##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033045_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2770792
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179155634923156	33.0	31.0	34.0	28.0	34.0
2	31.187014398771183	33.0	31.0	34.0	28.0	34.0
3	31.375172874759276	33.0	31.0	34.0	28.0	34.0
4	34.799224553845974	37.0	35.0	37.0	32.0	37.0
5	34.81858219599306	37.0	35.0	37.0	32.0	37.0
6	34.888331206384315	37.0	35.0	37.0	32.0	37.0
7	34.74683772726354	37.0	35.0	37.0	32.0	37.0
8	34.724617726628345	37.0	35.0	37.0	32.0	37.0
9	36.323509307086205	39.0	37.0	39.0	32.0	39.0
10-11	36.347323436764654	39.0	37.0	39.0	32.0	39.0
12-13	36.298007212378266	39.0	37.0	39.0	32.0	39.0
14-15	37.60981805924082	40.0	38.0	41.0	32.0	41.0
16-17	37.58555911089681	40.0	38.0	41.0	32.0	41.0
18-19	37.563710303768744	40.0	38.0	41.0	32.0	41.0
20-21	37.591717277947964	40.0	38.0	41.0	32.0	41.0
22-23	37.52248851591891	40.0	38.0	41.0	32.0	41.0
24-25	37.51720663261624	40.0	38.0	41.0	32.0	41.0
26-27	37.42170162899272	40.0	38.0	41.0	31.5	41.0
28-29	37.42980202050533	40.0	38.0	41.0	31.5	41.0
30-31	37.578417650982104	40.0	38.0	41.0	32.0	41.0
32-33	37.574958531712234	40.0	38.0	41.0	31.5	41.0
34-35	37.52597596643848	40.0	38.0	41.0	31.0	41.0
36-37	37.442451472358805	40.0	38.0	41.0	31.0	41.0
38-39	37.342670435023635	40.0	38.0	41.0	31.0	41.0
40-41	37.26767581254746	40.0	38.0	41.0	30.5	41.0
42-43	37.13058630889652	40.0	38.0	41.0	30.0	41.0
44-45	36.973441167723884	40.0	37.0	41.0	30.0	41.0
46-47	36.82941718468943	40.0	37.0	41.0	30.0	41.0
48-49	36.70129388997803	40.0	37.0	41.0	30.0	41.0
50-51	36.56773785257067	40.0	36.5	41.0	30.0	41.0
52-53	36.404542455731075	40.0	36.0	41.0	30.0	41.0
54-55	36.24142573675685	40.0	36.0	41.0	29.0	41.0
56-57	36.06354843669247	39.0	35.0	41.0	29.0	41.0
58-59	35.8613465030937	39.0	35.0	41.0	28.5	41.0
60-61	35.6322448599534	39.0	35.0	41.0	28.0	41.0
62-63	35.377393539464535	38.5	35.0	40.5	28.0	41.0
64-65	35.09240841607742	38.0	34.5	40.0	28.0	41.0
66-67	34.78651248451706	37.5	34.0	40.0	27.5	41.0
68-69	34.46819609700043	37.0	34.0	40.0	27.0	41.0
70-71	34.1293130267447	36.5	34.0	39.0	26.0	41.0
72-73	33.77057083317694	36.0	34.0	39.0	26.0	41.0
74-75	33.40729780510409	35.5	33.5	39.0	26.0	40.0
76-77	33.039990370984185	35.0	33.0	37.5	26.0	40.0
78-79	32.648925830592844	35.0	33.0	37.0	25.0	39.0
80-81	32.293742547257246	35.0	33.0	37.0	25.0	39.0
82-83	32.07268715948364	35.0	33.0	36.0	25.0	38.5
84-85	31.846059177303818	35.0	33.0	36.0	24.5	37.0
86-87	31.666933497714734	35.0	33.0	35.5	24.5	37.0
88-89	31.447347004033503	35.0	33.0	35.0	24.0	36.5
90-91	31.232691050067995	35.0	32.5	35.0	23.5	36.0
92-93	31.004880012646204	35.0	32.0	35.0	21.5	36.0
94-95	30.792602981385826	35.0	32.0	35.0	20.0	36.0
96-97	30.60478592402461	35.0	32.0	35.0	18.5	35.0
98-99	30.409252119971473	35.0	32.0	35.0	9.5	35.0
100	30.257034811707268	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.04457965832240873
1101	2	0.08987298152966972
1101	3	0.11311808855359118
1101	4	-0.03442339040947218
1101	5	0.04201187621945479
1101	6	0.11414698256368894
1101	7	0.14728032366827648
1101	8	0.17638206293526082
1101	9	0.3205602195895878
1101	10-11	0.2986466873742515
1101	12-13	0.28489614623818227
1101	14-15	0.3328939253871326
1101	16-17	0.33447811237302005
1101	18-19	0.25106993879375494
1101	20-21	0.3466188450725909
1101	22-23	0.36846426580017777
1101	24-25	0.3223596548353598
1101	26-27	0.19681439168657278
1101	28-29	0.24970053713526852
1101	30-31	0.2954916007131345
1101	32-33	0.26601186630317386
1101	34-35	0.2892235780263377
1101	36-37	0.34869427263748776
1101	38-39	0.471285358623895
1101	40-41	0.4612529471717153
1101	42-43	0.4415769717913207
1101	44-45	0.43761033425444396
1101	46-47	0.27408526933737676
1101	48-49	0.3464998526488472
1101	50-51	0.29403289544669775
1101	52-53	0.28146087915645523
1101	54-55	0.26436317713505275
1101	56-57	0.3678193128266116
1101	58-59	0.40636713776472533
1101	60-61	0.4605369646013884
1101	62-63	0.4023019368887546
1101	64-65	0.36438938023628964
1101	66-67	0.3697536632776135
1101	68-69	0.4259966792486267
1101	70-71	0.2876484083208908
1101	72-73	0.28439718477199705
1101	74-75	0.2487430663627137
1101	76-77	0.29193851858525477
1101	78-79	0.34406757890231177
1101	80-81	0.35952667283049067
1101	82-83	0.4166845003742239
1101	84-85	0.298531950080136
1101	86-87	0.3727939247493808
1101	88-89	0.4154868072396667
1101	90-91	0.39778653748042103
1101	92-93	0.36060543942650725
1101	94-95	0.40233346644325607
1101	96-97	0.5340556398145964
1101	98-99	0.4911121445225426
1101	100	0.4753833662686944
1102	1	0.13004756612095036
1102	2	0.2238098533294739
1102	3	0.27480598959942526
1102	4	0.15389210154911126
1102	5	0.15134884152463002
1102	6	0.25369438491697593
1102	7	0.3363116771106789
1102	8	0.3718247376052517
1102	9	0.426471083073821
1102	10-11	0.3738219266851459
1102	12-13	0.41024830054620054
1102	14-15	0.5163854185214021
1102	16-17	0.4885371656373536
1102	18-19	0.46588042388514594
1102	20-21	0.4348285294149363
1102	22-23	0.40951170916093815
1102	24-25	0.4077606392645947
1102	26-27	0.2273744330853944
1102	28-29	0.3231949903016087
1102	30-31	0.39599593022840196
1102	32-33	0.3529777959593261
1102	34-35	0.36471719772091404
1102	36-37	0.43996390574755395
1102	38-39	0.4195383984852441
1102	40-41	0.3659937237159383
1102	42-43	0.40809436707966995
1102	44-45	0.3508522521387363
1102	46-47	0.4086247637006153
1102	48-49	0.3132823394171709
1102	50-51	0.3499379512730698
1102	52-53	0.40342148616701934
1102	54-55	0.38929693028502754
1102	56-57	0.3880625392032826
1102	58-59	0.3079119331249274
1102	60-61	0.3225090245630611
1102	62-63	0.3750839007597122
1102	64-65	0.3653878186216204
1102	66-67	0.325004722706808
1102	68-69	0.36961327224754825
1102	70-71	0.29093681244729197
1102	72-73	0.2739806612151767
1102	74-75	0.3166623761381899
1102	76-77	0.3410553799021301
1102	78-79	0.25343241680204187
1102	80-81	0.25736226906879267
1102	82-83	0.2109810056629442
1102	84-85	0.24963292797314907
1102	86-87	0.2863185236150727
1102	88-89	0.23692911721372667
1102	90-91	0.3100790386076042
1102	92-93	0.42868336695443077
1102	94-95	0.4163108752786364
1102	96-97	0.3322427554735832
1102	98-99	0.3818757203911609
1102	100	0.4687474406383565
1103	1	0.1128023198737722
1103	2	0.1806683383248
1103	3	0.16212304856158255
1103	4	0.17690076832698765
1103	5	0.12886407392296917
1103	6	0.22664267817341255
1103	7	0.20816040436800165
1103	8	0.21314763267448456
1103	9	0.3236237301085012
1103	10-11	0.3704627889673162
1103	12-13	0.3604617262404304
1103	14-15	0.3984105264920288
1103	16-17	0.5136994194762394
1103	18-19	0.3845085018093499
1103	20-21	0.37874900848412807
1103	22-23	0.44579434197606815
1103	24-25	0.44691584687801367
1103	26-27	0.360684993950116
1103	28-29	0.3719231082100407
1103	30-31	0.42125092891766514
1103	32-33	0.3641721192595284
1103	34-35	0.38163846588261663
1103	36-37	0.44955866172465875
1103	38-39	0.4627827867929071
1103	40-41	0.5021428992731316
1103	42-43	0.47094452688414634
1103	44-45	0.43444919003610494
1103	46-47	0.3979286543504017
1103	48-49	0.36968961177854
1103	50-51	0.4462225303398739
1103	52-53	0.43585868314093545
1103	54-55	0.46498679044618996
1103	56-57	0.586350168721701
1103	58-59	0.3973472288625075
1103	60-61	0.40305120791320803
1103	62-63	0.34865704672328945
1103	64-65	0.42784071800702606
1103	66-67	0.44063113771121465
1103	68-69	0.37474290320115955
1103	70-71	0.3184419825920557
1103	72-73	0.4197725208520353
1103	74-75	0.4277240365689039
1103	76-77	0.3724202186810004
1103	78-79	0.4345967386988292
1103	80-81	0.41299064356468307
1103	82-83	0.377137079159759
1103	84-85	0.4204044645057472
1103	86-87	0.3587365625877723
1103	88-89	0.3498258149409814
1103	90-91	0.36603198698030326
1103	92-93	0.4782025633510898
1103	94-95	0.4798450697290413
1103	96-97	0.4981254931479846
1103	98-99	0.482360694533817
1103	100	0.3835998565059988
1104	1	0.08153091605921148
1104	2	0.11787949255506547
1104	3	0.20043194515539398
1104	4	0.2470625507784021
1104	5	0.16193549703464072
1104	6	0.1786519583285937
1104	7	0.22537756075102067
1104	8	0.24494306577554426
1104	9	0.28271335766176975
1104	10-11	0.2530539458791239
1104	12-13	0.36865240924531406
1104	14-15	0.46912321634613363
1104	16-17	0.3957746606587733
1104	18-19	0.3992188822756688
1104	20-21	0.4177377835813303
1104	22-23	0.3341213452728624
1104	24-25	0.19960080778883338
1104	26-27	0.26787774074321646
1104	28-29	0.24224261946689296
1104	30-31	0.25491038130579824
1104	32-33	0.2516213233790907
1104	34-35	0.2666488098849129
1104	36-37	0.3249418158496624
1104	38-39	0.2566892233812297
1104	40-41	0.35168866537463117
1104	42-43	0.36043580997900193
1104	44-45	0.3445498482680023
1104	46-47	0.3133059754856262
1104	48-49	0.3658039383783489
1104	50-51	0.2855091575685975
1104	52-53	0.3445393435531656
1104	54-55	0.3095992004922081
1104	56-57	0.3377324494645606
1104	58-59	0.3694466791455042
1104	60-61	0.40428700605541934
1104	62-63	0.4131820371060613
1104	64-65	0.3909812661201144
1104	66-67	0.41299667507932014
1104	68-69	0.3575401569969898
1104	70-71	0.39598860578592365
1104	72-73	0.40654779849858613
1104	74-75	0.46351989195085963
1104	76-77	0.37651089253855474
1104	78-79	0.37942476887749876
1104	80-81	0.4000387748151013
1104	82-83	0.40112185073336803
1104	84-85	0.4139279036014365
1104	86-87	0.3853599318880754
1104	88-89	0.3953046839800223
1104	90-91	0.4333892666893888
1104	92-93	0.3957050726017961
1104	94-95	0.5149249271810454
1104	96-97	0.49327665592411307
1104	98-99	0.584471033899792
1104	100	0.5959359720480677
1105	1	0.15186175802843138
1105	2	0.14763643308603847
1105	3	0.08860764418037093
1105	4	0.1419572529102595
1105	5	0.18313786208818783
1105	6	0.24616582209704774
1105	7	0.19300371814668438
1105	8	0.31138629408432195
1105	9	0.3220032171106908
1105	10-11	0.39817996351573726
1105	12-13	0.4033774766043763
1105	14-15	0.48980707678978774
1105	16-17	0.38600869988894715
1105	18-19	0.4033487249571053
1105	20-21	0.3880831028477658
1105	22-23	0.35267189100572693
1105	24-25	0.2910402216210315
1105	26-27	0.32702878867196006
1105	28-29	0.3687008198589865
1105	30-31	0.37772764052586183
1105	32-33	0.3441548509920196
1105	34-35	0.309706099210068
1105	36-37	0.32267165435184353
1105	38-39	0.3985421769149937
1105	40-41	0.4016022549451108
1105	42-43	0.3688207831089301
1105	44-45	0.4160845696725062
1105	46-47	0.32753762100789174
1105	48-49	0.3187732884810117
1105	50-51	0.2782562778564426
1105	52-53	0.3898793741926738
1105	54-55	0.373764281071189
1105	56-57	0.39058947591659177
1105	58-59	0.24907047477818622
1105	60-61	0.293222958336429
1105	62-63	0.3660314736353669
1105	64-65	0.3447800734814521
1105	66-67	0.31218214546927925
1105	68-69	0.27319771121284475
1105	70-71	0.20566648982595126
1105	72-73	0.28341994691088246
1105	74-75	0.23492234814352742
1105	76-77	0.21038997173921814
1105	78-79	0.3051806432164881
1105	80-81	0.3521211427701161
1105	82-83	0.27657794414130166
1105	84-85	0.28967423349250865
1105	86-87	0.2690399827789882
1105	88-89	0.25590764689610523
1105	90-91	0.25558517904764244
1105	92-93	0.2690455350983001
1105	94-95	0.34248902855190977
1105	96-97	0.335321477179928
1105	98-99	0.32384065358882097
1105	100	0.3273427179881949
1106	1	-0.015492216515259116
1106	2	-0.01041417151210311
1106	3	0.033199250766251964
1106	4	0.00188980506143821
1106	5	-0.004816579667874521
1106	6	0.059644510390398864
1106	7	0.09608579408956075
1106	8	0.1707252015328038
1106	9	0.1341712926591896
1106	10-11	0.17059900378725246
1106	12-13	0.162839102866144
1106	14-15	0.2385411845883283
1106	16-17	0.25119979493054245
1106	18-19	0.26500244948604745
1106	20-21	0.21159810589198713
1106	22-23	0.19450187809476915
1106	24-25	0.21042484276303952
1106	26-27	0.1390831557258565
1106	28-29	0.13817682520760854
1106	30-31	0.23110330028701753
1106	32-33	0.22389953943794438
1106	34-35	0.22486767322349976
1106	36-37	0.2461927890186857
1106	38-39	0.3055073835549891
1106	40-41	0.30268625673566163
1106	42-43	0.3224511498979865
1106	44-45	0.2753971370519892
1106	46-47	0.2546239056044186
1106	48-49	0.24605371234022044
1106	50-51	0.24109067078981639
1106	52-53	0.16389776042444737
1106	54-55	0.1939352180435776
1106	56-57	0.20920136867363937
1106	58-59	0.25818914758415445
1106	60-61	0.2707025159959855
1106	62-63	0.2711526163625493
1106	64-65	0.2845419765298445
1106	66-67	0.2774935412194637
1106	68-69	0.2805999902765208
1106	70-71	0.3972900888005668
1106	72-73	0.31628319877231803
1106	74-75	0.25229691975756907
1106	76-77	0.340031127881133
1106	78-79	0.28350026196197575
1106	80-81	0.31644842748323043
1106	82-83	0.2593467028043719
1106	84-85	0.22039184924127042
1106	86-87	0.2787197422351433
1106	88-89	0.3208721309833926
1106	90-91	0.31287741995419793
1106	92-93	0.33842167848039395
1106	94-95	0.3116909532480996
1106	96-97	0.3013652132092588
1106	98-99	0.25696586679224964
1106	100	0.2020325600773205
1107	1	-0.13169119776354066
1107	2	-0.09039842189237035
1107	3	-0.0923715831484806
1107	4	-0.07766461885477582
1107	5	-0.07396691531437938
1107	6	-0.012586727184327628
1107	7	-0.12165573807212837
1107	8	-0.16538317795566826
1107	9	-0.18186450042396274
1107	10-11	-0.035411189256116415
1107	12-13	-0.040036584280095155
1107	14-15	-0.03670107633866593
1107	16-17	-0.13176174145867492
1107	18-19	-0.0706820994462305
1107	20-21	-0.05745565971775335
1107	22-23	-0.08926327573341553
1107	24-25	-0.061864972344714886
1107	26-27	-0.0314551635134066
1107	28-29	-0.12897007591656262
1107	30-31	-0.048135744401839986
1107	32-33	-0.10805632846972202
1107	34-35	-0.09369839297740157
1107	36-37	0.01710785574042717
1107	38-39	-0.03360364711947028
1107	40-41	-0.03870285972448784
1107	42-43	-0.004957664639185566
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2216	88-89	-1.0612688301991824
2216	90-91	-1.034077138727973
2216	92-93	-1.3090372938209498
2216	94-95	-1.262929911385374
2216	96-97	-1.185917567062173
2216	98-99	-1.1069549286796168
2216	100	-1.0522746459574996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47495.0
3	9087.0
4	4918.0
5	3906.0
6	3346.0
7	3480.0
8	4128.0
9	4732.0
10	5260.0
11	5812.0
12	6407.0
13	6856.0
14	7458.0
15	7761.0
16	7588.0
17	7767.0
18	7680.0
19	7725.0
20	7745.0
21	8014.0
22	9153.0
23	11949.0
24	17200.0
25	21902.0
26	22327.0
27	21639.0
28	24624.0
29	30396.0
30	36799.0
31	44970.0
32	57033.0
33	76530.0
34	108436.0
35	163471.0
36	287854.0
37	567435.0
38	817072.0
39	284472.0
40	365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22294676909754	19.54510638343974	13.350934802698507	23.881012044764212
2	15.45125092025891	19.849718425144992	40.5228767609486	24.1761538936475
3	18.28885204734768	25.053941240430056	29.732845060535162	26.9243616516871
4	12.054406328984998	16.605567478425066	39.22369237503801	32.116333817551926
5	12.86218246497199	38.031055254454266	35.76826824295719	13.338494037616549
6	31.28413642432334	38.12363093070703	17.77062779232339	12.82160485264624
7	27.665946114654467	32.167999398897784	22.50481170762157	17.661242778826175
8	24.94403731905159	37.027821653547285	20.175359367842088	17.85278165955903
9	24.71806969502106	17.169895161232056	20.999111290940224	37.11292385280666
10-11	23.81473058240589	27.082930653578597	28.92727919751871	20.175059566496802
12-13	24.488523632427455	24.285437892561827	28.928011867924543	22.29802660708618
14-15	21.778646358356692	26.351538840076394	25.827734890708427	26.042079910858483
16-17	21.63040359183314	29.71220874008637	26.327438382750472	22.32994928533002
18-19	21.54385053504591	28.769774643262085	28.14125985108201	21.54511497061
20-21	22.722464897919668	28.052295127685305	27.62895327380995	21.596286700585072
22-23	22.27154517961832	27.846044482386105	27.706850823362362	22.175559514633207
24-25	22.130689648139366	27.99109875247886	27.674475984987946	22.20373561439383
26-27	21.92315049141004	27.828007750903687	27.793657319371874	22.455184438314397
28-29	22.019758427848874	27.89011699401758	27.841181388579407	22.248943189554144
30-31	22.24898485793068	28.387244282082712	27.4663202553802	21.89745060460641
32-33	21.82598336426432	28.020746182865963	27.989920537735053	22.16334991513467
34-35	21.94916760899327	27.776473210700015	28.05087751438759	22.223481665919124
36-37	22.32613439089147	28.141488423403622	27.4197645771588	22.112612608546105
38-39	22.182964479307664	28.26432878018564	27.606757573103348	21.94594916740335
40-41	21.911438498596144	27.98366522193474	27.91138648391211	22.193509795557006
42-43	21.862358268126076	27.905088044244618	27.8908931853119	22.34166050231741
44-45	22.04055074875298	27.982140377256	27.80068360516032	22.176625268830698
46-47	21.99325270045494	28.188360908284764	27.73284594809853	22.085540443161765
48-49	21.952514515834544	27.949719977266607	27.816711210607327	22.28105429629152
50-51	22.073650347397418	27.88604237825921	27.73026704291605	22.310040231427323
52-53	22.12693450647567	27.819907481401017	27.724607726016366	22.32855028610695
54-55	22.16016555400459	28.29955017994608	27.561184375660236	21.979099890389094
56-57	21.934753381651454	27.992281861924063	27.706038982556542	22.366925773867944
58-59	21.83974278524717	27.631372471123385	28.069056490997756	22.45982825263169
60-61	22.06623177618561	28.077792741207663	27.68929130042077	22.166684182185957
62-63	22.028549959720753	28.058031010235318	27.645649525217735	22.267769504826195
64-65	21.9607720627803	27.800287768329156	27.79972797803022	22.43921219086032
66-67	21.800926557120683	27.89673700567293	27.64374411421338	22.65859232299301
68-69	22.214009839449822	27.93812891542855	27.688440777303537	22.159420467818094
70-71	22.09486145310691	27.83682164530789	27.755314444632432	22.31300245695277
72-73	21.95075753472049	27.93084719516784	27.8750170632195	22.243378206892167
74-75	21.972040899497145	27.931618456018164	27.74809183048308	22.348248814001614
76-77	21.967680074591385	27.936995266357446	27.635701093502668	22.4596235655485
78-79	22.127036486360836	28.0170691568064	27.685038488655618	22.170855868177146
80-81	22.060542904537105	27.71625586663749	27.88368838270372	22.339512846121682
82-83	21.904647555975234	27.841082870973043	28.02663484159383	22.227634731457893
84-85	21.896154051684668	27.77429949262772	27.825101712760564	22.504444742927046
86-87	22.108434213452803	27.926552485102675	27.694156318691636	22.270856982752893
88-89	21.807955115159302	27.792028853508832	27.82114882907188	22.578867202259982
90-91	21.791097856423328	27.69806511245022	27.920438174945026	22.590398856181423
92-93	22.050034806570785	27.922686807298692	27.735095999699595	22.292182386430927
94-95	21.813056425356073	27.638341909982643	27.88129833109627	22.667303333565012
96-97	21.83050876832862	27.591697037217173	27.938415077008234	22.639379117445973
98-99	21.75062980022957	27.7521737450664	27.868005715051186	22.629190739652845
100	21.893736630233978	27.584580696693905	27.764228464295048	22.757454208777066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	211.0
2	209.5
3	225.0
4	244.5
5	258.5
6	256.5
7	230.0
8	229.5
9	224.5
10	199.0
11	191.0
12	194.0
13	194.0
14	222.5
15	281.5
16	351.0
17	446.0
18	599.0
19	865.0
20	1290.5
21	1903.0
22	2823.5
23	4338.5
24	6492.0
25	9244.0
26	12973.0
27	17668.0
28	23484.5
29	30576.5
30	38642.5
31	47234.0
32	55870.0
33	64595.0
34	72963.0
35	80849.5
36	87241.5
37	93109.5
38	97867.0
39	100491.0
40	102513.5
41	103797.5
42	104599.5
43	104862.5
44	103398.0
45	101875.5
46	100909.0
47	98057.5
48	95016.5
49	92836.0
50	90576.0
51	87339.0
52	81323.5
53	76607.5
54	74466.0
55	70596.5
56	64156.0
57	58291.0
58	52370.0
59	45535.0
60	39527.0
61	33765.5
62	28541.5
63	23435.0
64	18896.0
65	15146.5
66	11668.0
67	8748.5
68	6770.0
69	5311.0
70	4126.0
71	3012.5
72	2193.0
73	1664.5
74	1218.0
75	775.0
76	464.5
77	335.0
78	225.5
79	130.0
80	87.5
81	61.5
82	33.5
83	27.0
84	21.5
85	17.5
86	13.5
87	11.5
88	7.5
89	5.0
90	5.0
91	2.5
92	0.5
93	1.0
94	1.5
95	2.0
96	2.5
97	1.0
98	1.0
99	2.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061534752518413505
2	0.04298409985303841
3	0.056842953206159105
4	0.07470788135666624
5	0.0879171009588594
6	0.08979382068376117
7	0.09181490346442461
8	0.10379703709264354
9	0.0985999670852233
10-11	0.10383312785658397
12-13	0.10810988338352356
14-15	0.1103655561297997
16-17	0.07458156368287479
18-19	0.09955637232964437
20-21	0.09311417096627968
22-23	0.058773809076971495
24-25	0.02232213749714883
26-27	0.1869140664474273
28-29	0.06665964099795293
30-31	0.07990495136408651
32-33	0.07194693791522425
34-35	0.021997320621685062
36-37	0.05483991580746588
38-39	0.07920118146724836
40-41	0.0845606599124005
42-43	0.07882222844587397
44-45	0.075375560489564
46-47	0.08217866949233288
48-49	0.07858763848026125
50-51	0.07438306448120248
52-53	0.07793800472933371
54-55	0.06882508683437805
56-57	0.07180257485946256
58-59	0.0685544061048249
60-61	0.0656490996076212
62-63	0.07205521020704549
64-65	0.06850026995891428
66-67	0.06999803666244164
68-69	0.06994390051653102
70-71	0.06176934248402623
72-73	0.06056030189202221
74-75	0.06176934248402623
76-77	0.056716635532367644
78-79	0.05303537761044496
80-81	0.047567626873471554
82-83	0.053666965979402285
84-85	0.04431945811883389
86-87	0.04215401228240878
88-89	0.04386832356957866
90-91	0.040493837141149534
92-93	0.04330891672850218
94-95	0.041847240788915226
96-97	0.04513150030749331
98-99	0.045582634856748544
100	0.053558693687581024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2770792.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.62393026752866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37253673948848	53.67482889628026
2	12.796840786533965	17.30745337986479
3	3.8072008701460374	7.723736584716903
4	1.5523834051757135	4.199130685602846
5	0.7590610969725265	2.566534734523198
6	0.4426885645359288	1.7961804371046008
7	0.2828875634211865	1.3390978203641788
8	0.186271025646189	1.0077103079327143
9	0.13484674152161624	0.8206979980915114
>10	0.6261442833847727	7.2472598561181405
>50	0.03079916677771723	1.40334349508946
>100	0.008232872898559644	0.8758625864522761
>500	1.0688349733119732E-4	0.038163217859036
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0827229182125544E-4	0.0
12-13	0.0	0.0	0.0	1.804538197020924E-4	0.0
14-15	0.0	0.0	0.0	1.9849920167230165E-4	0.0
16-17	0.0	0.0	0.0	2.1654458364251089E-4	0.0
18-19	0.0	0.0	0.0	2.1654458364251089E-4	0.0
20-21	0.0	0.0	0.0	2.1654458364251089E-4	0.0
22-23	0.0	0.0	0.0	2.3458996561272013E-4	0.0
24-25	0.0	0.0	0.0	2.8872611152334785E-4	0.0
26-27	0.0	0.0	0.0	2.8872611152334785E-4	0.0
28-29	0.0	0.0	0.0	5.774522230466957E-4	0.0
30-31	0.0	0.0	0.0	0.0025263534758292938	0.0
32-33	0.0	0.0	0.0	0.006532428273215745	0.0
34-35	0.0	0.0	0.0	0.012794175816878351	0.0
36-37	0.0	0.0	0.0	0.02394622187446766	0.0
38-39	0.0	0.0	0.0	0.044355548882774314	0.0
40-41	0.0	0.0	0.0	0.0714777579839988	0.0
42-43	0.0	0.0	0.0	0.10060300448391651	0.0
44-45	0.0	0.0	0.0	0.13285010206468043	0.0
46-47	0.0	3.609076394041848E-5	0.0	0.17170180944654093	0.0
48-49	0.0	3.609076394041848E-5	0.0	0.2164182659687194	0.0
50-51	0.0	3.609076394041848E-5	0.0	0.26692729010333505	0.0
52-53	0.0	3.609076394041848E-5	0.0	0.31925889781694183	0.0
54-55	0.0	3.609076394041848E-5	0.0	0.37355745216530145	0.0
56-57	0.0	3.609076394041848E-5	0.0	0.44086672691418194	0.0
58-59	0.0	3.609076394041848E-5	0.0	0.519869409179758	0.0
60-61	0.0	3.609076394041848E-5	0.0	0.6067218326023751	0.0
62-63	0.0	3.609076394041848E-5	0.0	0.6964795625221958	0.0
64-65	0.0	3.609076394041848E-5	0.0	0.7924449038397685	0.0
66-67	0.0	7.218152788083696E-5	0.0	0.898046479129433	0.0
68-69	0.0	1.0827229182125544E-4	0.0	1.0207550765268558	0.0
70-71	0.0	1.0827229182125544E-4	0.0	1.157899979500446	0.0
72-73	0.0	1.0827229182125544E-4	0.0	1.3065397907890595	0.0
74-75	0.0	1.4436305576167392E-4	0.0	1.454638240618567	0.0
76-77	0.0	1.4436305576167392E-4	0.0	1.615585002410863	0.0
78-79	0.0	1.4436305576167392E-4	0.0	1.800586258369448	0.0
80-81	0.0	1.4436305576167392E-4	0.0	2.014243580896726	0.0
82-83	0.0	1.4436305576167392E-4	0.0	2.234469422461159	0.0
84-85	0.0	1.4436305576167392E-4	0.0	2.4559764861454774	0.0
86-87	0.0	1.4436305576167392E-4	0.0	2.686740830780513	3.609076394041848E-5
88	0.0	1.4436305576167392E-4	0.0	2.8789602395271823	3.609076394041848E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2170	0.0	33.140427	1
TCAACGC	2930	0.0	32.08286	4
GTATCAA	3165	0.0	31.63241	1
ATCAACG	3085	0.0	30.470919	3
CAACGCA	3140	0.0	30.087965	5
AACGCAG	3200	0.0	29.521683	6
GTACATG	7040	0.0	27.307192	1
TACATGG	6980	0.0	25.58815	2
TATCAAC	3815	0.0	25.133116	2
ACATGGG	6850	0.0	25.044538	3
ACGCAGA	3905	0.0	23.83212	7
CGCAGAG	4020	0.0	22.701536	8
CATGGGG	5105	0.0	20.163183	4
TACACCG	340	4.9112714E-11	19.354214	5
TAATGCG	180	1.3376868E-4	18.278978	5
TATACTG	1630	0.0	17.878475	5
GCAGAGT	5055	0.0	17.217472	9
GTATAGA	1190	0.0	16.98431	1
GTATAGG	1120	0.0	16.786818	1
GAGTACA	5090	0.0	16.067854	1
>>END_MODULE