FastQCFastQC Report
Wed 25 May 2016
SRR1033045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770792
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.12483795246990752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.10899410710006383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35000.050.214371
GTATCAA60050.042.5278551
TCAACGC69250.036.2431344
ATCAACG70000.035.7205283
CAACGCA71050.035.3898165
AACGCAG72950.034.596936
TATCAAC75550.033.3553242
ACGCAGA84850.029.5232357
CGCAGAG87200.028.6203218
GTACATG70800.026.4207121
TACATGG71800.025.0602132
GCAGAGT98650.025.0121389
ACATGGG68900.024.8305553
GTGGTAT17100.022.8701611
TACACCG3900.021.6918475
GAGTACT66350.021.25010512-13
GACGCGT805.4185366E-520.56206110-11
TGGTATC17100.020.3836252
AGAGTAC91800.020.17168210-11
CAGAGTA102250.019.99468610-11
Produced by FastQC (version 0.11.5)