FastQCFastQC Report
Wed 25 May 2016
SRR1033045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770792
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.033.1404271
TCAACGC29300.032.082864
GTATCAA31650.031.632411
ATCAACG30850.030.4709193
CAACGCA31400.030.0879655
AACGCAG32000.029.5216836
GTACATG70400.027.3071921
TACATGG69800.025.588152
TATCAAC38150.025.1331162
ACATGGG68500.025.0445383
ACGCAGA39050.023.832127
CGCAGAG40200.022.7015368
CATGGGG51050.020.1631834
TACACCG3404.9112714E-1119.3542145
TAATGCG1801.3376868E-418.2789785
TATACTG16300.017.8784755
GCAGAGT50550.017.2174729
GTATAGA11900.016.984311
GTATAGG11200.016.7868181
GAGTACA50900.016.0678541
Produced by FastQC (version 0.11.5)