 1105 6 0.10065222953272723 1105 7 0.07666148934245598 1105 8 0.08812182548498981 1105 9 0.104063354361557 1105 10-11 0.10014099040709112 1105 12-13 0.14440203973601484 1105 14-15 0.17144582622761817 1105 16-17 0.16818557847594917 1105 18-19 0.1594148168793339 1105 20-21 0.12066705200589922 1105 22-23 0.18010227829495307 1105 24-25 0.17331776797472287 1105 26-27 0.17314912186601106 1105 28-29 0.12426546622512546 1105 30-31 0.09248975906982082 1105 32-33 0.12551255260734706 1105 34-35 0.1428642787905332 1105 36-37 0.16957617181805063 1105 38-39 0.21556045778601174 1105 40-41 0.12112202107522307 1105 42-43 0.1148749694148492 1105 44-45 0.07921498548105887 1105 46-47 0.15548613139371525 1105 48-49 0.21984117701261852 1105 50-51 0.22068284301615648 1105 52-53 0.23609946033901963 1105 54-55 0.26179036293744673 1105 56-57 0.21397581728660953 1105 58-59 0.2493341580210071 1105 60-61 0.2724481091028892 1105 62-63 0.23990233726202348 1105 64-65 0.28767758876946914 1105 66-67 0.2331329844136576 1105 68-69 0.3057422100870397 1105 70-71 0.26896333133587547 1105 72-73 0.2141516192737285 1105 74-75 0.17923156399743334 1105 76-77 0.18815360085304889 1105 78-79 0.1577619490332438 1105 80-81 0.16307139467647147 1105 82-83 0.16982834997775598 1105 84-85 0.18090595137067567 1105 86-87 0.13278262470269198 1105 88-89 0.1099834996346587 1105 90-91 0.150091066069848 1105 92-93 0.19835907920170115 1105 94-95 0.20810837254911263 1105 96-97 0.2546043872566699 1105 98-99 0.2213207983873673 1105 100 0.22370306610628887 1106 1 0.01134009974697392 1106 2 0.07042340076779396 1106 3 0.059777728907924654 1106 4 0.06908502581041631 1106 5 0.09272796352507129 1106 6 0.09980474197364231 1106 7 0.1169708715632467 1106 8 0.08187813423889168 1106 9 0.0884208048721149 1106 10-11 0.09876324220699928 1106 12-13 0.06085392218452057 1106 14-15 0.11914363064183675 1106 16-17 0.13476767918178467 1106 18-19 0.15125637815999227 1106 20-21 0.13806940690223257 1106 22-23 0.1589853569854469 1106 24-25 0.10172519218137666 1106 26-27 0.11237014528299483 1106 28-29 0.09946000356458029 1106 30-31 0.1264572519082776 1106 32-33 0.10488629666572535 1106 34-35 0.08936948473242978 1106 36-37 0.07457512152090828 1106 38-39 0.09872842353702538 1106 40-41 0.07740010258200414 1106 42-43 0.04748911254897337 1106 44-45 0.04623509727728958 1106 46-47 0.1259495448036816 1106 48-49 0.1203531271634759 1106 50-51 0.13885540033170685 1106 52-53 0.1509375100952255 1106 54-55 0.15973559133524873 1106 56-57 0.07685552759048875 1106 58-59 0.14493513247368384 1106 60-61 0.135982628559411 1106 62-63 0.1260976218925549 1106 64-65 0.1110107300130494 1106 66-67 0.11166258699237375 1106 68-69 0.15235992794934816 1106 70-71 0.07042468504807431 1106 72-73 0.09143299480980005 1106 74-75 0.18072888806337062 1106 76-77 0.14862115968362843 1106 78-79 0.09808629566475702 1106 80-81 0.08563270693446157 1106 82-83 0.11942607687447548 1106 84-85 0.11171613943892567 1106 86-87 0.10133491555878749 1106 88-89 0.1415276409284374 1106 90-91 0.16583209253546727 1106 92-93 0.21787276373705566 1106 94-95 0.1916934363941678 1106 96-97 0.13847698156499177 1106 98-99 0.11205578332071298 1106 100 0.09999218967514878 1107 1 -0.015229068909391685 1107 2 -0.016775971990476535 1107 3 -0.011287907965943589 1107 4 0.0018089152767970518 1107 5 -0.044198853542432914 1107 6 0.010001766123153288 1107 7 0.020601370128183305 1107 8 -0.009718050513598087 1107 9 -0.005464031700903149 1107 10-11 0.025034222697939867 1107 12-13 0.02047878337890552 1107 14-15 0.0471484839602212 1107 16-17 0.009233610163910555 1107 18-19 0.05298093815061833 1107 20-21 0.04181307860788053 1107 22-23 0.10296278976450424 1107 24-25 0.053217241679547556 1107 26-27 0.021562003912350747 1107 28-29 0.0757591443001715 1107 30-31 0.04565739452772277 1107 32-33 0.02612719225906801 1107 34-35 -0.004055741170382987 1107 36-37 0.01496236701437681 1107 38-39 0.031369295715236944 1107 40-41 0.02062310688542368 1107 42-43 0.04273269201319607 1107 44-45 0.03551810640564668 1107 46-47 -0.025280503552110645 1107 48-49 0.00939582075518075 1107 50-51 0.03723949010390726 1107 52-53 0.06196138111332061 1107 54-55 0.10111021926524444 1107 56-57 0.09852543453308016 1107 58-59 0.09010420924338547 1107 60-61 0.06584171203435574 1107 62-63 0.10297021164168996 1107 64-65 0.07876339171050972 1107 66-67 0.09960317286736853 1107 68-69 0.06429650132207598 1107 70-71 0.020431115340159067 1107 72-73 0.020364963206461084 1107 74-75 0.06559322220439867 1107 76-77 0.05402548758063119 1107 78-79 0.031070855868264857 1107 80-81 0.018862726908281502 1107 82-83 0.09443915919285928 1107 84-85 0.11734960746840528 1107 86-87 0.10245990624595436 1107 88-89 0.08648354242878753 1107 90-91 0.018913843277836406 1107 92-93 0.03081401481468049 1107 94-95 0.004811720064942904 1107 96-97 0.03584672602074335 1107 98-99 0.02530222936480442 1107 100 0.05859246143259611 1108 1 -0.024480663258565016 1108 2 0.0394923771148612 1108 3 0.03393676591678485 1108 4 0.03238731044513088 1108 5 -0.05460210901023288 1108 6 0.020055834906379744 1108 7 0.023163781646587722 1108 8 0.01947484393377863 1108 9 -0.008849082925870277 1108 10-11 -0.0010013416039456047 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2214 72-73 -0.2573683489891536 2214 74-75 -0.3060436053676554 2214 76-77 -0.23525467115175047 2214 78-79 -0.24995174028573786 2214 80-81 -0.22486667122493031 2214 82-83 -0.24828145868310258 2214 84-85 -0.2387911011189061 2214 86-87 -0.22989510633847487 2214 88-89 -0.17370827297350644 2214 90-91 -0.118872486232938 2214 92-93 -0.22505503283777273 2214 94-95 -0.22812306369592505 2214 96-97 -0.19977442581631522 2214 98-99 -0.1952700703467798 2214 100 -0.1347541780605752 2215 1 -0.23401245501678147 2215 2 -0.21451080928586208 2215 3 -0.2687123553022488 2215 4 -0.12112531202812704 2215 5 -0.19536063557175964 2215 6 -0.19924818295086055 2215 7 -0.27877479439481334 2215 8 -0.2015826173063715 2215 9 -0.3075327322505288 2215 10-11 -0.2835554951752428 2215 12-13 -0.2821215252294209 2215 14-15 -0.37060745880582857 2215 16-17 -0.38770407979622945 2215 18-19 -0.3771092148515791 2215 20-21 -0.3832342342757329 2215 22-23 -0.4382603899976658 2215 24-25 -0.44735738870996045 2215 26-27 -0.46115150676590844 2215 28-29 -0.4854726897639807 2215 30-31 -0.5119767214564348 2215 32-33 -0.4304751508966973 2215 34-35 -0.4018305561393234 2215 36-37 -0.4352193702125433 2215 38-39 -0.5090019250942106 2215 40-41 -0.45742693887009267 2215 42-43 -0.4192844300179175 2215 44-45 -0.414577196817298 2215 46-47 -0.4741690790636639 2215 48-49 -0.47788459794048066 2215 50-51 -0.42262125926489347 2215 52-53 -0.2784005032663259 2215 54-55 -0.3558901096511775 2215 56-57 -0.33617429840921176 2215 58-59 -0.3580613575051501 2215 60-61 -0.3660683828966498 2215 62-63 -0.3484376814804051 2215 64-65 -0.3779502254918157 2215 66-67 -0.3802928228866236 2215 68-69 -0.34394952068915785 2215 70-71 -0.29602164403817 2215 72-73 -0.2116563746237219 2215 74-75 -0.2711873743428441 2215 76-77 -0.28110589366278305 2215 78-79 -0.2884350873650021 2215 80-81 -0.3479756059962611 2215 82-83 -0.33666760564621967 2215 84-85 -0.3918566262454064 2215 86-87 -0.34114260697455734 2215 88-89 -0.3203956542925184 2215 90-91 -0.2810862813117154 2215 92-93 -0.24175760237643118 2215 94-95 -0.2503992770190351 2215 96-97 -0.25760867029551804 2215 98-99 -0.28116872631807155 2215 100 -0.34912312054702 2216 1 -0.2750810720987076 2216 2 -0.24061824307672453 2216 3 -0.2716168706669677 2216 4 -0.23932375772148617 2216 5 -0.2346691951850488 2216 6 -0.24158789576419082 2216 7 -0.22264428348227483 2216 8 -0.2748573605584923 2216 9 -0.32758316212070326 2216 10-11 -0.36316876743473614 2216 12-13 -0.4119899418264268 2216 14-15 -0.49898970013680355 2216 16-17 -0.4331623452974398 2216 18-19 -0.4660724952915203 2216 20-21 -0.49446505749334335 2216 22-23 -0.47199136296460154 2216 24-25 -0.4361828522252864 2216 26-27 -0.5337757820723965 2216 28-29 -0.514426366499535 2216 30-31 -0.46443522151007954 2216 32-33 -0.4730876671323401 2216 34-35 -0.4966168548836407 2216 36-37 -0.5001302384595334 2216 38-39 -0.5292959398968264 2216 40-41 -0.5314260190910431 2216 42-43 -0.4897239375411786 2216 44-45 -0.5454451307789796 2216 46-47 -0.5392193293809129 2216 48-49 -0.590766448679851 2216 50-51 -0.5667780293718394 2216 52-53 -0.5987429937011939 2216 54-55 -0.57320858877722 2216 56-57 -0.5594585970948813 2216 58-59 -0.5490654781533024 2216 60-61 -0.5761613927733791 2216 62-63 -0.5987995482720265 2216 64-65 -0.6800433554605618 2216 66-67 -0.6999434494678098 2216 68-69 -0.7105766588956897 2216 70-71 -0.6644386798732 2216 72-73 -0.6950184376436539 2216 74-75 -0.6617589004381017 2216 76-77 -0.6871469828315497 2216 78-79 -0.7213909768069158 2216 80-81 -0.7191331825920386 2216 82-83 -0.7428265744171085 2216 84-85 -0.792986229885436 2216 86-87 -0.8313428754043528 2216 88-89 -0.9252155970857174 2216 90-91 -0.9281692886644635 2216 92-93 -0.8692989253296872 2216 94-95 -0.8557887060985223 2216 96-97 -0.9011724952159845 2216 98-99 -1.0199899295654689 2216 100 -1.0512363743392648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 10.0 4 13.0 5 28.0 6 51.0 7 163.0 8 338.0 9 642.0 10 1170.0 11 1748.0 12 2840.0 13 4251.0 14 4950.0 15 6623.0 16 8120.0 17 11504.0 18 13489.0 19 7326.0 20 5433.0 21 5284.0 22 5590.0 23 6246.0 24 7242.0 25 8958.0 26 11503.0 27 15349.0 28 20158.0 29 26066.0 30 33380.0 31 42784.0 32 55447.0 33 72206.0 34 100941.0 35 153573.0 36 285245.0 37 631716.0 38 1066977.0 39 451569.0 40 1285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.205448883802596 19.30206216607599 13.406327921032634 24.08616102908878 2 15.921136446967473 19.995794710019464 39.57997380835697 24.503095034656095 3 18.46481867998718 24.972574647769882 29.838652939659017 26.723953732583922 4 12.60931522555197 16.520218166302133 38.12477301486742 32.74569359327847 5 13.004100026577866 37.641748615084765 35.51929761476687 13.834853743570502 6 31.47526406816428 37.61802861674858 17.38202023952603 13.524687075561115 7 27.88111777872161 32.26427188564252 22.1149085801985 17.739701755437373 8 25.817374415637673 35.777549998746025 19.940740538077478 18.464335047538828 9 25.61282013867356 16.055073362534166 20.680912712785222 37.651193786007056 10-11 24.536836408767915 26.221131311098095 28.463269609814162 20.77876267031983 12-13 25.29419600864039 23.527163419572204 28.701495522162496 22.477145049624912 14-15 22.35101853367305 25.270139216294584 26.053909773640182 26.32493247639218 16-17 21.820006084242657 28.769950485729716 26.596445994529393 22.813597435498234 18-19 21.733217380541753 27.487602659748582 28.97759603154828 21.801583928161385 20-21 22.774956420231433 26.751724299662044 28.71646181786914 21.75685746223738 22-23 22.413887446722164 26.528893964157685 28.73915470717791 22.31806388194224 24-25 22.288159083158614 26.63900933915229 28.725050835566364 22.347780742122726 26-27 21.978753033367408 26.73921614577947 28.78214359754891 22.49988722330421 28-29 22.076145270857317 26.758882537378792 28.84617357661421 22.318798615149678 30-31 22.315966556251485 27.132446103386393 28.41617728964931 22.135410050712814 32-33 22.04753582139721 26.808592966264438 28.855995247264705 22.28787596507365 34-35 22.233987529281173 26.628101720000313 28.81733367380706 22.32057707691145 36-37 22.48766542723807 26.886388390193318 28.274377962316855 22.35156822025176 38-39 22.3057410645189 27.10881457818171 28.404188999398087 22.181255357901293 40-41 22.210502144790457 26.870560186044695 28.562224979887503 22.35671268927735 42-43 22.223353153768187 26.847270825136345 28.414758233254034 22.51461778784144 44-45 22.281622365997098 27.034325093347995 28.284996052415583 22.399056488239324 46-47 22.386777285885888 27.21427892094148 28.018924954205417 22.38001883896722 48-49 22.44110542786343 27.10050901690817 27.81348445329213 22.64490110193627 50-51 22.465614976327522 27.140880558863305 27.709192660359218 22.68431180444996 52-53 22.518005154007906 27.124318292558996 27.72238384591132 22.635292707521774 54-55 22.486395164925646 27.496407437613833 27.59742586228011 22.419771535180402 56-57 22.317743414829888 27.258168112377174 27.669472534974553 22.75461593781838 58-59 22.25610312184877 26.875493448052133 28.03625263498003 22.832150795119066 60-61 22.4875677147525 27.165829813512467 27.751078745840708 22.595523725894328 62-63 22.303754243978958 27.231411176744952 27.717823929918 22.747010649358092 64-65 22.336048220460214 26.99537038243364 27.79184113773813 22.876740259368024 66-67 22.193977880821038 27.059865029721703 27.686071880898883 23.060085208558377 68-69 22.512121257523063 27.184529738201686 27.64757382681275 22.655775177462505 70-71 22.533072831804375 26.999527560055327 27.634316825946186 22.83308278219411 72-73 22.40648317728061 27.095816818517743 27.642894343505343 22.854805660696307 74-75 22.431640256033102 27.190521355417253 27.5222393906902 22.855598997859442 76-77 22.438715126204652 27.096670071947344 27.472032646398166 22.992582155449842 78-79 22.50846190125046 27.19146305556062 27.52060756320695 22.779467479981967 80-81 22.455029457057314 27.0514410637372 27.566125296068833 22.927404183136648 82-83 22.34787698246613 27.103922866991354 27.649810486449056 22.89838966409346 84-85 22.31828083350053 27.01882137897071 27.558340868051665 23.104556919477094 86-87 22.42432503604384 27.103420455768717 27.52350442696361 22.948750081223835 88-89 22.17662207460803 27.052841288197655 27.582421859668475 23.188114777525836 90-91 22.118874621661522 26.97320260213816 27.617809126049313 23.29011365015101 92-93 22.24898476066148 27.098519581058245 27.492547745888558 23.15994791239172 94-95 22.070030589058 26.833507289634877 27.678317694637393 23.418144426669723 96-97 22.05843411578362 26.809858033298518 27.670006159153704 23.46170169176416 98-99 21.943542322087374 26.945367918809964 27.588121276947092 23.522968482155566 100 22.13627504371005 26.769247405407796 27.516910774169506 23.57756677671264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 19.0 2 72.5 3 117.0 4 80.0 5 71.0 6 167.0 7 371.5 8 658.5 9 804.0 10 681.0 11 582.5 12 658.5 13 791.0 14 919.5 15 1098.0 16 1317.0 17 1502.0 18 1739.5 19 2068.0 20 2492.5 21 3052.0 22 3897.5 23 5294.0 24 7205.0 25 9378.5 26 12276.0 27 16478.0 28 22090.5 29 28890.5 30 36047.0 31 43538.0 32 51865.5 33 60782.0 34 70103.0 35 78445.0 36 86142.0 37 94423.5 38 100913.5 39 104777.0 40 108311.5 41 111287.5 42 112670.5 43 113788.5 44 113839.0 45 113203.5 46 113130.5 47 110859.0 48 107822.5 49 105922.0 50 104210.5 51 101725.5 52 96152.5 53 91698.0 54 88584.0 55 83957.5 56 77234.0 57 69848.0 58 64037.0 59 58584.5 60 51973.0 61 44224.5 62 37970.5 63 31912.0 64 25391.5 65 20186.5 66 15856.5 67 11780.5 68 8859.5 69 6918.0 70 4964.0 71 3480.5 72 2645.0 73 1923.0 74 1388.0 75 884.5 76 474.5 77 308.0 78 190.5 79 107.0 80 58.0 81 24.5 82 13.0 83 6.0 84 3.5 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04625057650366487 2 0.24177326013148193 3 0.0026056662818966124 4 0.003159370366799642 5 2.605666281896612E-4 6 6.51416570474153E-5 7 0.0 8 1.6285414261853827E-4 9 0.0 10-11 5.048478421174686E-4 12-13 0.0 14-15 3.094228709752227E-4 16-17 1.9542497114224592E-4 18-19 5.537040849030302E-4 20-21 0.0 22-23 1.9542497114224592E-4 24-25 0.0012702623124245984 26-27 1.791395568803921E-4 28-29 2.768520424515151E-4 30-31 6.51416570474153E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.51416570474153E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6285414261853825E-5 68-69 1.9542497114224592E-4 70-71 1.6285414261853825E-5 72-73 2.931374567133689E-4 74-75 1.302833140948306E-4 76-77 1.6285414261853825E-5 78-79 0.0 80-81 0.0 82-83 9.771248557112296E-5 84-85 0.0 86-87 4.071353565463457E-4 88-89 0.002165960096826559 90-91 0.002100818439779144 92-93 6.514165704741531E-4 94-95 5.374186706411763E-4 96-97 1.6285414261853827E-4 98-99 9.771248557112296E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3070232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.77358957438598 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42210280381933 45.08249817832642 2 14.389888292581068 17.202705536440053 3 4.63425765045887 8.310186443414604 4 1.9311604540012042 4.617295695190115 5 1.028521992518816 3.0739225724524695 6 0.6024538167882698 2.160649630933462 7 0.4036505357167666 1.6889349017390525 8 0.2748265112283114 1.3141893669057174 9 0.20745432341337136 1.116026062482851 >10 1.0289645219283148 10.898417932454961 >50 0.05669514487613781 2.290324602817813 >100 0.019696806403976568 1.8973619408554563 >500 1.0904875564974613E-4 0.0345792396608417 >1k 2.1809750996526267E-4 0.31290789632623967 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3793 0.12354115259042313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3172 0.10331466807720069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.257082852370766E-5 0.0 8 0.0 0.0 0.0 3.257082852370766E-5 0.0 9 0.0 0.0 0.0 1.6285414261853827E-4 0.0 10-11 1.628541426185383E-5 0.0 0.0 1.6285414261853827E-4 0.0 12-13 3.257082852370766E-5 0.0 0.0 1.791395568803921E-4 0.0 14-15 3.257082852370766E-5 0.0 0.0 1.9542497114224592E-4 0.0 16-17 3.257082852370766E-5 0.0 0.0 1.9542497114224592E-4 0.0 18-19 3.257082852370766E-5 0.0 0.0 2.4428121392780744E-4 0.0 20-21 3.257082852370766E-5 0.0 0.0 3.094228709752227E-4 0.0 22-23 4.8856242785561486E-5 0.0 0.0 3.2570828523707655E-4 0.0 24-25 6.514165704741531E-5 0.0 0.0 3.582791137607842E-4 0.0 26-27 6.514165704741531E-5 0.0 0.0 4.72277013593761E-4 0.0 28-29 6.514165704741531E-5 0.0 0.0 9.771248557112295E-4 0.0 30-31 6.514165704741531E-5 0.0 0.0 0.0030616578812285196 1.628541426185383E-5 32-33 6.514165704741531E-5 3.257082852370766E-5 0.0 0.008110136302403205 3.257082852370766E-5 34-35 6.514165704741531E-5 6.514165704741531E-5 0.0 0.01578056641973636 3.257082852370766E-5 36-37 6.514165704741531E-5 6.514165704741531E-5 0.0 0.028988037386099813 3.257082852370766E-5 38-39 6.514165704741531E-5 6.514165704741531E-5 0.0 0.055012129376542235 3.257082852370766E-5 40-41 6.514165704741531E-5 6.514165704741531E-5 0.0 0.08719210795796539 3.257082852370766E-5 42-43 6.514165704741531E-5 6.514165704741531E-5 0.0 0.12119605293671618 3.257082852370766E-5 44-45 6.514165704741531E-5 6.514165704741531E-5 0.0 0.1598087701515716 3.257082852370766E-5 46-47 6.514165704741531E-5 6.514165704741531E-5 0.0 0.2077367443242074 3.257082852370766E-5 48-49 9.771248557112296E-5 6.514165704741531E-5 0.0 0.26574538992493074 3.257082852370766E-5 50-51 9.771248557112296E-5 6.514165704741531E-5 0.0 0.32753225163440414 3.257082852370766E-5 52-53 9.771248557112296E-5 9.771248557112296E-5 0.0 0.39203877752560723 3.257082852370766E-5 54-55 9.771248557112296E-5 9.771248557112296E-5 0.0 0.46335260657826505 3.257082852370766E-5 56-57 1.3028331409483063E-4 1.139978998329768E-4 0.0 0.5458870860573404 3.257082852370766E-5 58-59 1.3028331409483063E-4 1.3028331409483063E-4 0.0 0.6425247342871809 3.257082852370766E-5 60-61 1.3028331409483063E-4 1.3028331409483063E-4 0.0 0.7480867895325174 3.257082852370766E-5 62-63 1.3028331409483063E-4 1.3028331409483063E-4 0.0 0.8550168195758496 3.257082852370766E-5 64-65 1.3028331409483063E-4 1.3028331409483063E-4 0.0 0.9670604696974039 3.257082852370766E-5 66-67 1.3028331409483063E-4 1.3028331409483063E-4 0.0 1.0951289674526223 3.257082852370766E-5 68-69 1.3028331409483063E-4 1.3028331409483063E-4 0.0 1.2433913788925397 3.257082852370766E-5 70-71 1.4656872835668445E-4 1.3028331409483063E-4 0.0 1.4131016809153185 3.257082852370766E-5 72-73 1.6285414261853827E-4 1.3028331409483063E-4 0.0 1.5862482053473483 3.257082852370766E-5 74-75 1.6285414261853827E-4 1.3028331409483063E-4 0.0 1.76722801403933 3.257082852370766E-5 76-77 1.6285414261853827E-4 1.3028331409483063E-4 0.0 1.9615618624260316 3.257082852370766E-5 78-79 1.6285414261853827E-4 1.3028331409483063E-4 0.0 2.1917724784315977 3.257082852370766E-5 80-81 1.791395568803921E-4 1.3028331409483063E-4 0.0 2.441265024923198 3.257082852370766E-5 82-83 1.9542497114224592E-4 1.3028331409483063E-4 0.0 2.7041116111095187 3.257082852370766E-5 84-85 1.9542497114224592E-4 1.3028331409483063E-4 0.0 2.9686355949648107 3.257082852370766E-5 86-87 1.9542497114224592E-4 1.3028331409483063E-4 0.0 3.2484841536405065 3.257082852370766E-5 88 1.9542497114224592E-4 1.3028331409483063E-4 0.0 3.4812352942709217 3.257082852370766E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3860 0.0 50.06325 1 GTATCAA 7040 0.0 43.450474 1 TCAACGC 8365 0.0 35.677197 4 ATCAACG 8430 0.0 35.40153 3 CAACGCA 8570 0.0 34.659256 5 AACGCAG 8945 0.0 33.206238 6 TATCAAC 9125 0.0 33.041817 2 ACGCAGA 10470 0.0 28.234941 7 CGCAGAG 10655 0.0 27.568272 8 GTACATG 9170 0.0 25.442415 1 TACATGG 9295 0.0 24.176079 2 GCAGAGT 12100 0.0 23.887611 9 ACATGGG 9590 0.0 22.151123 3 GAGTACT 7140 0.0 21.128838 12-13 AGAGTAC 10920 0.0 20.314156 10-11 GTACCGT 140 5.2144553E-4 20.142166 6 CAGAGTA 11550 0.0 20.019928 10-11 GAGTACA 7050 0.0 19.922768 1 GTGGTAT 2255 0.0 19.229315 1 GTACTTT 7950 0.0 18.8283 14-15 >>END_MODULE