##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033045_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770792 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65824681174191 34.0 31.0 34.0 31.0 34.0 2 32.65185874652446 34.0 31.0 34.0 31.0 34.0 3 32.762696730754236 34.0 31.0 34.0 31.0 34.0 4 36.27040066522496 37.0 37.0 37.0 35.0 37.0 5 36.24986935143453 37.0 37.0 37.0 35.0 37.0 6 36.318669174734154 37.0 37.0 37.0 35.0 37.0 7 36.26326588210158 37.0 37.0 37.0 35.0 37.0 8 36.26610802976189 37.0 37.0 37.0 35.0 37.0 9 38.054402495748505 39.0 39.0 39.0 35.0 39.0 10-11 38.01537376316952 39.0 38.5 39.0 35.0 39.0 12-13 38.000914359504435 39.0 38.0 39.0 35.0 39.0 14-15 39.484622086392626 41.0 39.0 41.0 36.0 41.0 16-17 39.479838616539965 41.0 39.0 41.0 36.0 41.0 18-19 39.48561133423223 41.0 39.0 41.0 36.5 41.0 20-21 39.47693312958894 41.0 39.0 41.0 36.0 41.0 22-23 39.41469496808133 41.0 39.0 41.0 36.0 41.0 24-25 39.36714123615197 41.0 39.0 41.0 36.0 41.0 26-27 39.26158459386342 41.0 39.0 41.0 36.0 41.0 28-29 39.20825579834214 41.0 39.0 41.0 36.0 41.0 30-31 39.21656407265503 41.0 39.0 41.0 36.0 41.0 32-33 39.19243866013761 41.0 39.0 41.0 35.5 41.0 34-35 39.12424642484892 41.0 39.0 41.0 35.0 41.0 36-37 39.04918521491328 41.0 39.0 41.0 35.0 41.0 38-39 38.961715098065824 41.0 39.0 41.0 35.0 41.0 40-41 38.86604840781986 41.0 39.0 41.0 35.0 41.0 42-43 38.76262635376455 41.0 39.0 41.0 35.0 41.0 44-45 38.61263584563547 41.0 38.0 41.0 35.0 41.0 46-47 38.44234590687428 40.5 38.0 41.0 34.5 41.0 48-49 38.26043113304788 40.0 38.0 41.0 34.0 41.0 50-51 38.12838946409546 40.0 38.0 41.0 34.0 41.0 52-53 37.992888134511716 40.0 38.0 41.0 34.0 41.0 54-55 37.84292270946358 40.0 37.5 41.0 33.0 41.0 56-57 37.699388297641974 40.0 37.0 41.0 33.0 41.0 58-59 37.52175262524217 40.0 37.0 41.0 33.0 41.0 60-61 37.32954891597782 40.0 36.0 41.0 33.0 41.0 62-63 37.09650435687702 39.0 36.0 41.0 32.5 41.0 64-65 36.82201713445109 39.0 35.0 41.0 32.0 41.0 66-67 36.53911408723571 39.0 35.0 41.0 32.0 41.0 68-69 36.228058800516244 38.0 35.0 40.0 31.5 41.0 70-71 35.89649547854909 37.0 35.0 40.0 31.0 41.0 72-73 35.54442971540267 37.0 35.0 39.0 31.0 41.0 74-75 35.19017973922257 36.0 35.0 39.0 31.0 41.0 76-77 34.830394703030755 36.0 35.0 39.0 31.0 40.5 78-79 34.464957853205874 35.0 34.5 37.0 30.0 39.0 80-81 34.130079955478436 35.0 34.0 37.0 30.0 39.0 82-83 33.83039722938423 35.0 34.0 36.5 30.0 39.0 84-85 33.60558244718477 35.0 34.0 36.0 30.0 37.0 86-87 33.39584476207525 35.0 34.0 36.0 30.0 37.0 88-89 33.1907981544627 35.0 34.0 35.5 30.0 37.0 90-91 33.008102376504624 35.0 34.0 35.0 30.0 36.0 92-93 32.83348335060877 35.0 34.0 35.0 29.0 36.0 94-95 32.67433589385273 35.0 34.0 35.0 29.0 36.0 96-97 32.529146179143 35.0 34.0 35.0 29.0 36.0 98-99 32.40167017228287 35.0 34.0 35.0 29.0 35.5 100 32.29688406780444 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10408295112259225 1101 2 0.0962706220653331 1101 3 0.14811618280669592 1101 4 0.1282762944504512 1101 5 0.14434419611849592 1101 6 0.11701573293751721 1101 7 0.17960432440712992 1101 8 0.14604666711049674 1101 9 0.24017882875104846 1101 10-11 0.21756461495982649 1101 12-13 0.20857605948955182 1101 14-15 0.31157763060732435 1101 16-17 0.3194238378931118 1101 18-19 0.32114309904560656 1101 20-21 0.30278852238801335 1101 22-23 0.30932426793506806 1101 24-25 0.348874336958616 1101 26-27 0.3730434355793335 1101 28-29 0.3586758591886081 1101 30-31 0.33765775710148915 1101 32-33 0.3029502615592321 1101 34-35 0.28188468081182805 1101 36-37 0.2696839197403378 1101 38-39 0.233151256624204 1101 40-41 0.23925738072858138 1101 42-43 0.1731644387910407 1101 44-45 0.18731554704027076 1101 46-47 0.24360518583132773 1101 48-49 0.2584595426495042 1101 50-51 0.23157402323324305 1101 52-53 0.2321731718510307 1101 54-55 0.3199363707603595 1101 56-57 0.2626370302498344 1101 58-59 0.20910957644914419 1101 60-61 0.16737415137348677 1101 62-63 0.1961156991570121 1101 64-65 0.2146888772580411 1101 66-67 0.217714008736003 1101 68-69 0.20299046042772062 1101 70-71 0.1615750704030816 1101 72-73 0.1289143313584873 1101 74-75 0.15061167705891876 1101 76-77 0.09774447783558315 1101 78-79 0.14237920446334584 1101 80-81 0.1575678307215398 1101 82-83 0.11980235420292473 1101 84-85 0.21404896731500855 1101 86-87 0.2110476774543173 1101 88-89 0.19267153172521034 1101 90-91 0.21478739587184492 1101 92-93 0.2271985740238449 1101 94-95 0.205947468791436 1101 96-97 0.21714534803528807 1101 98-99 0.27339947724696856 1101 100 0.2636568946511062 1102 1 0.17211102438317738 1102 2 0.10399043567073818 1102 3 0.1498757574280205 1102 4 0.13912474955101573 1102 5 0.14971013893034524 1102 6 0.17257628432779626 1102 7 0.17728334285882852 1102 8 0.15604562214839746 1102 9 0.23519589993461665 1102 10-11 0.24295969181450516 1102 12-13 0.24185997569108508 1102 14-15 0.32846781924587987 1102 16-17 0.29565698683851593 1102 18-19 0.28983347427858774 1102 20-21 0.33255995045986 1102 22-23 0.2815577307854227 1102 24-25 0.3054479395507883 1102 26-27 0.3210503265006892 1102 28-29 0.3175896981087405 1102 30-31 0.30642473513356094 1102 32-33 0.34318026146347336 1102 34-35 0.35729555699381166 1102 36-37 0.3167756986363486 1102 38-39 0.3358918466572618 1102 40-41 0.35397069683460813 1102 42-43 0.3412241107062428 1102 44-45 0.3570955554582582 1102 46-47 0.39961833903151955 1102 48-49 0.4373920243595464 1102 50-51 0.4736285644261926 1102 52-53 0.4360266465165239 1102 54-55 0.4725347668476161 1102 56-57 0.4264567996837343 1102 58-59 0.503966968265388 1102 60-61 0.5273694219832876 1102 62-63 0.4246380852842435 1102 64-65 0.4784526100791382 1102 66-67 0.43343102966979785 1102 68-69 0.43679573361453095 1102 70-71 0.43614770796651214 1102 72-73 0.3723868569735913 1102 74-75 0.37403487713251593 1102 76-77 0.36476840913286424 1102 78-79 0.35549672606304483 1102 80-81 0.31989030685706865 1102 82-83 0.3384186245541869 1102 84-85 0.36026726614169746 1102 86-87 0.3350049530757673 1102 88-89 0.41047847685138095 1102 90-91 0.37217971169519615 1102 92-93 0.36962794853285175 1102 94-95 0.4230867414228783 1102 96-97 0.3986475622308632 1102 98-99 0.45095987376636515 1102 100 0.4257973350589097 1103 1 0.14281172794653685 1103 2 0.0984201839290435 1103 3 0.09579829396327 1103 4 0.1121894207139178 1103 5 0.10717911144185166 1103 6 0.09782949564306875 1103 7 0.1555411960505495 1103 8 0.13984976303053998 1103 9 0.1648592910729505 1103 10-11 0.23026178979437617 1103 12-13 0.2093984174716752 1103 14-15 0.22114620578999933 1103 16-17 0.26606985699042696 1103 18-19 0.2713395818017119 1103 20-21 0.2921404736440891 1103 22-23 0.25822376194133767 1103 24-25 0.18810471872933476 1103 26-27 0.19612016648438413 1103 28-29 0.23031407974354323 1103 30-31 0.24523041183335437 1103 32-33 0.1977904285763259 1103 34-35 0.20441828206391932 1103 36-37 0.1797821543664071 1103 38-39 0.22144311251524584 1103 40-41 0.25766605945969445 1103 42-43 0.21777588232486522 1103 44-45 0.19623269073466076 1103 46-47 0.17542735745868043 1103 48-49 0.18506499582246505 1103 50-51 0.15442078153785133 1103 52-53 0.16022709455160822 1103 54-55 0.13708617569551507 1103 56-57 0.10807657095282508 1103 58-59 0.11114499601072225 1103 60-61 0.03923255415098481 1103 62-63 0.011564172735823774 1103 64-65 0.05587973155967063 1103 66-67 0.07638639298335193 1103 68-69 -0.0030650233317288667 1103 70-71 0.07095165289950245 1103 72-73 0.035043715590980185 1103 74-75 0.015609725735316715 1103 76-77 0.007466390591837069 1103 78-79 -5.816750213938349E-5 1103 80-81 -0.026740691768644353 1103 82-83 0.007682676623694817 1103 84-85 0.06575039995113485 1103 86-87 0.08406340417718638 1103 88-89 0.029542938825329657 1103 90-91 -0.027312343096419056 1103 92-93 0.009101904507843983 1103 94-95 0.10065023291894448 1103 96-97 0.08370266630899437 1103 98-99 0.04274887625280144 1103 100 0.004509710836423153 1104 1 0.10247456735324079 1104 2 0.07664928572379637 1104 3 0.10105913699632652 1104 4 0.07807025229401177 1104 5 0.11213922030762546 1104 6 0.12160585217344533 1104 7 0.1519620835421378 1104 8 0.15683942654659688 1104 9 0.20340921357124842 1104 10-11 0.2022465851760984 1104 12-13 0.2345557390329418 1104 14-15 0.2418567027907983 1104 16-17 0.2287263797265524 1104 18-19 0.26929252191676767 1104 20-21 0.2966071591901738 1104 22-23 0.27762537613197935 1104 24-25 0.29800601526951453 1104 26-27 0.2908160302618299 1104 28-29 0.31179819032850986 1104 30-31 0.24954867253729418 1104 32-33 0.2268081117453562 1104 34-35 0.2108769868770466 1104 36-37 0.19991370676871156 1104 38-39 0.21310506976838894 1104 40-41 0.27793184581062036 1104 42-43 0.25979821935514025 1104 44-45 0.23391136128915946 1104 46-47 0.15946495173571407 1104 48-49 0.18697735186164977 1104 50-51 0.32059677117254637 1104 52-53 0.2904951426740894 1104 54-55 0.22926304623071303 1104 56-57 0.31907188200592884 1104 58-59 0.26214703599082156 1104 60-61 0.321279476199436 1104 62-63 0.3007950701641633 1104 64-65 0.27246023997583535 1104 66-67 0.2761974943345251 1104 68-69 0.3190525023277857 1104 70-71 0.3329788580641164 1104 72-73 0.31859361929339514 1104 74-75 0.3439681432220141 1104 76-77 0.3817789310813069 1104 78-79 0.37216784174292883 1104 80-81 0.33488061624955634 1104 82-83 0.3365308522324 1104 84-85 0.32602454420579363 1104 86-87 0.3397801414119641 1104 88-89 0.38126191652965247 1104 90-91 0.3787324449925009 1104 92-93 0.4218243381489657 1104 94-95 0.4415057527066324 1104 96-97 0.4412177938641406 1104 98-99 0.46265920108329794 1104 100 0.4999730732699632 1105 1 0.08363540640238654 1105 2 0.044845708120050176 1105 3 0.08535015865216167 1105 4 0.03883083220494399 1105 5 0.05268670713349621 1105 6 0.09955280329728566 1105 7 0.07230740581091766 1105 8 0.12546536411071685 1105 9 0.1250152796488493 1105 10-11 0.13219327977977002 1105 12-13 0.12848170522139668 1105 14-15 0.19130667658334488 1105 16-17 0.2163557063953334 1105 18-19 0.19898801049432535 1105 20-21 0.20915539708212805 1105 22-23 0.23056539334501025 1105 24-25 0.17945934856361134 1105 26-27 0.15032962309886244 1105 28-29 0.15383010151007426 1105 30-31 0.16467604302957994 1105 32-33 0.09495746341043798 1105 34-35 0.14864412408488903 1105 36-37 0.15727836670744466 1105 38-39 0.10467128454843078 1105 40-41 0.12449672424786229 1105 42-43 0.16514353651993474 1105 44-45 0.20200999737883762 1105 46-47 0.23695752228821476 1105 48-49 0.15302226534943486 1105 50-51 0.15574352959400528 1105 52-53 0.24519231106246764 1105 54-55 0.21045745147289807 1105 56-57 0.18328543240785677 1105 58-59 0.18671632438933727 1105 60-61 0.22985237197292463 1105 62-63 0.2606450094657973 1105 64-65 0.23199559379384027 1105 66-67 0.25020339009522274 1105 68-69 0.193672743486772 1105 70-71 0.1832306135189654 1105 72-73 0.28183812751294113 1105 74-75 0.26658605888631826 1105 76-77 0.1950539821346453 1105 78-79 0.17603228932595272 1105 80-81 0.17062294942181921 1105 82-83 0.15925917004792467 1105 84-85 0.11244201721668645 1105 86-87 0.14977231070135133 1105 88-89 0.1417197221737041 1105 90-91 0.2481357551715675 1105 92-93 0.24244750322961295 1105 94-95 0.17280618568650397 1105 96-97 0.16733291053427735 1105 98-99 0.18838473374545117 1105 100 0.20183106113221072 1106 1 0.02325596547395037 1106 2 0.04499033381039652 1106 3 0.06922934553567472 1106 4 0.07740553220882873 1106 5 0.0316689441394189 1106 6 0.07585100166535597 1106 7 0.10963424864419125 1106 8 0.09758772596569543 1106 9 0.08975131041635365 1106 10-11 0.0911061114235423 1106 12-13 0.08705182809853795 1106 14-15 0.15713425132508974 1106 16-17 0.2000442660253583 1106 18-19 0.13925385807601032 1106 20-21 0.13026605559824844 1106 22-23 0.14599356340110603 1106 24-25 0.13684589555329296 1106 26-27 0.16935688291125928 1106 28-29 0.14943611740911678 1106 30-31 0.15585802124073211 1106 32-33 0.130474326092326 1106 34-35 0.14888078168846164 1106 36-37 0.10494465098732064 1106 38-39 0.10394844396630276 1106 40-41 0.03417133083806334 1106 42-43 0.042718062362709475 1106 44-45 0.05893506060220943 1106 46-47 0.1528042835392398 1106 48-49 0.08024145934332694 1106 50-51 0.04855798755713181 1106 52-53 0.0700464259425928 1106 54-55 0.07508590536842519 1106 56-57 0.08215295337555517 1106 58-59 0.017525166016433502 1106 60-61 0.011436921435397096 1106 62-63 0.0071347130896484146 1106 64-65 0.059474331025015204 1106 66-67 0.004694746090606827 1106 68-69 0.006634763410232836 1106 70-71 -0.003928502088022867 1106 72-73 0.01846455480642817 1106 74-75 0.10147312044291823 1106 76-77 0.006153802360074678 1106 78-79 0.04310075479383357 1106 80-81 0.02188334331380304 1106 82-83 0.13211303736019175 1106 84-85 0.08166725935100061 1106 86-87 -0.014268227096650321 1106 88-89 0.020493138567019287 1106 90-91 0.033818675436002366 1106 92-93 0.0466497373856285 1106 94-95 0.06882897920194608 1106 96-97 0.017234790178875414 1106 98-99 0.04413310116494529 1106 100 0.02162532072438239 1107 1 -0.027247151983679885 1107 2 -0.05246864464716339 1107 3 -0.03910145726665348 1107 4 0.002559802497174246 1107 5 0.042176758852050966 1107 6 -0.0026261482613563203 1107 7 0.010835115702199971 1107 8 -0.029350451250685694 1107 9 0.016171408036363744 1107 10-11 0.013222224804231075 1107 12-13 0.018405369759733503 1107 14-15 0.007378577662741748 1107 16-17 -0.007691062996627807 1107 18-19 -0.06047270642076086 1107 20-21 0.018362575899367073 1107 22-23 0.059859197040083245 1107 24-25 0.05778451762735415 1107 26-27 0.07121453674290734 1107 28-29 0.08443271189923252 1107 30-31 0.03304372998052685 1107 32-33 0.0063597517534219605 1107 34-35 0.037849057257069774 1107 36-37 0.08165726325516687 1107 38-39 0.044278937302408394 1107 40-41 0.007245200699230736 1107 42-43 0.015759410640683313 1107 44-45 0.02653339556346168 1107 46-47 -0.0946455076401449 1107 48-49 -0.13233439096344313 1107 50-51 -0.0971365499497594 1107 52-53 -0.0852993403800184 1107 54-55 -0.10929199155998504 1107 56-57 -0.08324969117901304 1107 58-59 -0.051426752052393 1107 60-61 0.02706676116229545 1107 62-63 0.0035843896007463627 1107 64-65 -0.0735559372573249 1107 66-67 -0.056582990164045555 1107 68-69 -0.05645218968768262 1107 70-71 -0.0167942282905571 1107 72-73 -0.04070914277600224 1107 74-75 -0.033700615822795044 1107 76-77 -0.02277120320064796 1107 78-79 -0.020643565326857072 1107 80-81 -0.047962711061067864 1107 82-83 -0.029229990739068512 1107 84-85 -0.0654174445906861 1107 86-87 -0.00284710022517487 1107 88-89 -0.07013448377920639 1107 90-91 -0.0814234197055157 1107 92-93 -0.003924805979018231 1107 94-95 -0.009209281321624019 1107 96-97 0.023690144842348104 1107 98-99 0.04915689828774106 1107 100 0.04140999302867954 1108 1 -0.04376956284388456 1108 2 -0.04029250642629023 1108 3 0.02084658982984422 1108 4 -0.04629353795178304 1108 5 -0.008777378768442645 1108 6 0.00869969143712268 1108 7 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2214 80-81 -0.20854459520739965 2214 82-83 -0.23196902097898686 2214 84-85 -0.16150267344189473 2214 86-87 -0.1734883504921072 2214 88-89 -0.20017031951304887 2214 90-91 -0.18601796813060645 2214 92-93 -0.22790450284456654 2214 94-95 -0.24095532534833097 2214 96-97 -0.17651811336745027 2214 98-99 -0.23119709415305323 2214 100 -0.24396693055101082 2215 1 -0.24346108659537435 2215 2 -0.17589730025119366 2215 3 -0.216665217720724 2215 4 -0.11891383335817807 2215 5 -0.19878532978553665 2215 6 -0.20938335896768479 2215 7 -0.21834549142386095 2215 8 -0.17985733026716133 2215 9 -0.306963686332395 2215 10-11 -0.2391121011206394 2215 12-13 -0.23213580343897888 2215 14-15 -0.33670423413200723 2215 16-17 -0.3281234096714982 2215 18-19 -0.351897301737786 2215 20-21 -0.3388873806934214 2215 22-23 -0.41669465167917963 2215 24-25 -0.44359173147694264 2215 26-27 -0.4232761510183636 2215 28-29 -0.40188838645933345 2215 30-31 -0.3969430441605297 2215 32-33 -0.39210701231801437 2215 34-35 -0.32047878763173543 2215 36-37 -0.32420717470226634 2215 38-39 -0.3141769594354429 2215 40-41 -0.306518679943359 2215 42-43 -0.276539209686014 2215 44-45 -0.26976442628881614 2215 46-47 -0.2996902145719105 2215 48-49 -0.26177991839454506 2215 50-51 -0.25653973632557836 2215 52-53 -0.20617094283841197 2215 54-55 -0.24812251014768094 2215 56-57 -0.21200056104234477 2215 58-59 -0.23667959920225456 2215 60-61 -0.18801415171839153 2215 62-63 -0.17480793745657763 2215 64-65 -0.18367538165077946 2215 66-67 -0.1861542455402656 2215 68-69 -0.17585695821522762 2215 70-71 -0.2269720832978308 2215 72-73 -0.18607424207380774 2215 74-75 -0.17722041683590817 2215 76-77 -0.196838792476143 2215 78-79 -0.17429826706588614 2215 80-81 -0.12419437184279758 2215 82-83 -0.21987820769532362 2215 84-85 -0.23663011789167143 2215 86-87 -0.17662771107897157 2215 88-89 -0.10825985877549016 2215 90-91 -0.11113135383128281 2215 92-93 -0.10673765929244894 2215 94-95 -0.07018785639764502 2215 96-97 -0.09324066196160175 2215 98-99 -0.1462476860348545 2215 100 -0.16725253433825316 2216 1 -0.35890245618006134 2216 2 -0.2914046205416838 2216 3 -0.27878057644650767 2216 4 -0.24244767889280894 2216 5 -0.28668412775716234 2216 6 -0.30590963366400814 2216 7 -0.2998419246150874 2216 8 -0.2740110661627355 2216 9 -0.40128612629573723 2216 10-11 -0.4059323356275115 2216 12-13 -0.414849725581071 2216 14-15 -0.5433182595940025 2216 16-17 -0.5167880315635571 2216 18-19 -0.4582751198817263 2216 20-21 -0.5225475116667653 2216 22-23 -0.5376165465393967 2216 24-25 -0.509562979304512 2216 26-27 -0.5606586501055375 2216 28-29 -0.6232482977973675 2216 30-31 -0.5236638440314465 2216 32-33 -0.5064988142603966 2216 34-35 -0.5753418305038807 2216 36-37 -0.5250878133957144 2216 38-39 -0.5383009837812409 2216 40-41 -0.5530935354431961 2216 42-43 -0.5409008408022231 2216 44-45 -0.6337830661638435 2216 46-47 -0.5135622125119994 2216 48-49 -0.5278254548269885 2216 50-51 -0.5073492325871598 2216 52-53 -0.5421391690424997 2216 54-55 -0.4801618733345876 2216 56-57 -0.4233164826109075 2216 58-59 -0.4413757565162655 2216 60-61 -0.4327390791088277 2216 62-63 -0.5321612746552731 2216 64-65 -0.5609980087411373 2216 66-67 -0.5770801246880382 2216 68-69 -0.5723667646959711 2216 70-71 -0.5707835134071004 2216 72-73 -0.5610841184622899 2216 74-75 -0.6016498599814497 2216 76-77 -0.5098986193670001 2216 78-79 -0.5984458952774077 2216 80-81 -0.5385885177381269 2216 82-83 -0.5185414279727141 2216 84-85 -0.6353564735365325 2216 86-87 -0.6346323774481917 2216 88-89 -0.694341812106515 2216 90-91 -0.7396708363132873 2216 92-93 -0.7321609601135961 2216 94-95 -0.6628092438612398 2216 96-97 -0.8855571971528349 2216 98-99 -0.8940519924299828 2216 100 -0.8646622630663501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 2.0 4 9.0 5 21.0 6 47.0 7 135.0 8 307.0 9 584.0 10 1036.0 11 1715.0 12 2535.0 13 4015.0 14 4823.0 15 6199.0 16 7879.0 17 10953.0 18 12616.0 19 6757.0 20 4973.0 21 4821.0 22 5107.0 23 5502.0 24 6482.0 25 8173.0 26 10473.0 27 13544.0 28 17863.0 29 23139.0 30 30284.0 31 38457.0 32 49126.0 33 64066.0 34 88082.0 35 132795.0 36 238712.0 37 537434.0 38 979519.0 39 451041.0 40 1553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.816115921056 19.800628897436425 13.567149731243653 23.81610545026392 2 15.76715357832312 20.0844184410916 40.0495932112147 24.098834769370583 3 18.339538064809517 25.17335652814145 29.999043575080258 26.488061831968775 4 12.269839453804396 16.823153762060457 38.865583750702434 32.04142303343272 5 12.651915157770887 37.676402758779986 35.92210163888277 13.74958044456635 6 31.066891055219294 38.272816876348216 17.46376212696095 13.196529941471544 7 27.2526772128691 32.365547468016366 22.525436770425205 17.856338548689326 8 25.219450682153994 36.31213137662942 20.169757129936603 18.298660811279994 9 24.985599785187773 16.346084440838577 21.192857493453136 37.47545828052051 10-11 23.961525510408475 26.606204554616745 28.906863829598333 20.525406105376444 12-13 24.89580596450401 23.8253539060312 29.165325293273547 22.11351483619124 14-15 21.95149855627479 25.734219219942663 26.525944737291816 25.788337486490736 16-17 21.2871304102095 29.27726608082925 26.973249340486284 22.462354168474967 18-19 21.110031390106208 27.927991330953134 29.560800172514757 21.4011771064259 20-21 22.230857944691234 27.241034643362603 29.232199258659215 21.29590815328694 22-23 21.769415304149607 27.066332321708853 29.281403757988244 21.882848616153293 24-25 21.658225513588196 27.218342608195396 29.328113569209215 21.795318309007197 26-27 21.45361070308468 27.120893319233865 29.33434146940042 22.091154508281033 28-29 21.526596804370172 27.223486204626564 29.360209962234517 21.889707028768754 30-31 21.783867338869904 27.700629676240236 28.950797047339222 21.56470593755064 32-33 21.40378996330291 27.3037997799907 29.44374749169191 21.848662765014478 34-35 21.497246996526627 27.06904740594025 29.50033780955048 21.93336778798264 36-37 21.88661220329783 27.439591279316527 28.872990105356156 21.800806412029488 38-39 21.832764783498725 27.61477584748332 28.917760698024246 21.63469867099371 40-41 21.622955029275932 27.362416118282447 29.139435428468722 21.875193423972895 42-43 21.536964882243055 27.32742118498971 28.97171639011517 22.163897542652062 44-45 21.774009019803724 27.48059038715284 28.80237852570673 21.943022067336702 46-47 21.823218776436484 27.712708135435644 28.516467493770737 21.947605594357135 48-49 21.85718018530442 27.591840166999184 28.40559666694577 22.14538298075063 50-51 21.98838815760981 27.616363841096696 28.213720120456532 22.18152788083696 52-53 22.015311867509364 27.552356871248364 28.220523229459303 22.21180803178297 54-55 22.078470704405095 27.991653649931138 27.988856615725755 21.941019029938012 56-57 21.79524843438266 27.680948263168077 28.199482314082037 22.324320988367223 58-59 21.728480521092884 27.277363295404346 28.57192095256519 22.42223523093758 60-61 21.956664376106183 27.74416123620972 28.152654547869343 22.146519839814754 62-63 21.85838922589642 27.64619285749345 28.194465697894323 22.300952218715803 64-65 21.821414238239463 27.407759225521076 28.297107830540867 22.473718705698587 66-67 21.6767444109843 27.577223407603313 28.16974352459513 22.576288656817255 68-69 22.101213769796257 27.605205593063925 28.140919427643173 22.152661209496646 70-71 22.0604834394793 27.482255521572085 28.151847585789113 22.305413453159503 72-73 21.9078550606741 27.619661850586137 28.22762991164604 22.24485317709373 74-75 21.970986258253735 27.615638930478504 28.015308266720275 22.398066544547486 76-77 21.93923253712296 27.634391177684936 27.913120147596786 22.513256137595317 78-79 22.056365111491587 27.790195005615725 27.971623275944207 22.18181660694848 80-81 22.032924160312287 27.42538956370597 28.156444078083087 22.385242197898652 82-83 21.858342978593477 27.59758855864625 28.23433813665484 22.309730326105434 84-85 21.843501785771 27.545770306829237 28.0062162731811 22.604511634218664 86-87 21.98081842539873 27.69822548419509 27.974807852218 22.346148238188178 88-89 21.69305342490696 27.528950303700512 28.064673708950266 22.713322562442265 90-91 21.66916178470476 27.470870639799273 28.130783645114814 22.729183930381154 92-93 21.84703291023932 27.609437634686852 27.98207652071854 22.561452934355284 94-95 21.56021155741271 27.391466207494663 28.136922954491773 22.911399280600854 96-97 21.557975002788016 27.355385054581927 28.145376641815744 22.941263300814317 98-99 21.52141753022873 27.506567619138362 28.05469629430729 22.917318556325615 100 21.636990434503925 27.295986129597605 27.98914534183728 23.077878094061195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 12.0 2 43.0 3 84.5 4 65.0 5 58.5 6 144.5 7 306.5 8 526.5 9 654.0 10 585.5 11 509.5 12 591.5 13 734.5 14 850.5 15 1016.5 16 1234.5 17 1453.0 18 1722.0 19 2032.0 20 2441.5 21 3098.5 22 4106.5 23 5564.0 24 7482.5 25 10133.5 26 13801.0 27 18263.0 28 23888.0 29 30963.0 30 38608.5 31 46896.5 32 55618.0 33 64626.0 34 73298.0 35 80406.0 36 86502.0 37 92711.5 38 97284.5 39 99671.0 40 101771.5 41 103287.0 42 104381.0 43 104652.5 44 102895.5 45 101865.0 46 101200.5 47 97821.5 48 94613.5 49 92585.0 50 90350.0 51 86983.5 52 80685.0 53 75829.0 54 73534.0 55 70019.5 56 64191.0 57 58093.5 58 52269.0 59 45654.0 60 39345.5 61 33519.5 62 28398.0 63 23070.5 64 18419.0 65 14755.5 66 11113.0 67 8219.5 68 6354.5 69 4952.5 70 3626.0 71 2531.0 72 1902.0 73 1392.5 74 1016.0 75 656.0 76 344.5 77 212.0 78 127.0 79 75.0 80 45.0 81 20.0 82 8.0 83 2.5 84 3.0 85 3.5 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042695373741515066 2 0.24195248145656548 3 0.0020571735446038536 4 0.002273718128246364 5 7.218152788083696E-5 6 1.0827229182125544E-4 7 0.0 8 3.609076394041848E-4 9 0.0 10-11 7.57906042748788E-4 12-13 0.0 14-15 3.0677149349355706E-4 16-17 1.804538197020924E-5 18-19 5.23316077136068E-4 20-21 5.413614591062772E-5 22-23 3.609076394041848E-5 24-25 0.0014797213215571577 26-27 7.218152788083696E-5 28-29 2.8872611152334785E-4 30-31 1.0827229182125544E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.804538197020924E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0827229182125544E-4 70-71 1.804538197020924E-5 72-73 1.804538197020924E-4 74-75 5.413614591062772E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.609076394041848E-5 84-85 0.0 86-87 2.8872611152334785E-4 88-89 0.0018947651068719703 90-91 0.001732356669140087 92-93 4.6917993122544025E-4 94-95 2.7068072955313863E-4 96-97 1.0827229182125544E-4 98-99 3.609076394041848E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2770792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.07150471223957 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5668561200463 50.47392043529201 2 13.895273119261159 18.083726605157164 3 4.105895515429208 8.01530398140645 4 1.6757390253429971 4.361714395363623 5 0.8522507329529451 2.772861879267859 6 0.4828984526281038 1.8853757361433707 7 0.31069640835772516 1.4152237960367935 8 0.212885765501239 1.1082237674386077 9 0.14533224657533778 0.8511289171080707 >10 0.7062113980439125 7.937146056732719 >50 0.034229500592205864 1.509866257104343 >100 0.01145401005487785 1.2489233866198872 >500 1.1108208563294643E-4 0.05258572578243097 >1k 1.6662312839306186E-4 0.2839990605466446 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3459 0.12483795246990752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3020 0.10899410710006383 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.609076394041848E-5 0.0 12-13 0.0 0.0 0.0 7.218152788083696E-5 0.0 14-15 0.0 0.0 0.0 1.6240843773188316E-4 0.0 16-17 0.0 0.0 0.0 2.3458996561272013E-4 0.0 18-19 0.0 0.0 0.0 2.5263534758292937E-4 0.0 20-21 0.0 0.0 0.0 2.5263534758292937E-4 0.0 22-23 0.0 0.0 0.0 2.7068072955313863E-4 0.0 24-25 0.0 0.0 0.0 2.8872611152334785E-4 0.0 26-27 0.0 0.0 0.0 2.8872611152334785E-4 0.0 28-29 0.0 0.0 0.0 5.954976050169049E-4 0.0 30-31 0.0 0.0 0.0 0.0025804896217399213 0.0 32-33 0.0 0.0 0.0 0.006514382891245535 0.0 34-35 1.804538197020924E-5 0.0 0.0 0.012758085052937932 0.0 36-37 3.609076394041848E-5 0.0 0.0 0.024090584930229336 0.0 38-39 3.609076394041848E-5 0.0 0.0 0.044554048084446615 0.0 40-41 3.609076394041848E-5 0.0 0.0 0.07174843871355194 0.0 42-43 3.609076394041848E-5 0.0 0.0 0.10114436594302278 0.0 44-45 3.609076394041848E-5 0.0 0.0 0.1333734181418165 0.0 46-47 3.609076394041848E-5 0.0 0.0 0.17254994239914073 0.0 48-49 3.609076394041848E-5 0.0 0.0 0.21739271659511072 0.0 50-51 3.609076394041848E-5 0.0 0.0 0.26842505680686246 0.0 52-53 3.609076394041848E-5 0.0 0.0 0.32093711834017136 0.0 54-55 3.609076394041848E-5 0.0 0.0 0.37565071647384574 0.0 56-57 3.609076394041848E-5 0.0 0.0 0.44288780969484537 0.0 58-59 3.609076394041848E-5 0.0 0.0 0.5228649425868128 0.0 60-61 3.609076394041848E-5 0.0 0.0 0.6101865459406552 0.0 62-63 3.609076394041848E-5 0.0 0.0 0.7007021819032248 0.0 64-65 3.609076394041848E-5 0.0 0.0 0.7969562493323208 0.0 66-67 3.609076394041848E-5 0.0 0.0 0.9031172314630618 0.0 68-69 3.609076394041848E-5 0.0 0.0 1.026890506396727 0.0 70-71 3.609076394041848E-5 0.0 0.0 1.165659493747636 0.0 72-73 3.609076394041848E-5 0.0 0.0 1.315724890211896 0.0 74-75 3.609076394041848E-5 1.804538197020924E-5 0.0 1.4657000597663052 0.0 76-77 3.609076394041848E-5 3.609076394041848E-5 0.0 1.627909998296516 0.0 78-79 3.609076394041848E-5 3.609076394041848E-5 0.0 1.8147879739800028 0.0 80-81 3.609076394041848E-5 3.609076394041848E-5 0.0 2.030466379287944 0.0 82-83 3.609076394041848E-5 3.609076394041848E-5 0.0 2.2526050313412194 0.0 84-85 3.609076394041848E-5 3.609076394041848E-5 0.0 2.475952723986499 0.0 86-87 3.609076394041848E-5 3.609076394041848E-5 0.0 2.7091892859514535 0.0 88 3.609076394041848E-5 3.609076394041848E-5 0.0 2.903538049770607 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3500 0.0 50.21437 1 GTATCAA 6005 0.0 42.527855 1 TCAACGC 6925 0.0 36.243134 4 ATCAACG 7000 0.0 35.720528 3 CAACGCA 7105 0.0 35.389816 5 AACGCAG 7295 0.0 34.59693 6 TATCAAC 7555 0.0 33.355324 2 ACGCAGA 8485 0.0 29.523235 7 CGCAGAG 8720 0.0 28.620321 8 GTACATG 7080 0.0 26.420712 1 TACATGG 7180 0.0 25.060213 2 GCAGAGT 9865 0.0 25.012138 9 ACATGGG 6890 0.0 24.830555 3 GTGGTAT 1710 0.0 22.870161 1 TACACCG 390 0.0 21.691847 5 GAGTACT 6635 0.0 21.250105 12-13 GACGCGT 80 5.4185366E-5 20.562061 10-11 TGGTATC 1710 0.0 20.383625 2 AGAGTAC 9180 0.0 20.171682 10-11 CAGAGTA 10225 0.0 19.994686 10-11 >>END_MODULE